Rw0G008060

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00301
OG0001039
Physical Location & Seq
Forward (+)
10654 .. 11223
570 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G008060.1

Sequence Viewer

Length: 570 bp
ATGACTTGCTCCATGACATGTAACGGAAAGAAACTAACAAGCTACACCATTCTCTCTATATTTACCGCCATCGCCGTCTTAGCCGGAGTCATAGCATTCGTAGTCATCTTCTCTAGATCTCCCAAGCTCGAAGTAACAGTCACCAATGCGTCTATGACCCAATTCAATCTCAACAGCAACAACACTCTCTACTACAACCTCGCCGTCGATGTCGCCATCAGAAACCCTAGCCCAACCTTCAGGGTACACTACAATCGCATCGAAGTCGCGGTGAAATATAGGGAGCAGAAATTTGCTTTGGTGACTTTGAGTTTGCCACCCTTTGACCAAGGAACCAAGAACATAACCATTCTGCATCCAGTTCTTCAAGGGCAACCCTCTAGCCTGGAGACCGATGCAGGCGTTTACAGTATTGACCTGCAGTTTTCTTTTCGGATAAGCCGCCGGGTTCTCAAAGGAATCGTTTACAAACCAGTAGGGATGTGTATGTTGAAGGTTCCGTCCAGTTCTAATGAAACAACTCCCGGTAGTAGGTTCAAGGCAACAGAATGTTTGGTGACGAGACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

189

Amino Acids

20.98

Weight (kDa)

9.69

Isoelectric Point (pI)

44.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 71 - 119 2.4e-06 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 426
AccII CGCG 1 cut(s) 269
AciI CCGC 3 cut(s) 66, 269, 442
AcsI RAATTY 1 cut(s) 290
AcuI CTGAAG 1 cut(s) 223
AfaI GTAC 1 cut(s) 246
AfiI CCNNNNNNNGG 1 cut(s) 531
AflIII ACRYGT 1 cut(s) 17
AgsI TTSAA 4 cut(s) 166, 368, 493, 538
AjnI CCWGG 1 cut(s) 384
AjuI GAANNNNNNNTTGG 2 cut(s) 281, 313
AluBI AGCT 2 cut(s) 42, 127
AluI AGCT 2 cut(s) 42, 127
Alw26I GTCTC 2 cut(s) 383, 556
ApoI RAATTY 1 cut(s) 290
ArsI GACNNNNNNTTYG 4 cut(s) 123, 155, 295, 327
AsuC2I CCSGG 2 cut(s) 446, 525
AsuHPI GGTGA 4 cut(s) 133, 283, 313, 568
BccI CCATC 2 cut(s) 77, 224
BceAI ACGGC 2 cut(s) 59, 188
BcgI CGANNNNNNTGC 2 cut(s) 247, 281
BciT130I CCWGG 1 cut(s) 386
BcnI CCSGG 2 cut(s) 446, 525
BcoDI GTCTC 2 cut(s) 383, 556
BfaI CTAG 3 cut(s) 114, 228, 381
BfmI CTRYAG 1 cut(s) 419
BfuAI ACCTGC 1 cut(s) 426
BglII AGATCT 1 cut(s) 116
BisI GCNGC 1 cut(s) 442
BlsI GCNGC 1 cut(s) 443
Bme1390I CCNGG 3 cut(s) 386, 446, 525
BmiI GGNNCC 2 cut(s) 334, 498
BmrFI CCNGG 3 cut(s) 386, 446, 525
BmsI GCATC 3 cut(s) 267, 364, 385
BpmI CTGGAG 1 cut(s) 407
BpuMI CCSGG 2 cut(s) 446, 525
BsaI GGTCTC 1 cut(s) 383
BsaJI CCNNGG 1 cut(s) 328
Bsc4I CCNNNNNNNGG 1 cut(s) 531
Bse1I ACTGG 3 cut(s) 359, 473, 504
BseBI CCWGG 1 cut(s) 386
BseDI CCNNGG 1 cut(s) 328
BseGI GGATG 2 cut(s) 355, 486
BseLI CCNNNNNNNGG 1 cut(s) 531
BseNI ACTGG 3 cut(s) 359, 473, 504
Bsh1236I CGCG 1 cut(s) 269
BsiSI CCGG 3 cut(s) 84, 445, 525
BslI CCNNNNNNNGG 1 cut(s) 531
BsmAI GTCTC 2 cut(s) 383, 556
BsmI GAATGC 1 cut(s) 95
Bso31I GGTCTC 1 cut(s) 383
Bsp143I GATC 1 cut(s) 116
BspACI CCGC 3 cut(s) 66, 269, 442
BspFNI CGCG 1 cut(s) 269
BspLI GGNNCC 2 cut(s) 334, 498
BspMAI CTGCAG 1 cut(s) 423
BspMI ACCTGC 1 cut(s) 426
BspTNI GGTCTC 1 cut(s) 383
BsrI ACTGG 3 cut(s) 359, 473, 504
BssECI CCNNGG 1 cut(s) 328
BssMI GATC 1 cut(s) 116
BssT1I CCWWGG 1 cut(s) 328
Bst2UI CCWGG 1 cut(s) 386
Bst4CI ACNGT 2 cut(s) 139, 410
BstC8I GCNNGC 1 cut(s) 400
BstDEI CTNAG 1 cut(s) 79
BstF5I GGATG 2 cut(s) 355, 486
BstFNI CGCG 1 cut(s) 269
BstKTI GATC 1 cut(s) 119
BstMAI GTCTC 2 cut(s) 383, 556
BstMBI GATC 1 cut(s) 116
BstMWI GCNNNNNNNGC 1 cut(s) 80
BstNI CCWGG 1 cut(s) 386
BstNSI RCATGY 1 cut(s) 21
BstSCI CCNGG 3 cut(s) 384, 444, 523
BstSFI CTRYAG 1 cut(s) 419
BstUI CGCG 1 cut(s) 269
BstX2I RGATCY 1 cut(s) 116
BstYI RGATCY 1 cut(s) 116
BtgZI GCGATG 1 cut(s) 55
BtsCI GGATG 2 cut(s) 355, 486
BveI ACCTGC 1 cut(s) 426
Cac8I GCNNGC 1 cut(s) 400
CseI GACGC 1 cut(s) 138
Csp6I GTAC 1 cut(s) 245
CviAII CATG 2 cut(s) 13, 18
CviJI RGCY 6 cut(s) 42, 83, 127, 231, 384, 441
CviKI_1 RGCY 6 cut(s) 42, 83, 127, 231, 384, 441
CviQI GTAC 1 cut(s) 245
DdeI CTNAG 1 cut(s) 79
DpnI GATC 1 cut(s) 118
DpnII GATC 1 cut(s) 116
Eco130I CCWWGG 1 cut(s) 328
Eco31I GGTCTC 1 cut(s) 383
Eco57I CTGAAG 1 cut(s) 223
EcoRII CCWGG 1 cut(s) 384
EcoT14I CCWWGG 1 cut(s) 328
ErhI CCWWGG 1 cut(s) 328
FaeI CATG 2 cut(s) 16, 21
FaiI YATR 8 cut(s) 14, 19, 59, 92, 155, 279, 344, 488
FatI CATG 2 cut(s) 12, 17
Fnu4HI GCNGC 1 cut(s) 442
FokI GGATG 2 cut(s) 342, 493
Fsp4HI GCNGC 1 cut(s) 442
FspBI CTAG 3 cut(s) 114, 228, 381
GluI GCNGC 1 cut(s) 442
GsuI CTGGAG 1 cut(s) 407
HapII CCGG 3 cut(s) 84, 445, 525
HgaI GACGC 1 cut(s) 138
Hin1II CATG 2 cut(s) 16, 21
HinfI GANTC 2 cut(s) 87, 459
HpaII CCGG 3 cut(s) 84, 445, 525
HphI GGTGA 4 cut(s) 133, 283, 313, 568
Hpy166II GTNNAC 3 cut(s) 247, 406, 466
Hpy188I TCNGA 2 cut(s) 221, 435
Hpy188III TCNNGA 1 cut(s) 114
Hpy8I GTNNAC 3 cut(s) 247, 406, 466
Hpy99I CGWCG 1 cut(s) 209
HpyAV CCTTC 2 cut(s) 247, 487
HpyCH4III ACNGT 2 cut(s) 139, 410
HpyCH4V TGCA 3 cut(s) 355, 398, 421
HpyF10VI GCNNNNNNNGC 1 cut(s) 80
HpyF3I CTNAG 1 cut(s) 79
Hsp92II CATG 2 cut(s) 16, 21
Kzo9I GATC 1 cut(s) 116
LmnI GCTCC 2 cut(s) 14, 283
LweI GCATC 3 cut(s) 267, 364, 385
MaeI CTAG 3 cut(s) 114, 228, 381
MaeIII GTNAC 5 cut(s) 20, 133, 139, 301, 556
MalI GATC 1 cut(s) 118
MboI GATC 1 cut(s) 116
MboII GAAGA 2 cut(s) 100, 356
MflI RGATCY 1 cut(s) 116
MluCI AATT 2 cut(s) 161, 290
MlyI GAGTC 1 cut(s) 96
MnlI CCTC 2 cut(s) 209, 388
MspI CCGG 3 cut(s) 84, 445, 525
MspR9I CCNGG 3 cut(s) 386, 446, 525
Mva1269I GAATGC 1 cut(s) 95
MvaI CCWGG 1 cut(s) 386
MvnI CGCG 1 cut(s) 269
MwoI GCNNNNNNNGC 1 cut(s) 80
NciI CCSGG 2 cut(s) 446, 525
NdeII GATC 1 cut(s) 116
NlaIII CATG 2 cut(s) 16, 21
NlaIV GGNNCC 2 cut(s) 334, 498
NmuCI GTSAC 3 cut(s) 139, 301, 556
NspI RCATGY 1 cut(s) 21
PciI ACATGT 1 cut(s) 17
PctI GAATGC 1 cut(s) 95
PfeI GAWTC 1 cut(s) 459
PkrI GCNGC 1 cut(s) 443
PleI GAGTC 1 cut(s) 95
PpsI GAGTC 1 cut(s) 95
PscI ACATGT 1 cut(s) 17
Psp6I CCWGG 1 cut(s) 384
PspGI CCWGG 1 cut(s) 384
PspN4I GGNNCC 2 cut(s) 334, 498
PstI CTGCAG 1 cut(s) 423
PsuI RGATCY 1 cut(s) 116
RsaI GTAC 1 cut(s) 246
RsaNI GTAC 1 cut(s) 245
SatI GCNGC 1 cut(s) 442
Sau3AI GATC 1 cut(s) 116
SchI GAGTC 1 cut(s) 96
ScrFI CCNGG 3 cut(s) 386, 446, 525
SetI ASST 7 cut(s) 44, 129, 201, 239, 420, 498, 536
SfaNI GCATC 3 cut(s) 267, 364, 385
SfcI CTRYAG 1 cut(s) 419
Sse9I AATT 2 cut(s) 161, 290
SsiI CCGC 3 cut(s) 66, 269, 442
SspMI CTAG 3 cut(s) 114, 228, 381
StyD4I CCNGG 3 cut(s) 384, 444, 523
StyI CCWWGG 1 cut(s) 328
TaaI ACNGT 2 cut(s) 139, 410
TaqI TCGA 3 cut(s) 129, 207, 261
TaqII GACCGA 1 cut(s) 407
TasI AATT 2 cut(s) 161, 290
TauI GCSGC 1 cut(s) 444
TfiI GAWTC 1 cut(s) 459
TseFI GTSAC 3 cut(s) 139, 301, 556
Tsp45I GTSAC 3 cut(s) 139, 301, 556
TspDTI ATGAA 1 cut(s) 528
TspGWI ACGGA 2 cut(s) 39, 489
XapI RAATTY 1 cut(s) 290
XbaI TCTAGA 1 cut(s) 113
XceI RCATGY 1 cut(s) 21
XspI CTAG 3 cut(s) 114, 228, 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.