pycom14g02110

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0001039
Physical Location & Seq
Forward (+)
1655890 .. 1656495
606 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g02110.1

Sequence Viewer

Length: 606 bp
ATGACTCCAAAGTGCGGCACACATTGCCTCTACTTCGTTGGGCAGGCCTTCCTTTGCCTGTGCCTAACCACATTCATATTCTGGTTGATCTTCCTTCCCAAAGAGCCTAAATTCACCATCTCCAATGCCTCTCTAAGCCGATTCAACTTCACCAACACCGACAACACTCTCTTCTACAATCTCGCGCTCAATGTCACCATCCGAAACCCCAACAAAAGGGTTGGCATATACTACCGCCGCATCCAAGTCTTTGCCAACTACAGAAAAAAGAGGTTCGCTAATGTGACCTTGCCTTTGGAGCCATTTTACCAAGGCCGCAAGAACACGACCAATTTAAACCATGTACTCGTTGAAGGTCAGCAGTTGGTGGCATTTGGGGAACACGACCTTTCTCAGTTTAACTCGGAGACTGCTGCCGGTGTTTATAGTATTGATGTGGAGATTTATCTTCGGGTAAAAGCCAGGTACGGCAAGGTCAAGACATCAGATTACGGTTCTAGCAACATCGACTGCAAGCTGAAGGTTCCTGTGAGTTCTAACAACACATCCGTGAGTGGTTTCAATCCCACAAAGTGCGGGAATGTTCATGTCCTTCGCGAATCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

202

Amino Acids

22.88

Weight (kDa)

9.57

Isoelectric Point (pI)

29.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 65 - 168 1.4e-08 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 185, 597
AciI CCGC 5 cut(s) 15, 235, 238, 316, 576
AcsI RAATTY 1 cut(s) 110
AcuI CTGAAG 1 cut(s) 539
AdeI CACNNNGTG 1 cut(s) 573
AfaI GTAC 2 cut(s) 345, 467
AfiI CCNNNNNNNGG 2 cut(s) 14, 216
AgsI TTSAA 3 cut(s) 145, 353, 562
AjnI CCWGG 1 cut(s) 461
AleI CACNNNNGTG 1 cut(s) 548
AluBI AGCT 1 cut(s) 517
AluI AGCT 1 cut(s) 517
Alw26I GTCTC 1 cut(s) 401
AoxI GGCC 2 cut(s) 45, 313
ApeKI GCWGC 1 cut(s) 413
ApoI RAATTY 1 cut(s) 110
ArsI GACNNNNNNTTYG 2 cut(s) 277, 309
AspLEI GCGC 1 cut(s) 187
AsuHPI GGTGA 3 cut(s) 106, 142, 187
BbvI GCAGC 1 cut(s) 400
BccI CCATC 2 cut(s) 125, 206
BceAI ACGGC 1 cut(s) 484
BciT130I CCWGG 1 cut(s) 463
BcoDI GTCTC 1 cut(s) 401
BfaI CTAG 2 cut(s) 498, 604
BfmI CTRYAG 1 cut(s) 259
BisI GCNGC 4 cut(s) 16, 238, 316, 414
BlsI GCNGC 4 cut(s) 17, 239, 317, 415
Bme1390I CCNGG 1 cut(s) 463
BmiI GGNNCC 2 cut(s) 300, 525
BmrFI CCNGG 1 cut(s) 463
BmsI GCATC 1 cut(s) 249
BsaJI CCNNGG 1 cut(s) 310
Bsc4I CCNNNNNNNGG 2 cut(s) 14, 216
Bse118I RCCGGY 1 cut(s) 416
Bse3DI GCAATG 1 cut(s) 22
BseBI CCWGG 1 cut(s) 463
BseDI CCNNGG 1 cut(s) 310
BseGI GGATG 3 cut(s) 198, 240, 545
BseLI CCNNNNNNNGG 2 cut(s) 14, 216
BseMI GCAATG 1 cut(s) 22
BseMII CTCAG 1 cut(s) 407
BseXI GCAGC 1 cut(s) 400
Bsh1236I CGCG 2 cut(s) 185, 597
BshFI GGCC 2 cut(s) 47, 315
BsiSI CCGG 1 cut(s) 417
BslI CCNNNNNNNGG 2 cut(s) 14, 216
BsmAI GTCTC 1 cut(s) 401
BsnI GGCC 2 cut(s) 47, 315
Bsp143I GATC 1 cut(s) 87
Bsp68I TCGCGA 1 cut(s) 597
BspACI CCGC 5 cut(s) 15, 235, 238, 316, 576
BspANI GGCC 2 cut(s) 47, 315
BspCNI CTCAG 1 cut(s) 406
BspFNI CGCG 2 cut(s) 185, 597
BspLI GGNNCC 2 cut(s) 300, 525
BsrDI GCAATG 1 cut(s) 22
BsrFI RCCGGY 1 cut(s) 416
BssAI RCCGGY 1 cut(s) 416
BssECI CCNNGG 1 cut(s) 310
BssMI GATC 1 cut(s) 87
BssT1I CCWWGG 1 cut(s) 310
Bst2UI CCWGG 1 cut(s) 463
Bst4CI ACNGT 1 cut(s) 494
Bst6I CTCTTC 1 cut(s) 176
BstAPI GCANNNNNTGC 1 cut(s) 24
BstC8I GCNNGC 2 cut(s) 45, 515
BstDEI CTNAG 2 cut(s) 134, 393
BstF5I GGATG 3 cut(s) 198, 240, 545
BstFNI CGCG 2 cut(s) 185, 597
BstHHI GCGC 1 cut(s) 187
BstKTI GATC 1 cut(s) 90
BstMAI GTCTC 1 cut(s) 401
BstMBI GATC 1 cut(s) 87
BstMWI GCNNNNNNNGC 2 cut(s) 24, 298
BstNI CCWGG 1 cut(s) 463
BstSCI CCNGG 1 cut(s) 461
BstSFI CTRYAG 1 cut(s) 259
BstUI CGCG 2 cut(s) 185, 597
BstV1I GCAGC 1 cut(s) 400
BsuRI GGCC 2 cut(s) 47, 315
BtsCI GGATG 3 cut(s) 198, 240, 545
BtuMI TCGCGA 1 cut(s) 597
Cac8I GCNNGC 2 cut(s) 45, 515
CfoI GCGC 1 cut(s) 187
Cfr10I RCCGGY 1 cut(s) 416
Csp6I GTAC 2 cut(s) 344, 466
CviAII CATG 2 cut(s) 341, 587
CviJI RGCY 7 cut(s) 47, 106, 138, 301, 315, 461, 517
CviKI_1 RGCY 7 cut(s) 47, 106, 138, 301, 315, 461, 517
CviQI GTAC 2 cut(s) 344, 466
DdeI CTNAG 2 cut(s) 134, 393
DpnI GATC 1 cut(s) 89
DpnII GATC 1 cut(s) 87
DraI TTTAAA 1 cut(s) 336
DraIII CACNNNGTG 1 cut(s) 573
Eam1104I CTCTTC 1 cut(s) 176
EarI CTCTTC 1 cut(s) 176
Eco130I CCWWGG 1 cut(s) 310
Eco147I AGGCCT 1 cut(s) 47
Eco57I CTGAAG 1 cut(s) 539
EcoRII CCWGG 1 cut(s) 461
EcoT14I CCWWGG 1 cut(s) 310
ErhI CCWWGG 1 cut(s) 310
FaeI CATG 2 cut(s) 344, 590
FaiI YATR 6 cut(s) 77, 227, 229, 342, 426, 588
FatI CATG 2 cut(s) 340, 586
FauI CCCGC 1 cut(s) 569
Fnu4HI GCNGC 4 cut(s) 16, 238, 316, 414
FokI GGATG 3 cut(s) 185, 227, 532
Fsp4HI GCNGC 4 cut(s) 16, 238, 316, 414
FspBI CTAG 2 cut(s) 498, 604
GlaI GCGC 1 cut(s) 186
GluI GCNGC 4 cut(s) 16, 238, 316, 414
HaeIII GGCC 2 cut(s) 47, 315
HapII CCGG 1 cut(s) 417
HhaI GCGC 1 cut(s) 187
Hin1II CATG 2 cut(s) 344, 590
Hin6I GCGC 1 cut(s) 185
HinP1I GCGC 1 cut(s) 185
HinfI GANTC 3 cut(s) 4, 141, 599
HpaII CCGG 1 cut(s) 417
HphI GGTGA 3 cut(s) 106, 142, 187
Hpy188I TCNGA 3 cut(s) 203, 406, 487
Hpy188III TCNNGA 2 cut(s) 478, 596
HpyAV CCTTC 5 cut(s) 58, 104, 347, 514, 602
HpyCH4III ACNGT 1 cut(s) 494
HpyCH4V TGCA 1 cut(s) 513
HpyF10VI GCNNNNNNNGC 2 cut(s) 24, 298
HpyF3I CTNAG 2 cut(s) 134, 393
Hsp92II CATG 2 cut(s) 344, 590
HspAI GCGC 1 cut(s) 185
Kzo9I GATC 1 cut(s) 87
LmnI GCTCC 1 cut(s) 298
LpnPI CCDG 7 cut(s) 29, 67, 71, 430, 448, 475, 540
Lsp1109I GCAGC 1 cut(s) 400
LweI GCATC 1 cut(s) 249
MaeI CTAG 2 cut(s) 498, 604
MaeIII GTNAC 2 cut(s) 193, 283
MalI GATC 1 cut(s) 89
MboI GATC 1 cut(s) 87
MboII GAAGA 3 cut(s) 82, 163, 440
MluCI AATT 2 cut(s) 110, 331
MnlI CCTC 3 cut(s) 38, 139, 264
MseI TTAA 2 cut(s) 335, 399
MslI CAYNNNNRTG 1 cut(s) 548
MspI CCGG 1 cut(s) 417
MspR9I CCNGG 1 cut(s) 463
MvaI CCWGG 1 cut(s) 463
MvnI CGCG 2 cut(s) 185, 597
MwoI GCNNNNNNNGC 2 cut(s) 24, 298
NdeII GATC 1 cut(s) 87
NlaIII CATG 2 cut(s) 344, 590
NlaIV GGNNCC 2 cut(s) 300, 525
NmuCI GTSAC 2 cut(s) 193, 283
NruI TCGCGA 1 cut(s) 597
OliI CACNNNNGTG 1 cut(s) 548
PceI AGGCCT 1 cut(s) 47
PfeI GAWTC 2 cut(s) 141, 599
PkrI GCNGC 4 cut(s) 17, 239, 317, 415
Psp6I CCWGG 1 cut(s) 461
PspGI CCWGG 1 cut(s) 461
PspN4I GGNNCC 2 cut(s) 300, 525
RruI TCGCGA 1 cut(s) 597
RsaI GTAC 2 cut(s) 345, 467
RsaNI GTAC 2 cut(s) 344, 466
RseI CAYNNNNRTG 1 cut(s) 548
SaqAI TTAA 2 cut(s) 335, 399
SatI GCNGC 4 cut(s) 16, 238, 316, 414
Sau3AI GATC 1 cut(s) 87
ScrFI CCNGG 1 cut(s) 463
SetI ASST 8 cut(s) 275, 290, 358, 390, 467, 477, 519, 525
SfaNI GCATC 1 cut(s) 249
SfcI CTRYAG 1 cut(s) 259
SmiMI CAYNNNNRTG 1 cut(s) 548
Sse9I AATT 2 cut(s) 110, 331
SseBI AGGCCT 1 cut(s) 47
SsiI CCGC 5 cut(s) 15, 235, 238, 316, 576
SspMI CTAG 2 cut(s) 498, 604
StuI AGGCCT 1 cut(s) 47
StyD4I CCNGG 1 cut(s) 461
StyI CCWWGG 1 cut(s) 310
TaaI ACNGT 1 cut(s) 494
TaqI TCGA 1 cut(s) 507
TasI AATT 2 cut(s) 110, 331
TatI WGTACW 1 cut(s) 343
TauI GCSGC 3 cut(s) 18, 240, 318
TfiI GAWTC 2 cut(s) 141, 599
Tru1I TTAA 2 cut(s) 335, 399
Tru9I TTAA 2 cut(s) 335, 399
TseFI GTSAC 2 cut(s) 193, 283
TseI GCWGC 1 cut(s) 413
Tsp45I GTSAC 2 cut(s) 193, 283
TspDTI ATGAA 2 cut(s) 64, 575
TspGWI ACGGA 1 cut(s) 538
XapI RAATTY 1 cut(s) 110
XspI CTAG 2 cut(s) 498, 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.