Rh2CG603700

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000534
Physical Location & Seq
Reverse (-)
77911043 .. 77913135
2093 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG603700.1

Sequence Viewer

Length: 810 bp
ATGCTATTGGCATATACTGGCATCAACTACAGCTCACCAACACTTGCTTCAGCTATGGGAAACGTCATTCCAATCTCTACTTTCATACTGGCAATCATTTTCAGGATGGAAAAGCTGGATTTGAGGAGGTCAAGCTGTCAAGCCAAAGTGTTAGGGACTATTGTATCAGTATCTGGAGCATTTATAGTAATTCTATACAAGGGATCAGTAATCTTTTCACCATCCAACTCTCCTGACCAGAACTTCATGATGACCTTGCAGCAGTCAAAGTGGGTCTTTGGAGGTCTTATGCTTGCAATGGCGAGCGTTTTGAAGGCATCATGGAATATTCTTCAGACATCAATTGTTAAGAACTATCCATCAATGATTACCGTACTCTTCTTCTACACCTTCTTTGTGACAATTCAATCCACAGTGTTCTCTCTATTTTTGGAAAGAAATCCAAATGCTTGGGTACTTAGGCCTGACATTGAGATGATCTCCATTGTCTTCTCAGCTATCTTCGGGAGTCTGTTCCATATTGGTGTTGAAACCTGGTGCTTGCACCAAAAGGGACCTATCTTTGTAGCCATGTTCAGGCCCTTGGGAGTTGCCATTGCTGCAGTCATGGTAGTTATCTTCCTTGGCGACGCACTTCACCTTGGCAGTGTGATTGGATCTATCATTATTGCAATTGGGTTTTATGCTATGATATGGGCACAAATCAAAGAAAATAGCACCAACGGTATAGCAAATGAAGTCCAAAGCTTGGCATCATCCACTCAAAAGACCCCTCTTTTGCAATGCAAAACCTCAGGAGAGGATGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

269

Amino Acids

29.47

Weight (kDa)

9.01

Isoelectric Point (pI)

38.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 94 - 230 1.4e-06 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000534)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g50400 FvH4_6g50410 FvH4_6g50410 FvH4_6g50410 FvH4_6g50410 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420
malus_domestica MD09G1036400.v1.1 MD09G1036600.v1.1 MD17G1038100.v1.1 MD17G1038200.v1.1
prunus_persica Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G281000_v2.0.a1 Prupe.3G281000_v2.0.a1 Prupe.3G281000_v2.0.a1 Prupe.3G281000_v2.0.a1
pyrus_communis pycom111g02920 pycom111g02930 pycom111g02940 pycom111g02950 pycom16g07600 pycom17g03430 pycom17g03440
rosa_chinensis RchiOBHm_Chr2g0170801 RchiOBHm_Chr2g0170811 RchiOBHm_Chr2g0170821 RchiOBHm_Chr2g0170831 RchiOBHm_Chr2g0170841
rosa_laevigata RLG00000021981 RLG00000021983 RLG00000021984 RLG00000021985
rosa_multiflora Rmu_co8418849.1_g000001 Rmu_co8437261.1_g000001 Rmu_sc0008393.1_g000003
rosa_roxburghii Rroxscaffold_1G00030380 Rroxscaffold_2G00081040 Rroxscaffold_2G00081050 Rroxscaffold_2G00081060
rosa_rugosa Rorug02G0551300 Rorug02G0551400 Rorug02G0551700 Rorug02G0551800
rosa_samantha Rh2AG624300 Rh2BG634700 Rh2BG634800 Rh2BG634900 Rh2BG635000 Rh2CG603500 Rh2CG603600 Rh2CG603700 Rh2CG603800
rosa_wichuraiana Rw0G017070 Rw2G051700 Rw2G051710 Rw2G051720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 748
AclWI GGATC 2 cut(s) 211, 664
AcuI CTGAAG 2 cut(s) 33, 317
AfaI GTAC 2 cut(s) 375, 456
AfiI CCNNNNNNNGG 2 cut(s) 576, 748
AgsI TTSAA 3 cut(s) 313, 407, 530
AjnI CCWGG 1 cut(s) 533
AjuI GAANNNNNNNTTGG 2 cut(s) 31, 63
AluBI AGCT 6 cut(s) 33, 53, 115, 135, 497, 747
AluI AGCT 6 cut(s) 33, 53, 115, 135, 497, 747
AlwI GGATC 2 cut(s) 211, 664
AlwNI CAGNNNCTG 1 cut(s) 173
AoxI GGCC 2 cut(s) 461, 578
ApeKI GCWGC 2 cut(s) 259, 599
ArsI GACNNNNNNTTYG 2 cut(s) 760, 792
AspS9I GGNCC 2 cut(s) 554, 579
AsuHPI GGTGA 3 cut(s) 27, 210, 629
AvaII GGWCC 1 cut(s) 554
AxyI CCTNAGG 1 cut(s) 793
BaeGI GKGCMC 1 cut(s) 700
BaeI ACNNNNGTAYC 2 cut(s) 147, 180
BbsI GAAGAC 1 cut(s) 481
BbvI GCAGC 2 cut(s) 271, 586
BccI CCATC 3 cut(s) 100, 229, 367
BciT130I CCWGG 1 cut(s) 535
BfmI CTRYAG 2 cut(s) 28, 600
BisI GCNGC 2 cut(s) 260, 600
BlsI GCNGC 2 cut(s) 261, 601
Bme1390I CCNGG 1 cut(s) 535
Bme18I GGWCC 1 cut(s) 554
BmgT120I GGNCC 2 cut(s) 554, 579
BmiI GGNNCC 1 cut(s) 555
BmrFI CCNGG 1 cut(s) 535
BmsI GCATC 3 cut(s) 30, 326, 761
BpiI GAAGAC 1 cut(s) 481
BplI GAGNNNNNCTC 2 cut(s) 464, 496
BpmI CTGGAG 1 cut(s) 195
BsaJI CCNNGG 3 cut(s) 582, 622, 640
BsaXI ACNNNNNCTCC 1 cut(s) 789
Bsc4I CCNNNNNNNGG 2 cut(s) 576, 748
Bse1I ACTGG 2 cut(s) 22, 93
Bse21I CCTNAGG 1 cut(s) 793
Bse3DI GCAATG 3 cut(s) 303, 594, 788
BseBI CCWGG 1 cut(s) 535
BseDI CCNNGG 3 cut(s) 582, 622, 640
BseGI GGATG 4 cut(s) 111, 221, 755, 808
BseLI CCNNNNNNNGG 2 cut(s) 576, 748
BseMI GCAATG 3 cut(s) 303, 594, 788
BseMII CTCAG 2 cut(s) 507, 807
BseNI ACTGG 2 cut(s) 22, 93
BseRI GAGGAG 1 cut(s) 139
BseSI GKGCMC 1 cut(s) 700
BseXI GCAGC 2 cut(s) 271, 586
BshFI GGCC 2 cut(s) 463, 580
BslFI GGGAC 2 cut(s) 169, 567
BslI CCNNNNNNNGG 2 cut(s) 576, 748
BsmFI GGGAC 2 cut(s) 169, 567
BsnI GGCC 2 cut(s) 463, 580
Bsp1286I GDGCHC 1 cut(s) 700
Bsp143I GATC 3 cut(s) 203, 477, 656
BspANI GGCC 2 cut(s) 463, 580
BspCNI CTCAG 2 cut(s) 506, 806
BspHI TCATGA 1 cut(s) 246
BspLI GGNNCC 1 cut(s) 555
BspMAI CTGCAG 1 cut(s) 604
BspPI GGATC 2 cut(s) 211, 664
BsrDI GCAATG 3 cut(s) 303, 594, 788
BsrI ACTGG 2 cut(s) 22, 93
BssECI CCNNGG 3 cut(s) 582, 622, 640
BssMI GATC 3 cut(s) 203, 477, 656
BssT1I CCWWGG 3 cut(s) 582, 622, 640
Bst2UI CCWGG 1 cut(s) 535
Bst4CI ACNGT 3 cut(s) 373, 415, 725
Bst6I CTCTTC 1 cut(s) 383
BstC8I GCNNGC 3 cut(s) 294, 304, 542
BstDEI CTNAG 3 cut(s) 458, 493, 793
BstF5I GGATG 4 cut(s) 111, 221, 755, 808
BstKTI GATC 3 cut(s) 206, 480, 659
BstMBI GATC 3 cut(s) 203, 477, 656
BstMWI GCNNNNNNNGC 1 cut(s) 599
BstNI CCWGG 1 cut(s) 535
BstSCI CCNGG 1 cut(s) 533
BstSFI CTRYAG 2 cut(s) 28, 600
BstSLI GKGCMC 1 cut(s) 700
BstV1I GCAGC 2 cut(s) 271, 586
BstV2I GAAGAC 1 cut(s) 481
BstX2I RGATCY 1 cut(s) 656
BstXI CCANNNNNNTGG 1 cut(s) 450
BstYI RGATCY 1 cut(s) 656
Bsu36I CCTNAGG 1 cut(s) 793
BsuRI GGCC 2 cut(s) 463, 580
BtsCI GGATG 4 cut(s) 111, 221, 755, 808
BtsI GCAGTG 1 cut(s) 652
BtsIMutI CAGTG 2 cut(s) 420, 652
Cac8I GCNNGC 3 cut(s) 294, 304, 542
CaiI CAGNNNCTG 1 cut(s) 173
CciI TCATGA 1 cut(s) 246
Cfr13I GGNCC 2 cut(s) 554, 579
CseI GACGC 1 cut(s) 638
CsiI ACCWGGT 1 cut(s) 533
Csp6I GTAC 2 cut(s) 374, 455
CviAII CATG 4 cut(s) 247, 321, 571, 607
CviQI GTAC 2 cut(s) 374, 455
DdeI CTNAG 3 cut(s) 458, 493, 793
DpnI GATC 3 cut(s) 205, 479, 658
DpnII GATC 3 cut(s) 203, 477, 656
Eam1104I CTCTTC 1 cut(s) 383
EarI CTCTTC 1 cut(s) 383
Eco130I CCWWGG 3 cut(s) 582, 622, 640
Eco147I AGGCCT 1 cut(s) 463
Eco47I GGWCC 1 cut(s) 554
Eco57I CTGAAG 2 cut(s) 33, 317
Eco81I CCTNAGG 1 cut(s) 793
EcoO109I RGGNCCY 2 cut(s) 554, 579
EcoRII CCWGG 1 cut(s) 533
EcoT14I CCWWGG 3 cut(s) 582, 622, 640
ErhI CCWWGG 3 cut(s) 582, 622, 640
FaeI CATG 4 cut(s) 250, 324, 574, 610
FalI AAGNNNNNCTT 2 cut(s) 260, 292
FaqI GGGAC 2 cut(s) 169, 567
FatI CATG 4 cut(s) 246, 320, 570, 606
Fnu4HI GCNGC 2 cut(s) 260, 600
FokI GGATG 3 cut(s) 118, 208, 742
Fsp4HI GCNGC 2 cut(s) 260, 600
GluI GCNGC 2 cut(s) 260, 600
GsuI CTGGAG 1 cut(s) 195
HaeIII GGCC 2 cut(s) 463, 580
HgaI GACGC 1 cut(s) 638
Hin1II CATG 4 cut(s) 250, 324, 574, 610
HindIII AAGCTT 1 cut(s) 745
HinfI GANTC 1 cut(s) 508
HphI GGTGA 3 cut(s) 27, 210, 629
Hpy188I TCNGA 1 cut(s) 336
Hpy188III TCNNGA 6 cut(s) 103, 174, 233, 247, 505, 795
Hpy99I CGWCG 1 cut(s) 632
HpyAV CCTTC 2 cut(s) 307, 400
HpyCH4III ACNGT 3 cut(s) 373, 415, 725
HpyCH4IV ACGT 1 cut(s) 63
HpyCH4V TGCA 7 cut(s) 259, 296, 544, 602, 671, 781, 786
HpyF10VI GCNNNNNNNGC 1 cut(s) 599
HpyF3I CTNAG 3 cut(s) 458, 493, 793
HpySE526I ACGT 1 cut(s) 63
Hsp92II CATG 4 cut(s) 250, 324, 574, 610
Kzo9I GATC 3 cut(s) 203, 477, 656
LmnI GCTCC 1 cut(s) 176
Lsp1109I GCAGC 2 cut(s) 271, 586
LweI GCATC 3 cut(s) 30, 326, 761
MabI ACCWGGT 1 cut(s) 533
MaeII ACGT 1 cut(s) 63
MaeIII GTNAC 1 cut(s) 397
MalI GATC 3 cut(s) 205, 479, 658
MboI GATC 3 cut(s) 203, 477, 656
MboII GAAGA 6 cut(s) 323, 370, 373, 481, 493, 610
MfeI CAATTG 2 cut(s) 342, 672
MflI RGATCY 1 cut(s) 656
MhlI GDGCHC 1 cut(s) 700
MluCI AATT 4 cut(s) 189, 342, 402, 672
MlyI GAGTC 1 cut(s) 517
MmeI TCCRAC 1 cut(s) 249
MnlI CCTC 6 cut(s) 117, 120, 275, 783, 793, 802
MseI TTAA 1 cut(s) 348
MslI CAYNNNNRTG 2 cut(s) 473, 522
MspR9I CCNGG 1 cut(s) 535
MunI CAATTG 2 cut(s) 342, 672
MvaI CCWGG 1 cut(s) 535
MwoI GCNNNNNNNGC 1 cut(s) 599
NdeII GATC 3 cut(s) 203, 477, 656
NlaIII CATG 4 cut(s) 250, 324, 574, 610
NlaIV GGNNCC 1 cut(s) 555
NmuCI GTSAC 1 cut(s) 397
PagI TCATGA 1 cut(s) 246
PceI AGGCCT 1 cut(s) 463
PflMI CCANNNNNTGG 1 cut(s) 748
PkrI GCNGC 2 cut(s) 261, 601
PleI GAGTC 1 cut(s) 516
PpsI GAGTC 1 cut(s) 516
PpuMI RGGWCCY 1 cut(s) 554
Psp5II RGGWCCY 1 cut(s) 554
Psp6I CCWGG 1 cut(s) 533
PspGI CCWGG 1 cut(s) 533
PspN4I GGNNCC 1 cut(s) 555
PspPI GGNCC 2 cut(s) 554, 579
PspPPI RGGWCCY 1 cut(s) 554
PstI CTGCAG 1 cut(s) 604
PstNI CAGNNNCTG 1 cut(s) 173
PsuI RGATCY 1 cut(s) 656
RsaI GTAC 2 cut(s) 375, 456
RsaNI GTAC 2 cut(s) 374, 455
RseI CAYNNNNRTG 2 cut(s) 473, 522
SaqAI TTAA 1 cut(s) 348
SatI GCNGC 2 cut(s) 260, 600
Sau3AI GATC 3 cut(s) 203, 477, 656
Sau96I GGNCC 2 cut(s) 554, 579
SchI GAGTC 1 cut(s) 517
ScrFI CCNGG 1 cut(s) 535
SduI GDGCHC 1 cut(s) 700
SexAI ACCWGGT 1 cut(s) 533
SfaNI GCATC 3 cut(s) 30, 326, 761
SfcI CTRYAG 2 cut(s) 28, 600
SinI GGWCC 1 cut(s) 554
SmiMI CAYNNNNRTG 2 cut(s) 473, 522
Sse9I AATT 4 cut(s) 189, 342, 402, 672
SseBI AGGCCT 1 cut(s) 463
SspI AATATT 1 cut(s) 328
StuI AGGCCT 1 cut(s) 463
StyD4I CCNGG 1 cut(s) 533
StyI CCWWGG 3 cut(s) 582, 622, 640
TaaI ACNGT 3 cut(s) 373, 415, 725
TaiI ACGT 1 cut(s) 66
TasI AATT 4 cut(s) 189, 342, 402, 672
Tru1I TTAA 1 cut(s) 348
Tru9I TTAA 1 cut(s) 348
TscAI CASTG 2 cut(s) 420, 652
TseFI GTSAC 1 cut(s) 397
TseI GCWGC 2 cut(s) 259, 599
Tsp45I GTSAC 1 cut(s) 397
TspDTI ATGAA 3 cut(s) 73, 235, 750
TspRI CASTG 2 cut(s) 420, 652
Van91I CCANNNNNTGG 1 cut(s) 748
VpaK11BI GGWCC 1 cut(s) 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.