Rw0G014690

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00657
OG0001039
Physical Location & Seq
Forward (+)
11009 .. 11566
558 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G014690.1

Sequence Viewer

Length: 558 bp
ATGACATGTAACGGAAAGAAACTAACAAGCTACACCATTCTCTCTATATTTACCGCCATCGCCGTCTTAGCCGGAGTCATAGCATTCGTAGTCATCTTCTCTAGATCTCCCAAGCTCGAAGTAACAGTCACCAATGCGTCTATGACCCAATTCAATCTCAACAGCAACAACACTCTCTACTACAACCTCGCCGTCGATGTCGCCATCAGAAACCCTAGCCCAACCTTCAGGGTACACTACAATCGCATCGAAGTCGCGGTGAAATATAGGGAGCAGAAATTTGCTTTGGTGACTTTGAGTTTGCCACCCTTTGACCAAGGAACCAAGAACATAACCATTCTGCATCCAGTTCTTCAAGGGCAACCCTCTAGCCTGGAGACCGATGCAGGCATTTACAGTATTGACCTGCAGTTTTCTTTTCGGATAAGCCGCCGGGTTCTCAAAGGAATCGTTTACAAACCAGTAGGGATGTGTATGTTGAAGGTTCCGTCCAGTTCTAATGAAACAACTCCCGGTAGTAGGTTCAAGGCAACAGAATGTTTGGTGACGAGACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

185

Amino Acids

20.57

Weight (kDa)

9.78

Isoelectric Point (pI)

45.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 67 - 115 2.5e-06 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 414
AccII CGCG 1 cut(s) 257
AciI CCGC 3 cut(s) 54, 257, 430
AcsI RAATTY 1 cut(s) 278
AcuI CTGAAG 1 cut(s) 211
AfaI GTAC 1 cut(s) 234
AfiI CCNNNNNNNGG 1 cut(s) 519
AflIII ACRYGT 1 cut(s) 5
AgsI TTSAA 4 cut(s) 154, 356, 481, 526
AjnI CCWGG 1 cut(s) 372
AjuI GAANNNNNNNTTGG 2 cut(s) 269, 301
AluBI AGCT 2 cut(s) 30, 115
AluI AGCT 2 cut(s) 30, 115
Alw26I GTCTC 2 cut(s) 371, 544
ApoI RAATTY 1 cut(s) 278
ArsI GACNNNNNNTTYG 4 cut(s) 111, 143, 283, 315
AsuC2I CCSGG 2 cut(s) 434, 513
AsuHPI GGTGA 4 cut(s) 121, 271, 301, 556
BccI CCATC 2 cut(s) 65, 212
BceAI ACGGC 2 cut(s) 47, 176
BcgI CGANNNNNNTGC 2 cut(s) 235, 269
BciT130I CCWGG 1 cut(s) 374
BcnI CCSGG 2 cut(s) 434, 513
BcoDI GTCTC 2 cut(s) 371, 544
BfaI CTAG 3 cut(s) 102, 216, 369
BfmI CTRYAG 1 cut(s) 407
BfuAI ACCTGC 1 cut(s) 414
BglII AGATCT 1 cut(s) 104
BisI GCNGC 1 cut(s) 430
BlsI GCNGC 1 cut(s) 431
Bme1390I CCNGG 3 cut(s) 374, 434, 513
BmiI GGNNCC 2 cut(s) 322, 486
BmrFI CCNGG 3 cut(s) 374, 434, 513
BmsI GCATC 3 cut(s) 255, 352, 373
BpmI CTGGAG 1 cut(s) 395
BpuMI CCSGG 2 cut(s) 434, 513
BsaI GGTCTC 1 cut(s) 371
BsaJI CCNNGG 1 cut(s) 316
Bsc4I CCNNNNNNNGG 1 cut(s) 519
Bse1I ACTGG 3 cut(s) 347, 461, 492
BseBI CCWGG 1 cut(s) 374
BseDI CCNNGG 1 cut(s) 316
BseGI GGATG 2 cut(s) 343, 474
BseLI CCNNNNNNNGG 1 cut(s) 519
BseNI ACTGG 3 cut(s) 347, 461, 492
Bsh1236I CGCG 1 cut(s) 257
BsiSI CCGG 3 cut(s) 72, 433, 513
BslI CCNNNNNNNGG 1 cut(s) 519
BsmAI GTCTC 2 cut(s) 371, 544
BsmI GAATGC 1 cut(s) 83
Bso31I GGTCTC 1 cut(s) 371
Bsp143I GATC 1 cut(s) 104
BspACI CCGC 3 cut(s) 54, 257, 430
BspFNI CGCG 1 cut(s) 257
BspLI GGNNCC 2 cut(s) 322, 486
BspMAI CTGCAG 1 cut(s) 411
BspMI ACCTGC 1 cut(s) 414
BspTNI GGTCTC 1 cut(s) 371
BsrI ACTGG 3 cut(s) 347, 461, 492
BssECI CCNNGG 1 cut(s) 316
BssMI GATC 1 cut(s) 104
BssT1I CCWWGG 1 cut(s) 316
Bst2UI CCWGG 1 cut(s) 374
Bst4CI ACNGT 2 cut(s) 127, 398
BstC8I GCNNGC 1 cut(s) 388
BstDEI CTNAG 1 cut(s) 67
BstF5I GGATG 2 cut(s) 343, 474
BstFNI CGCG 1 cut(s) 257
BstKTI GATC 1 cut(s) 107
BstMAI GTCTC 2 cut(s) 371, 544
BstMBI GATC 1 cut(s) 104
BstMWI GCNNNNNNNGC 1 cut(s) 68
BstNI CCWGG 1 cut(s) 374
BstNSI RCATGY 1 cut(s) 9
BstSCI CCNGG 3 cut(s) 372, 432, 511
BstSFI CTRYAG 1 cut(s) 407
BstUI CGCG 1 cut(s) 257
BstX2I RGATCY 1 cut(s) 104
BstYI RGATCY 1 cut(s) 104
BtgZI GCGATG 1 cut(s) 43
BtsCI GGATG 2 cut(s) 343, 474
BveI ACCTGC 1 cut(s) 414
Cac8I GCNNGC 1 cut(s) 388
CseI GACGC 1 cut(s) 126
Csp6I GTAC 1 cut(s) 233
CviAII CATG 1 cut(s) 6
CviJI RGCY 6 cut(s) 30, 71, 115, 219, 372, 429
CviKI_1 RGCY 6 cut(s) 30, 71, 115, 219, 372, 429
CviQI GTAC 1 cut(s) 233
DdeI CTNAG 1 cut(s) 67
DpnI GATC 1 cut(s) 106
DpnII GATC 1 cut(s) 104
Eco130I CCWWGG 1 cut(s) 316
Eco31I GGTCTC 1 cut(s) 371
Eco57I CTGAAG 1 cut(s) 211
EcoRII CCWGG 1 cut(s) 372
EcoT14I CCWWGG 1 cut(s) 316
ErhI CCWWGG 1 cut(s) 316
FaeI CATG 1 cut(s) 9
FaiI YATR 7 cut(s) 7, 47, 80, 143, 267, 332, 476
FatI CATG 1 cut(s) 5
Fnu4HI GCNGC 1 cut(s) 430
FokI GGATG 2 cut(s) 330, 481
Fsp4HI GCNGC 1 cut(s) 430
FspBI CTAG 3 cut(s) 102, 216, 369
GluI GCNGC 1 cut(s) 430
GsuI CTGGAG 1 cut(s) 395
HapII CCGG 3 cut(s) 72, 433, 513
HgaI GACGC 1 cut(s) 126
Hin1II CATG 1 cut(s) 9
HinfI GANTC 2 cut(s) 75, 447
HpaII CCGG 3 cut(s) 72, 433, 513
HphI GGTGA 4 cut(s) 121, 271, 301, 556
Hpy166II GTNNAC 2 cut(s) 235, 454
Hpy188I TCNGA 2 cut(s) 209, 423
Hpy188III TCNNGA 1 cut(s) 102
Hpy8I GTNNAC 2 cut(s) 235, 454
Hpy99I CGWCG 1 cut(s) 197
HpyAV CCTTC 2 cut(s) 235, 475
HpyCH4III ACNGT 2 cut(s) 127, 398
HpyCH4V TGCA 3 cut(s) 343, 386, 409
HpyF10VI GCNNNNNNNGC 1 cut(s) 68
HpyF3I CTNAG 1 cut(s) 67
Hsp92II CATG 1 cut(s) 9
Kzo9I GATC 1 cut(s) 104
LmnI GCTCC 1 cut(s) 271
LweI GCATC 3 cut(s) 255, 352, 373
MaeI CTAG 3 cut(s) 102, 216, 369
MaeIII GTNAC 5 cut(s) 8, 121, 127, 289, 544
MalI GATC 1 cut(s) 106
MboI GATC 1 cut(s) 104
MboII GAAGA 2 cut(s) 88, 344
MflI RGATCY 1 cut(s) 104
MluCI AATT 2 cut(s) 149, 278
MlyI GAGTC 1 cut(s) 84
MnlI CCTC 2 cut(s) 197, 376
MspI CCGG 3 cut(s) 72, 433, 513
MspR9I CCNGG 3 cut(s) 374, 434, 513
Mva1269I GAATGC 1 cut(s) 83
MvaI CCWGG 1 cut(s) 374
MvnI CGCG 1 cut(s) 257
MwoI GCNNNNNNNGC 1 cut(s) 68
NciI CCSGG 2 cut(s) 434, 513
NdeII GATC 1 cut(s) 104
NlaIII CATG 1 cut(s) 9
NlaIV GGNNCC 2 cut(s) 322, 486
NmuCI GTSAC 3 cut(s) 127, 289, 544
NspI RCATGY 1 cut(s) 9
PciI ACATGT 1 cut(s) 5
PctI GAATGC 1 cut(s) 83
PfeI GAWTC 1 cut(s) 447
PkrI GCNGC 1 cut(s) 431
PleI GAGTC 1 cut(s) 83
PpsI GAGTC 1 cut(s) 83
PscI ACATGT 1 cut(s) 5
Psp6I CCWGG 1 cut(s) 372
PspGI CCWGG 1 cut(s) 372
PspN4I GGNNCC 2 cut(s) 322, 486
PstI CTGCAG 1 cut(s) 411
PsuI RGATCY 1 cut(s) 104
RsaI GTAC 1 cut(s) 234
RsaNI GTAC 1 cut(s) 233
SatI GCNGC 1 cut(s) 430
Sau3AI GATC 1 cut(s) 104
SchI GAGTC 1 cut(s) 84
ScrFI CCNGG 3 cut(s) 374, 434, 513
SetI ASST 7 cut(s) 32, 117, 189, 227, 408, 486, 524
SfaNI GCATC 3 cut(s) 255, 352, 373
SfcI CTRYAG 1 cut(s) 407
Sse9I AATT 2 cut(s) 149, 278
SsiI CCGC 3 cut(s) 54, 257, 430
SspMI CTAG 3 cut(s) 102, 216, 369
StyD4I CCNGG 3 cut(s) 372, 432, 511
StyI CCWWGG 1 cut(s) 316
TaaI ACNGT 2 cut(s) 127, 398
TaqI TCGA 3 cut(s) 117, 195, 249
TaqII GACCGA 1 cut(s) 395
TasI AATT 2 cut(s) 149, 278
TauI GCSGC 1 cut(s) 432
TfiI GAWTC 1 cut(s) 447
TseFI GTSAC 3 cut(s) 127, 289, 544
Tsp45I GTSAC 3 cut(s) 127, 289, 544
TspDTI ATGAA 1 cut(s) 516
TspGWI ACGGA 2 cut(s) 27, 477
XapI RAATTY 1 cut(s) 278
XbaI TCTAGA 1 cut(s) 101
XceI RCATGY 1 cut(s) 9
XspI CTAG 3 cut(s) 102, 216, 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.