FvH4_6g50410

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000534
Physical Location & Seq
Reverse (-)
37518506 .. 37520974
2469 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g50410.t4

Sequence Viewer

Length: 1059 bp
ATGGCTCCTTGTACATGGGGTTCTGTACTGCCTTTTGTAGCCATGGTAATGGTGCAGTTCATAGATATAGGTGCAGCAACTATAAACAAAGCAGCCATGTCGAAAGGAATGAGCAGTTATGTCCTAGTTGTTTACTCTACTGCGCTTGCTACCGTCTTTCTCCTTCCTTGTTTCATCTTTCAAAAGAAGATTTCACTTGCTCTTTCACTGCTTGGTGGACAATTCCTTCTTGGCCTAATTGGGAGTTCAAACATGTTATTGGCATATAATGGCATCAACTTCAGCTCACCAACAGTTGCTTCAGCTATGGGAAACTGTATTCCAATTTTTACATTCATACTGGCAATCGTTTTCAGGATGGAAAAGCTGGATTTGAGGAGGTCAAGCTGTCAAGCCAAAGTGTTGGGGACTATTGTTTCAGTGTCTGGAGCATTTGTAGTAATACTATACAAGGGATCAGTAATCTTTTCACCATCCAACTCTCCTCACCAGAACTTGATGATGATCTTGCAGCAGTCAAATAAGTGGGTCATTGGAGGTCTTATGCTTGCAATGGCAAGCATTTTGAAGTCATCATGGAATATTCTTCAGACATCAATTGTTAAGAACTGTCCATCAATGATTACAGTACTCTTCTTCTACACCTTCTTTGTGACACTTCAATCTACAGTGATCTCTCTATTTTTGGAAAGCAATCCAAATGCGTGGGTACTAAGGCCTGACATTGAGATGATTTCCATTGTCTTCTCAGCTATATTTGGGAGTCTGTTCCATATTGGTATTGAAACTTGGTGCTTGCACCAAAAGGGCCCTGTCTTTGTAGCCATGTTCAGGCCCTTGGCAGTTCCCATTTCGGCAGTCATGGTAGTCATCTTCCTCGGCGAAGCACTTCATCTTGGCAGTGTGATTGGATCTGTCATGATTGCAATTGGGTTTTATGCTATGATGTGGGCACAAGTGAAAGAAAGTAGCTCCAGCGCTATAGAGAATGAAGTTCCAAACTTGGCAGCAACTCAAAAGACCCCTCTTTTGCAATGCAGCACCTCAGCAGATGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

353

Amino Acids

38.06

Weight (kDa)

8.97

Isoelectric Point (pI)

45.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 27 - 148 7.8e-10 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000534)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g50400 FvH4_6g50410 FvH4_6g50410 FvH4_6g50410 FvH4_6g50410 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420
malus_domestica MD09G1036400.v1.1 MD09G1036600.v1.1 MD17G1038100.v1.1 MD17G1038200.v1.1
prunus_persica Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G281000_v2.0.a1 Prupe.3G281000_v2.0.a1 Prupe.3G281000_v2.0.a1 Prupe.3G281000_v2.0.a1
pyrus_communis pycom111g02920 pycom111g02930 pycom111g02940 pycom111g02950 pycom16g07600 pycom17g03430 pycom17g03440
rosa_chinensis RchiOBHm_Chr2g0170801 RchiOBHm_Chr2g0170811 RchiOBHm_Chr2g0170821 RchiOBHm_Chr2g0170831 RchiOBHm_Chr2g0170841
rosa_laevigata RLG00000021981 RLG00000021983 RLG00000021984 RLG00000021985
rosa_multiflora Rmu_co8418849.1_g000001 Rmu_co8437261.1_g000001 Rmu_sc0008393.1_g000003
rosa_roxburghii Rroxscaffold_1G00030380 Rroxscaffold_2G00081040 Rroxscaffold_2G00081050 Rroxscaffold_2G00081060
rosa_rugosa Rorug02G0551300 Rorug02G0551400 Rorug02G0551700 Rorug02G0551800
rosa_samantha Rh2AG624300 Rh2BG634700 Rh2BG634800 Rh2BG634900 Rh2BG635000 Rh2CG603500 Rh2CG603600 Rh2CG603700 Rh2CG603800
rosa_wichuraiana Rw0G017070 Rw2G051700 Rw2G051710 Rw2G051720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 463, 919
AcuI CTGAAG 3 cut(s) 265, 285, 572
AfaI GTAC 4 cut(s) 13, 27, 630, 711
AfeI AGCGCT 1 cut(s) 979
AfiI CCNNNNNNNGG 1 cut(s) 831
AflIII ACRYGT 1 cut(s) 252
AgsI TTSAA 5 cut(s) 182, 249, 568, 662, 785
AjuI GAANNNNNNNTTGG 2 cut(s) 283, 315
AluBI AGCT 6 cut(s) 285, 305, 367, 387, 752, 972
AluI AGCT 6 cut(s) 285, 305, 367, 387, 752, 972
AlwI GGATC 2 cut(s) 463, 919
AlwNI CAGNNNCTG 1 cut(s) 425
Aor51HI AGCGCT 1 cut(s) 979
AoxI GGCC 4 cut(s) 232, 716, 808, 833
ApaI GGGCCC 1 cut(s) 812
ApeKI GCWGC 5 cut(s) 74, 92, 511, 1007, 1038
ArsI GACNNNNNNTTYG 2 cut(s) 1012, 1044
Asp700I GAANNNNTTC 1 cut(s) 888
AspLEI GCGC 2 cut(s) 145, 980
AspS9I GGNCC 3 cut(s) 808, 809, 834
AsuHPI GGTGA 3 cut(s) 279, 462, 479
BaeGI GKGCMC 2 cut(s) 812, 955
BanII GRGCYC 1 cut(s) 812
BbsI GAAGAC 1 cut(s) 736
BbvCI CCTCAGC 1 cut(s) 1045
BbvI GCAGC 5 cut(s) 86, 104, 523, 1019, 1050
BccI CCATC 4 cut(s) 352, 481, 622, 1046
BcgI CGANNNNNNTGC 2 cut(s) 81, 115
BfaI CTAG 1 cut(s) 125
BfmI CTRYAG 2 cut(s) 666, 981
BfoI RGCGCY 1 cut(s) 981
BisI GCNGC 5 cut(s) 75, 93, 512, 1008, 1039
BlsI GCNGC 5 cut(s) 76, 94, 513, 1009, 1040
BmcAI AGTACT 1 cut(s) 630
BmgT120I GGNCC 3 cut(s) 808, 809, 834
BmiI GGNNCC 2 cut(s) 6, 810
BmsI GCATC 1 cut(s) 282
BpiI GAAGAC 1 cut(s) 736
BpmI CTGGAG 2 cut(s) 447, 958
Bpu10I CCTNAGC 1 cut(s) 1045
BsaBI GATNNNNATC 1 cut(s) 503
BsaJI CCNNGG 3 cut(s) 42, 837, 877
BsaXI ACNNNNNCTCC 2 cut(s) 420, 450
Bsc4I CCNNNNNNNGG 1 cut(s) 831
Bse1I ACTGG 1 cut(s) 345
Bse3DI GCAATG 2 cut(s) 558, 1040
Bse8I GATNNNNATC 1 cut(s) 503
BseDI CCNNGG 3 cut(s) 42, 837, 877
BseGI GGATG 2 cut(s) 363, 473
BseJI GATNNNNATC 1 cut(s) 503
BseLI CCNNNNNNNGG 1 cut(s) 831
BseMI GCAATG 2 cut(s) 558, 1040
BseMII CTCAG 2 cut(s) 762, 1059
BseNI ACTGG 1 cut(s) 345
BseRI GAGGAG 2 cut(s) 391, 474
BseSI GKGCMC 2 cut(s) 812, 955
BseXI GCAGC 5 cut(s) 86, 104, 523, 1019, 1050
BsgI GTGCAG 2 cut(s) 74, 93
BshFI GGCC 4 cut(s) 234, 718, 810, 835
BslFI GGGAC 1 cut(s) 421
BslI CCNNNNNNNGG 1 cut(s) 831
BsmFI GGGAC 1 cut(s) 421
BsnI GGCC 4 cut(s) 234, 718, 810, 835
Bsp120I GGGCCC 1 cut(s) 808
Bsp1286I GDGCHC 2 cut(s) 812, 955
Bsp1407I TGTACA 1 cut(s) 11
Bsp143I GATC 4 cut(s) 455, 504, 672, 911
Bsp19I CCATGG 1 cut(s) 42
BspANI GGCC 4 cut(s) 234, 718, 810, 835
BspCNI CTCAG 2 cut(s) 761, 1058
BspHI TCATGA 1 cut(s) 918
BspLI GGNNCC 2 cut(s) 6, 810
BspPI GGATC 2 cut(s) 463, 919
BsrDI GCAATG 2 cut(s) 558, 1040
BsrGI TGTACA 1 cut(s) 11
BsrI ACTGG 1 cut(s) 345
BssECI CCNNGG 3 cut(s) 42, 837, 877
BssMI GATC 4 cut(s) 455, 504, 672, 911
BssT1I CCWWGG 2 cut(s) 42, 837
Bst4CI ACNGT 6 cut(s) 154, 295, 317, 611, 628, 670
Bst6I CTCTTC 1 cut(s) 638
BstAUI TGTACA 1 cut(s) 11
BstC8I GCNNGC 4 cut(s) 147, 549, 559, 797
BstDEI CTNAG 3 cut(s) 713, 748, 1045
BstDSI CCRYGG 1 cut(s) 42
BstF5I GGATG 2 cut(s) 363, 473
BstH2I RGCGCY 1 cut(s) 981
BstHHI GCGC 2 cut(s) 145, 980
BstKTI GATC 4 cut(s) 458, 507, 675, 914
BstMBI GATC 4 cut(s) 455, 504, 672, 911
BstNSI RCATGY 1 cut(s) 256
BstSFI CTRYAG 2 cut(s) 666, 981
BstSLI GKGCMC 2 cut(s) 812, 955
BstV1I GCAGC 5 cut(s) 86, 104, 523, 1019, 1050
BstV2I GAAGAC 1 cut(s) 736
BstX2I RGATCY 1 cut(s) 911
BstXI CCANNNNNNTGG 3 cut(s) 49, 403, 705
BstYI RGATCY 1 cut(s) 911
BsuRI GGCC 4 cut(s) 234, 718, 810, 835
BtgI CCRYGG 1 cut(s) 42
BtsCI GGATG 2 cut(s) 363, 473
BtsI GCAGTG 2 cut(s) 206, 907
BtsIMutI CAGTG 4 cut(s) 206, 426, 675, 907
Cac8I GCNNGC 4 cut(s) 147, 549, 559, 797
CaiI CAGNNNCTG 1 cut(s) 425
CciI TCATGA 1 cut(s) 918
CfoI GCGC 2 cut(s) 145, 980
Cfr13I GGNCC 3 cut(s) 808, 809, 834
Csp6I GTAC 4 cut(s) 12, 26, 629, 710
CviAII CATG 8 cut(s) 15, 43, 97, 253, 576, 826, 862, 919
CviQI GTAC 4 cut(s) 12, 26, 629, 710
DdeI CTNAG 3 cut(s) 713, 748, 1045
DpnI GATC 4 cut(s) 457, 506, 674, 913
DpnII GATC 4 cut(s) 455, 504, 672, 911
Eam1104I CTCTTC 1 cut(s) 638
EarI CTCTTC 1 cut(s) 638
Eco130I CCWWGG 2 cut(s) 42, 837
Eco147I AGGCCT 1 cut(s) 718
Eco24I GRGCYC 1 cut(s) 812
Eco47III AGCGCT 1 cut(s) 979
Eco57I CTGAAG 3 cut(s) 265, 285, 572
EcoO109I RGGNCCY 3 cut(s) 808, 809, 834
EcoT14I CCWWGG 2 cut(s) 42, 837
EcoT38I GRGCYC 1 cut(s) 812
ErhI CCWWGG 2 cut(s) 42, 837
FaeI CATG 8 cut(s) 18, 46, 100, 256, 579, 829, 865, 922
FaqI GGGAC 1 cut(s) 421
FatI CATG 8 cut(s) 14, 42, 96, 252, 575, 825, 861, 918
Fnu4HI GCNGC 5 cut(s) 75, 93, 512, 1008, 1039
FokI GGATG 2 cut(s) 370, 460
FriOI GRGCYC 1 cut(s) 812
Fsp4HI GCNGC 5 cut(s) 75, 93, 512, 1008, 1039
FspBI CTAG 1 cut(s) 125
GlaI GCGC 2 cut(s) 144, 979
GluI GCNGC 5 cut(s) 75, 93, 512, 1008, 1039
GsuI CTGGAG 2 cut(s) 447, 958
HaeII RGCGCY 1 cut(s) 981
HaeIII GGCC 4 cut(s) 234, 718, 810, 835
HhaI GCGC 2 cut(s) 145, 980
Hin1II CATG 8 cut(s) 18, 46, 100, 256, 579, 829, 865, 922
Hin6I GCGC 2 cut(s) 143, 978
HinP1I GCGC 2 cut(s) 143, 978
HinfI GANTC 1 cut(s) 763
HphI GGTGA 3 cut(s) 279, 462, 479
Hpy166II GTNNAC 2 cut(s) 133, 218
Hpy188I TCNGA 1 cut(s) 591
Hpy188III TCNNGA 3 cut(s) 355, 426, 919
Hpy8I GTNNAC 2 cut(s) 133, 218
HpyAV CCTTC 3 cut(s) 173, 236, 655
HpyCH4III ACNGT 6 cut(s) 154, 295, 317, 611, 628, 670
HpyCH4V TGCA 8 cut(s) 55, 74, 511, 551, 799, 926, 1033, 1038
HpyF3I CTNAG 3 cut(s) 713, 748, 1045
Hsp92II CATG 8 cut(s) 18, 46, 100, 256, 579, 829, 865, 922
HspAI GCGC 2 cut(s) 143, 978
Kzo9I GATC 4 cut(s) 455, 504, 672, 911
LmnI GCTCC 3 cut(s) 10, 428, 977
LpnPI CCDG 9 cut(s) 326, 340, 353, 411, 503, 732, 817, 825, 988
Lsp1109I GCAGC 5 cut(s) 86, 104, 523, 1019, 1050
LweI GCATC 1 cut(s) 282
MaeI CTAG 1 cut(s) 125
MaeIII GTNAC 1 cut(s) 652
MalI GATC 4 cut(s) 457, 506, 674, 913
MboI GATC 4 cut(s) 455, 504, 672, 911
MboII GAAGA 6 cut(s) 199, 578, 625, 628, 736, 865
MfeI CAATTG 2 cut(s) 597, 927
MflI RGATCY 1 cut(s) 911
MhlI GDGCHC 2 cut(s) 812, 955
MluCI AATT 5 cut(s) 221, 237, 324, 597, 927
MlyI GAGTC 1 cut(s) 772
MmeI TCCRAC 1 cut(s) 501
MnlI CCTC 7 cut(s) 369, 372, 495, 530, 887, 1035, 1054
MroXI GAANNNNTTC 1 cut(s) 888
MseI TTAA 2 cut(s) 603, 1057
MslI CAYNNNNRTG 2 cut(s) 47, 728
MunI CAATTG 2 cut(s) 597, 927
NcoI CCATGG 1 cut(s) 42
NdeII GATC 4 cut(s) 455, 504, 672, 911
NlaIII CATG 8 cut(s) 18, 46, 100, 256, 579, 829, 865, 922
NlaIV GGNNCC 2 cut(s) 6, 810
NmeAIII GCCGAG 1 cut(s) 858
NmuCI GTSAC 1 cut(s) 652
NspI RCATGY 1 cut(s) 256
PagI TCATGA 1 cut(s) 918
PceI AGGCCT 1 cut(s) 718
PciI ACATGT 1 cut(s) 252
PdmI GAANNNNTTC 1 cut(s) 888
PkrI GCNGC 5 cut(s) 76, 94, 513, 1009, 1040
PleI GAGTC 1 cut(s) 771
PpsI GAGTC 1 cut(s) 771
PscI ACATGT 1 cut(s) 252
PspN4I GGNNCC 2 cut(s) 6, 810
PspOMI GGGCCC 1 cut(s) 808
PspPI GGNCC 3 cut(s) 808, 809, 834
PsrI GAACNNNNNNTAC 1 cut(s) 36
PstNI CAGNNNCTG 1 cut(s) 425
PsuI RGATCY 1 cut(s) 911
RsaI GTAC 4 cut(s) 13, 27, 630, 711
RsaNI GTAC 4 cut(s) 12, 26, 629, 710
RseI CAYNNNNRTG 2 cut(s) 47, 728
SaqAI TTAA 2 cut(s) 603, 1057
SatI GCNGC 5 cut(s) 75, 93, 512, 1008, 1039
Sau3AI GATC 4 cut(s) 455, 504, 672, 911
Sau96I GGNCC 3 cut(s) 808, 809, 834
ScaI AGTACT 1 cut(s) 630
SchI GAGTC 1 cut(s) 772
SduI GDGCHC 2 cut(s) 812, 955
SfaNI GCATC 1 cut(s) 282
SfcI CTRYAG 2 cut(s) 666, 981
SmiMI CAYNNNNRTG 2 cut(s) 47, 728
Sse9I AATT 5 cut(s) 221, 237, 324, 597, 927
SseBI AGGCCT 1 cut(s) 718
SspI AATATT 1 cut(s) 583
SspMI CTAG 1 cut(s) 125
StuI AGGCCT 1 cut(s) 718
StyI CCWWGG 2 cut(s) 42, 837
TaaI ACNGT 6 cut(s) 154, 295, 317, 611, 628, 670
TaqI TCGA 1 cut(s) 101
TasI AATT 5 cut(s) 221, 237, 324, 597, 927
TatI WGTACW 3 cut(s) 11, 25, 628
Tru1I TTAA 2 cut(s) 603, 1057
Tru9I TTAA 2 cut(s) 603, 1057
TscAI CASTG 4 cut(s) 213, 426, 675, 907
TseFI GTSAC 1 cut(s) 652
TseI GCWGC 5 cut(s) 74, 92, 511, 1007, 1038
Tsp45I GTSAC 1 cut(s) 652
TspDTI ATGAA 5 cut(s) 49, 163, 325, 881, 1005
TspRI CASTG 4 cut(s) 213, 426, 675, 907
XceI RCATGY 1 cut(s) 256
XmnI GAANNNNTTC 1 cut(s) 888
XspI CTAG 1 cut(s) 125
ZrmI AGTACT 1 cut(s) 630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.