RLG00000021985

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000534
Physical Location & Seq
Reverse (-)
79739450 .. 79741476
2027 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021985

Sequence Viewer

Length: 801 bp
ATGGTGTCTTGTACATGGAGTGATGTGCTACCTTTTGCAGCTATGGTAATGGTGCAGTTCACAGACATAGGTGCAGCAACTATAAGCAAAGCAGCCATGACGAAAGGAGTGAGCAGTTATGTTTTAGTTGCTTACTCCAATGCGCTTGGTACCGTCTTTCTTCTTCCTTGTTTCATCTTACAAAATAAGGTTTCTCTTACCTTTTCACTCCTTGGTGGACAATTCCTTCTGGGCATGATTGGGAGTTCAAGCTTGTTATTGGCATATAGTGGCATCAACTACAGCTCACCAACACTTGCTTCAGCTATGGCAAATCTTATACCAATCTTTACATTCATACTGGCAGTCATTTTCAGGATGGAAAAGCTAGATTTGAGGAGGTCAAGCTGCCAAGCCAAAGTGTTAGGGACTATAGTATCAGTATCTGGAGCATTTGTAGTAATTCTATACAAGGGATCAGTGATCTTTTCACCATCCAACTCTCCTGACCAGAACTTCATGATGATCTCACAGCAGTCAAAGTGGGTCTTTGGAGGTCTTATGCTGGCAATGGTGTGCCTTCTGAACGCAACATGGGGTATTCTTCAGACATCATTTGTTCAGAACTGTCCATCAATGATTACCATACTCTTCTTCTACAACTTCTTTGTCACAATCCAGTGCACAATATTCTCTCTAATTATGGAAAGGACTTGGAATGCTTGGGTAATAAGGCCTGACATTGAGATGATCTCCATTGTCTTCTCAGTAAGAAGACCGACCGATAGACCTTTCATTCAGTTGCTATTTCAGTATCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

267

Amino Acids

29.47

Weight (kDa)

9.11

Isoelectric Point (pI)

42.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 27 - 148 3e-10 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000534)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g50400 FvH4_6g50410 FvH4_6g50410 FvH4_6g50410 FvH4_6g50410 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420
malus_domestica MD09G1036400.v1.1 MD09G1036600.v1.1 MD17G1038100.v1.1 MD17G1038200.v1.1
prunus_persica Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G281000_v2.0.a1 Prupe.3G281000_v2.0.a1 Prupe.3G281000_v2.0.a1 Prupe.3G281000_v2.0.a1
pyrus_communis pycom111g02920 pycom111g02930 pycom111g02940 pycom111g02950 pycom16g07600 pycom17g03430 pycom17g03440
rosa_chinensis RchiOBHm_Chr2g0170801 RchiOBHm_Chr2g0170811 RchiOBHm_Chr2g0170821 RchiOBHm_Chr2g0170831 RchiOBHm_Chr2g0170841
rosa_laevigata RLG00000021981 RLG00000021983 RLG00000021984 RLG00000021985
rosa_multiflora Rmu_co8418849.1_g000001 Rmu_co8437261.1_g000001 Rmu_sc0008393.1_g000003
rosa_roxburghii Rroxscaffold_1G00030380 Rroxscaffold_2G00081040 Rroxscaffold_2G00081050 Rroxscaffold_2G00081060
rosa_rugosa Rorug02G0551300 Rorug02G0551400 Rorug02G0551700 Rorug02G0551800
rosa_samantha Rh2AG624300 Rh2BG634700 Rh2BG634800 Rh2BG634900 Rh2BG635000 Rh2CG603500 Rh2CG603600 Rh2CG603700 Rh2CG603800
rosa_wichuraiana Rw0G017070 Rw2G051700 Rw2G051710 Rw2G051720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 149
AccB1I GGYRCC 1 cut(s) 149
AclWI GGATC 1 cut(s) 463
AcuI CTGAAG 2 cut(s) 285, 569
AfaI GTAC 2 cut(s) 13, 151
AgsI TTSAA 1 cut(s) 249
AjuI GAANNNNNNNTTGG 2 cut(s) 283, 315
AluBI AGCT 6 cut(s) 41, 252, 285, 305, 367, 387
AluI AGCT 6 cut(s) 41, 252, 285, 305, 367, 387
Alw21I GWGCWC 1 cut(s) 665
Alw44I GTGCAC 1 cut(s) 661
AlwI GGATC 1 cut(s) 463
AlwNI CAGNNNCTG 1 cut(s) 425
AoxI GGCC 1 cut(s) 713
ApaLI GTGCAC 1 cut(s) 661
ApeKI GCWGC 4 cut(s) 38, 74, 92, 387
Asp718I GGTACC 1 cut(s) 149
AspLEI GCGC 1 cut(s) 145
AsuHPI GGTGA 2 cut(s) 279, 462
BaeGI GKGCMC 1 cut(s) 665
BaeI ACNNNNGTAYC 2 cut(s) 399, 432
BanI GGYRCC 1 cut(s) 149
BbsI GAAGAC 2 cut(s) 733, 760
Bbv12I GWGCWC 1 cut(s) 665
BbvI GCAGC 4 cut(s) 50, 86, 104, 374
BccI CCATC 3 cut(s) 352, 481, 619
BfaI CTAG 1 cut(s) 368
BfmI CTRYAG 2 cut(s) 280, 411
BisI GCNGC 4 cut(s) 39, 75, 93, 388
BlsI GCNGC 4 cut(s) 40, 76, 94, 389
BmiI GGNNCC 1 cut(s) 151
BmsI GCATC 1 cut(s) 282
BpiI GAAGAC 2 cut(s) 733, 760
BplI GAGNNNNNCTC 2 cut(s) 716, 748
BpmI CTGGAG 1 cut(s) 447
BsaJI CCNNGG 1 cut(s) 211
BsaXI ACNNNNNCTCC 2 cut(s) 420, 450
Bse1I ACTGG 2 cut(s) 345, 658
Bse3DI GCAATG 1 cut(s) 555
BseDI CCNNGG 1 cut(s) 211
BseGI GGATG 2 cut(s) 363, 473
BseMI GCAATG 1 cut(s) 555
BseMII CTCAG 1 cut(s) 759
BseNI ACTGG 2 cut(s) 345, 658
BseRI GAGGAG 1 cut(s) 391
BseSI GKGCMC 1 cut(s) 665
BseXI GCAGC 4 cut(s) 50, 86, 104, 374
BsgI GTGCAG 2 cut(s) 74, 93
Bsh1285I CGRYCG 1 cut(s) 762
BshFI GGCC 1 cut(s) 715
BshNI GGYRCC 1 cut(s) 149
BsiEI CGRYCG 1 cut(s) 762
BsiHKAI GWGCWC 1 cut(s) 665
BslFI GGGAC 1 cut(s) 421
BsmFI GGGAC 1 cut(s) 421
BsmI GAATGC 1 cut(s) 703
BsnI GGCC 1 cut(s) 715
Bsp1286I GDGCHC 1 cut(s) 665
Bsp1407I TGTACA 1 cut(s) 11
Bsp143I GATC 4 cut(s) 455, 462, 504, 729
BspANI GGCC 1 cut(s) 715
BspCNI CTCAG 1 cut(s) 758
BspHI TCATGA 1 cut(s) 498
BspLI GGNNCC 1 cut(s) 151
BspPI GGATC 1 cut(s) 463
BspT107I GGYRCC 1 cut(s) 149
BsrDI GCAATG 1 cut(s) 555
BsrGI TGTACA 1 cut(s) 11
BsrI ACTGG 2 cut(s) 345, 658
BssECI CCNNGG 1 cut(s) 211
BssMI GATC 4 cut(s) 455, 462, 504, 729
BssT1I CCWWGG 1 cut(s) 211
Bst4CI ACNGT 2 cut(s) 154, 608
Bst6I CTCTTC 1 cut(s) 635
BstAUI TGTACA 1 cut(s) 11
BstC8I GCNNGC 1 cut(s) 546
BstDEI CTNAG 1 cut(s) 745
BstF5I GGATG 2 cut(s) 363, 473
BstHHI GCGC 1 cut(s) 145
BstKTI GATC 4 cut(s) 458, 465, 507, 732
BstMBI GATC 4 cut(s) 455, 462, 504, 729
BstMCI CGRYCG 1 cut(s) 762
BstSFI CTRYAG 2 cut(s) 280, 411
BstSLI GKGCMC 1 cut(s) 665
BstV1I GCAGC 4 cut(s) 50, 86, 104, 374
BstV2I GAAGAC 2 cut(s) 733, 760
BsuRI GGCC 1 cut(s) 715
BtsCI GGATG 2 cut(s) 363, 473
BtsIMutI CAGTG 2 cut(s) 465, 665
Cac8I GCNNGC 1 cut(s) 546
CaiI CAGNNNCTG 1 cut(s) 425
CciI TCATGA 1 cut(s) 498
CfoI GCGC 1 cut(s) 145
Csp6I GTAC 2 cut(s) 12, 150
CviAII CATG 5 cut(s) 15, 97, 235, 499, 573
CviJI RGCY 9 cut(s) 41, 95, 252, 285, 305, 367, 387, 395, 715
CviKI_1 RGCY 9 cut(s) 41, 95, 252, 285, 305, 367, 387, 395, 715
CviQI GTAC 2 cut(s) 12, 150
DdeI CTNAG 1 cut(s) 745
DpnI GATC 4 cut(s) 457, 464, 506, 731
DpnII GATC 4 cut(s) 455, 462, 504, 729
Eam1104I CTCTTC 1 cut(s) 635
EarI CTCTTC 1 cut(s) 635
Eco130I CCWWGG 1 cut(s) 211
Eco147I AGGCCT 1 cut(s) 715
Eco57I CTGAAG 2 cut(s) 285, 569
EcoT14I CCWWGG 1 cut(s) 211
ErhI CCWWGG 1 cut(s) 211
FaeI CATG 5 cut(s) 18, 100, 238, 502, 576
FalI AAGNNNNNCTT 2 cut(s) 512, 544
FaqI GGGAC 1 cut(s) 421
FatI CATG 5 cut(s) 14, 96, 234, 498, 572
Fnu4HI GCNGC 4 cut(s) 39, 75, 93, 388
FokI GGATG 2 cut(s) 370, 460
Fsp4HI GCNGC 4 cut(s) 39, 75, 93, 388
FspBI CTAG 1 cut(s) 368
GlaI GCGC 1 cut(s) 144
GluI GCNGC 4 cut(s) 39, 75, 93, 388
GsuI CTGGAG 1 cut(s) 447
HaeIII GGCC 1 cut(s) 715
HhaI GCGC 1 cut(s) 145
Hin1II CATG 5 cut(s) 18, 100, 238, 502, 576
Hin6I GCGC 1 cut(s) 143
HinP1I GCGC 1 cut(s) 143
HindIII AAGCTT 1 cut(s) 250
HphI GGTGA 2 cut(s) 279, 462
Hpy166II GTNNAC 3 cut(s) 60, 218, 663
Hpy188I TCNGA 3 cut(s) 564, 588, 603
Hpy188III TCNNGA 4 cut(s) 355, 426, 485, 499
Hpy8I GTNNAC 3 cut(s) 60, 218, 663
HpyAV CCTTC 2 cut(s) 236, 569
HpyCH4III ACNGT 2 cut(s) 154, 608
HpyCH4V TGCA 4 cut(s) 38, 55, 74, 663
HpyF3I CTNAG 1 cut(s) 745
Hsp92II CATG 5 cut(s) 18, 100, 238, 502, 576
HspAI GCGC 1 cut(s) 143
KpnI GGTACC 1 cut(s) 153
Kzo9I GATC 4 cut(s) 455, 462, 504, 729
LmnI GCTCC 1 cut(s) 428
LpnPI CCDG 9 cut(s) 215, 326, 340, 411, 498, 503, 530, 671, 729
Lsp1109I GCAGC 4 cut(s) 50, 86, 104, 374
LweI GCATC 1 cut(s) 282
MaeI CTAG 1 cut(s) 368
MaeIII GTNAC 1 cut(s) 649
MalI GATC 4 cut(s) 457, 464, 506, 731
MboI GATC 4 cut(s) 455, 462, 504, 729
MboII GAAGA 7 cut(s) 152, 155, 575, 622, 625, 733, 765
MhlI GDGCHC 1 cut(s) 665
MluCI AATT 3 cut(s) 221, 441, 678
MmeI TCCRAC 1 cut(s) 501
MnlI CCTC 3 cut(s) 369, 372, 527
MslI CAYNNNNRTG 1 cut(s) 725
Mva1269I GAATGC 1 cut(s) 703
NdeII GATC 4 cut(s) 455, 462, 504, 729
NlaIII CATG 5 cut(s) 18, 100, 238, 502, 576
NlaIV GGNNCC 1 cut(s) 151
NmuCI GTSAC 1 cut(s) 649
PagI TCATGA 1 cut(s) 498
PceI AGGCCT 1 cut(s) 715
PctI GAATGC 1 cut(s) 703
PkrI GCNGC 4 cut(s) 40, 76, 94, 389
PspN4I GGNNCC 1 cut(s) 151
PstNI CAGNNNCTG 1 cut(s) 425
RsaI GTAC 2 cut(s) 13, 151
RsaNI GTAC 2 cut(s) 12, 150
RseI CAYNNNNRTG 1 cut(s) 725
SatI GCNGC 4 cut(s) 39, 75, 93, 388
Sau3AI GATC 4 cut(s) 455, 462, 504, 729
SduI GDGCHC 1 cut(s) 665
SfaNI GCATC 1 cut(s) 282
SfcI CTRYAG 2 cut(s) 280, 411
SmiMI CAYNNNNRTG 1 cut(s) 725
Sse9I AATT 3 cut(s) 221, 441, 678
SseBI AGGCCT 1 cut(s) 715
SspI AATATT 1 cut(s) 669
SspMI CTAG 1 cut(s) 368
StuI AGGCCT 1 cut(s) 715
StyI CCWWGG 1 cut(s) 211
TaaI ACNGT 2 cut(s) 154, 608
TaqII GACCGA 2 cut(s) 772, 776
TasI AATT 3 cut(s) 221, 441, 678
TatI WGTACW 1 cut(s) 11
TscAI CASTG 2 cut(s) 465, 665
TseFI GTSAC 1 cut(s) 649
TseI GCWGC 4 cut(s) 38, 74, 92, 387
Tsp45I GTSAC 1 cut(s) 649
TspDTI ATGAA 4 cut(s) 163, 325, 487, 763
TspRI CASTG 2 cut(s) 465, 665
VneI GTGCAC 1 cut(s) 661
XspI CTAG 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.