Rroxscaffold_2G00081040

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000534
Physical Location & Seq
Forward (+)
4030593 .. 4053489
22897 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00081040.1

Sequence Viewer

Length: 1062 bp
ATGGCGCCTTGTACATGGGGTTATGTGTTGCCTTTTGCAGCTATGGTAATGGTGCAGTTCATAGATATAGGTGCAGCAACTATAAACAAAGCAGCCATGTCGAAAGGAATGAGCAGTTATGTTTTAGTTGTCTACTCTAGTGCGCTTGCTACCATTTTTCTCCTTCCTTGTTTCATCTTACAAAAGAAGATTGCACTTACTTTTTCATTCGTTGGTGGACAATTCCTTCTTGGCCTAATTGGGAGTTCAAATATGTTATTGGCATATACTGGCATCAACTACAGCTCACCAACACTTGCTTCAGCTATGGGAAACGTCATTCCAATCTTTACTTTCATACTGGCAATCATTTTCAGGATGGAAAAGCTGGATTTGGGGAGGTCAAGCTGTCAAGCCAAAGTGTTAGGGACTATTGTATCAGTATCTGGAGCATTTATAGTAATTCTATACAAGGGATCAGTAATCTTTTCACCATCCAACTCTCCTAACCAGAACTTCATGATGACCTTGCAGCAGTCAAAGTGGGTCTTTGGAGGTCTTATGCTTGCAATGGCGAGCGTTTTGAAGGCATCATGGAATATTCTTCAGACATCAATTGTTAAGAACTGTCCATCAATGATTACCGTACTCTTCTTCTACACCTTCTTTGTGACAATTCAATCCACAGTGTTCTCTCTATTTTTGGAAAGCAATCCAAATGCTTGGGTACTTAGGCCTGACATTGAGATGATCTCCATTGTCTTCTCAGCTATCTTCGGGAGTCTGTTCCATATTGGTGTTGAAACCTGGTGCTTGCACCAAAAGGGACCTATCTTTGTAGCCATGTTCAGGCCCTTGGGAGTTGCCATTGCTGCAGTCATGGTAGTTATCTTCCTTGGCGACGCACTTCACCTTGGCAGTGTGATTGGGTCTATCATTTTTGCAATTGGGTTTTATGCTATGATGTGGGCACAAATCAAAGAAAAGAGAACCAATGGTACAGAGAATGAAGTCCAAAGCTTGGCATCATCCACTCAAAAGACCCCTCTATTGCAATGCAGAACCTCAGGAGAAGATGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

353

Amino Acids

38.44

Weight (kDa)

9.12

Isoelectric Point (pI)

38.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 25 - 148 5.1e-09 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000534)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g50400 FvH4_6g50410 FvH4_6g50410 FvH4_6g50410 FvH4_6g50410 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420
malus_domestica MD09G1036400.v1.1 MD09G1036600.v1.1 MD17G1038100.v1.1 MD17G1038200.v1.1
prunus_persica Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G281000_v2.0.a1 Prupe.3G281000_v2.0.a1 Prupe.3G281000_v2.0.a1 Prupe.3G281000_v2.0.a1
pyrus_communis pycom111g02920 pycom111g02930 pycom111g02940 pycom111g02950 pycom16g07600 pycom17g03430 pycom17g03440
rosa_chinensis RchiOBHm_Chr2g0170801 RchiOBHm_Chr2g0170811 RchiOBHm_Chr2g0170821 RchiOBHm_Chr2g0170831 RchiOBHm_Chr2g0170841
rosa_laevigata RLG00000021981 RLG00000021983 RLG00000021984 RLG00000021985
rosa_multiflora Rmu_co8418849.1_g000001 Rmu_co8437261.1_g000001 Rmu_sc0008393.1_g000003
rosa_roxburghii Rroxscaffold_1G00030380 Rroxscaffold_2G00081040 Rroxscaffold_2G00081050 Rroxscaffold_2G00081060
rosa_rugosa Rorug02G0551300 Rorug02G0551400 Rorug02G0551700 Rorug02G0551800
rosa_samantha Rh2AG624300 Rh2BG634700 Rh2BG634800 Rh2BG634900 Rh2BG635000 Rh2CG603500 Rh2CG603600 Rh2CG603700 Rh2CG603800
rosa_wichuraiana Rw0G017070 Rw2G051700 Rw2G051710 Rw2G051720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AccB7I CCANNNNNTGG 1 cut(s) 1000
AccI GTMKAC 1 cut(s) 132
AclWI GGATC 1 cut(s) 463
AcuI CTGAAG 2 cut(s) 285, 569
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 4 cut(s) 13, 627, 708, 979
AfiI CCNNNNNNNGG 2 cut(s) 828, 1000
AgsI TTSAA 4 cut(s) 249, 565, 659, 782
AjnI CCWGG 1 cut(s) 785
AjuI GAANNNNNNNTTGG 2 cut(s) 283, 315
AluBI AGCT 7 cut(s) 41, 285, 305, 367, 387, 749, 999
AluI AGCT 7 cut(s) 41, 285, 305, 367, 387, 749, 999
AlwI GGATC 1 cut(s) 463
AlwNI CAGNNNCTG 1 cut(s) 425
AoxI GGCC 3 cut(s) 232, 713, 830
ApeKI GCWGC 5 cut(s) 38, 74, 92, 511, 851
AspLEI GCGC 2 cut(s) 7, 145
AspS9I GGNCC 2 cut(s) 806, 831
AsuHPI GGTGA 3 cut(s) 279, 462, 881
AvaII GGWCC 1 cut(s) 806
AxyI CCTNAGG 1 cut(s) 1045
BaeGI GKGCMC 1 cut(s) 952
BaeI ACNNNNGTAYC 2 cut(s) 399, 432
BanI GGYRCC 1 cut(s) 4
BbsI GAAGAC 1 cut(s) 733
BbvI GCAGC 5 cut(s) 50, 86, 104, 523, 838
BccI CCATC 3 cut(s) 352, 481, 619
BcgI CGANNNNNNTGC 2 cut(s) 81, 115
BciT130I CCWGG 1 cut(s) 787
BfaI CTAG 1 cut(s) 138
BfmI CTRYAG 2 cut(s) 280, 852
BfoI RGCGCY 1 cut(s) 8
BisI GCNGC 5 cut(s) 39, 75, 93, 512, 852
BlsI GCNGC 5 cut(s) 40, 76, 94, 513, 853
Bme1390I CCNGG 1 cut(s) 787
Bme18I GGWCC 1 cut(s) 806
BmgT120I GGNCC 2 cut(s) 806, 831
BmiI GGNNCC 2 cut(s) 6, 807
BmrFI CCNGG 1 cut(s) 787
BmsI GCATC 3 cut(s) 282, 578, 1013
BpiI GAAGAC 1 cut(s) 733
BplI GAGNNNNNCTC 2 cut(s) 716, 748
BpmI CTGGAG 1 cut(s) 447
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 3 cut(s) 834, 874, 892
BsaXI ACNNNNNCTCC 1 cut(s) 1041
Bsc4I CCNNNNNNNGG 2 cut(s) 828, 1000
Bse1I ACTGG 2 cut(s) 274, 345
Bse21I CCTNAGG 1 cut(s) 1045
Bse3DI GCAATG 3 cut(s) 555, 846, 1040
BseBI CCWGG 1 cut(s) 787
BseDI CCNNGG 3 cut(s) 834, 874, 892
BseGI GGATG 3 cut(s) 363, 473, 1007
BseLI CCNNNNNNNGG 2 cut(s) 828, 1000
BseMI GCAATG 3 cut(s) 555, 846, 1040
BseMII CTCAG 2 cut(s) 759, 1059
BseNI ACTGG 2 cut(s) 274, 345
BseSI GKGCMC 1 cut(s) 952
BseXI GCAGC 5 cut(s) 50, 86, 104, 523, 838
BsgI GTGCAG 2 cut(s) 74, 93
BshFI GGCC 3 cut(s) 234, 715, 832
BshNI GGYRCC 1 cut(s) 4
BslFI GGGAC 2 cut(s) 421, 819
BslI CCNNNNNNNGG 2 cut(s) 828, 1000
BsmFI GGGAC 2 cut(s) 421, 819
BsnI GGCC 3 cut(s) 234, 715, 832
Bsp1286I GDGCHC 1 cut(s) 952
Bsp1407I TGTACA 1 cut(s) 11
Bsp143I GATC 2 cut(s) 455, 729
BspANI GGCC 3 cut(s) 234, 715, 832
BspCNI CTCAG 2 cut(s) 758, 1058
BspHI TCATGA 1 cut(s) 498
BspLI GGNNCC 2 cut(s) 6, 807
BspMAI CTGCAG 1 cut(s) 856
BspPI GGATC 1 cut(s) 463
BspT107I GGYRCC 1 cut(s) 4
BsrDI GCAATG 3 cut(s) 555, 846, 1040
BsrGI TGTACA 1 cut(s) 11
BsrI ACTGG 2 cut(s) 274, 345
BssECI CCNNGG 3 cut(s) 834, 874, 892
BssMI GATC 2 cut(s) 455, 729
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 3 cut(s) 834, 874, 892
Bst2UI CCWGG 1 cut(s) 787
Bst4CI ACNGT 3 cut(s) 608, 625, 667
Bst6I CTCTTC 1 cut(s) 635
BstACI GRCGYC 1 cut(s) 5
BstAUI TGTACA 1 cut(s) 11
BstC8I GCNNGC 4 cut(s) 147, 546, 556, 794
BstDEI CTNAG 3 cut(s) 710, 745, 1045
BstF5I GGATG 3 cut(s) 363, 473, 1007
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 145
BstKTI GATC 2 cut(s) 458, 732
BstMBI GATC 2 cut(s) 455, 729
BstMWI GCNNNNNNNGC 1 cut(s) 851
BstNI CCWGG 1 cut(s) 787
BstSCI CCNGG 1 cut(s) 785
BstSFI CTRYAG 2 cut(s) 280, 852
BstSLI GKGCMC 1 cut(s) 952
BstV1I GCAGC 5 cut(s) 50, 86, 104, 523, 838
BstV2I GAAGAC 1 cut(s) 733
BstXI CCANNNNNNTGG 1 cut(s) 702
Bsu36I CCTNAGG 1 cut(s) 1045
BsuRI GGCC 3 cut(s) 234, 715, 832
BtsCI GGATG 3 cut(s) 363, 473, 1007
BtsI GCAGTG 1 cut(s) 904
BtsIMutI CAGTG 2 cut(s) 672, 904
Cac8I GCNNGC 4 cut(s) 147, 546, 556, 794
CaiI CAGNNNCTG 1 cut(s) 425
CciI TCATGA 1 cut(s) 498
CfoI GCGC 2 cut(s) 7, 145
Cfr13I GGNCC 2 cut(s) 806, 831
CseI GACGC 1 cut(s) 890
CsiI ACCWGGT 1 cut(s) 785
Csp6I GTAC 4 cut(s) 12, 626, 707, 978
CviAII CATG 6 cut(s) 15, 97, 499, 573, 823, 859
CviQI GTAC 4 cut(s) 12, 626, 707, 978
DdeI CTNAG 3 cut(s) 710, 745, 1045
DinI GGCGCC 1 cut(s) 6
DpnI GATC 2 cut(s) 457, 731
DpnII GATC 2 cut(s) 455, 729
Eam1104I CTCTTC 1 cut(s) 635
EarI CTCTTC 1 cut(s) 635
Eco130I CCWWGG 3 cut(s) 834, 874, 892
Eco147I AGGCCT 1 cut(s) 715
Eco47I GGWCC 1 cut(s) 806
Eco57I CTGAAG 2 cut(s) 285, 569
Eco81I CCTNAGG 1 cut(s) 1045
EcoO109I RGGNCCY 2 cut(s) 806, 831
EcoRII CCWGG 1 cut(s) 785
EcoT14I CCWWGG 3 cut(s) 834, 874, 892
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 3 cut(s) 834, 874, 892
FaeI CATG 6 cut(s) 18, 100, 502, 576, 826, 862
FalI AAGNNNNNCTT 2 cut(s) 512, 544
FaqI GGGAC 2 cut(s) 421, 819
FatI CATG 6 cut(s) 14, 96, 498, 572, 822, 858
FblI GTMKAC 1 cut(s) 132
Fnu4HI GCNGC 5 cut(s) 39, 75, 93, 512, 852
FokI GGATG 3 cut(s) 370, 460, 994
Fsp4HI GCNGC 5 cut(s) 39, 75, 93, 512, 852
FspBI CTAG 1 cut(s) 138
GlaI GCGC 2 cut(s) 6, 144
GluI GCNGC 5 cut(s) 39, 75, 93, 512, 852
GsuI CTGGAG 1 cut(s) 447
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 3 cut(s) 234, 715, 832
HgaI GACGC 1 cut(s) 890
HhaI GCGC 2 cut(s) 7, 145
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 6 cut(s) 18, 100, 502, 576, 826, 862
Hin6I GCGC 2 cut(s) 5, 143
HinP1I GCGC 2 cut(s) 5, 143
HindIII AAGCTT 1 cut(s) 997
HinfI GANTC 1 cut(s) 760
HphI GGTGA 3 cut(s) 279, 462, 881
Hpy166II GTNNAC 2 cut(s) 133, 218
Hpy188I TCNGA 1 cut(s) 588
Hpy188III TCNNGA 5 cut(s) 355, 426, 499, 757, 1047
Hpy8I GTNNAC 2 cut(s) 133, 218
Hpy99I CGWCG 1 cut(s) 884
HpyAV CCTTC 4 cut(s) 173, 236, 559, 652
HpyCH4III ACNGT 3 cut(s) 608, 625, 667
HpyCH4IV ACGT 1 cut(s) 315
HpyF10VI GCNNNNNNNGC 1 cut(s) 851
HpyF3I CTNAG 3 cut(s) 710, 745, 1045
HpySE526I ACGT 1 cut(s) 315
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 6 cut(s) 18, 100, 502, 576, 826, 862
HspAI GCGC 2 cut(s) 5, 143
KasI GGCGCC 1 cut(s) 4
Kzo9I GATC 2 cut(s) 455, 729
LmnI GCTCC 1 cut(s) 428
Lsp1109I GCAGC 5 cut(s) 50, 86, 104, 523, 838
LweI GCATC 3 cut(s) 282, 578, 1013
MabI ACCWGGT 1 cut(s) 785
MaeI CTAG 1 cut(s) 138
MaeII ACGT 1 cut(s) 315
MaeIII GTNAC 1 cut(s) 649
MalI GATC 2 cut(s) 457, 731
MboI GATC 2 cut(s) 455, 729
MboII GAAGA 7 cut(s) 199, 575, 622, 625, 733, 745, 862
MfeI CAATTG 2 cut(s) 594, 924
MhlI GDGCHC 1 cut(s) 952
MluCI AATT 6 cut(s) 221, 237, 441, 594, 654, 924
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 1 cut(s) 769
MmeI TCCRAC 1 cut(s) 501
MnlI CCTC 4 cut(s) 372, 527, 1035, 1054
MseI TTAA 1 cut(s) 600
MslI CAYNNNNRTG 2 cut(s) 725, 774
MspR9I CCNGG 1 cut(s) 787
MunI CAATTG 2 cut(s) 594, 924
MvaI CCWGG 1 cut(s) 787
MwoI GCNNNNNNNGC 1 cut(s) 851
NarI GGCGCC 1 cut(s) 5
NdeII GATC 2 cut(s) 455, 729
NlaIII CATG 6 cut(s) 18, 100, 502, 576, 826, 862
NlaIV GGNNCC 2 cut(s) 6, 807
NmuCI GTSAC 1 cut(s) 649
PagI TCATGA 1 cut(s) 498
PceI AGGCCT 1 cut(s) 715
PflMI CCANNNNNTGG 1 cut(s) 1000
PkrI GCNGC 5 cut(s) 40, 76, 94, 513, 853
PleI GAGTC 1 cut(s) 768
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 768
PpuMI RGGWCCY 1 cut(s) 806
Psp5II RGGWCCY 1 cut(s) 806
Psp6I CCWGG 1 cut(s) 785
PspGI CCWGG 1 cut(s) 785
PspN4I GGNNCC 2 cut(s) 6, 807
PspPI GGNCC 2 cut(s) 806, 831
PspPPI RGGWCCY 1 cut(s) 806
PsrI GAACNNNNNNTAC 2 cut(s) 961, 993
PstI CTGCAG 1 cut(s) 856
PstNI CAGNNNCTG 1 cut(s) 425
RsaI GTAC 4 cut(s) 13, 627, 708, 979
RsaNI GTAC 4 cut(s) 12, 626, 707, 978
RseI CAYNNNNRTG 2 cut(s) 725, 774
SaqAI TTAA 1 cut(s) 600
SatI GCNGC 5 cut(s) 39, 75, 93, 512, 852
Sau3AI GATC 2 cut(s) 455, 729
Sau96I GGNCC 2 cut(s) 806, 831
SchI GAGTC 1 cut(s) 769
ScrFI CCNGG 1 cut(s) 787
SduI GDGCHC 1 cut(s) 952
SexAI ACCWGGT 1 cut(s) 785
SfaNI GCATC 3 cut(s) 282, 578, 1013
SfcI CTRYAG 2 cut(s) 280, 852
SfoI GGCGCC 1 cut(s) 6
SinI GGWCC 1 cut(s) 806
SmiMI CAYNNNNRTG 2 cut(s) 725, 774
Sse9I AATT 6 cut(s) 221, 237, 441, 594, 654, 924
SseBI AGGCCT 1 cut(s) 715
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 1 cut(s) 580
SspMI CTAG 1 cut(s) 138
StuI AGGCCT 1 cut(s) 715
StyD4I CCNGG 1 cut(s) 785
StyI CCWWGG 3 cut(s) 834, 874, 892
TaaI ACNGT 3 cut(s) 608, 625, 667
TaiI ACGT 1 cut(s) 318
TaqI TCGA 1 cut(s) 101
TasI AATT 6 cut(s) 221, 237, 441, 594, 654, 924
TatI WGTACW 1 cut(s) 11
Tru1I TTAA 1 cut(s) 600
Tru9I TTAA 1 cut(s) 600
TscAI CASTG 2 cut(s) 672, 904
TseFI GTSAC 1 cut(s) 649
TseI GCWGC 5 cut(s) 38, 74, 92, 511, 851
Tsp45I GTSAC 1 cut(s) 649
TspDTI ATGAA 6 cut(s) 49, 163, 195, 325, 487, 1002
TspRI CASTG 2 cut(s) 672, 904
Van91I CCANNNNNTGG 1 cut(s) 1000
VpaK11BI GGWCC 1 cut(s) 806
XmiI GTMKAC 1 cut(s) 132
XspI CTAG 1 cut(s) 138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.