Rorug02G0551700

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000534
Physical Location & Seq
Forward (+)
67886251 .. 67892009
5759 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0551700.1

Sequence Viewer

Length: 1689 bp
ATGAAGATAAGATGTCAGAGATTCAAGAGCACCACTTTTCTCACTCTCCCTCTTCCAAAGATCGAGAGCATGGCAGCGACGGTGGCCGTCCCCAGTGCTATAGTCCATAAAGTTTTAACCACAAATAACTATGAAGATTGGAGTATTCGGGTCAAAACCTATCTGCTGGCTAAGGGTCTTTGGAAGGTCGTCGGATCCGCCATGGAACCGCCTAGAGTAGAAGATGAGGCTGAATTTGAGGCATGGAGTAAGAACAATGCAGAGGCTTTGCATGCTATTCATATTTCTTGCGGAGATGATACCTTCTCTTTTATCCGGGACATGGACACAGCTAAAGCTGCGTGGAATACTTTAGAAAAAAAATTGAAGCCCGCTAGACTACCAAAGAGGAAAGATTTTTCCGCTTATTGTTTAGATGTGAGATCTGACCATGAGTCCCCAATGGATGGTGAGCTACAACTAAAAGACCTTAATGAACCGGTAGTTGAAGGACACAGCAGCAATGCCAATTCCGCCACCAATGATCATGATGACAAAGCCTATAAACATTTCTTCGATTGTGTGAAGAATGGTTGGTGTGAGAGAAGCACAGCTGATGCACGGGAGTTTCTTAAAAAATATCCACACCCAATAAGAGAACTAAGACATCCATCATCCGGCCTTCCGGCTCTTCACTTCGCAATCTCGTGGTGGAACATGGTCATTGTAAAAGAGTTGGTGCAATTAATGACAGAGGAAGACTTGGAAGAAATATTGGACCCTTCTGGTCGTACAGCTATTTGCTCTGCTTTTGACTTTCCAGATCGACGCTCGGAGGAAATTGTTAAATGCTTGGTTGAAAAGAACAAAAAATTACTTAGCATTATTGATCCTTCCACCAAAATGATTCCACTTGTCAGGGCTCATCAAACTAAAGGGAACGGGGATCCCGTGGTTAAGTATCTGTATTCTGTCACTCCACTCGAAACTCTGATCAATGCTCACCAAGCAGCTGAAATTATTTCTCAAGGTTTTATAAATAAACAATTTGATATTTCTTGGGATTTAATACAACGCTGCCCAAGTTTGGCTATTACTAAAGACTTATCTGGAAAATATCCTTTGAATAGATTGGCTGAGAGCATGAAATGTAAACATTTATTTTTAAGCGAATGCGGACGCAACTTCTTGGAACGATGGATTTATGACCATACGGTCATACCACCTACCCCAACAAGAAACGATAGTAGTAGTATAAATGTTCATGATGCAGAAAATGGTAAATGTAATCAAAAGCGTCTCATTCACTCAGTTACCAGTTTATTCAGACGAATATTCGGAGGACTTTTGGGAATCAATCGCATACATCGTATGAAATTGATACATACCCAAATTCACGAATTTTTACATTGCATGTGTAAAGTGATTGAAACTAAAGATGTTAGACAAATGCAAGATGATATCCTTCTGCAATCAGCCATCTTCCGAGCAGTAGAACAAGGAAAAGTTGAGTTCATTACTCATATGGGTAAAGCAAATCGAGACATCTATCAGATTACTAATGAACAAGACATGACCGTGTTTCAATTTGCTGCTAATTGTCGTCAAGAAAAGATATATAGCCTTGTATATTTATATGGTAGCTACAGCAGAAATGAGATTTTTGGTAGGAGGCTAGGGATAAGTTTGACAATAATATGCTCCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

562

Amino Acids

64.36

Weight (kDa)

8.24

Isoelectric Point (pI)

40.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4219 PF13961 39 - 64 9.5e-08 Domain of unknown function (DUF4219)
Retrotran_gag_2 PF14223 52 - 121 1.1e-09 gag-polypeptide of LTR copia-type
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000534)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g50400 FvH4_6g50410 FvH4_6g50410 FvH4_6g50410 FvH4_6g50410 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420
malus_domestica MD09G1036400.v1.1 MD09G1036600.v1.1 MD17G1038100.v1.1 MD17G1038200.v1.1
prunus_persica Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G281000_v2.0.a1 Prupe.3G281000_v2.0.a1 Prupe.3G281000_v2.0.a1 Prupe.3G281000_v2.0.a1
pyrus_communis pycom111g02920 pycom111g02930 pycom111g02940 pycom111g02950 pycom16g07600 pycom17g03430 pycom17g03440
rosa_chinensis RchiOBHm_Chr2g0170801 RchiOBHm_Chr2g0170811 RchiOBHm_Chr2g0170821 RchiOBHm_Chr2g0170831 RchiOBHm_Chr2g0170841
rosa_laevigata RLG00000021981 RLG00000021983 RLG00000021984 RLG00000021985
rosa_multiflora Rmu_co8418849.1_g000001 Rmu_co8437261.1_g000001 Rmu_sc0008393.1_g000003
rosa_roxburghii Rroxscaffold_1G00030380 Rroxscaffold_2G00081040 Rroxscaffold_2G00081050 Rroxscaffold_2G00081060
rosa_rugosa Rorug02G0551300 Rorug02G0551400 Rorug02G0551700 Rorug02G0551800
rosa_samantha Rh2AG624300 Rh2BG634700 Rh2BG634800 Rh2BG634900 Rh2BG635000 Rh2CG603500 Rh2CG603600 Rh2CG603700 Rh2CG603800
rosa_wichuraiana Rw0G017070 Rw2G051700 Rw2G051710 Rw2G051720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1016
AasI GACNNNNNNGTC 1 cut(s) 1193
AccB7I CCANNNNNTGG 1 cut(s) 446
AciI CCGC 7 cut(s) 198, 209, 291, 372, 402, 513, 1155
AclWI GGATC 5 cut(s) 189, 202, 863, 920, 933
AcoI YGGCCR 1 cut(s) 84
AcsI RAATTY 3 cut(s) 233, 1371, 1379
AfaI GTAC 1 cut(s) 772
AfiI CCNNNNNNNGG 4 cut(s) 322, 446, 656, 1066
AgeI ACCGGT 1 cut(s) 478
AgsI TTSAA 7 cut(s) 25, 367, 488, 839, 1105, 1409, 1565
AhdI GACNNNNNGTC 1 cut(s) 433
AluBI AGCT 7 cut(s) 332, 338, 454, 593, 776, 992, 1623
AluI AGCT 7 cut(s) 332, 338, 454, 593, 776, 992, 1623
Alw21I GWGCWC 1 cut(s) 32
Alw26I GTCTC 2 cut(s) 1283, 1515
AlwI GGATC 5 cut(s) 189, 202, 863, 920, 933
AoxI GGCC 2 cut(s) 84, 658
ApeKI GCWGC 6 cut(s) 74, 338, 498, 989, 1056, 1571
ApoI RAATTY 3 cut(s) 233, 1371, 1379
AseI ATTAAT 1 cut(s) 725
AsiGI ACCGGT 1 cut(s) 478
AspS9I GGNCC 1 cut(s) 757
AsuC2I CCSGG 1 cut(s) 317
AsuHPI GGTGA 2 cut(s) 461, 974
AvaII GGWCC 1 cut(s) 757
BamHI GGATCC 2 cut(s) 194, 925
BanII GRGCYC 1 cut(s) 904
BauI CACGAG 1 cut(s) 685
BbsI GAAGAC 1 cut(s) 744
Bbv12I GWGCWC 1 cut(s) 32
BbvI GCAGC 6 cut(s) 86, 325, 510, 1001, 1043, 1558
BccI CCATC 4 cut(s) 440, 658, 1170, 1466
BceAI ACGGC 1 cut(s) 71
BclI TGATCA 2 cut(s) 523, 972
BcnI CCSGG 1 cut(s) 317
BcoDI GTCTC 2 cut(s) 1283, 1515
BfaI CTAG 3 cut(s) 213, 375, 1655
BfmI CTRYAG 2 cut(s) 99, 1624
BglII AGATCT 1 cut(s) 422
BisI GCNGC 6 cut(s) 75, 339, 499, 990, 1057, 1572
BlsI GCNGC 6 cut(s) 76, 340, 500, 991, 1058, 1573
Bme1390I CCNGG 1 cut(s) 317
Bme18I GGWCC 1 cut(s) 757
BmeRI GACNNNNNGTC 1 cut(s) 433
BmgT120I GGNCC 1 cut(s) 757
BmiI GGNNCC 4 cut(s) 196, 207, 759, 927
BmrFI CCNGG 1 cut(s) 317
BmrI ACTGGG 1 cut(s) 87
BmsI GCATC 2 cut(s) 586, 1237
BmuI ACTGGG 1 cut(s) 87
BpiI GAAGAC 1 cut(s) 744
Bpu10I CCTNAGC 1 cut(s) 171
BpuEI CTTGAG 1 cut(s) 990
BpuMI CCSGG 1 cut(s) 317
BsaJI CCNNGG 2 cut(s) 201, 930
BsaWI WCCGGW 1 cut(s) 478
Bsc4I CCNNNNNNNGG 4 cut(s) 322, 446, 656, 1066
Bse118I RCCGGY 1 cut(s) 478
Bse1I ACTGG 2 cut(s) 93, 1296
Bse3DI GCAATG 2 cut(s) 508, 1387
BseDI CCNNGG 2 cut(s) 201, 930
BseGI GGATG 3 cut(s) 451, 646, 653
BseLI CCNNNNNNNGG 4 cut(s) 322, 446, 656, 1066
BseMI GCAATG 2 cut(s) 508, 1387
BseMII CTCAG 2 cut(s) 1107, 1302
BseNI ACTGG 2 cut(s) 93, 1296
BseXI GCAGC 6 cut(s) 86, 325, 510, 1001, 1043, 1558
BshFI GGCC 2 cut(s) 86, 660
BshTI ACCGGT 1 cut(s) 478
BsiHKAI GWGCWC 1 cut(s) 32
BsiSI CCGG 4 cut(s) 316, 479, 657, 665
BslFI GGGAC 3 cut(s) 74, 332, 421
BslI CCNNNNNNNGG 4 cut(s) 322, 446, 656, 1066
BsmAI GTCTC 2 cut(s) 1283, 1515
BsmBI CGTCTC 1 cut(s) 1283
BsmFI GGGAC 3 cut(s) 74, 332, 421
BsmI GAATGC 1 cut(s) 1157
BsnI GGCC 2 cut(s) 86, 660
Bsp1286I GDGCHC 2 cut(s) 32, 904
Bsp143I GATC 8 cut(s) 60, 194, 422, 523, 802, 868, 925, 972
Bsp19I CCATGG 1 cut(s) 201
BspACI CCGC 7 cut(s) 198, 209, 291, 372, 402, 513, 1155
BspANI GGCC 2 cut(s) 86, 660
BspCNI CTCAG 2 cut(s) 1108, 1301
BspHI TCATGA 2 cut(s) 526, 1243
BspLI GGNNCC 4 cut(s) 196, 207, 759, 927
BspPI GGATC 5 cut(s) 189, 202, 863, 920, 933
BspQI GCTCTTC 1 cut(s) 675
BsrDI GCAATG 2 cut(s) 508, 1387
BsrFI RCCGGY 1 cut(s) 478
BsrI ACTGG 2 cut(s) 93, 1296
BssAI RCCGGY 1 cut(s) 478
BssECI CCNNGG 2 cut(s) 201, 930
BssMI GATC 8 cut(s) 60, 194, 422, 523, 802, 868, 925, 972
BssSI CACGAG 1 cut(s) 685
BssT1I CCWWGG 1 cut(s) 201
Bst2BI CACGAG 1 cut(s) 685
Bst4CI ACNGT 3 cut(s) 82, 1195, 1558
Bst6I CTCTTC 2 cut(s) 57, 675
BstC8I GCNNGC 3 cut(s) 168, 273, 372
BstDEI CTNAG 5 cut(s) 171, 641, 857, 1116, 1288
BstDSI CCRYGG 2 cut(s) 201, 930
BstF5I GGATG 3 cut(s) 451, 646, 653
BstKTI GATC 8 cut(s) 63, 197, 425, 526, 805, 871, 928, 975
BstMAI GTCTC 2 cut(s) 1283, 1515
BstMBI GATC 8 cut(s) 60, 194, 422, 523, 802, 868, 925, 972
BstMWI GCNNNNNNNGC 5 cut(s) 83, 272, 338, 512, 986
BstNSI RCATGY 2 cut(s) 275, 1396
BstSCI CCNGG 1 cut(s) 315
BstSFI CTRYAG 2 cut(s) 99, 1624
BstV1I GCAGC 6 cut(s) 86, 325, 510, 1001, 1043, 1558
BstV2I GAAGAC 1 cut(s) 744
BstX2I RGATCY 3 cut(s) 194, 422, 925
BstYI RGATCY 3 cut(s) 194, 422, 925
BsuRI GGCC 2 cut(s) 86, 660
BtgI CCRYGG 2 cut(s) 201, 930
BtsCI GGATG 3 cut(s) 451, 646, 653
BtsIMutI CAGTG 1 cut(s) 100
Cac8I GCNNGC 3 cut(s) 168, 273, 372
CciI TCATGA 2 cut(s) 526, 1243
Cfr10I RCCGGY 1 cut(s) 478
Cfr13I GGNCC 1 cut(s) 757
CseI GACGC 3 cut(s) 816, 1167, 1265
Csp6I GTAC 1 cut(s) 771
CspAI ACCGGT 1 cut(s) 478
CviQI GTAC 1 cut(s) 771
DdeI CTNAG 5 cut(s) 171, 641, 857, 1116, 1288
DpnI GATC 8 cut(s) 62, 196, 424, 525, 804, 870, 927, 974
DpnII GATC 8 cut(s) 60, 194, 422, 523, 802, 868, 925, 972
DrdI GACNNNNNNGTC 1 cut(s) 1193
DriI GACNNNNNGTC 1 cut(s) 433
DseDI GACNNNNNNGTC 1 cut(s) 1193
EaeI YGGCCR 1 cut(s) 84
Eam1104I CTCTTC 2 cut(s) 57, 675
Eam1105I GACNNNNNGTC 1 cut(s) 433
EarI CTCTTC 2 cut(s) 57, 675
EciI GGCGGA 2 cut(s) 187, 502
Eco130I CCWWGG 1 cut(s) 201
Eco24I GRGCYC 1 cut(s) 904
Eco32I GATATC 1 cut(s) 1441
Eco47I GGWCC 1 cut(s) 757
EcoRV GATATC 1 cut(s) 1441
EcoT14I CCWWGG 1 cut(s) 201
EcoT38I GRGCYC 1 cut(s) 904
ErhI CCWWGG 1 cut(s) 201
Esp3I CGTCTC 1 cut(s) 1283
FaqI GGGAC 3 cut(s) 74, 332, 421
FauI CCCGC 1 cut(s) 379
FauNDI CATATG 1 cut(s) 1503
FbaI TGATCA 2 cut(s) 523, 972
Fnu4HI GCNGC 6 cut(s) 75, 339, 499, 990, 1057, 1572
FokI GGATG 3 cut(s) 458, 633, 640
FriOI GRGCYC 1 cut(s) 904
Fsp4HI GCNGC 6 cut(s) 75, 339, 499, 990, 1057, 1572
FspBI CTAG 3 cut(s) 213, 375, 1655
GluI GCNGC 6 cut(s) 75, 339, 499, 990, 1057, 1572
HaeIII GGCC 2 cut(s) 86, 660
HapII CCGG 4 cut(s) 316, 479, 657, 665
HgaI GACGC 3 cut(s) 816, 1167, 1265
HinfI GANTC 4 cut(s) 21, 434, 886, 1332
HpaII CCGG 4 cut(s) 316, 479, 657, 665
HphI GGTGA 2 cut(s) 461, 974
Hpy166II GTNNAC 1 cut(s) 1133
Hpy188I TCNGA 9 cut(s) 18, 194, 427, 814, 972, 1307, 1319, 1466, 1533
Hpy188III TCNNGA 9 cut(s) 25, 64, 527, 800, 1089, 1244, 1376, 1520, 1586
Hpy8I GTNNAC 1 cut(s) 1133
Hpy99I CGWCG 3 cut(s) 82, 194, 810
HpyAV CCTTC 7 cut(s) 178, 313, 482, 671, 771, 882, 1454
HpyCH4III ACNGT 3 cut(s) 82, 1195, 1558
HpyCH4V TGCA 8 cut(s) 260, 271, 599, 721, 1250, 1392, 1432, 1450
HpyF10VI GCNNNNNNNGC 5 cut(s) 83, 272, 338, 512, 986
HpyF3I CTNAG 5 cut(s) 171, 641, 857, 1116, 1288
Ksp22I TGATCA 2 cut(s) 523, 972
Kzo9I GATC 8 cut(s) 60, 194, 422, 523, 802, 868, 925, 972
LguI GCTCTTC 1 cut(s) 675
LmnI GCTCC 1 cut(s) 1685
Lsp1109I GCAGC 6 cut(s) 86, 325, 510, 1001, 1043, 1558
LweI GCATC 2 cut(s) 586, 1237
MaeI CTAG 3 cut(s) 213, 375, 1655
MaeIII GTNAC 2 cut(s) 952, 1291
MalI GATC 8 cut(s) 62, 196, 424, 525, 804, 870, 927, 974
MboI GATC 8 cut(s) 60, 194, 422, 523, 802, 868, 925, 972
MflI RGATCY 3 cut(s) 194, 422, 925
MhlI GDGCHC 2 cut(s) 32, 904
MlyI GAGTC 1 cut(s) 443
MmeI TCCRAC 1 cut(s) 172
MnlI CCTC 9 cut(s) 60, 220, 232, 256, 381, 727, 808, 1313, 1644
MseI TTAA 8 cut(s) 116, 471, 612, 725, 825, 936, 1046, 1145
MslI CAYNNNNRTG 2 cut(s) 881, 1556
MspA1I CMGCKG 2 cut(s) 593, 992
MspI CCGG 4 cut(s) 316, 479, 657, 665
MspR9I CCNGG 1 cut(s) 317
Mva1269I GAATGC 1 cut(s) 1157
MwoI GCNNNNNNNGC 5 cut(s) 83, 272, 338, 512, 986
NciI CCSGG 1 cut(s) 317
NcoI CCATGG 1 cut(s) 201
NdeI CATATG 1 cut(s) 1503
NdeII GATC 8 cut(s) 60, 194, 422, 523, 802, 868, 925, 972
NlaIV GGNNCC 4 cut(s) 196, 207, 759, 927
NmuCI GTSAC 1 cut(s) 952
NspI RCATGY 2 cut(s) 275, 1396
PaeI GCATGC 1 cut(s) 275
PagI TCATGA 2 cut(s) 526, 1243
PciSI GCTCTTC 1 cut(s) 675
PcsI WCGNNNNNNNCGW 2 cut(s) 927, 1345
PctI GAATGC 1 cut(s) 1157
PfeI GAWTC 3 cut(s) 21, 886, 1332
PflMI CCANNNNNTGG 1 cut(s) 446
PfoI TCCNGGA 1 cut(s) 315
PinAI ACCGGT 1 cut(s) 478
PkrI GCNGC 6 cut(s) 76, 340, 500, 991, 1058, 1573
PleI GAGTC 1 cut(s) 442
PpsI GAGTC 1 cut(s) 442
PshBI ATTAAT 1 cut(s) 725
PsiI TTATAA 1 cut(s) 1016
PspN4I GGNNCC 4 cut(s) 196, 207, 759, 927
PspPI GGNCC 1 cut(s) 757
PsuI RGATCY 3 cut(s) 194, 422, 925
PvuII CAGCTG 2 cut(s) 593, 992
RsaI GTAC 1 cut(s) 772
RsaNI GTAC 1 cut(s) 771
RseI CAYNNNNRTG 2 cut(s) 881, 1556
SapI GCTCTTC 1 cut(s) 675
SaqAI TTAA 8 cut(s) 116, 471, 612, 725, 825, 936, 1046, 1145
SatI GCNGC 6 cut(s) 75, 339, 499, 990, 1057, 1572
Sau3AI GATC 8 cut(s) 60, 194, 422, 523, 802, 868, 925, 972
Sau96I GGNCC 1 cut(s) 757
SchI GAGTC 1 cut(s) 443
ScrFI CCNGG 1 cut(s) 317
SduI GDGCHC 2 cut(s) 32, 904
SfaNI GCATC 2 cut(s) 586, 1237
SfcI CTRYAG 2 cut(s) 99, 1624
SinI GGWCC 1 cut(s) 757
SmiMI CAYNNNNRTG 2 cut(s) 881, 1556
SmlI CTYRAG 1 cut(s) 1005
SmoI CTYRAG 1 cut(s) 1005
SphI GCATGC 1 cut(s) 275
SsiI CCGC 7 cut(s) 198, 209, 291, 372, 402, 513, 1155
SspI AATATT 2 cut(s) 753, 1314
SspMI CTAG 3 cut(s) 213, 375, 1655
StyD4I CCNGG 1 cut(s) 315
StyI CCWWGG 1 cut(s) 201
TaaI ACNGT 3 cut(s) 82, 1195, 1558
TaqI TCGA 5 cut(s) 63, 555, 805, 963, 1519
TfiI GAWTC 3 cut(s) 21, 886, 1332
Tru1I TTAA 8 cut(s) 116, 471, 612, 725, 825, 936, 1046, 1145
Tru9I TTAA 8 cut(s) 116, 471, 612, 725, 825, 936, 1046, 1145
TscAI CASTG 1 cut(s) 100
TseFI GTSAC 1 cut(s) 952
TseI GCWGC 6 cut(s) 74, 338, 498, 989, 1056, 1571
Tsp45I GTSAC 1 cut(s) 952
TspDTI ATGAA 9 cut(s) 17, 147, 269, 489, 1139, 1232, 1367, 1483, 1557
TspRI CASTG 1 cut(s) 100
Van91I CCANNNNNTGG 1 cut(s) 446
VpaK11BI GGWCC 1 cut(s) 757
VspI ATTAAT 1 cut(s) 725
XapI RAATTY 3 cut(s) 233, 1371, 1379
XceI RCATGY 2 cut(s) 275, 1396
XspI CTAG 3 cut(s) 213, 375, 1655
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.