Prupe.7G111500_v2.0.a1

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0001039
Physical Location & Seq
Reverse (-)
13831859 .. 13832458
600 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G111500.1

Sequence Viewer

Length: 600 bp
ATGACTTCAAGGTTTGGTGCCCGCTGCCTCTTCTGCTTCTGCAACACCATCCTCTGCCTCATCCTAGCCCTTTTCATATTCTGGTTGATCTTCCTTCCCAAGGAGCCCGAATTCACCGTCTCCAATGCCTCACTGACCCAATTCAGTTTCTACAATAACACTCTCTACTACAACCTTGCGCTCAACATCACCATCCAAAACCCCAACAAAAGGGTTGGCATATACTACCGTCACATCCAAGTCATTGCTAACTACAGAAAGGAGAGGTTTTCAATGGTTAATTTGACTTCGCCGCCATTTTACCAAGGCCACAAGAACACAACCTTTTTGCATGATGTACTTGTTGAAGGGCAGGAGTTGGTGGAGTTTGGGGAACATGAACTTTCTCAGTTTAACTCGGAGACTGCTGCCGGCGTTTACAATATTGATGTGAAGCTAGCTCTGTGGGTAAGAGCCAAGTATGGAAAGTTCAAGACAGCTGATTATGGTTCTAGCAAGATCGACTGCAAGCTGAAGGTTCCTTTGAGTTTTAGTGAAACACCTGCAACTGGTTTCAATACCACAAAGTGCGGCAATGTTTATGTCCTCACAAATCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

200

Amino Acids

22.8

Weight (kDa)

8.85

Isoelectric Point (pI)

32.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 550
Acc36I ACCTGC 1 cut(s) 550
AccB1I GGYRCC 1 cut(s) 17
AciI CCGC 3 cut(s) 22, 293, 570
AcsI RAATTY 1 cut(s) 110
AcuI CTGAAG 1 cut(s) 533
AdeI CACNNNGTG 1 cut(s) 567
AfaI GTAC 1 cut(s) 339
AfiI CCNNNNNNNGG 3 cut(s) 100, 210, 548
AgsI TTSAA 5 cut(s) 9, 273, 347, 472, 556
AluBI AGCT 4 cut(s) 436, 440, 479, 511
AluI AGCT 4 cut(s) 436, 440, 479, 511
Alw26I GTCTC 2 cut(s) 124, 395
AoxI GGCC 1 cut(s) 307
ApeKI GCWGC 2 cut(s) 24, 407
ApoI RAATTY 1 cut(s) 110
ArsI GACNNNNNNTTYG 2 cut(s) 102, 134
AspLEI GCGC 1 cut(s) 181
AsuHPI GGTGA 2 cut(s) 106, 181
AsuNHI GCTAGC 1 cut(s) 436
BaeGI GKGCMC 1 cut(s) 22
BanI GGYRCC 1 cut(s) 17
BanII GRGCYC 1 cut(s) 108
BbvI GCAGC 2 cut(s) 11, 394
BccI CCATC 2 cut(s) 56, 200
BcoDI GTCTC 2 cut(s) 124, 395
BfaI CTAG 3 cut(s) 65, 437, 492
BfmI CTRYAG 1 cut(s) 253
BfuAI ACCTGC 1 cut(s) 550
BisI GCNGC 4 cut(s) 25, 293, 408, 571
BlsI GCNGC 4 cut(s) 26, 294, 409, 572
BmiI GGNNCC 3 cut(s) 19, 105, 519
BmtI GCTAGC 1 cut(s) 440
BsaJI CCNNGG 2 cut(s) 99, 304
Bsc4I CCNNNNNNNGG 3 cut(s) 100, 210, 548
Bse118I RCCGGY 1 cut(s) 410
Bse1I ACTGG 1 cut(s) 553
Bse3DI GCAATG 2 cut(s) 243, 580
BseDI CCNNGG 2 cut(s) 99, 304
BseGI GGATG 4 cut(s) 48, 60, 192, 234
BseLI CCNNNNNNNGG 3 cut(s) 100, 210, 548
BseMI GCAATG 2 cut(s) 243, 580
BseMII CTCAG 1 cut(s) 401
BseNI ACTGG 1 cut(s) 553
BseSI GKGCMC 1 cut(s) 22
BseXI GCAGC 2 cut(s) 11, 394
BshFI GGCC 1 cut(s) 309
BshNI GGYRCC 1 cut(s) 17
BsiSI CCGG 1 cut(s) 411
BslI CCNNNNNNNGG 3 cut(s) 100, 210, 548
BsmAI GTCTC 2 cut(s) 124, 395
BsmBI CGTCTC 1 cut(s) 124
BsnI GGCC 1 cut(s) 309
Bsp1286I GDGCHC 2 cut(s) 22, 108
Bsp143I GATC 2 cut(s) 87, 498
BspACI CCGC 3 cut(s) 22, 293, 570
BspANI GGCC 1 cut(s) 309
BspCNI CTCAG 1 cut(s) 400
BspLI GGNNCC 3 cut(s) 19, 105, 519
BspMI ACCTGC 1 cut(s) 550
BspOI GCTAGC 1 cut(s) 440
BspT107I GGYRCC 1 cut(s) 17
BsrDI GCAATG 2 cut(s) 243, 580
BsrFI RCCGGY 1 cut(s) 410
BsrI ACTGG 1 cut(s) 553
BssAI RCCGGY 1 cut(s) 410
BssECI CCNNGG 2 cut(s) 99, 304
BssMI GATC 2 cut(s) 87, 498
BssT1I CCWWGG 2 cut(s) 99, 304
Bst4CI ACNGT 2 cut(s) 118, 230
Bst6I CTCTTC 1 cut(s) 35
BstC8I GCNNGC 4 cut(s) 22, 412, 438, 509
BstDEI CTNAG 1 cut(s) 387
BstENI CCTNNNNNAGG 1 cut(s) 98
BstF5I GGATG 4 cut(s) 48, 60, 192, 234
BstHHI GCGC 1 cut(s) 181
BstKTI GATC 2 cut(s) 90, 501
BstMAI GTCTC 2 cut(s) 124, 395
BstMBI GATC 2 cut(s) 87, 498
BstMWI GCNNNNNNNGC 1 cut(s) 33
BstSFI CTRYAG 1 cut(s) 253
BstSLI GKGCMC 1 cut(s) 22
BstV1I GCAGC 2 cut(s) 11, 394
BsuRI GGCC 1 cut(s) 309
BtsCI GGATG 4 cut(s) 48, 60, 192, 234
BtsIMutI CAGTG 1 cut(s) 131
BveI ACCTGC 1 cut(s) 550
Cac8I GCNNGC 4 cut(s) 22, 412, 438, 509
CfoI GCGC 1 cut(s) 181
Cfr10I RCCGGY 1 cut(s) 410
Csp6I GTAC 1 cut(s) 338
CviAII CATG 2 cut(s) 332, 377
CviJI RGCY 8 cut(s) 68, 106, 309, 436, 440, 455, 479, 511
CviKI_1 RGCY 8 cut(s) 68, 106, 309, 436, 440, 455, 479, 511
CviQI GTAC 1 cut(s) 338
DdeI CTNAG 1 cut(s) 387
DpnI GATC 2 cut(s) 89, 500
DpnII GATC 2 cut(s) 87, 498
DraIII CACNNNGTG 1 cut(s) 567
Eam1104I CTCTTC 1 cut(s) 35
EarI CTCTTC 1 cut(s) 35
Eco130I CCWWGG 2 cut(s) 99, 304
Eco24I GRGCYC 1 cut(s) 108
Eco57I CTGAAG 1 cut(s) 533
EcoNI CCTNNNNNAGG 1 cut(s) 98
EcoRI GAATTC 1 cut(s) 110
EcoT14I CCWWGG 2 cut(s) 99, 304
EcoT38I GRGCYC 1 cut(s) 108
ErhI CCWWGG 2 cut(s) 99, 304
Esp3I CGTCTC 1 cut(s) 124
FaeI CATG 2 cut(s) 335, 380
FaiI YATR 8 cut(s) 77, 221, 223, 333, 378, 462, 486, 582
FatI CATG 2 cut(s) 331, 376
FauI CCCGC 1 cut(s) 29
Fnu4HI GCNGC 4 cut(s) 25, 293, 408, 571
FokI GGATG 4 cut(s) 35, 47, 179, 221
FriOI GRGCYC 1 cut(s) 108
Fsp4HI GCNGC 4 cut(s) 25, 293, 408, 571
FspBI CTAG 3 cut(s) 65, 437, 492
GlaI GCGC 1 cut(s) 180
GluI GCNGC 4 cut(s) 25, 293, 408, 571
HaeIII GGCC 1 cut(s) 309
HapII CCGG 1 cut(s) 411
HhaI GCGC 1 cut(s) 181
Hin1II CATG 2 cut(s) 335, 380
Hin6I GCGC 1 cut(s) 179
HinP1I GCGC 1 cut(s) 179
HpaII CCGG 1 cut(s) 411
HphI GGTGA 2 cut(s) 106, 181
Hpy166II GTNNAC 1 cut(s) 418
Hpy188I TCNGA 1 cut(s) 400
Hpy188III TCNNGA 1 cut(s) 472
Hpy8I GTNNAC 1 cut(s) 418
HpyAV CCTTC 3 cut(s) 104, 341, 508
HpyCH4III ACNGT 2 cut(s) 118, 230
HpyCH4V TGCA 4 cut(s) 42, 331, 507, 545
HpyF10VI GCNNNNNNNGC 1 cut(s) 33
HpyF3I CTNAG 1 cut(s) 387
Hsp92II CATG 2 cut(s) 335, 380
HspAI GCGC 1 cut(s) 179
KroI GCCGGC 1 cut(s) 410
KroNI GCCGGC 1 cut(s) 412
Kzo9I GATC 2 cut(s) 87, 498
LmnI GCTCC 1 cut(s) 103
LpnPI CCDG 5 cut(s) 67, 338, 424, 534, 555
Lsp1109I GCAGC 2 cut(s) 11, 394
MaeI CTAG 3 cut(s) 65, 437, 492
MaeIII GTNAC 1 cut(s) 230
MalI GATC 2 cut(s) 89, 500
MboI GATC 2 cut(s) 87, 498
MboII GAAGA 2 cut(s) 22, 82
MhlI GDGCHC 2 cut(s) 22, 108
MluCI AATT 3 cut(s) 110, 140, 280
MnlI CCTC 6 cut(s) 38, 62, 68, 139, 258, 596
MroNI GCCGGC 1 cut(s) 410
MseI TTAA 2 cut(s) 279, 393
MspA1I CMGCKG 2 cut(s) 24, 479
MspI CCGG 1 cut(s) 411
MwoI GCNNNNNNNGC 1 cut(s) 33
NaeI GCCGGC 1 cut(s) 412
NdeII GATC 2 cut(s) 87, 498
NgoMIV GCCGGC 1 cut(s) 410
NheI GCTAGC 1 cut(s) 436
NlaIII CATG 2 cut(s) 335, 380
NlaIV GGNNCC 3 cut(s) 19, 105, 519
NmuCI GTSAC 1 cut(s) 230
PaqCI CACCTGC 1 cut(s) 550
PdiI GCCGGC 1 cut(s) 412
PkrI GCNGC 4 cut(s) 26, 294, 409, 572
PspN4I GGNNCC 3 cut(s) 19, 105, 519
PsrI GAACNNNNNNTAC 2 cut(s) 452, 484
PvuII CAGCTG 1 cut(s) 479
RsaI GTAC 1 cut(s) 339
RsaNI GTAC 1 cut(s) 338
SaqAI TTAA 2 cut(s) 279, 393
SatI GCNGC 4 cut(s) 25, 293, 408, 571
Sau3AI GATC 2 cut(s) 87, 498
SduI GDGCHC 2 cut(s) 22, 108
SfcI CTRYAG 1 cut(s) 253
Sse9I AATT 3 cut(s) 110, 140, 280
SsiI CCGC 3 cut(s) 22, 293, 570
SspI AATATT 1 cut(s) 424
SspMI CTAG 3 cut(s) 65, 437, 492
StyI CCWWGG 2 cut(s) 99, 304
TaaI ACNGT 2 cut(s) 118, 230
TaqI TCGA 1 cut(s) 501
TasI AATT 3 cut(s) 110, 140, 280
TatI WGTACW 1 cut(s) 337
TauI GCSGC 2 cut(s) 295, 573
Tru1I TTAA 2 cut(s) 279, 393
Tru9I TTAA 2 cut(s) 279, 393
TscAI CASTG 1 cut(s) 138
TseFI GTSAC 1 cut(s) 230
TseI GCWGC 2 cut(s) 24, 407
Tsp45I GTSAC 1 cut(s) 230
TspDTI ATGAA 2 cut(s) 64, 393
TspRI CASTG 1 cut(s) 138
XagI CCTNNNNNAGG 1 cut(s) 98
XapI RAATTY 1 cut(s) 110
XspI CTAG 3 cut(s) 65, 437, 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.