RLG00000024028

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0001039
Physical Location & Seq
Reverse (-)
30963900 .. 30965047
1148 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024028

Sequence Viewer

Length: 621 bp
ATGGCTGTCCCTTGCATGCTTTGGCTTGAGTTTACATGCATTAATATTCCTTTGGCCATGACTTGCTCCATGACATGTAACGGAAAGAAACTAACAAGCTGCACTATTCTCTCTATATTTACCGCCATCGCCGTCTTAGCCGGAGTAATAGCATTCGTAATCATCTTCTCTAGATCTCCCAAGCTCGAAGTAACAGTCACCAATGCGTCTATGACCCAATTCAATCTCAACAGCAGCAACAACACTCTCTACTACAACCTCGCCCTCGATGTCGCCATCAGAAACCCTAGCCCAACCTTCAGGGTACATTACAATCGCATCGAAGTCGCGGCGAAATATAGGGAGCAGAAATTTGCTTTGGTGACTTTGAGTTCGCCACCCTTTGACCAAGGAACCAAGAACATAACCATTCTGCATCCAGTTCTTCAAGGGCAACCCTCTAGCCTGGAGACCGATGCAGGCATTTACAGTATTGACCTGCAGTTTTCTTTTCGGATAAGCCGCCGGGTTCTCAAAGGAATCGTTTACAAACCAGTAGGGATGTGTATGTTGAAGGTTCCGTCGAGTTCTAATGAAACAACTCCCGATCGTAAGGAACGAATGAATCTTAGGAAGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

207

Amino Acids

22.97

Weight (kDa)

9.53

Isoelectric Point (pI)

51.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 91 - 139 5.3e-06 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 486
AccII CGCG 1 cut(s) 329
AciI CCGC 3 cut(s) 123, 329, 502
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 1 cut(s) 350
AcuI CTGAAG 1 cut(s) 283
AfaI GTAC 1 cut(s) 306
AflIII ACRYGT 1 cut(s) 74
AgsI TTSAA 3 cut(s) 223, 428, 553
AjnI CCWGG 1 cut(s) 444
AjuI GAANNNNNNNTTGG 2 cut(s) 341, 373
AluBI AGCT 2 cut(s) 99, 184
AluI AGCT 2 cut(s) 99, 184
Alw26I GTCTC 1 cut(s) 443
AoxI GGCC 1 cut(s) 54
ApeKI GCWGC 2 cut(s) 99, 234
ApoI RAATTY 1 cut(s) 350
ArsI GACNNNNNNTTYG 4 cut(s) 180, 212, 355, 387
AseI ATTAAT 1 cut(s) 42
AsuC2I CCSGG 1 cut(s) 506
AsuHPI GGTGA 2 cut(s) 190, 373
BalI TGGCCA 1 cut(s) 56
BbvI GCAGC 2 cut(s) 86, 246
BccI CCATC 2 cut(s) 134, 284
BceAI ACGGC 1 cut(s) 116
BcgI CGANNNNNNTGC 2 cut(s) 307, 341
BciT130I CCWGG 1 cut(s) 446
BcnI CCSGG 1 cut(s) 506
BcoDI GTCTC 1 cut(s) 443
BfaI CTAG 3 cut(s) 171, 288, 441
BfmI CTRYAG 1 cut(s) 479
BfuAI ACCTGC 1 cut(s) 486
BglII AGATCT 1 cut(s) 173
BisI GCNGC 4 cut(s) 100, 235, 330, 502
BlsI GCNGC 4 cut(s) 101, 236, 331, 503
Bme1390I CCNGG 2 cut(s) 446, 506
BmiI GGNNCC 2 cut(s) 394, 558
BmrFI CCNGG 2 cut(s) 446, 506
BmsI GCATC 3 cut(s) 327, 424, 445
BpmI CTGGAG 1 cut(s) 467
BpuEI CTTGAG 1 cut(s) 47
BpuMI CCSGG 1 cut(s) 506
BsaI GGTCTC 1 cut(s) 443
BsaJI CCNNGG 1 cut(s) 388
Bse1I ACTGG 2 cut(s) 419, 533
BseBI CCWGG 1 cut(s) 446
BseDI CCNNGG 1 cut(s) 388
BseGI GGATG 2 cut(s) 415, 546
BseNI ACTGG 2 cut(s) 419, 533
BseXI GCAGC 2 cut(s) 86, 246
BsgI GTGCAG 1 cut(s) 85
Bsh1236I CGCG 1 cut(s) 329
Bsh1285I CGRYCG 1 cut(s) 589
BshFI GGCC 1 cut(s) 56
BsiEI CGRYCG 1 cut(s) 589
BsiSI CCGG 2 cut(s) 141, 505
BsmAI GTCTC 1 cut(s) 443
BsmI GAATGC 1 cut(s) 152
BsnI GGCC 1 cut(s) 56
Bso31I GGTCTC 1 cut(s) 443
Bsp143I GATC 2 cut(s) 173, 586
BspACI CCGC 3 cut(s) 123, 329, 502
BspANI GGCC 1 cut(s) 56
BspFNI CGCG 1 cut(s) 329
BspLI GGNNCC 2 cut(s) 394, 558
BspMAI CTGCAG 1 cut(s) 483
BspMI ACCTGC 1 cut(s) 486
BspTNI GGTCTC 1 cut(s) 443
BsrI ACTGG 2 cut(s) 419, 533
BssECI CCNNGG 1 cut(s) 388
BssMI GATC 2 cut(s) 173, 586
BssT1I CCWWGG 1 cut(s) 388
Bst2UI CCWGG 1 cut(s) 446
Bst4CI ACNGT 2 cut(s) 196, 470
Bst6I CTCTTC 1 cut(s) 608
BstC8I GCNNGC 2 cut(s) 17, 460
BstDEI CTNAG 2 cut(s) 136, 608
BstF5I GGATG 2 cut(s) 415, 546
BstFNI CGCG 1 cut(s) 329
BstKTI GATC 2 cut(s) 176, 589
BstMAI GTCTC 1 cut(s) 443
BstMBI GATC 2 cut(s) 173, 586
BstMCI CGRYCG 1 cut(s) 589
BstMWI GCNNNNNNNGC 1 cut(s) 137
BstNI CCWGG 1 cut(s) 446
BstNSI RCATGY 3 cut(s) 19, 39, 78
BstSCI CCNGG 2 cut(s) 444, 504
BstSFI CTRYAG 1 cut(s) 479
BstUI CGCG 1 cut(s) 329
BstV1I GCAGC 2 cut(s) 86, 246
BstX2I RGATCY 1 cut(s) 173
BstYI RGATCY 1 cut(s) 173
BsuRI GGCC 1 cut(s) 56
BtgZI GCGATG 1 cut(s) 112
BtsCI GGATG 2 cut(s) 415, 546
BveI ACCTGC 1 cut(s) 486
Cac8I GCNNGC 2 cut(s) 17, 460
CseI GACGC 1 cut(s) 195
Csp6I GTAC 1 cut(s) 305
CviAII CATG 5 cut(s) 16, 36, 58, 70, 75
CviJI RGCY 9 cut(s) 5, 25, 56, 99, 140, 184, 291, 444, 501
CviKI_1 RGCY 9 cut(s) 5, 25, 56, 99, 140, 184, 291, 444, 501
CviQI GTAC 1 cut(s) 305
DdeI CTNAG 2 cut(s) 136, 608
DpnI GATC 2 cut(s) 175, 588
DpnII GATC 2 cut(s) 173, 586
EaeI YGGCCR 1 cut(s) 54
Eam1104I CTCTTC 1 cut(s) 608
EarI CTCTTC 1 cut(s) 608
Eco130I CCWWGG 1 cut(s) 388
Eco31I GGTCTC 1 cut(s) 443
Eco57I CTGAAG 1 cut(s) 283
EcoRII CCWGG 1 cut(s) 444
EcoT14I CCWWGG 1 cut(s) 388
EcoT22I ATGCAT 1 cut(s) 41
ErhI CCWWGG 1 cut(s) 388
FaeI CATG 5 cut(s) 19, 39, 61, 73, 78
FatI CATG 5 cut(s) 15, 35, 57, 69, 74
Fnu4HI GCNGC 4 cut(s) 100, 235, 330, 502
FokI GGATG 2 cut(s) 402, 553
Fsp4HI GCNGC 4 cut(s) 100, 235, 330, 502
FspBI CTAG 3 cut(s) 171, 288, 441
GluI GCNGC 4 cut(s) 100, 235, 330, 502
GsuI CTGGAG 1 cut(s) 467
HaeIII GGCC 1 cut(s) 56
HapII CCGG 2 cut(s) 141, 505
HgaI GACGC 1 cut(s) 195
Hin1II CATG 5 cut(s) 19, 39, 61, 73, 78
HinfI GANTC 2 cut(s) 519, 604
HpaII CCGG 2 cut(s) 141, 505
HphI GGTGA 2 cut(s) 190, 373
Hpy166II GTNNAC 2 cut(s) 33, 526
Hpy188I TCNGA 2 cut(s) 281, 495
Hpy188III TCNNGA 2 cut(s) 171, 584
Hpy8I GTNNAC 2 cut(s) 33, 526
Hpy99I CGWCG 1 cut(s) 565
HpyAV CCTTC 2 cut(s) 307, 547
HpyCH4III ACNGT 2 cut(s) 196, 470
HpyCH4V TGCA 6 cut(s) 15, 39, 102, 415, 458, 481
HpyF10VI GCNNNNNNNGC 1 cut(s) 137
HpyF3I CTNAG 2 cut(s) 136, 608
Hsp92II CATG 5 cut(s) 19, 39, 61, 73, 78
Kzo9I GATC 2 cut(s) 173, 586
LmnI GCTCC 2 cut(s) 71, 343
LpnPI CCDG 9 cut(s) 154, 286, 431, 432, 444, 458, 491, 518, 546
Lsp1109I GCAGC 2 cut(s) 86, 246
LweI GCATC 3 cut(s) 327, 424, 445
MaeI CTAG 3 cut(s) 171, 288, 441
MaeIII GTNAC 4 cut(s) 77, 190, 196, 361
MalI GATC 2 cut(s) 175, 588
MboI GATC 2 cut(s) 173, 586
MboII GAAGA 2 cut(s) 157, 416
MflI RGATCY 1 cut(s) 173
MlsI TGGCCA 1 cut(s) 56
MluCI AATT 2 cut(s) 218, 350
MluNI TGGCCA 1 cut(s) 56
MnlI CCTC 3 cut(s) 269, 275, 448
Mox20I TGGCCA 1 cut(s) 56
Mph1103I ATGCAT 1 cut(s) 41
MscI TGGCCA 1 cut(s) 56
MseI TTAA 1 cut(s) 42
Msp20I TGGCCA 1 cut(s) 56
MspI CCGG 2 cut(s) 141, 505
MspR9I CCNGG 2 cut(s) 446, 506
Mva1269I GAATGC 1 cut(s) 152
MvaI CCWGG 1 cut(s) 446
MvnI CGCG 1 cut(s) 329
MwoI GCNNNNNNNGC 1 cut(s) 137
NciI CCSGG 1 cut(s) 506
NdeII GATC 2 cut(s) 173, 586
NlaIII CATG 5 cut(s) 19, 39, 61, 73, 78
NlaIV GGNNCC 2 cut(s) 394, 558
NmuCI GTSAC 2 cut(s) 196, 361
NsiI ATGCAT 1 cut(s) 41
NspI RCATGY 3 cut(s) 19, 39, 78
PaeI GCATGC 1 cut(s) 19
PciI ACATGT 1 cut(s) 74
PcsI WCGNNNNNNNCGW 1 cut(s) 595
PctI GAATGC 1 cut(s) 152
PfeI GAWTC 2 cut(s) 519, 604
PkrI GCNGC 4 cut(s) 101, 236, 331, 503
Ple19I CGATCG 1 cut(s) 589
PscI ACATGT 1 cut(s) 74
PshBI ATTAAT 1 cut(s) 42
Psp6I CCWGG 1 cut(s) 444
PspGI CCWGG 1 cut(s) 444
PspN4I GGNNCC 2 cut(s) 394, 558
PstI CTGCAG 1 cut(s) 483
PsuI RGATCY 1 cut(s) 173
PvuI CGATCG 1 cut(s) 589
RsaI GTAC 1 cut(s) 306
RsaNI GTAC 1 cut(s) 305
SaqAI TTAA 1 cut(s) 42
SatI GCNGC 4 cut(s) 100, 235, 330, 502
Sau3AI GATC 2 cut(s) 173, 586
ScrFI CCNGG 2 cut(s) 446, 506
SetI ASST 6 cut(s) 101, 186, 261, 299, 480, 558
SfaNI GCATC 3 cut(s) 327, 424, 445
SfcI CTRYAG 1 cut(s) 479
SmlI CTYRAG 1 cut(s) 26
SmoI CTYRAG 1 cut(s) 26
SphI GCATGC 1 cut(s) 19
Sse9I AATT 2 cut(s) 218, 350
SsiI CCGC 3 cut(s) 123, 329, 502
SspI AATATT 1 cut(s) 46
SspMI CTAG 3 cut(s) 171, 288, 441
StyD4I CCNGG 2 cut(s) 444, 504
StyI CCWWGG 1 cut(s) 388
TaaI ACNGT 2 cut(s) 196, 470
TaqI TCGA 4 cut(s) 186, 267, 321, 563
TaqII GACCGA 1 cut(s) 467
TasI AATT 2 cut(s) 218, 350
TauI GCSGC 2 cut(s) 332, 504
TfiI GAWTC 2 cut(s) 519, 604
Tru1I TTAA 1 cut(s) 42
Tru9I TTAA 1 cut(s) 42
TseFI GTSAC 2 cut(s) 196, 361
TseI GCWGC 2 cut(s) 99, 234
Tsp45I GTSAC 2 cut(s) 196, 361
TspDTI ATGAA 2 cut(s) 588, 617
TspGWI ACGGA 2 cut(s) 96, 549
VspI ATTAAT 1 cut(s) 42
XapI RAATTY 1 cut(s) 350
XbaI TCTAGA 1 cut(s) 170
XceI RCATGY 3 cut(s) 19, 39, 78
XspI CTAG 3 cut(s) 171, 288, 441
Zsp2I ATGCAT 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.