pycom12g02310

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001039
Physical Location & Seq
Forward (+)
2017902 .. 2018453
552 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g02310.1

Sequence Viewer

Length: 483 bp
ATGTGCATATTCTGGTTGATCTTCCTTCCCAAAGAGCCTCAATTCACCGTCTCCAATGCCTCTCTAACCCGATTCAATTTCACCGACAGTGACAATACTCTCGACTACGATCTCGCACTCAACATCACCATCCGAAACCCCAACAAAAGGGTTGGCACATACCGCCACATCCAAGTCTTTGCCAACTATAGGAAAAAGAGGTTTGCTATTGTGACGCTGCCTTGGGCGCCCTTTTACCAAGGGCATAAGAACACGACCATTTTAAATCATGTACTCGTTGAAGGTCATATTGATGTGAAGATTTCTCTTCAGGTAAGAGCCAGGTTCGGAAAGATCAAGACACCAGATTATGGTCCTAGCAACATCAACTGCAACCTGAAGGTTCCCTTGAGTTTAAGCGAAACATTGCCAAGTGGTTTCAATGCCACAAAGTGCAGGAATGTTCATGTCCTCACAAATCCAAGCGACTACGCATGGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

161

Amino Acids

18.38

Weight (kDa)

9.71

Isoelectric Point (pI)

40.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 226
AccB7I CCANNNNNTGG 1 cut(s) 350
AciI CCGC 1 cut(s) 163
AcuI CTGAAG 2 cut(s) 293, 398
AcyI GRCGYC 1 cut(s) 227
AdeI CACNNNGTG 1 cut(s) 432
AfaI GTAC 1 cut(s) 273
AfiI CCNNNNNNNGG 3 cut(s) 147, 189, 350
AgsI TTSAA 3 cut(s) 76, 281, 421
AjnI CCWGG 1 cut(s) 320
Alw26I GTCTC 1 cut(s) 55
ApeKI GCWGC 1 cut(s) 217
AspLEI GCGC 1 cut(s) 229
AspS9I GGNCC 1 cut(s) 353
AsuHPI GGTGA 3 cut(s) 37, 73, 118
AvaII GGWCC 1 cut(s) 353
BanI GGYRCC 1 cut(s) 226
BbvI GCAGC 1 cut(s) 204
BccI CCATC 1 cut(s) 137
BciT130I CCWGG 1 cut(s) 322
BcoDI GTCTC 1 cut(s) 55
BfaI CTAG 1 cut(s) 357
BfmI CTRYAG 1 cut(s) 187
BfoI RGCGCY 1 cut(s) 230
BisI GCNGC 1 cut(s) 218
BlsI GCNGC 1 cut(s) 219
Bme1390I CCNGG 1 cut(s) 322
Bme18I GGWCC 1 cut(s) 353
BmgT120I GGNCC 1 cut(s) 353
BmiI GGNNCC 2 cut(s) 228, 384
BmrFI CCNGG 1 cut(s) 322
BpuEI CTTGAG 1 cut(s) 409
BsaHI GRCGYC 1 cut(s) 227
BsaJI CCNNGG 2 cut(s) 221, 238
Bsc4I CCNNNNNNNGG 3 cut(s) 147, 189, 350
Bse3DI GCAATG 1 cut(s) 404
BseBI CCWGG 1 cut(s) 322
BseDI CCNNGG 2 cut(s) 221, 238
BseGI GGATG 2 cut(s) 129, 168
BseLI CCNNNNNNNGG 3 cut(s) 147, 189, 350
BseMI GCAATG 1 cut(s) 404
BseXI GCAGC 1 cut(s) 204
BsgI GTGCAG 1 cut(s) 454
BshNI GGYRCC 1 cut(s) 226
BslI CCNNNNNNNGG 3 cut(s) 147, 189, 350
BsmAI GTCTC 1 cut(s) 55
BsmBI CGTCTC 1 cut(s) 55
Bsp143I GATC 3 cut(s) 18, 109, 333
BspACI CCGC 1 cut(s) 163
BspLI GGNNCC 2 cut(s) 228, 384
BspT107I GGYRCC 1 cut(s) 226
BsrDI GCAATG 1 cut(s) 404
BssECI CCNNGG 2 cut(s) 221, 238
BssMI GATC 3 cut(s) 18, 109, 333
BssNI GRCGYC 1 cut(s) 227
BssT1I CCWWGG 2 cut(s) 221, 238
Bst2UI CCWGG 1 cut(s) 322
Bst4CI ACNGT 2 cut(s) 49, 89
Bst6I CTCTTC 1 cut(s) 312
BstACI GRCGYC 1 cut(s) 227
BstF5I GGATG 2 cut(s) 129, 168
BstH2I RGCGCY 1 cut(s) 230
BstHHI GCGC 1 cut(s) 229
BstKTI GATC 3 cut(s) 21, 112, 336
BstMAI GTCTC 1 cut(s) 55
BstMBI GATC 3 cut(s) 18, 109, 333
BstMWI GCNNNNNNNGC 2 cut(s) 162, 226
BstNI CCWGG 1 cut(s) 322
BstSCI CCNGG 1 cut(s) 320
BstSFI CTRYAG 1 cut(s) 187
BstV1I GCAGC 1 cut(s) 204
BtsCI GGATG 2 cut(s) 129, 168
BtsIMutI CAGTG 1 cut(s) 94
CfoI GCGC 1 cut(s) 229
Cfr13I GGNCC 1 cut(s) 353
CseI GACGC 1 cut(s) 223
Csp6I GTAC 1 cut(s) 272
CviAII CATG 3 cut(s) 269, 446, 474
CviJI RGCY 2 cut(s) 37, 320
CviKI_1 RGCY 2 cut(s) 37, 320
CviQI GTAC 1 cut(s) 272
DinI GGCGCC 1 cut(s) 228
DpnI GATC 3 cut(s) 20, 111, 335
DpnII GATC 3 cut(s) 18, 109, 333
DraI TTTAAA 1 cut(s) 264
DraIII CACNNNGTG 1 cut(s) 432
Eam1104I CTCTTC 1 cut(s) 312
EarI CTCTTC 1 cut(s) 312
Eco130I CCWWGG 2 cut(s) 221, 238
Eco47I GGWCC 1 cut(s) 353
Eco57I CTGAAG 2 cut(s) 293, 398
EcoRII CCWGG 1 cut(s) 320
EcoT14I CCWWGG 2 cut(s) 221, 238
EgeI GGCGCC 1 cut(s) 228
EheI GGCGCC 1 cut(s) 228
ErhI CCWWGG 2 cut(s) 221, 238
Esp3I CGTCTC 1 cut(s) 55
FaeI CATG 3 cut(s) 272, 449, 477
FaiI YATR 9 cut(s) 8, 160, 189, 246, 270, 288, 351, 447, 475
FalI AAGNNNNNCTT 2 cut(s) 371, 403
FatI CATG 3 cut(s) 268, 445, 473
Fnu4HI GCNGC 1 cut(s) 218
FokI GGATG 2 cut(s) 116, 155
Fsp4HI GCNGC 1 cut(s) 218
FspBI CTAG 1 cut(s) 357
GlaI GCGC 1 cut(s) 228
GluI GCNGC 1 cut(s) 218
HaeII RGCGCY 1 cut(s) 230
HgaI GACGC 1 cut(s) 223
HhaI GCGC 1 cut(s) 229
Hin1I GRCGYC 1 cut(s) 227
Hin1II CATG 3 cut(s) 272, 449, 477
Hin6I GCGC 1 cut(s) 227
HinP1I GCGC 1 cut(s) 227
HinfI GANTC 1 cut(s) 72
HphI GGTGA 3 cut(s) 37, 73, 118
Hpy188I TCNGA 2 cut(s) 134, 329
Hpy188III TCNNGA 2 cut(s) 101, 337
HpyAV CCTTC 3 cut(s) 35, 275, 373
HpyCH4III ACNGT 2 cut(s) 49, 89
HpyCH4V TGCA 3 cut(s) 6, 372, 435
HpyF10VI GCNNNNNNNGC 2 cut(s) 162, 226
Hsp92I GRCGYC 1 cut(s) 227
Hsp92II CATG 3 cut(s) 272, 449, 477
HspAI GCGC 1 cut(s) 227
KasI GGCGCC 1 cut(s) 226
Kzo9I GATC 3 cut(s) 18, 109, 333
LpnPI CCDG 6 cut(s) 296, 307, 334, 357, 389, 421
Lsp1109I GCAGC 1 cut(s) 204
MaeI CTAG 1 cut(s) 357
MaeIII GTNAC 2 cut(s) 89, 211
MalI GATC 3 cut(s) 20, 111, 335
MboI GATC 3 cut(s) 18, 109, 333
MboII GAAGA 3 cut(s) 13, 299, 310
MluCI AATT 2 cut(s) 41, 76
Mly113I GGCGCC 1 cut(s) 227
MnlI CCTC 4 cut(s) 48, 70, 192, 461
MseI TTAA 2 cut(s) 263, 395
MslI CAYNNNNRTG 1 cut(s) 291
MspR9I CCNGG 1 cut(s) 322
MvaI CCWGG 1 cut(s) 322
MwoI GCNNNNNNNGC 2 cut(s) 162, 226
NarI GGCGCC 1 cut(s) 227
NdeII GATC 3 cut(s) 18, 109, 333
NlaIII CATG 3 cut(s) 272, 449, 477
NlaIV GGNNCC 2 cut(s) 228, 384
NmuCI GTSAC 2 cut(s) 89, 211
PfeI GAWTC 1 cut(s) 72
PflMI CCANNNNNTGG 1 cut(s) 350
PkrI GCNGC 1 cut(s) 219
PluTI GGCGCC 1 cut(s) 230
Psp6I CCWGG 1 cut(s) 320
PspGI CCWGG 1 cut(s) 320
PspN4I GGNNCC 2 cut(s) 228, 384
PspPI GGNCC 1 cut(s) 353
RsaI GTAC 1 cut(s) 273
RsaNI GTAC 1 cut(s) 272
RseI CAYNNNNRTG 1 cut(s) 291
SaqAI TTAA 2 cut(s) 263, 395
SatI GCNGC 1 cut(s) 218
Sau3AI GATC 3 cut(s) 18, 109, 333
Sau96I GGNCC 1 cut(s) 353
ScrFI CCNGG 1 cut(s) 322
SetI ASST 6 cut(s) 203, 286, 315, 326, 378, 384
SfcI CTRYAG 1 cut(s) 187
SfoI GGCGCC 1 cut(s) 228
SinI GGWCC 1 cut(s) 353
SmiMI CAYNNNNRTG 1 cut(s) 291
SmlI CTYRAG 1 cut(s) 388
SmoI CTYRAG 1 cut(s) 388
Sse9I AATT 2 cut(s) 41, 76
SsiI CCGC 1 cut(s) 163
SspDI GGCGCC 1 cut(s) 226
SspMI CTAG 1 cut(s) 357
StyD4I CCNGG 1 cut(s) 320
StyI CCWWGG 2 cut(s) 221, 238
TaaI ACNGT 2 cut(s) 49, 89
TaqI TCGA 1 cut(s) 102
TasI AATT 2 cut(s) 41, 76
TatI WGTACW 1 cut(s) 271
TfiI GAWTC 1 cut(s) 72
Tru1I TTAA 2 cut(s) 263, 395
Tru9I TTAA 2 cut(s) 263, 395
TscAI CASTG 1 cut(s) 94
TseFI GTSAC 2 cut(s) 89, 211
TseI GCWGC 1 cut(s) 217
Tsp45I GTSAC 2 cut(s) 89, 211
TspDTI ATGAA 1 cut(s) 434
TspRI CASTG 1 cut(s) 94
Van91I CCANNNNNTGG 1 cut(s) 350
VpaK11BI GGWCC 1 cut(s) 353
XspI CTAG 1 cut(s) 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.