Rw0G008080

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00301
OG0001039
Physical Location & Seq
Forward (+)
13104 .. 14967
1864 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G008080.1

Sequence Viewer

Length: 558 bp
ATGCGTATGATAAAATGCAATCACAGCTGCTGCAAACTCTGCTTCTATCTCATCTTTCCATACCCAAGAACCCAAATTTACTTACCATCACAGTTTAATCCACTGCGAACCAACAAAACTCTCCACTACAACCTTGAGCTCAACATTACCATCACAAACCCCAATAAGAAGGTCGACATATACTACAATGGCATCCAAGTCATTGCTAACTATAAAACAAAGGAGTTTGCCATGCTGACTTTAGATTCCACACCATTTTACCAAGGCCACAAGAACACAACCATTTTGGATCATGTGGTACTTGAAGGGCAGAAATCGGTGGAGTTCAAGGAGCCAGAGGTTTCCCAATTTAAGGCAGAGACTGCTGCTGGTTTTTACAGTATTGATGTGCAGCTTGCTCTTCGAGTAAAGCTCGGGTATGACATGTTCAAGACAAGGTATTACCAGCAGTCAGGCAACAAGATCGATTGCAAGCTGAAGGTTCCTCTGAGACATGCAAGACGTTTCAAAACTACAAAGTGTGGAAATGTATACATTATTTCAGATCCTGCTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

185

Amino Acids

21.6

Weight (kDa)

9.43

Isoelectric Point (pI)

37.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 49 - 147 2.7e-08 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 174, 531
AclWI GGATC 2 cut(s) 297, 539
AcsI RAATTY 1 cut(s) 75
AcuI CTGAAG 1 cut(s) 497
AfaI GTAC 1 cut(s) 300
AfiI CCNNNNNNNGG 1 cut(s) 352
AflIII ACRYGT 1 cut(s) 423
AgsI TTSAA 4 cut(s) 305, 328, 430, 508
AluBI AGCT 5 cut(s) 27, 139, 394, 412, 475
AluI AGCT 5 cut(s) 27, 139, 394, 412, 475
Alw21I GWGCWC 1 cut(s) 141
Alw26I GTCTC 2 cut(s) 353, 484
AlwI GGATC 2 cut(s) 297, 539
AlwNI CAGNNNCTG 3 cut(s) 30, 362, 548
Ama87I CYCGRG 1 cut(s) 413
AoxI GGCC 1 cut(s) 265
ApeKI GCWGC 4 cut(s) 27, 30, 365, 391
ApoI RAATTY 1 cut(s) 75
AvaI CYCGRG 1 cut(s) 413
BanII GRGCYC 1 cut(s) 141
Bbv12I GWGCWC 1 cut(s) 141
BbvI GCAGC 4 cut(s) 14, 17, 352, 403
BccI CCATC 2 cut(s) 94, 158
BcgI CGANNNNNNTGC 2 cut(s) 445, 479
BcoDI GTCTC 2 cut(s) 353, 484
BisI GCNGC 4 cut(s) 28, 31, 366, 392
BlsI GCNGC 4 cut(s) 29, 32, 367, 393
BmeT110I CYCGRG 1 cut(s) 413
BmiI GGNNCC 2 cut(s) 333, 483
BmsI GCATC 1 cut(s) 201
BplI GAGNNNNNCTC 2 cut(s) 396, 428
BpuEI CTTGAG 1 cut(s) 155
Bsa29I ATCGAT 1 cut(s) 465
BsaJI CCNNGG 1 cut(s) 262
Bsc4I CCNNNNNNNGG 1 cut(s) 352
Bse3DI GCAATG 1 cut(s) 201
BseCI ATCGAT 1 cut(s) 465
BseDI CCNNGG 1 cut(s) 262
BseGI GGATG 1 cut(s) 192
BseLI CCNNNNNNNGG 1 cut(s) 352
BseMI GCAATG 1 cut(s) 201
BseMII CTCAG 1 cut(s) 479
BseXI GCAGC 4 cut(s) 14, 17, 352, 403
BsgI GTGCAG 1 cut(s) 410
BshFI GGCC 1 cut(s) 267
BshVI ATCGAT 1 cut(s) 465
BsiHKAI GWGCWC 1 cut(s) 141
BsiHKCI CYCGRG 1 cut(s) 413
BslI CCNNNNNNNGG 1 cut(s) 352
BsmAI GTCTC 2 cut(s) 353, 484
BsnI GGCC 1 cut(s) 267
BsoBI CYCGRG 1 cut(s) 413
Bsp1286I GDGCHC 1 cut(s) 141
Bsp143I GATC 3 cut(s) 289, 462, 544
BspANI GGCC 1 cut(s) 267
BspCNI CTCAG 1 cut(s) 480
BspDI ATCGAT 1 cut(s) 465
BspLI GGNNCC 2 cut(s) 333, 483
BspPI GGATC 2 cut(s) 297, 539
BspQI GCTCTTC 1 cut(s) 405
BsrDI GCAATG 1 cut(s) 201
BssECI CCNNGG 1 cut(s) 262
BssMI GATC 3 cut(s) 289, 462, 544
BssNAI GTATAC 1 cut(s) 532
BssT1I CCWWGG 1 cut(s) 262
Bst1107I GTATAC 1 cut(s) 532
Bst4CI ACNGT 2 cut(s) 93, 380
Bst6I CTCTTC 1 cut(s) 405
BstAPI GCANNNNNTGC 2 cut(s) 39, 362
BstC8I GCNNGC 2 cut(s) 396, 473
BstDEI CTNAG 1 cut(s) 488
BstF5I GGATG 1 cut(s) 192
BstKTI GATC 3 cut(s) 292, 465, 547
BstMAI GTCTC 2 cut(s) 353, 484
BstMBI GATC 3 cut(s) 289, 462, 544
BstMWI GCNNNNNNNGC 3 cut(s) 24, 39, 362
BstNSI RCATGY 2 cut(s) 427, 497
BstV1I GCAGC 4 cut(s) 14, 17, 352, 403
BstX2I RGATCY 1 cut(s) 544
BstYI RGATCY 1 cut(s) 544
BstZ17I GTATAC 1 cut(s) 532
Bsu15I ATCGAT 1 cut(s) 465
BsuRI GGCC 1 cut(s) 267
BsuTUI ATCGAT 1 cut(s) 465
BtsCI GGATG 1 cut(s) 192
BtsI GCAGTG 1 cut(s) 101
BtsIMutI CAGTG 1 cut(s) 101
Cac8I GCNNGC 2 cut(s) 396, 473
CaiI CAGNNNCTG 3 cut(s) 30, 362, 548
ClaI ATCGAT 1 cut(s) 465
Csp6I GTAC 1 cut(s) 299
CviAII CATG 4 cut(s) 232, 293, 424, 494
CviJI RGCY 7 cut(s) 27, 139, 267, 334, 394, 412, 475
CviKI_1 RGCY 7 cut(s) 27, 139, 267, 334, 394, 412, 475
CviQI GTAC 1 cut(s) 299
DdeI CTNAG 1 cut(s) 488
DpnI GATC 3 cut(s) 291, 464, 546
DpnII GATC 3 cut(s) 289, 462, 544
Eam1104I CTCTTC 1 cut(s) 405
EarI CTCTTC 1 cut(s) 405
Ecl136II GAGCTC 1 cut(s) 139
Eco130I CCWWGG 1 cut(s) 262
Eco24I GRGCYC 1 cut(s) 141
Eco53kI GAGCTC 1 cut(s) 139
Eco57I CTGAAG 1 cut(s) 497
Eco88I CYCGRG 1 cut(s) 413
EcoICRI GAGCTC 1 cut(s) 139
EcoT14I CCWWGG 1 cut(s) 262
EcoT38I GRGCYC 1 cut(s) 141
ErhI CCWWGG 1 cut(s) 262
FaeI CATG 4 cut(s) 235, 296, 427, 497
FatI CATG 4 cut(s) 231, 292, 423, 493
FblI GTMKAC 2 cut(s) 174, 531
Fnu4HI GCNGC 4 cut(s) 28, 31, 366, 392
FokI GGATG 1 cut(s) 179
FriOI GRGCYC 1 cut(s) 141
Fsp4HI GCNGC 4 cut(s) 28, 31, 366, 392
GluI GCNGC 4 cut(s) 28, 31, 366, 392
HaeIII GGCC 1 cut(s) 267
Hin1II CATG 4 cut(s) 235, 296, 427, 497
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HinfI GANTC 1 cut(s) 245
Hpy166II GTNNAC 2 cut(s) 175, 532
Hpy188I TCNGA 2 cut(s) 489, 544
Hpy188III TCNNGA 1 cut(s) 430
Hpy8I GTNNAC 2 cut(s) 175, 532
HpyAV CCTTC 3 cut(s) 163, 299, 472
HpyCH4III ACNGT 2 cut(s) 93, 380
HpyCH4IV ACGT 1 cut(s) 502
HpyCH4V TGCA 5 cut(s) 18, 33, 391, 471, 497
HpyF10VI GCNNNNNNNGC 3 cut(s) 24, 39, 362
HpyF3I CTNAG 1 cut(s) 488
HpySE526I ACGT 1 cut(s) 502
Hsp92II CATG 4 cut(s) 235, 296, 427, 497
Kzo9I GATC 3 cut(s) 289, 462, 544
LguI GCTCTTC 1 cut(s) 405
LmnI GCTCC 1 cut(s) 331
LpnPI CCDG 4 cut(s) 348, 354, 438, 458
Lsp1109I GCAGC 4 cut(s) 14, 17, 352, 403
LweI GCATC 1 cut(s) 201
MaeII ACGT 1 cut(s) 502
MalI GATC 3 cut(s) 291, 464, 546
MboI GATC 3 cut(s) 289, 462, 544
MboII GAAGA 1 cut(s) 392
MflI RGATCY 1 cut(s) 544
MhlI GDGCHC 1 cut(s) 141
MluCI AATT 2 cut(s) 75, 347
MnlI CCTC 2 cut(s) 331, 495
MseI TTAA 2 cut(s) 96, 351
MspA1I CMGCKG 1 cut(s) 27
MwoI GCNNNNNNNGC 3 cut(s) 24, 39, 362
NdeII GATC 3 cut(s) 289, 462, 544
NlaIII CATG 4 cut(s) 235, 296, 427, 497
NlaIV GGNNCC 2 cut(s) 333, 483
NspI RCATGY 2 cut(s) 427, 497
PciI ACATGT 1 cut(s) 423
PciSI GCTCTTC 1 cut(s) 405
PfeI GAWTC 1 cut(s) 245
PkrI GCNGC 4 cut(s) 29, 32, 367, 393
PscI ACATGT 1 cut(s) 423
Psp124BI GAGCTC 1 cut(s) 141
PspN4I GGNNCC 2 cut(s) 333, 483
PsrI GAACNNNNNNTAC 2 cut(s) 410, 442
PstNI CAGNNNCTG 3 cut(s) 30, 362, 548
PsuI RGATCY 1 cut(s) 544
PvuII CAGCTG 1 cut(s) 27
RsaI GTAC 1 cut(s) 300
RsaNI GTAC 1 cut(s) 299
SacI GAGCTC 1 cut(s) 141
SalI GTCGAC 1 cut(s) 173
SapI GCTCTTC 1 cut(s) 405
SaqAI TTAA 2 cut(s) 96, 351
SatI GCNGC 4 cut(s) 28, 31, 366, 392
Sau3AI GATC 3 cut(s) 289, 462, 544
SduI GDGCHC 1 cut(s) 141
SfaNI GCATC 1 cut(s) 201
SmlI CTYRAG 1 cut(s) 134
SmoI CTYRAG 1 cut(s) 134
Sse9I AATT 2 cut(s) 75, 347
SstI GAGCTC 1 cut(s) 141
StyI CCWWGG 1 cut(s) 262
TaaI ACNGT 2 cut(s) 93, 380
TaiI ACGT 1 cut(s) 505
TaqI TCGA 3 cut(s) 174, 403, 465
TasI AATT 2 cut(s) 75, 347
TfiI GAWTC 1 cut(s) 245
Tru1I TTAA 2 cut(s) 96, 351
Tru9I TTAA 2 cut(s) 96, 351
TscAI CASTG 1 cut(s) 108
TseI GCWGC 4 cut(s) 27, 30, 365, 391
TspRI CASTG 1 cut(s) 108
XapI RAATTY 1 cut(s) 75
XceI RCATGY 2 cut(s) 427, 497
XmiI GTMKAC 2 cut(s) 174, 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.