MD12G1035400.v1.1

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001039
Physical Location & Seq
Forward (+)
3864892 .. 3868090
3199 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1035400.v1.1.491

Sequence Viewer

Length: 306 bp
ATGTACTCGTTGAAGGTCAGCAGTTGGCGGTATTTGGAGAACGTGACCTCTCTCAGTTTTAACTCGGAGACTGCTGCTGGTGTTTACAGTATTGATGTGAAGATTTCTCTTCGGGTAAGAGCCAGGTTCGGAAAGATCAAGACACCAGATTATGGTCCTAGCAACATCAACTGCAACCTGAAGGTTCCTTTGAGTTTAAGCGAAACATTGCCGAGTGGTTTCAATGCCACAAAGTGCAGGAATGTTCATGTCCTCACAAATCCAAGCAACTGGCCACGGATTTCAGATTTTCGGTCCAAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

102

Amino Acids

11.37

Weight (kDa)

10.02

Isoelectric Point (pI)

23.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 152
AciI CCGC 1 cut(s) 28
AcoI YGGCCR 1 cut(s) 272
AcuI CTGAAG 1 cut(s) 200
AdeI CACNNNGTG 1 cut(s) 234
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 1 cut(s) 152
AgsI TTSAA 2 cut(s) 13, 223
AjnI CCWGG 1 cut(s) 122
Alw26I GTCTC 1 cut(s) 62
AoxI GGCC 1 cut(s) 272
ApeKI GCWGC 1 cut(s) 74
AspS9I GGNCC 2 cut(s) 155, 294
AvaII GGWCC 2 cut(s) 155, 294
BalI TGGCCA 1 cut(s) 274
BbvI GCAGC 1 cut(s) 61
BciT130I CCWGG 1 cut(s) 124
BcoDI GTCTC 1 cut(s) 62
BfaI CTAG 1 cut(s) 159
BisI GCNGC 1 cut(s) 75
BlsI GCNGC 1 cut(s) 76
Bme1390I CCNGG 1 cut(s) 124
Bme18I GGWCC 2 cut(s) 155, 294
BmgT120I GGNCC 2 cut(s) 155, 294
BmiI GGNNCC 1 cut(s) 186
BmrFI CCNGG 1 cut(s) 124
BsaJI CCNNGG 1 cut(s) 275
Bsc4I CCNNNNNNNGG 1 cut(s) 152
Bse1I ACTGG 1 cut(s) 275
Bse3DI GCAATG 1 cut(s) 206
BseBI CCWGG 1 cut(s) 124
BseDI CCNNGG 1 cut(s) 275
BseLI CCNNNNNNNGG 1 cut(s) 152
BseMI GCAATG 1 cut(s) 206
BseMII CTCAG 1 cut(s) 67
BseNI ACTGG 1 cut(s) 275
BseXI GCAGC 1 cut(s) 61
BsgI GTGCAG 1 cut(s) 256
BshFI GGCC 1 cut(s) 274
BslI CCNNNNNNNGG 1 cut(s) 152
BsmAI GTCTC 1 cut(s) 62
BsnI GGCC 1 cut(s) 274
Bsp143I GATC 1 cut(s) 135
BspACI CCGC 1 cut(s) 28
BspANI GGCC 1 cut(s) 274
BspCNI CTCAG 1 cut(s) 66
BspLI GGNNCC 1 cut(s) 186
BsrDI GCAATG 1 cut(s) 206
BsrI ACTGG 1 cut(s) 275
BssECI CCNNGG 1 cut(s) 275
BssMI GATC 1 cut(s) 135
Bst2UI CCWGG 1 cut(s) 124
Bst4CI ACNGT 1 cut(s) 89
Bst6I CTCTTC 1 cut(s) 114
BstDEI CTNAG 1 cut(s) 53
BstDSI CCRYGG 1 cut(s) 275
BstKTI GATC 1 cut(s) 138
BstMAI GTCTC 1 cut(s) 62
BstMBI GATC 1 cut(s) 135
BstNI CCWGG 1 cut(s) 124
BstSCI CCNGG 1 cut(s) 122
BstV1I GCAGC 1 cut(s) 61
BstXI CCANNNNNNTGG 1 cut(s) 270
BsuRI GGCC 1 cut(s) 274
BtgI CCRYGG 1 cut(s) 275
Cfr13I GGNCC 2 cut(s) 155, 294
Csp6I GTAC 1 cut(s) 4
CviAII CATG 1 cut(s) 248
CviJI RGCY 2 cut(s) 122, 274
CviKI_1 RGCY 2 cut(s) 122, 274
CviQI GTAC 1 cut(s) 4
DdeI CTNAG 1 cut(s) 53
DpnI GATC 1 cut(s) 137
DpnII GATC 1 cut(s) 135
DraIII CACNNNGTG 1 cut(s) 234
EaeI YGGCCR 1 cut(s) 272
Eam1104I CTCTTC 1 cut(s) 114
EarI CTCTTC 1 cut(s) 114
Eco47I GGWCC 2 cut(s) 155, 294
Eco57I CTGAAG 1 cut(s) 200
EcoRII CCWGG 1 cut(s) 122
FaeI CATG 1 cut(s) 251
FaiI YATR 2 cut(s) 153, 249
FatI CATG 1 cut(s) 247
Fnu4HI GCNGC 1 cut(s) 75
Fsp4HI GCNGC 1 cut(s) 75
FspBI CTAG 1 cut(s) 159
GluI GCNGC 1 cut(s) 75
HaeIII GGCC 1 cut(s) 274
Hin1II CATG 1 cut(s) 251
Hpy166II GTNNAC 1 cut(s) 85
Hpy188I TCNGA 3 cut(s) 67, 131, 286
Hpy188III TCNNGA 1 cut(s) 139
Hpy8I GTNNAC 1 cut(s) 85
HpyAV CCTTC 2 cut(s) 7, 175
HpyCH4III ACNGT 1 cut(s) 89
HpyCH4IV ACGT 1 cut(s) 42
HpyCH4V TGCA 2 cut(s) 174, 237
HpyF3I CTNAG 1 cut(s) 53
HpySE526I ACGT 1 cut(s) 42
Hsp92II CATG 1 cut(s) 251
Kzo9I GATC 1 cut(s) 135
LpnPI CCDG 7 cut(s) 63, 109, 136, 159, 191, 223, 256
Lsp1109I GCAGC 1 cut(s) 61
MaeI CTAG 1 cut(s) 159
MaeII ACGT 1 cut(s) 42
MaeIII GTNAC 1 cut(s) 43
MalI GATC 1 cut(s) 137
MboI GATC 1 cut(s) 135
MboII GAAGA 2 cut(s) 101, 112
MlsI TGGCCA 1 cut(s) 274
MluNI TGGCCA 1 cut(s) 274
MnlI CCTC 2 cut(s) 58, 263
Mox20I TGGCCA 1 cut(s) 274
MscI TGGCCA 1 cut(s) 274
MseI TTAA 2 cut(s) 60, 197
Msp20I TGGCCA 1 cut(s) 274
MspR9I CCNGG 1 cut(s) 124
MvaI CCWGG 1 cut(s) 124
NdeII GATC 1 cut(s) 135
NlaIII CATG 1 cut(s) 251
NlaIV GGNNCC 1 cut(s) 186
NmeAIII GCCGAG 1 cut(s) 237
NmuCI GTSAC 1 cut(s) 43
PflMI CCANNNNNTGG 1 cut(s) 152
PkrI GCNGC 1 cut(s) 76
Psp6I CCWGG 1 cut(s) 122
PspGI CCWGG 1 cut(s) 122
PspN4I GGNNCC 1 cut(s) 186
PspPI GGNCC 2 cut(s) 155, 294
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
SaqAI TTAA 2 cut(s) 60, 197
SatI GCNGC 1 cut(s) 75
Sau3AI GATC 1 cut(s) 135
Sau96I GGNCC 2 cut(s) 155, 294
ScrFI CCNGG 1 cut(s) 124
SetI ASST 6 cut(s) 18, 45, 50, 128, 180, 186
SinI GGWCC 2 cut(s) 155, 294
SsiI CCGC 1 cut(s) 28
SspMI CTAG 1 cut(s) 159
StyD4I CCNGG 1 cut(s) 122
TaaI ACNGT 1 cut(s) 89
TaiI ACGT 1 cut(s) 45
TaqII GACCGA 1 cut(s) 282
TatI WGTACW 1 cut(s) 3
Tru1I TTAA 2 cut(s) 60, 197
Tru9I TTAA 2 cut(s) 60, 197
TseFI GTSAC 1 cut(s) 43
TseI GCWGC 1 cut(s) 74
Tsp45I GTSAC 1 cut(s) 43
TspDTI ATGAA 1 cut(s) 236
TspGWI ACGGA 1 cut(s) 292
Van91I CCANNNNNTGG 1 cut(s) 152
VpaK11BI GGWCC 2 cut(s) 155, 294
XspI CTAG 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.