Rroxscaffold_6G00408310

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001039
Physical Location & Seq
Reverse (-)
30756088 .. 30757716
1629 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00408310.1

Sequence Viewer

Length: 864 bp
ATGGCTCTCCCTTGCATGCCTTTGCTTGAGTTTACATGCATTAATATTCCTTTGGCCATGACTTGCTCCATGACATGTAACGGAAAGAAACTAACAAGCTGCACCATTCTCTCTATATTTACCGCCATCGCTGTCTTAGCCGGAGTCATAGCATTCGTAGTCATCTTCTCTAGATCTCCCAAGCTCGAAGTAACAGTCACCAATGCGTCTATGACCCAATTCAATCTCAACAGCAACAACAACACTCTCTACTACATCCTCGCCCTCGATGTCGCCATCAGAAACCCTAGCCCAACCTTCAGGGTACATTACAATCGCATCGAAGTCGCGGCGAAATATAGGGAGCAGAAATTTGCTTTGGTGACTTTGAGTTCGCCACCCTTTGACCAAGGAACCAAAAACATAACCATTCTGCATCCAGTTCTTCAAGGGCAACCCTCTAGCCTGGAGACCGATGCGGGCATTTACAGTATTGACCTGCAGTTTTCTTTTCGGATAAGCCGCCGGGTTCTCAAAGGAATCGTTTACAAACCAGTAGGGATGTGTATGTTGAAGGTTCCGTCGAGTTATAATGAAACAACTCCCGGTAGTAGTTTCAAGGCAACAGAATGTTTGTCACGCCACGAGACAACAGATAAGCCGTGGTCGCTCCAAGAGCTTCCCTTAAAAAGTCAAGTGACCAGTAACTACTTCCTTGTAATAACTTTGGAAGTCGCTCTCCTCAAACAAACTTTGGAATTTTACACCTGGAGATTTCTCAAAATACTCTTTATAATTCAGCTTGACATTGACCTTTTCGTCACAGCTGTTCTCTGCCAAATGGATCCACCGGTTAGTTCCCTTCCTGTAGACAAGCCAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

287

Amino Acids

32.1

Weight (kDa)

8.73

Isoelectric Point (pI)

47.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 91 - 139 1e-05 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 570, 773
AasI GACNNNNNNGTC 1 cut(s) 797
Acc36I ACCTGC 1 cut(s) 486
AccI GTMKAC 1 cut(s) 849
AccII CGCG 1 cut(s) 329
AciI CCGC 4 cut(s) 123, 329, 458, 502
AclWI GGATC 2 cut(s) 818, 831
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 2 cut(s) 350, 737
AcuI CTGAAG 1 cut(s) 283
AfaI GTAC 1 cut(s) 306
AflIII ACRYGT 1 cut(s) 74
AgeI ACCGGT 1 cut(s) 829
AgsI TTSAA 4 cut(s) 223, 428, 553, 598
AjnI CCWGG 2 cut(s) 444, 746
AjuI GAANNNNNNNTTGG 2 cut(s) 341, 373
AluBI AGCT 5 cut(s) 99, 184, 658, 781, 806
AluI AGCT 5 cut(s) 99, 184, 658, 781, 806
Alw26I GTCTC 2 cut(s) 443, 620
AlwI GGATC 2 cut(s) 818, 831
AoxI GGCC 1 cut(s) 54
ApeKI GCWGC 1 cut(s) 99
ApoI RAATTY 2 cut(s) 350, 737
ArsI GACNNNNNNTTYG 4 cut(s) 180, 212, 355, 387
AseI ATTAAT 1 cut(s) 42
AsiGI ACCGGT 1 cut(s) 829
AsuC2I CCSGG 2 cut(s) 506, 585
AsuHPI GGTGA 2 cut(s) 190, 373
BalI TGGCCA 1 cut(s) 56
BamHI GGATCC 1 cut(s) 823
BauI CACGAG 1 cut(s) 623
BbvI GCAGC 1 cut(s) 86
BccI CCATC 2 cut(s) 134, 284
BceAI ACGGC 1 cut(s) 625
BcgI CGANNNNNNTGC 2 cut(s) 307, 341
BciT130I CCWGG 2 cut(s) 446, 748
BcnI CCSGG 2 cut(s) 506, 585
BcoDI GTCTC 2 cut(s) 443, 620
BfaI CTAG 3 cut(s) 171, 288, 441
BfmI CTRYAG 2 cut(s) 479, 846
BfuAI ACCTGC 1 cut(s) 486
BglII AGATCT 1 cut(s) 173
BisI GCNGC 3 cut(s) 100, 330, 502
BlsI GCNGC 3 cut(s) 101, 331, 503
Bme1390I CCNGG 4 cut(s) 446, 506, 585, 748
BmiI GGNNCC 3 cut(s) 394, 558, 825
BmrFI CCNGG 4 cut(s) 446, 506, 585, 748
BmsI GCATC 3 cut(s) 327, 424, 445
BpmI CTGGAG 2 cut(s) 467, 769
BpuEI CTTGAG 1 cut(s) 47
BpuMI CCSGG 2 cut(s) 506, 585
BsaI GGTCTC 1 cut(s) 443
BsaJI CCNNGG 2 cut(s) 388, 641
BsaWI WCCGGW 1 cut(s) 829
Bse118I RCCGGY 1 cut(s) 829
Bse1I ACTGG 3 cut(s) 419, 533, 681
BseBI CCWGG 2 cut(s) 446, 748
BseDI CCNNGG 2 cut(s) 388, 641
BseGI GGATG 3 cut(s) 255, 415, 546
BseNI ACTGG 3 cut(s) 419, 533, 681
BseRI GAGGAG 1 cut(s) 710
BseXI GCAGC 1 cut(s) 86
BsgI GTGCAG 1 cut(s) 85
Bsh1236I CGCG 1 cut(s) 329
BshFI GGCC 1 cut(s) 56
BshTI ACCGGT 1 cut(s) 829
BsiSI CCGG 4 cut(s) 141, 505, 585, 830
BsmAI GTCTC 2 cut(s) 443, 620
BsmI GAATGC 1 cut(s) 152
BsnI GGCC 1 cut(s) 56
Bso31I GGTCTC 1 cut(s) 443
Bsp143I GATC 2 cut(s) 173, 823
BspACI CCGC 4 cut(s) 123, 329, 458, 502
BspANI GGCC 1 cut(s) 56
BspFNI CGCG 1 cut(s) 329
BspLI GGNNCC 3 cut(s) 394, 558, 825
BspMAI CTGCAG 1 cut(s) 483
BspMI ACCTGC 1 cut(s) 486
BspPI GGATC 2 cut(s) 818, 831
BspTNI GGTCTC 1 cut(s) 443
BsrFI RCCGGY 1 cut(s) 829
BsrI ACTGG 3 cut(s) 419, 533, 681
BssAI RCCGGY 1 cut(s) 829
BssECI CCNNGG 2 cut(s) 388, 641
BssMI GATC 2 cut(s) 173, 823
BssSI CACGAG 1 cut(s) 623
BssT1I CCWWGG 1 cut(s) 388
Bst2BI CACGAG 1 cut(s) 623
Bst2UI CCWGG 2 cut(s) 446, 748
Bst4CI ACNGT 2 cut(s) 196, 470
BstC8I GCNNGC 2 cut(s) 17, 460
BstDEI CTNAG 1 cut(s) 136
BstDSI CCRYGG 1 cut(s) 641
BstF5I GGATG 3 cut(s) 255, 415, 546
BstFNI CGCG 1 cut(s) 329
BstKTI GATC 2 cut(s) 176, 826
BstMAI GTCTC 2 cut(s) 443, 620
BstMBI GATC 2 cut(s) 173, 823
BstMWI GCNNNNNNNGC 3 cut(s) 137, 646, 655
BstNI CCWGG 2 cut(s) 446, 748
BstNSI RCATGY 3 cut(s) 19, 39, 78
BstSCI CCNGG 4 cut(s) 444, 504, 583, 746
BstSFI CTRYAG 2 cut(s) 479, 846
BstUI CGCG 1 cut(s) 329
BstV1I GCAGC 1 cut(s) 86
BstX2I RGATCY 2 cut(s) 173, 823
BstYI RGATCY 2 cut(s) 173, 823
BsuRI GGCC 1 cut(s) 56
BtgI CCRYGG 1 cut(s) 641
BtgZI GCGATG 1 cut(s) 112
BtsCI GGATG 3 cut(s) 255, 415, 546
BveI ACCTGC 1 cut(s) 486
Cac8I GCNNGC 2 cut(s) 17, 460
Cfr10I RCCGGY 1 cut(s) 829
CseI GACGC 1 cut(s) 195
Csp6I GTAC 1 cut(s) 305
CspAI ACCGGT 1 cut(s) 829
CviAII CATG 5 cut(s) 16, 36, 58, 70, 75
CviQI GTAC 1 cut(s) 305
DdeI CTNAG 1 cut(s) 136
DpnI GATC 2 cut(s) 175, 825
DpnII GATC 2 cut(s) 173, 823
DrdI GACNNNNNNGTC 1 cut(s) 797
DseDI GACNNNNNNGTC 1 cut(s) 797
EaeI YGGCCR 1 cut(s) 54
Eco130I CCWWGG 1 cut(s) 388
Eco31I GGTCTC 1 cut(s) 443
Eco57I CTGAAG 1 cut(s) 283
EcoRII CCWGG 2 cut(s) 444, 746
EcoT14I CCWWGG 1 cut(s) 388
EcoT22I ATGCAT 1 cut(s) 41
ErhI CCWWGG 1 cut(s) 388
FaeI CATG 5 cut(s) 19, 39, 61, 73, 78
FatI CATG 5 cut(s) 15, 35, 57, 69, 74
FauI CCCGC 1 cut(s) 451
FblI GTMKAC 1 cut(s) 849
Fnu4HI GCNGC 3 cut(s) 100, 330, 502
FokI GGATG 3 cut(s) 242, 402, 553
Fsp4HI GCNGC 3 cut(s) 100, 330, 502
FspBI CTAG 3 cut(s) 171, 288, 441
GluI GCNGC 3 cut(s) 100, 330, 502
GsuI CTGGAG 2 cut(s) 467, 769
HaeIII GGCC 1 cut(s) 56
HapII CCGG 4 cut(s) 141, 505, 585, 830
HgaI GACGC 1 cut(s) 195
Hin1II CATG 5 cut(s) 19, 39, 61, 73, 78
HinfI GANTC 2 cut(s) 144, 519
HpaII CCGG 4 cut(s) 141, 505, 585, 830
HphI GGTGA 2 cut(s) 190, 373
Hpy166II GTNNAC 3 cut(s) 33, 526, 850
Hpy188I TCNGA 2 cut(s) 281, 495
Hpy188III TCNNGA 1 cut(s) 171
Hpy8I GTNNAC 3 cut(s) 33, 526, 850
Hpy99I CGWCG 1 cut(s) 565
HpyAV CCTTC 3 cut(s) 307, 547, 851
HpyCH4III ACNGT 2 cut(s) 196, 470
HpyCH4V TGCA 5 cut(s) 15, 39, 102, 415, 481
HpyF10VI GCNNNNNNNGC 3 cut(s) 137, 646, 655
HpyF3I CTNAG 1 cut(s) 136
Hsp92II CATG 5 cut(s) 19, 39, 61, 73, 78
Kzo9I GATC 2 cut(s) 173, 823
LmnI GCTCC 3 cut(s) 71, 343, 654
Lsp1109I GCAGC 1 cut(s) 86
LweI GCATC 3 cut(s) 327, 424, 445
MaeI CTAG 3 cut(s) 171, 288, 441
MaeIII GTNAC 8 cut(s) 77, 190, 196, 361, 615, 676, 683, 799
MalI GATC 2 cut(s) 175, 825
MboI GATC 2 cut(s) 173, 823
MboII GAAGA 2 cut(s) 157, 416
MflI RGATCY 2 cut(s) 173, 823
MlsI TGGCCA 1 cut(s) 56
MluCI AATT 4 cut(s) 218, 350, 737, 774
MluNI TGGCCA 1 cut(s) 56
MlyI GAGTC 1 cut(s) 153
MnlI CCTC 4 cut(s) 269, 275, 448, 731
Mox20I TGGCCA 1 cut(s) 56
Mph1103I ATGCAT 1 cut(s) 41
MscI TGGCCA 1 cut(s) 56
MseI TTAA 2 cut(s) 42, 665
Msp20I TGGCCA 1 cut(s) 56
MspA1I CMGCKG 1 cut(s) 806
MspI CCGG 4 cut(s) 141, 505, 585, 830
MspR9I CCNGG 4 cut(s) 446, 506, 585, 748
Mva1269I GAATGC 1 cut(s) 152
MvaI CCWGG 2 cut(s) 446, 748
MvnI CGCG 1 cut(s) 329
MwoI GCNNNNNNNGC 3 cut(s) 137, 646, 655
NciI CCSGG 2 cut(s) 506, 585
NdeII GATC 2 cut(s) 173, 823
NlaIII CATG 5 cut(s) 19, 39, 61, 73, 78
NlaIV GGNNCC 3 cut(s) 394, 558, 825
NmuCI GTSAC 5 cut(s) 196, 361, 615, 676, 799
NsiI ATGCAT 1 cut(s) 41
NspI RCATGY 3 cut(s) 19, 39, 78
PaeI GCATGC 1 cut(s) 19
PciI ACATGT 1 cut(s) 74
PctI GAATGC 1 cut(s) 152
PfeI GAWTC 1 cut(s) 519
PinAI ACCGGT 1 cut(s) 829
PkrI GCNGC 3 cut(s) 101, 331, 503
PleI GAGTC 1 cut(s) 152
PpsI GAGTC 1 cut(s) 152
PscI ACATGT 1 cut(s) 74
PshBI ATTAAT 1 cut(s) 42
PsiI TTATAA 2 cut(s) 570, 773
Psp6I CCWGG 2 cut(s) 444, 746
PspGI CCWGG 2 cut(s) 444, 746
PspN4I GGNNCC 3 cut(s) 394, 558, 825
PstI CTGCAG 1 cut(s) 483
PsuI RGATCY 2 cut(s) 173, 823
PvuII CAGCTG 1 cut(s) 806
RsaI GTAC 1 cut(s) 306
RsaNI GTAC 1 cut(s) 305
SaqAI TTAA 2 cut(s) 42, 665
SatI GCNGC 3 cut(s) 100, 330, 502
Sau3AI GATC 2 cut(s) 173, 823
SchI GAGTC 1 cut(s) 153
ScrFI CCNGG 4 cut(s) 446, 506, 585, 748
SfaNI GCATC 3 cut(s) 327, 424, 445
SfcI CTRYAG 2 cut(s) 479, 846
SmlI CTYRAG 1 cut(s) 26
SmoI CTYRAG 1 cut(s) 26
SphI GCATGC 1 cut(s) 19
Sse9I AATT 4 cut(s) 218, 350, 737, 774
SsiI CCGC 4 cut(s) 123, 329, 458, 502
SspI AATATT 1 cut(s) 46
SspMI CTAG 3 cut(s) 171, 288, 441
StyD4I CCNGG 4 cut(s) 444, 504, 583, 746
StyI CCWWGG 1 cut(s) 388
TaaI ACNGT 2 cut(s) 196, 470
TaqI TCGA 4 cut(s) 186, 267, 321, 563
TaqII GACCGA 1 cut(s) 467
TasI AATT 4 cut(s) 218, 350, 737, 774
TauI GCSGC 2 cut(s) 332, 504
TfiI GAWTC 1 cut(s) 519
Tru1I TTAA 2 cut(s) 42, 665
Tru9I TTAA 2 cut(s) 42, 665
TseFI GTSAC 5 cut(s) 196, 361, 615, 676, 799
TseI GCWGC 1 cut(s) 99
Tsp45I GTSAC 5 cut(s) 196, 361, 615, 676, 799
TspDTI ATGAA 1 cut(s) 588
TspGWI ACGGA 2 cut(s) 96, 549
VspI ATTAAT 1 cut(s) 42
XapI RAATTY 2 cut(s) 350, 737
XbaI TCTAGA 1 cut(s) 170
XceI RCATGY 3 cut(s) 19, 39, 78
XmiI GTMKAC 1 cut(s) 849
XspI CTAG 3 cut(s) 171, 288, 441
Zsp2I ATGCAT 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.