MD14G1022100.v1.1

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0001039
Physical Location & Seq
Forward (+)
2030996 .. 2031601
606 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1022100.v1.1.491

Sequence Viewer

Length: 606 bp
ATGACTCCAAAGTGCGGCACACGCTGCCTCTACTTCGTCGGGCAGGCCTTCCTCTGCCTGTGCCTAACCACTTTCATATTCTGGTTGATCTTCCTTCCCAAAGAGCCTAAATTCACCGTCTCCAATGCCTCTCTAACCCGATTCAACTTCACCAACACCGACAACACTCTCTACTACAATCTCATGCTCAACGTCACCATCCGAAACCCCAACAAAAGGGTTGGCATATACTACCGCCGCATCCAAGTCTTTGCCAACTACAGAAAAAAGAGGTTCGCTAATGTGACATTGCCTTTGGAGCCGTTTTACCAAGGCCGCAAGAACACGACCACTTTAAACCATGTACTCGTTGAAGGTCAGCAGTGGGTGGCATTTGGGGAACACGACCTTTCTCAGTTTAACTCGGAGACTGCTGCCGGTGTTTACAGTATTGATGTGGAGATTTATCTTCTGGTAAAGGCCAGGTACGGCAAGGTCAAGACATCAGATTACGGTTCTAGCAACATCGACTGCAAGCTGAAGGTTCCTGTGAGTTCTAACAACACATCCGTGAGTGGTTTCAATCCCACAAAATGCAGGAATGTTCATGTCCTCCGCGAATCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

202

Amino Acids

23.09

Weight (kDa)

9.62

Isoelectric Point (pI)

28.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 65 - 159 5.3e-08 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 597
AciI CCGC 5 cut(s) 15, 235, 238, 316, 595
AcsI RAATTY 1 cut(s) 110
AcuI CTGAAG 1 cut(s) 539
AfaI GTAC 2 cut(s) 345, 467
AfiI CCNNNNNNNGG 2 cut(s) 14, 216
AgsI TTSAA 3 cut(s) 145, 353, 562
AjnI CCWGG 1 cut(s) 461
AleI CACNNNNGTG 1 cut(s) 548
AluBI AGCT 1 cut(s) 517
AluI AGCT 1 cut(s) 517
Alw26I GTCTC 2 cut(s) 124, 401
AoxI GGCC 3 cut(s) 45, 313, 459
ApeKI GCWGC 2 cut(s) 24, 413
ApoI RAATTY 1 cut(s) 110
ArsI GACNNNNNNTTYG 2 cut(s) 277, 309
AsuHPI GGTGA 3 cut(s) 106, 142, 187
BbvI GCAGC 2 cut(s) 11, 400
BccI CCATC 1 cut(s) 206
BceAI ACGGC 2 cut(s) 286, 484
BciT130I CCWGG 1 cut(s) 463
BcoDI GTCTC 2 cut(s) 124, 401
BfaI CTAG 2 cut(s) 498, 604
BfmI CTRYAG 1 cut(s) 259
BisI GCNGC 5 cut(s) 16, 25, 238, 316, 414
BlsI GCNGC 5 cut(s) 17, 26, 239, 317, 415
Bme1390I CCNGG 1 cut(s) 463
BmiI GGNNCC 2 cut(s) 300, 525
BmrFI CCNGG 1 cut(s) 463
BmsI GCATC 1 cut(s) 249
BsaJI CCNNGG 1 cut(s) 310
Bsc4I CCNNNNNNNGG 2 cut(s) 14, 216
Bse118I RCCGGY 1 cut(s) 416
Bse3DI GCAATG 1 cut(s) 287
BseBI CCWGG 1 cut(s) 463
BseDI CCNNGG 1 cut(s) 310
BseGI GGATG 3 cut(s) 198, 240, 545
BseLI CCNNNNNNNGG 2 cut(s) 14, 216
BseMI GCAATG 1 cut(s) 287
BseMII CTCAG 1 cut(s) 407
BseXI GCAGC 2 cut(s) 11, 400
Bsh1236I CGCG 1 cut(s) 597
BshFI GGCC 3 cut(s) 47, 315, 461
BsiSI CCGG 1 cut(s) 417
BslI CCNNNNNNNGG 2 cut(s) 14, 216
BsmAI GTCTC 2 cut(s) 124, 401
BsmBI CGTCTC 1 cut(s) 124
BsnI GGCC 3 cut(s) 47, 315, 461
Bsp143I GATC 1 cut(s) 87
BspACI CCGC 5 cut(s) 15, 235, 238, 316, 595
BspANI GGCC 3 cut(s) 47, 315, 461
BspCNI CTCAG 1 cut(s) 406
BspFNI CGCG 1 cut(s) 597
BspLI GGNNCC 2 cut(s) 300, 525
BsrDI GCAATG 1 cut(s) 287
BsrFI RCCGGY 1 cut(s) 416
BssAI RCCGGY 1 cut(s) 416
BssECI CCNNGG 1 cut(s) 310
BssMI GATC 1 cut(s) 87
BssT1I CCWWGG 1 cut(s) 310
Bst2UI CCWGG 1 cut(s) 463
Bst4CI ACNGT 3 cut(s) 118, 428, 494
BstAPI GCANNNNNTGC 1 cut(s) 24
BstC8I GCNNGC 2 cut(s) 45, 515
BstDEI CTNAG 1 cut(s) 393
BstF5I GGATG 3 cut(s) 198, 240, 545
BstFNI CGCG 1 cut(s) 597
BstKTI GATC 1 cut(s) 90
BstMAI GTCTC 2 cut(s) 124, 401
BstMBI GATC 1 cut(s) 87
BstMWI GCNNNNNNNGC 3 cut(s) 21, 24, 298
BstNI CCWGG 1 cut(s) 463
BstSCI CCNGG 1 cut(s) 461
BstSFI CTRYAG 1 cut(s) 259
BstUI CGCG 1 cut(s) 597
BstV1I GCAGC 2 cut(s) 11, 400
BsuRI GGCC 3 cut(s) 47, 315, 461
BtsCI GGATG 3 cut(s) 198, 240, 545
BtsI GCAGTG 1 cut(s) 368
BtsIMutI CAGTG 1 cut(s) 368
Cac8I GCNNGC 2 cut(s) 45, 515
Cfr10I RCCGGY 1 cut(s) 416
Csp6I GTAC 2 cut(s) 344, 466
CviAII CATG 3 cut(s) 184, 341, 587
CviJI RGCY 6 cut(s) 47, 106, 301, 315, 461, 517
CviKI_1 RGCY 6 cut(s) 47, 106, 301, 315, 461, 517
CviQI GTAC 2 cut(s) 344, 466
DdeI CTNAG 1 cut(s) 393
DpnI GATC 1 cut(s) 89
DpnII GATC 1 cut(s) 87
DraI TTTAAA 1 cut(s) 336
Eco130I CCWWGG 1 cut(s) 310
Eco147I AGGCCT 1 cut(s) 47
Eco57I CTGAAG 1 cut(s) 539
EcoRII CCWGG 1 cut(s) 461
EcoT14I CCWWGG 1 cut(s) 310
ErhI CCWWGG 1 cut(s) 310
Esp3I CGTCTC 1 cut(s) 124
FaeI CATG 3 cut(s) 187, 344, 590
FaiI YATR 6 cut(s) 77, 185, 227, 229, 342, 588
FatI CATG 3 cut(s) 183, 340, 586
Fnu4HI GCNGC 5 cut(s) 16, 25, 238, 316, 414
FokI GGATG 3 cut(s) 185, 227, 532
Fsp4HI GCNGC 5 cut(s) 16, 25, 238, 316, 414
FspBI CTAG 2 cut(s) 498, 604
GluI GCNGC 5 cut(s) 16, 25, 238, 316, 414
HaeIII GGCC 3 cut(s) 47, 315, 461
HapII CCGG 1 cut(s) 417
Hin1II CATG 3 cut(s) 187, 344, 590
HinfI GANTC 3 cut(s) 4, 141, 599
HpaII CCGG 1 cut(s) 417
HphI GGTGA 3 cut(s) 106, 142, 187
Hpy166II GTNNAC 1 cut(s) 424
Hpy188I TCNGA 3 cut(s) 203, 406, 487
Hpy188III TCNNGA 1 cut(s) 478
Hpy8I GTNNAC 1 cut(s) 424
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 4 cut(s) 58, 104, 347, 514
HpyCH4III ACNGT 3 cut(s) 118, 428, 494
HpyCH4IV ACGT 1 cut(s) 192
HpyCH4V TGCA 2 cut(s) 513, 576
HpyF10VI GCNNNNNNNGC 3 cut(s) 21, 24, 298
HpyF3I CTNAG 1 cut(s) 393
HpySE526I ACGT 1 cut(s) 192
Hsp92II CATG 3 cut(s) 187, 344, 590
Kzo9I GATC 1 cut(s) 87
LmnI GCTCC 1 cut(s) 298
LpnPI CCDG 9 cut(s) 29, 67, 71, 430, 437, 448, 475, 540, 562
Lsp1109I GCAGC 2 cut(s) 11, 400
LweI GCATC 1 cut(s) 249
MaeI CTAG 2 cut(s) 498, 604
MaeII ACGT 1 cut(s) 192
MaeIII GTNAC 2 cut(s) 193, 283
MalI GATC 1 cut(s) 89
MboI GATC 1 cut(s) 87
MboII GAAGA 2 cut(s) 82, 440
MluCI AATT 1 cut(s) 110
MnlI CCTC 5 cut(s) 38, 62, 139, 264, 602
MseI TTAA 2 cut(s) 335, 399
MslI CAYNNNNRTG 1 cut(s) 548
MspI CCGG 1 cut(s) 417
MspR9I CCNGG 1 cut(s) 463
MvaI CCWGG 1 cut(s) 463
MvnI CGCG 1 cut(s) 597
MwoI GCNNNNNNNGC 3 cut(s) 21, 24, 298
NdeII GATC 1 cut(s) 87
NlaIII CATG 3 cut(s) 187, 344, 590
NlaIV GGNNCC 2 cut(s) 300, 525
NmuCI GTSAC 2 cut(s) 193, 283
OliI CACNNNNGTG 1 cut(s) 548
PceI AGGCCT 1 cut(s) 47
PfeI GAWTC 2 cut(s) 141, 599
PkrI GCNGC 5 cut(s) 17, 26, 239, 317, 415
Psp6I CCWGG 1 cut(s) 461
PspGI CCWGG 1 cut(s) 461
PspN4I GGNNCC 2 cut(s) 300, 525
RsaI GTAC 2 cut(s) 345, 467
RsaNI GTAC 2 cut(s) 344, 466
RseI CAYNNNNRTG 1 cut(s) 548
SaqAI TTAA 2 cut(s) 335, 399
SatI GCNGC 5 cut(s) 16, 25, 238, 316, 414
Sau3AI GATC 1 cut(s) 87
ScrFI CCNGG 1 cut(s) 463
SetI ASST 8 cut(s) 195, 275, 358, 390, 467, 477, 519, 525
SfaNI GCATC 1 cut(s) 249
SfcI CTRYAG 1 cut(s) 259
SmiMI CAYNNNNRTG 1 cut(s) 548
Sse9I AATT 1 cut(s) 110
SseBI AGGCCT 1 cut(s) 47
SsiI CCGC 5 cut(s) 15, 235, 238, 316, 595
SspMI CTAG 2 cut(s) 498, 604
StuI AGGCCT 1 cut(s) 47
StyD4I CCNGG 1 cut(s) 461
StyI CCWWGG 1 cut(s) 310
TaaI ACNGT 3 cut(s) 118, 428, 494
TaiI ACGT 1 cut(s) 195
TaqI TCGA 1 cut(s) 507
TasI AATT 1 cut(s) 110
TatI WGTACW 1 cut(s) 343
TauI GCSGC 3 cut(s) 18, 240, 318
TfiI GAWTC 2 cut(s) 141, 599
Tru1I TTAA 2 cut(s) 335, 399
Tru9I TTAA 2 cut(s) 335, 399
TscAI CASTG 1 cut(s) 368
TseFI GTSAC 2 cut(s) 193, 283
TseI GCWGC 2 cut(s) 24, 413
Tsp45I GTSAC 2 cut(s) 193, 283
TspDTI ATGAA 2 cut(s) 64, 575
TspGWI ACGGA 1 cut(s) 538
TspRI CASTG 1 cut(s) 368
XapI RAATTY 1 cut(s) 110
XspI CTAG 2 cut(s) 498, 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.