Rh3AG183200

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0001039
Physical Location & Seq
Forward (+)
17325391 .. 17326395
1005 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG183200.1

Sequence Viewer

Length: 609 bp
ATGACTGGAAGGCGTGCCTGCATCGACTGCAGCTTCTGCCTATTCTGCAACACCATCCTCTGCTTCACCCTAGTCTTCTCCATAGTGTGGTTCATCTTCCTTCCCCAAGAGCCCAAATTCAGCATCACTAATGCCTCTCTAACCAATTTCGACTTCATCGGGACCACCAGAACTCTCCACTACAACCTTGAGCTCAACATTACCATCAGAAATCCCAAGAAGGTTGACATATACTACAATAGCATCCAAGTCATCGCTAAATATAAAAAAAAGGAGTTTGCCATGCTGACTGTAGATTCCACACCATTTTACCAAGGCCACAAGAACACAACCATTTTGGATCATGTGGTACTTGAAGGGCAGCAATCAGTGGAGTTTGGGGAGCCAGAGGTTTCCAAATTTAAGGCAGAGACTGCTGCTGGGTTTTACAGTATCGATGTGCAACTTGTTCTTCAAGTAAAGCTCGGGTATGACATGTTCAAGACAAGGTATTACCAGCAGTCAGGCGGCAAGATCGATTGCAAGCTGAAGGTTCCTTTGAGACATGCTAGACGTTTCAAAACTACAAAGTGTGGAAATGTATACATTATTTCAGATCCTGTTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

202

Amino Acids

23.28

Weight (kDa)

9.11

Isoelectric Point (pI)

38.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 67 - 154 2.5e-06 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 87
AccI GTMKAC 1 cut(s) 582
AciI CCGC 1 cut(s) 507
AclWI GGATC 2 cut(s) 348, 590
AcsI RAATTY 2 cut(s) 116, 398
AcuI CTGAAG 1 cut(s) 548
AfaI GTAC 1 cut(s) 351
AfiI CCNNNNNNNGG 1 cut(s) 87
AflIII ACRYGT 1 cut(s) 474
AgsI TTSAA 4 cut(s) 356, 455, 481, 559
AjuI GAANNNNNNNTTGG 2 cut(s) 137, 169
AluBI AGCT 4 cut(s) 33, 193, 463, 526
AluI AGCT 4 cut(s) 33, 193, 463, 526
Alw21I GWGCWC 1 cut(s) 195
Alw26I GTCTC 2 cut(s) 404, 535
AlwI GGATC 2 cut(s) 348, 590
AlwNI CAGNNNCTG 3 cut(s) 36, 413, 599
Ama87I CYCGRG 1 cut(s) 464
AoxI GGCC 1 cut(s) 316
ApeKI GCWGC 3 cut(s) 30, 361, 416
ApoI RAATTY 2 cut(s) 116, 398
AspS9I GGNCC 1 cut(s) 162
AsuHPI GGTGA 1 cut(s) 58
AvaI CYCGRG 1 cut(s) 464
AvaII GGWCC 1 cut(s) 162
BanII GRGCYC 2 cut(s) 114, 195
BbsI GAAGAC 1 cut(s) 67
Bbv12I GWGCWC 1 cut(s) 195
BbvI GCAGC 3 cut(s) 42, 373, 403
BccI CCATC 2 cut(s) 62, 212
BcoDI GTCTC 2 cut(s) 404, 535
BfaI CTAG 2 cut(s) 71, 549
BfmI CTRYAG 2 cut(s) 28, 291
BisI GCNGC 4 cut(s) 31, 362, 417, 508
BlsI GCNGC 4 cut(s) 32, 363, 418, 509
Bme18I GGWCC 1 cut(s) 162
BmeT110I CYCGRG 1 cut(s) 464
BmgT120I GGNCC 1 cut(s) 162
BmiI GGNNCC 3 cut(s) 163, 384, 534
BmsI GCATC 3 cut(s) 30, 132, 252
BpiI GAAGAC 1 cut(s) 67
BpuEI CTTGAG 1 cut(s) 209
Bsa29I ATCGAT 2 cut(s) 435, 516
BsaJI CCNNGG 1 cut(s) 313
Bsc4I CCNNNNNNNGG 1 cut(s) 87
Bse1I ACTGG 1 cut(s) 10
BseCI ATCGAT 2 cut(s) 435, 516
BseDI CCNNGG 1 cut(s) 313
BseGI GGATG 2 cut(s) 54, 243
BseLI CCNNNNNNNGG 1 cut(s) 87
BseNI ACTGG 1 cut(s) 10
BseXI GCAGC 3 cut(s) 42, 373, 403
BseYI CCCAGC 1 cut(s) 419
BshFI GGCC 1 cut(s) 318
BshVI ATCGAT 2 cut(s) 435, 516
BsiHKAI GWGCWC 1 cut(s) 195
BsiHKCI CYCGRG 1 cut(s) 464
BslFI GGGAC 1 cut(s) 175
BslI CCNNNNNNNGG 1 cut(s) 87
BsmAI GTCTC 2 cut(s) 404, 535
BsmFI GGGAC 1 cut(s) 175
BsnI GGCC 1 cut(s) 318
BsoBI CYCGRG 1 cut(s) 464
Bsp1286I GDGCHC 2 cut(s) 114, 195
Bsp143I GATC 3 cut(s) 340, 513, 595
BspACI CCGC 1 cut(s) 507
BspANI GGCC 1 cut(s) 318
BspDI ATCGAT 2 cut(s) 435, 516
BspLI GGNNCC 3 cut(s) 163, 384, 534
BspMAI CTGCAG 1 cut(s) 32
BspPI GGATC 2 cut(s) 348, 590
BsrI ACTGG 1 cut(s) 10
BssECI CCNNGG 1 cut(s) 313
BssMI GATC 3 cut(s) 340, 513, 595
BssNAI GTATAC 1 cut(s) 583
BssT1I CCWWGG 1 cut(s) 313
Bst1107I GTATAC 1 cut(s) 583
Bst4CI ACNGT 2 cut(s) 292, 431
BstAPI GCANNNNNTGC 3 cut(s) 27, 36, 413
BstC8I GCNNGC 3 cut(s) 15, 19, 524
BstF5I GGATG 2 cut(s) 54, 243
BstKTI GATC 3 cut(s) 343, 516, 598
BstMAI GTCTC 2 cut(s) 404, 535
BstMBI GATC 3 cut(s) 340, 513, 595
BstMWI GCNNNNNNNGC 4 cut(s) 27, 36, 45, 413
BstNSI RCATGY 2 cut(s) 478, 548
BstSFI CTRYAG 2 cut(s) 28, 291
BstV1I GCAGC 3 cut(s) 42, 373, 403
BstV2I GAAGAC 1 cut(s) 67
BstX2I RGATCY 1 cut(s) 595
BstYI RGATCY 1 cut(s) 595
BstZ17I GTATAC 1 cut(s) 583
Bsu15I ATCGAT 2 cut(s) 435, 516
BsuRI GGCC 1 cut(s) 318
BsuTUI ATCGAT 2 cut(s) 435, 516
BtgZI GCGATG 1 cut(s) 238
BtsCI GGATG 2 cut(s) 54, 243
BtsIMutI CAGTG 1 cut(s) 375
Cac8I GCNNGC 3 cut(s) 15, 19, 524
CaiI CAGNNNCTG 3 cut(s) 36, 413, 599
Cfr13I GGNCC 1 cut(s) 162
ClaI ATCGAT 2 cut(s) 435, 516
Csp6I GTAC 1 cut(s) 350
CviAII CATG 4 cut(s) 283, 344, 475, 545
CviJI RGCY 7 cut(s) 33, 112, 193, 318, 385, 463, 526
CviKI_1 RGCY 7 cut(s) 33, 112, 193, 318, 385, 463, 526
CviQI GTAC 1 cut(s) 350
DpnI GATC 3 cut(s) 342, 515, 597
DpnII GATC 3 cut(s) 340, 513, 595
Ecl136II GAGCTC 1 cut(s) 193
Eco130I CCWWGG 1 cut(s) 313
Eco24I GRGCYC 2 cut(s) 114, 195
Eco47I GGWCC 1 cut(s) 162
Eco53kI GAGCTC 1 cut(s) 193
Eco57I CTGAAG 1 cut(s) 548
Eco88I CYCGRG 1 cut(s) 464
EcoICRI GAGCTC 1 cut(s) 193
EcoT14I CCWWGG 1 cut(s) 313
EcoT38I GRGCYC 2 cut(s) 114, 195
ErhI CCWWGG 1 cut(s) 313
FaeI CATG 4 cut(s) 286, 347, 478, 548
FaqI GGGAC 1 cut(s) 175
FatI CATG 4 cut(s) 282, 343, 474, 544
FblI GTMKAC 1 cut(s) 582
Fnu4HI GCNGC 4 cut(s) 31, 362, 417, 508
FokI GGATG 2 cut(s) 41, 230
FriOI GRGCYC 2 cut(s) 114, 195
Fsp4HI GCNGC 4 cut(s) 31, 362, 417, 508
FspBI CTAG 2 cut(s) 71, 549
GluI GCNGC 4 cut(s) 31, 362, 417, 508
GsaI CCCAGC 1 cut(s) 423
HaeIII GGCC 1 cut(s) 318
Hin1II CATG 4 cut(s) 286, 347, 478, 548
HincII GTYRAC 1 cut(s) 226
HindII GTYRAC 1 cut(s) 226
HinfI GANTC 1 cut(s) 296
HphI GGTGA 1 cut(s) 58
Hpy166II GTNNAC 2 cut(s) 226, 583
Hpy188I TCNGA 3 cut(s) 209, 595, 608
Hpy188III TCNNGA 2 cut(s) 160, 481
Hpy8I GTNNAC 2 cut(s) 226, 583
HpyAV CCTTC 5 cut(s) 3, 110, 214, 350, 523
HpyCH4III ACNGT 2 cut(s) 292, 431
HpyCH4IV ACGT 1 cut(s) 553
HpyCH4V TGCA 5 cut(s) 21, 30, 48, 442, 522
HpyF10VI GCNNNNNNNGC 4 cut(s) 27, 36, 45, 413
HpySE526I ACGT 1 cut(s) 553
Hsp92II CATG 4 cut(s) 286, 347, 478, 548
Kzo9I GATC 3 cut(s) 340, 513, 595
LmnI GCTCC 1 cut(s) 382
LpnPI CCDG 6 cut(s) 31, 181, 399, 405, 489, 509
Lsp1109I GCAGC 3 cut(s) 42, 373, 403
LweI GCATC 3 cut(s) 30, 132, 252
MaeI CTAG 2 cut(s) 71, 549
MaeII ACGT 1 cut(s) 553
MalI GATC 3 cut(s) 342, 515, 597
MboI GATC 3 cut(s) 340, 513, 595
MboII GAAGA 3 cut(s) 67, 88, 443
MflI RGATCY 1 cut(s) 595
MhlI GDGCHC 2 cut(s) 114, 195
MluCI AATT 3 cut(s) 116, 145, 398
MnlI CCTC 3 cut(s) 68, 145, 382
MseI TTAA 1 cut(s) 402
MwoI GCNNNNNNNGC 4 cut(s) 27, 36, 45, 413
NdeII GATC 3 cut(s) 340, 513, 595
NlaIII CATG 4 cut(s) 286, 347, 478, 548
NlaIV GGNNCC 3 cut(s) 163, 384, 534
NspI RCATGY 2 cut(s) 478, 548
PciI ACATGT 1 cut(s) 474
PfeI GAWTC 1 cut(s) 296
PflMI CCANNNNNTGG 1 cut(s) 87
PkrI GCNGC 4 cut(s) 32, 363, 418, 509
PscI ACATGT 1 cut(s) 474
Psp124BI GAGCTC 1 cut(s) 195
PspFI CCCAGC 1 cut(s) 419
PspN4I GGNNCC 3 cut(s) 163, 384, 534
PspPI GGNCC 1 cut(s) 162
PsrI GAACNNNNNNTAC 2 cut(s) 461, 493
PstI CTGCAG 1 cut(s) 32
PstNI CAGNNNCTG 3 cut(s) 36, 413, 599
PsuI RGATCY 1 cut(s) 595
RsaI GTAC 1 cut(s) 351
RsaNI GTAC 1 cut(s) 350
SacI GAGCTC 1 cut(s) 195
SaqAI TTAA 1 cut(s) 402
SatI GCNGC 4 cut(s) 31, 362, 417, 508
Sau3AI GATC 3 cut(s) 340, 513, 595
Sau96I GGNCC 1 cut(s) 162
SduI GDGCHC 2 cut(s) 114, 195
SfaNI GCATC 3 cut(s) 30, 132, 252
SfcI CTRYAG 2 cut(s) 28, 291
SinI GGWCC 1 cut(s) 162
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
Sse9I AATT 3 cut(s) 116, 145, 398
SsiI CCGC 1 cut(s) 507
SspMI CTAG 2 cut(s) 71, 549
SstI GAGCTC 1 cut(s) 195
StyI CCWWGG 1 cut(s) 313
TaaI ACNGT 2 cut(s) 292, 431
TaiI ACGT 1 cut(s) 556
TaqI TCGA 4 cut(s) 24, 150, 435, 516
TasI AATT 3 cut(s) 116, 145, 398
TauI GCSGC 1 cut(s) 510
TfiI GAWTC 1 cut(s) 296
Tru1I TTAA 1 cut(s) 402
Tru9I TTAA 1 cut(s) 402
TscAI CASTG 1 cut(s) 375
TseI GCWGC 3 cut(s) 30, 361, 416
TspDTI ATGAA 2 cut(s) 82, 145
TspRI CASTG 1 cut(s) 375
Van91I CCANNNNNTGG 1 cut(s) 87
VpaK11BI GGWCC 1 cut(s) 162
XapI RAATTY 2 cut(s) 116, 398
XceI RCATGY 2 cut(s) 478, 548
XmiI GTMKAC 1 cut(s) 582
XspI CTAG 2 cut(s) 71, 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.