Rroxscaffold_6G00408320

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001039
Physical Location & Seq
Reverse (-)
30759189 .. 30760890
1702 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00408320.1

Sequence Viewer

Length: 861 bp
ATGTCTAGAAGGTGTGGTTGCCGCTTGAACTGCTGCCCCTGCTGCTTCTGGATATACTACTGCATCATCCTCTGCTTCAGCCTACCCTTCGTGATATTCGGGTTCATCTTCTTTCCCGAAGAGCCCAAATTCACAATCACCAATGCCACTCTAATCCAATTCAATTTTAACAGTTCCAACAACACCCTCGATTACAACCTCGCACTCAACATGACCATCAGAAACCCTAACAAGAGGGTTGGCATATATTACCGTCGCATCCAAGTCATTGGTAACTACCGAAAGAAGAGATTTGCTCTAGTCACTTTGACTACTTTGCCGTTCTACCAAGGCCATAAAAACACCACCGATTTGCATCAACTGGTTGTTCAAGGGTCGCAACTGGTGAAGTTTGGAAAACGCGAGGTGTCCCAGTTTAACTCGGAGACTGCTTCCGGGATTTATAGAATAAACGTGCAGCTAGCTCTTCGGATAAAAGCCAGGTACGGCAAGTTCAAGTCGCGCAATTACAAGCCGTATAGGAAGATTGACTGCAAGCTGAAGGTTCCTTTGAGCTTTAATGAAACGTCAACGGCCATTGGTTTTAAGGCTACAAGACTGGCAGCGGGGTTTGTGGGCTGTTCAAGACCAGTAGCGTTGGATTCTGAATCAACGGTGGTGCAGATGTTTTGCGCACTGGACCGGCGTGATTTTGGATCGGGACTTAACGGCCTGAGCTGTGGCGGTTGGGGTCGTGGCGGCGTGGGGTGCGGAATGGATCGGGCAGAGGAGCAGCAAACCAACGAACGACATGACGTCAAGGAGATGTTGGGCCTGGACCAAGCCAGCTTGATGGGCTCTGAGTTGGAACCTTCCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

286

Amino Acids

32.37

Weight (kDa)

9.47

Isoelectric Point (pI)

36.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 71 - 164 1.5e-07 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 798
Acc16I TGCGCA 1 cut(s) 673
AccII CGCG 2 cut(s) 402, 502
AciI CCGC 5 cut(s) 22, 605, 723, 738, 750
AclWI GGATC 2 cut(s) 703, 765
AcoI YGGCCR 1 cut(s) 573
AcsI RAATTY 1 cut(s) 128
AcuI CTGAAG 2 cut(s) 61, 560
AcyI GRCGYC 1 cut(s) 795
AfaI GTAC 1 cut(s) 485
AgsI TTSAA 5 cut(s) 28, 163, 371, 496, 624
AhdI GACNNNNNGTC 1 cut(s) 794
AjnI CCWGG 2 cut(s) 479, 813
AluBI AGCT 6 cut(s) 460, 464, 538, 555, 717, 828
AluI AGCT 6 cut(s) 460, 464, 538, 555, 717, 828
Alw26I GTCTC 1 cut(s) 419
AlwI GGATC 2 cut(s) 703, 765
AoxI GGCC 4 cut(s) 331, 573, 709, 811
ApeKI GCWGC 5 cut(s) 33, 42, 457, 602, 772
ApoI RAATTY 1 cut(s) 128
AspLEI GCGC 2 cut(s) 504, 674
AspS9I GGNCC 3 cut(s) 679, 811, 817
AsuC2I CCSGG 1 cut(s) 436
AsuHPI GGTGA 2 cut(s) 130, 397
AsuNHI GCTAGC 1 cut(s) 460
AvaII GGWCC 2 cut(s) 679, 817
BanII GRGCYC 2 cut(s) 126, 839
BbvI GCAGC 5 cut(s) 20, 29, 469, 614, 784
BccI CCATC 2 cut(s) 224, 826
BceAI ACGGC 5 cut(s) 304, 499, 502, 588, 724
BciT130I CCWGG 2 cut(s) 481, 815
BcnI CCSGG 1 cut(s) 436
BcoDI GTCTC 1 cut(s) 419
BfaI CTAG 3 cut(s) 6, 299, 461
BisI GCNGC 7 cut(s) 22, 34, 43, 458, 603, 739, 773
BlsI GCNGC 7 cut(s) 23, 35, 44, 459, 604, 740, 774
Bme1390I CCNGG 3 cut(s) 436, 481, 815
Bme18I GGWCC 2 cut(s) 679, 817
BmeRI GACNNNNNGTC 1 cut(s) 794
BmgT120I GGNCC 3 cut(s) 679, 811, 817
BmiI GGNNCC 2 cut(s) 546, 849
BmrFI CCNGG 3 cut(s) 436, 481, 815
BmrI ACTGGG 1 cut(s) 406
BmsI GCATC 3 cut(s) 72, 267, 364
BmtI GCTAGC 1 cut(s) 464
BmuI ACTGGG 1 cut(s) 406
BplI GAGNNNNNCTC 2 cut(s) 280, 312
Bpu10I CCTNAGC 1 cut(s) 713
BpuMI CCSGG 1 cut(s) 436
BsaBI GATNNNNATC 1 cut(s) 354
BsaHI GRCGYC 1 cut(s) 795
BsaJI CCNNGG 1 cut(s) 328
Bse118I RCCGGY 1 cut(s) 681
Bse1I ACTGG 6 cut(s) 366, 387, 412, 603, 629, 681
Bse8I GATNNNNATC 1 cut(s) 354
BseBI CCWGG 2 cut(s) 481, 815
BseDI CCNNGG 1 cut(s) 328
BseGI GGATG 2 cut(s) 66, 258
BseJI GATNNNNATC 1 cut(s) 354
BseMII CTCAG 2 cut(s) 704, 831
BseNI ACTGG 6 cut(s) 366, 387, 412, 603, 629, 681
BseRI GAGGAG 1 cut(s) 782
BseXI GCAGC 5 cut(s) 20, 29, 469, 614, 784
BsgI GTGCAG 2 cut(s) 476, 680
Bsh1236I CGCG 2 cut(s) 402, 502
BshFI GGCC 4 cut(s) 333, 575, 711, 813
BsiSI CCGG 2 cut(s) 435, 682
BslFI GGGAC 2 cut(s) 394, 714
BsmAI GTCTC 1 cut(s) 419
BsmFI GGGAC 2 cut(s) 394, 714
BsnI GGCC 4 cut(s) 333, 575, 711, 813
Bsp1286I GDGCHC 2 cut(s) 126, 839
Bsp143I GATC 2 cut(s) 695, 757
BspACI CCGC 5 cut(s) 22, 605, 723, 738, 750
BspANI GGCC 4 cut(s) 333, 575, 711, 813
BspCNI CTCAG 2 cut(s) 705, 832
BspFNI CGCG 2 cut(s) 402, 502
BspLI GGNNCC 2 cut(s) 546, 849
BspOI GCTAGC 1 cut(s) 464
BspPI GGATC 2 cut(s) 703, 765
BspQI GCTCTTC 2 cut(s) 114, 471
BsrFI RCCGGY 1 cut(s) 681
BsrI ACTGG 6 cut(s) 366, 387, 412, 603, 629, 681
BssAI RCCGGY 1 cut(s) 681
BssECI CCNNGG 1 cut(s) 328
BssMI GATC 2 cut(s) 695, 757
BssNI GRCGYC 1 cut(s) 795
BssT1I CCWWGG 1 cut(s) 328
Bst2UI CCWGG 2 cut(s) 481, 815
Bst4CI ACNGT 3 cut(s) 173, 254, 655
Bst6I CTCTTC 3 cut(s) 114, 281, 471
BstACI GRCGYC 1 cut(s) 795
BstC8I GCNNGC 3 cut(s) 462, 536, 826
BstDEI CTNAG 2 cut(s) 713, 840
BstF5I GGATG 2 cut(s) 66, 258
BstFNI CGCG 2 cut(s) 402, 502
BstHHI GCGC 2 cut(s) 504, 674
BstKTI GATC 2 cut(s) 698, 760
BstMAI GTCTC 1 cut(s) 419
BstMBI GATC 2 cut(s) 695, 757
BstMWI GCNNNNNNNGC 5 cut(s) 30, 39, 42, 747, 834
BstNI CCWGG 2 cut(s) 481, 815
BstSCI CCNGG 3 cut(s) 434, 479, 813
BstUI CGCG 2 cut(s) 402, 502
BstV1I GCAGC 5 cut(s) 20, 29, 469, 614, 784
BstXI CCANNNNNNTGG 2 cut(s) 269, 832
BsuRI GGCC 4 cut(s) 333, 575, 711, 813
BtsCI GGATG 2 cut(s) 66, 258
BtsIMutI CAGTG 1 cut(s) 674
Cac8I GCNNGC 3 cut(s) 462, 536, 826
CfoI GCGC 2 cut(s) 504, 674
Cfr10I RCCGGY 1 cut(s) 681
Cfr13I GGNCC 3 cut(s) 679, 811, 817
Csp6I GTAC 1 cut(s) 484
CviAII CATG 2 cut(s) 211, 791
CviQI GTAC 1 cut(s) 484
DdeI CTNAG 2 cut(s) 713, 840
DpnI GATC 2 cut(s) 697, 759
DpnII GATC 2 cut(s) 695, 757
DriI GACNNNNNGTC 1 cut(s) 794
EaeI YGGCCR 1 cut(s) 573
Eam1104I CTCTTC 3 cut(s) 114, 281, 471
Eam1105I GACNNNNNGTC 1 cut(s) 794
EarI CTCTTC 3 cut(s) 114, 281, 471
Eco130I CCWWGG 1 cut(s) 328
Eco24I GRGCYC 2 cut(s) 126, 839
Eco47I GGWCC 2 cut(s) 679, 817
Eco57I CTGAAG 2 cut(s) 61, 560
EcoRII CCWGG 2 cut(s) 479, 813
EcoT14I CCWWGG 1 cut(s) 328
EcoT38I GRGCYC 2 cut(s) 126, 839
ErhI CCWWGG 1 cut(s) 328
FaeI CATG 2 cut(s) 214, 794
FaiI YATR 8 cut(s) 55, 212, 245, 247, 336, 444, 519, 792
FaqI GGGAC 2 cut(s) 394, 714
FatI CATG 2 cut(s) 210, 790
FauI CCCGC 1 cut(s) 598
Fnu4HI GCNGC 7 cut(s) 22, 34, 43, 458, 603, 739, 773
FokI GGATG 2 cut(s) 53, 245
FriOI GRGCYC 2 cut(s) 126, 839
Fsp4HI GCNGC 7 cut(s) 22, 34, 43, 458, 603, 739, 773
FspBI CTAG 3 cut(s) 6, 299, 461
FspI TGCGCA 1 cut(s) 673
GlaI GCGC 2 cut(s) 503, 673
GluI GCNGC 7 cut(s) 22, 34, 43, 458, 603, 739, 773
HaeIII GGCC 4 cut(s) 333, 575, 711, 813
HapII CCGG 2 cut(s) 435, 682
HhaI GCGC 2 cut(s) 504, 674
Hin1I GRCGYC 1 cut(s) 795
Hin1II CATG 2 cut(s) 214, 794
Hin6I GCGC 2 cut(s) 502, 672
HinP1I GCGC 2 cut(s) 502, 672
HincII GTYRAC 1 cut(s) 570
HindII GTYRAC 1 cut(s) 570
HinfI GANTC 2 cut(s) 641, 647
HpaII CCGG 2 cut(s) 435, 682
HphI GGTGA 2 cut(s) 130, 397
Hpy166II GTNNAC 1 cut(s) 570
Hpy188I TCNGA 5 cut(s) 221, 424, 471, 646, 841
Hpy188III TCNNGA 7 cut(s) 6, 49, 91, 116, 624, 699, 858
Hpy8I GTNNAC 1 cut(s) 570
Hpy99I CGWCG 1 cut(s) 258
HpyAV CCTTC 4 cut(s) 3, 97, 535, 861
HpyCH4III ACNGT 3 cut(s) 173, 254, 655
HpyCH4IV ACGT 3 cut(s) 453, 566, 795
HpyCH4V TGCA 5 cut(s) 63, 355, 457, 534, 661
HpyF10VI GCNNNNNNNGC 5 cut(s) 30, 39, 42, 747, 834
HpyF3I CTNAG 2 cut(s) 713, 840
HpySE526I ACGT 3 cut(s) 453, 566, 795
Hsp92I GRCGYC 1 cut(s) 795
Hsp92II CATG 2 cut(s) 214, 794
HspAI GCGC 2 cut(s) 502, 672
Kzo9I GATC 2 cut(s) 695, 757
LguI GCTCTTC 2 cut(s) 114, 471
LmnI GCTCC 1 cut(s) 769
Lsp1109I GCAGC 5 cut(s) 20, 29, 469, 614, 784
LweI GCATC 3 cut(s) 72, 267, 364
MaeI CTAG 3 cut(s) 6, 299, 461
MaeII ACGT 3 cut(s) 453, 566, 795
MaeIII GTNAC 2 cut(s) 272, 301
MalI GATC 2 cut(s) 697, 759
MboI GATC 2 cut(s) 695, 757
MboII GAAGA 5 cut(s) 100, 131, 298, 458, 535
MhlI GDGCHC 2 cut(s) 126, 839
MluCI AATT 4 cut(s) 128, 158, 163, 505
MmeI TCCRAC 3 cut(s) 201, 618, 825
MnlI CCTC 6 cut(s) 80, 197, 209, 228, 397, 760
MseI TTAA 5 cut(s) 168, 417, 558, 585, 705
MspA1I CMGCKG 1 cut(s) 605
MspI CCGG 2 cut(s) 435, 682
MspR9I CCNGG 3 cut(s) 436, 481, 815
MvaI CCWGG 2 cut(s) 481, 815
MvnI CGCG 2 cut(s) 402, 502
MwoI GCNNNNNNNGC 5 cut(s) 30, 39, 42, 747, 834
NciI CCSGG 1 cut(s) 436
NdeII GATC 2 cut(s) 695, 757
NheI GCTAGC 1 cut(s) 460
NlaIII CATG 2 cut(s) 214, 794
NlaIV GGNNCC 2 cut(s) 546, 849
NmuCI GTSAC 1 cut(s) 301
NsbI TGCGCA 1 cut(s) 673
PciSI GCTCTTC 2 cut(s) 114, 471
PfeI GAWTC 2 cut(s) 641, 647
PfoI TCCNGGA 1 cut(s) 434
PkrI GCNGC 7 cut(s) 23, 35, 44, 459, 604, 740, 774
Psp6I CCWGG 2 cut(s) 479, 813
PspGI CCWGG 2 cut(s) 479, 813
PspN4I GGNNCC 2 cut(s) 546, 849
PspPI GGNCC 3 cut(s) 679, 811, 817
PsrI GAACNNNNNNTAC 2 cut(s) 476, 508
RsaI GTAC 1 cut(s) 485
RsaNI GTAC 1 cut(s) 484
SapI GCTCTTC 2 cut(s) 114, 471
SaqAI TTAA 5 cut(s) 168, 417, 558, 585, 705
SatI GCNGC 7 cut(s) 22, 34, 43, 458, 603, 739, 773
Sau3AI GATC 2 cut(s) 695, 757
Sau96I GGNCC 3 cut(s) 679, 811, 817
ScrFI CCNGG 3 cut(s) 436, 481, 815
SduI GDGCHC 2 cut(s) 126, 839
SfaNI GCATC 3 cut(s) 72, 267, 364
SinI GGWCC 2 cut(s) 679, 817
Sse9I AATT 4 cut(s) 128, 158, 163, 505
SsiI CCGC 5 cut(s) 22, 605, 723, 738, 750
SspMI CTAG 3 cut(s) 6, 299, 461
StyD4I CCNGG 3 cut(s) 434, 479, 813
StyI CCWWGG 1 cut(s) 328
TaaI ACNGT 3 cut(s) 173, 254, 655
TaiI ACGT 3 cut(s) 456, 569, 798
TaqI TCGA 1 cut(s) 189
TasI AATT 4 cut(s) 128, 158, 163, 505
TauI GCSGC 2 cut(s) 24, 741
TfiI GAWTC 2 cut(s) 641, 647
Tru1I TTAA 5 cut(s) 168, 417, 558, 585, 705
Tru9I TTAA 5 cut(s) 168, 417, 558, 585, 705
TscAI CASTG 1 cut(s) 681
TseFI GTSAC 1 cut(s) 301
TseI GCWGC 5 cut(s) 33, 42, 457, 602, 772
Tsp45I GTSAC 1 cut(s) 301
TspDTI ATGAA 2 cut(s) 94, 576
TspRI CASTG 1 cut(s) 681
VpaK11BI GGWCC 2 cut(s) 679, 817
XapI RAATTY 1 cut(s) 128
XbaI TCTAGA 1 cut(s) 5
XspI CTAG 3 cut(s) 6, 299, 461
ZraI GACGTC 1 cut(s) 796
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.