Prupe.7G112000_v2.0.a1

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0001039
Physical Location & Seq
Reverse (-)
13855618 .. 13856316
699 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G112000.1

Sequence Viewer

Length: 699 bp
ATGCGTAATGGAAGGCAAAGCCGCTGGTGCAACTTCTCTTGCTTCTTCTGGATCTTCTACTACTCCATCCTCGCCTTCATTCTTTTCATTGTCGCCATCATCATCTTCTGGTTGATCTTTCAACCTCAGGAGCTCAAATTCACCGTCACCGATGCCTCGTTGACCCAATTCAATCTCACCAACACCAGCATCACCAACACCACCAACAACACTCTATACTACAACCTCGCCCTCAACGTATCCATAAGAAATCCTAACAAAAAGGTCGGTGTATATTACAATCGGATCCAAGCCATTGGCAACTATGGGAAAAAGAGGTTTGCATTGGTCAACACCACTTCCACTCCATTTTACCAGGGCCACAAGAACACAACCATGGTGCGTTTTGTGCTTCAAGGGCAGCAAGTGGTGGTGCTTGGGGGAAAGGAGCTTTCTCGGTTTAACTCGGAAACAAGTGCTAGGGTTTACAATATTGATGTGAAGCTGGCTCAACGGATAAAGGCTAGGTATGGTAAGATCAAGACTGCCTACTTCAAGCCCCCAAAGGTGGATTGTCAACTCAAACTTCCTTTGAGTACTATTTATAACAATGGAACTTCTGTAGGTCCTAAGTTTGTTTCCAAGGAGTGTGGGAGTGTCAAAATCTTTACAATCTCCGTGGGGATTGGGATTGGTCCTTTTAGTTTTGAAACAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

233

Amino Acids

26.4

Weight (kDa)

10.04

Isoelectric Point (pI)

23.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 585
AciI CCGC 1 cut(s) 22
AclWI GGATC 3 cut(s) 59, 280, 293
AcsI RAATTY 1 cut(s) 137
AfaI GTAC 1 cut(s) 577
AfiI CCNNNNNNNGG 1 cut(s) 547
AgsI TTSAA 5 cut(s) 122, 172, 395, 535, 689
AjnI CCWGG 1 cut(s) 354
AluBI AGCT 3 cut(s) 133, 430, 484
AluI AGCT 3 cut(s) 133, 430, 484
Alw21I GWGCWC 1 cut(s) 135
AlwI GGATC 3 cut(s) 59, 280, 293
AoxI GGCC 1 cut(s) 358
ApeKI GCWGC 1 cut(s) 400
ApoI RAATTY 1 cut(s) 137
ArsI GACNNNNNNTTYG 2 cut(s) 129, 161
AspS9I GGNCC 3 cut(s) 358, 605, 674
AsuHPI GGTGA 4 cut(s) 133, 139, 169, 184
AvaII GGWCC 2 cut(s) 605, 674
AxyI CCTNAGG 1 cut(s) 126
BamHI GGATCC 1 cut(s) 285
BanII GRGCYC 1 cut(s) 135
Bbv12I GWGCWC 1 cut(s) 135
BbvI GCAGC 1 cut(s) 412
BccI CCATC 2 cut(s) 74, 104
BciT130I CCWGG 1 cut(s) 356
BciVI GTATCC 1 cut(s) 250
BfaI CTAG 2 cut(s) 459, 504
BfmI CTRYAG 1 cut(s) 600
BfuI GTATCC 1 cut(s) 250
BisI GCNGC 2 cut(s) 22, 401
BlsI GCNGC 2 cut(s) 23, 402
BmcAI AGTACT 1 cut(s) 577
Bme1390I CCNGG 1 cut(s) 356
Bme18I GGWCC 2 cut(s) 605, 674
BmgT120I GGNCC 3 cut(s) 358, 605, 674
BmiI GGNNCC 1 cut(s) 287
BmrFI CCNGG 1 cut(s) 356
BmsI GCATC 2 cut(s) 142, 198
BsaJI CCNNGG 4 cut(s) 355, 375, 621, 657
BsaXI ACNNNNNCTCC 2 cut(s) 328, 358
Bsc4I CCNNNNNNNGG 1 cut(s) 547
Bse21I CCTNAGG 1 cut(s) 126
BseBI CCWGG 1 cut(s) 356
BseDI CCNNGG 4 cut(s) 355, 375, 621, 657
BseGI GGATG 1 cut(s) 66
BseLI CCNNNNNNNGG 1 cut(s) 547
BseMII CTCAG 1 cut(s) 140
BseXI GCAGC 1 cut(s) 412
BshFI GGCC 1 cut(s) 360
BsiHKAI GWGCWC 1 cut(s) 135
BslI CCNNNNNNNGG 1 cut(s) 547
BsnI GGCC 1 cut(s) 360
Bsp1286I GDGCHC 1 cut(s) 135
Bsp143I GATC 4 cut(s) 51, 114, 285, 516
Bsp19I CCATGG 1 cut(s) 375
BspACI CCGC 1 cut(s) 22
BspANI GGCC 1 cut(s) 360
BspCNI CTCAG 1 cut(s) 139
BspLI GGNNCC 1 cut(s) 287
BspPI GGATC 3 cut(s) 59, 280, 293
BssECI CCNNGG 4 cut(s) 355, 375, 621, 657
BssMI GATC 4 cut(s) 51, 114, 285, 516
BssT1I CCWWGG 2 cut(s) 375, 621
Bst2UI CCWGG 1 cut(s) 356
Bst4CI ACNGT 1 cut(s) 145
BstC8I GCNNGC 1 cut(s) 486
BstDEI CTNAG 2 cut(s) 126, 609
BstDSI CCRYGG 2 cut(s) 375, 657
BstF5I GGATG 1 cut(s) 66
BstKTI GATC 4 cut(s) 54, 117, 288, 519
BstMBI GATC 4 cut(s) 51, 114, 285, 516
BstMWI GCNNNNNNNGC 3 cut(s) 27, 388, 397
BstNI CCWGG 1 cut(s) 356
BstSCI CCNGG 1 cut(s) 354
BstSFI CTRYAG 1 cut(s) 600
BstV1I GCAGC 1 cut(s) 412
BstX2I RGATCY 2 cut(s) 51, 285
BstXI CCANNNNNNTGG 1 cut(s) 296
BstYI RGATCY 2 cut(s) 51, 285
Bsu36I CCTNAGG 1 cut(s) 126
BsuI GTATCC 1 cut(s) 250
BsuRI GGCC 1 cut(s) 360
BtgI CCRYGG 2 cut(s) 375, 657
BtsCI GGATG 1 cut(s) 66
Cac8I GCNNGC 1 cut(s) 486
Cfr13I GGNCC 3 cut(s) 358, 605, 674
Csp6I GTAC 1 cut(s) 576
CspCI CAANNNNNGTGG 4 cut(s) 610, 639, 645, 674
CviAII CATG 1 cut(s) 376
CviQI GTAC 1 cut(s) 576
DdeI CTNAG 2 cut(s) 126, 609
DpnI GATC 4 cut(s) 53, 116, 287, 518
DpnII GATC 4 cut(s) 51, 114, 285, 516
Ecl136II GAGCTC 1 cut(s) 133
Eco130I CCWWGG 2 cut(s) 375, 621
Eco24I GRGCYC 1 cut(s) 135
Eco47I GGWCC 2 cut(s) 605, 674
Eco53kI GAGCTC 1 cut(s) 133
Eco81I CCTNAGG 1 cut(s) 126
EcoICRI GAGCTC 1 cut(s) 133
EcoO109I RGGNCCY 1 cut(s) 605
EcoRII CCWGG 1 cut(s) 354
EcoT14I CCWWGG 2 cut(s) 375, 621
EcoT38I GRGCYC 1 cut(s) 135
ErhI CCWWGG 2 cut(s) 375, 621
FaeI CATG 1 cut(s) 379
FaiI YATR 7 cut(s) 217, 245, 274, 306, 377, 510, 585
FatI CATG 1 cut(s) 375
Fnu4HI GCNGC 2 cut(s) 22, 401
FokI GGATG 1 cut(s) 53
FriOI GRGCYC 1 cut(s) 135
Fsp4HI GCNGC 2 cut(s) 22, 401
FspBI CTAG 2 cut(s) 459, 504
GluI GCNGC 2 cut(s) 22, 401
HaeIII GGCC 1 cut(s) 360
Hin1II CATG 1 cut(s) 379
HincII GTYRAC 3 cut(s) 162, 331, 557
HindII GTYRAC 3 cut(s) 162, 331, 557
HphI GGTGA 4 cut(s) 133, 139, 169, 184
Hpy166II GTNNAC 4 cut(s) 162, 331, 466, 557
Hpy188I TCNGA 2 cut(s) 285, 448
Hpy188III TCNNGA 3 cut(s) 49, 128, 520
Hpy8I GTNNAC 4 cut(s) 162, 331, 466, 557
HpyAV CCTTC 2 cut(s) 6, 85
HpyCH4III ACNGT 1 cut(s) 145
HpyCH4IV ACGT 1 cut(s) 237
HpyCH4V TGCA 2 cut(s) 30, 323
HpyF10VI GCNNNNNNNGC 3 cut(s) 27, 388, 397
HpyF3I CTNAG 2 cut(s) 126, 609
HpySE526I ACGT 1 cut(s) 237
Hsp92II CATG 1 cut(s) 379
Kzo9I GATC 4 cut(s) 51, 114, 285, 516
LmnI GCTCC 2 cut(s) 130, 427
LpnPI CCDG 9 cut(s) 10, 34, 94, 113, 199, 341, 368, 470, 678
Lsp1109I GCAGC 1 cut(s) 412
LweI GCATC 2 cut(s) 142, 198
MaeI CTAG 2 cut(s) 459, 504
MaeII ACGT 1 cut(s) 237
MaeIII GTNAC 1 cut(s) 145
MalI GATC 4 cut(s) 53, 116, 287, 518
MboI GATC 4 cut(s) 51, 114, 285, 516
MboII GAAGA 3 cut(s) 37, 46, 97
MflI RGATCY 2 cut(s) 51, 285
MhlI GDGCHC 1 cut(s) 135
MluCI AATT 2 cut(s) 137, 167
MnlI CCTC 6 cut(s) 80, 135, 166, 236, 242, 309
MseI TTAA 1 cut(s) 441
MslI CAYNNNNRTG 1 cut(s) 374
MspA1I CMGCKG 1 cut(s) 24
MspR9I CCNGG 1 cut(s) 356
MvaI CCWGG 1 cut(s) 356
MwoI GCNNNNNNNGC 3 cut(s) 27, 388, 397
NcoI CCATGG 1 cut(s) 375
NdeII GATC 4 cut(s) 51, 114, 285, 516
NlaIII CATG 1 cut(s) 379
NlaIV GGNNCC 1 cut(s) 287
NmuCI GTSAC 1 cut(s) 145
PcsI WCGNNNNNNNCGW 1 cut(s) 234
PkrI GCNGC 2 cut(s) 23, 402
PpuMI RGGWCCY 1 cut(s) 605
PsiI TTATAA 1 cut(s) 585
Psp124BI GAGCTC 1 cut(s) 135
Psp5II RGGWCCY 1 cut(s) 605
Psp6I CCWGG 1 cut(s) 354
PspGI CCWGG 1 cut(s) 354
PspN4I GGNNCC 1 cut(s) 287
PspPI GGNCC 3 cut(s) 358, 605, 674
PspPPI RGGWCCY 1 cut(s) 605
PsuI RGATCY 2 cut(s) 51, 285
RsaI GTAC 1 cut(s) 577
RsaNI GTAC 1 cut(s) 576
RseI CAYNNNNRTG 1 cut(s) 374
SacI GAGCTC 1 cut(s) 135
SaqAI TTAA 1 cut(s) 441
SatI GCNGC 2 cut(s) 22, 401
Sau3AI GATC 4 cut(s) 51, 114, 285, 516
Sau96I GGNCC 3 cut(s) 358, 605, 674
ScaI AGTACT 1 cut(s) 577
ScrFI CCNGG 1 cut(s) 356
SduI GDGCHC 1 cut(s) 135
SfaNI GCATC 2 cut(s) 142, 198
SfcI CTRYAG 1 cut(s) 600
SinI GGWCC 2 cut(s) 605, 674
SmiMI CAYNNNNRTG 1 cut(s) 374
Sse9I AATT 2 cut(s) 137, 167
SsiI CCGC 1 cut(s) 22
SspI AATATT 1 cut(s) 472
SspMI CTAG 2 cut(s) 459, 504
SstI GAGCTC 1 cut(s) 135
StyD4I CCNGG 1 cut(s) 354
StyI CCWWGG 2 cut(s) 375, 621
TaaI ACNGT 1 cut(s) 145
TaiI ACGT 1 cut(s) 240
TasI AATT 2 cut(s) 137, 167
TatI WGTACW 1 cut(s) 575
TauI GCSGC 1 cut(s) 24
Tru1I TTAA 1 cut(s) 441
Tru9I TTAA 1 cut(s) 441
TseFI GTSAC 1 cut(s) 145
TseI GCWGC 1 cut(s) 400
Tsp45I GTSAC 1 cut(s) 145
TspDTI ATGAA 2 cut(s) 67, 76
TspGWI ACGGA 2 cut(s) 508, 646
VpaK11BI GGWCC 2 cut(s) 605, 674
XapI RAATTY 1 cut(s) 137
XspI CTAG 2 cut(s) 459, 504
ZrmI AGTACT 1 cut(s) 577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.