Rh2BG634800

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000534
Physical Location & Seq
Reverse (-)
85620754 .. 85623426
2673 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG634800.1

Sequence Viewer

Length: 762 bp
ATGGCAAATCTTATACCAATCTACACGTTCATGTTGGCAATCATTTTCAGGATGGAAAAACTAGATTTGAGGAGATCAAGCAGTCAAGCTAAAGTGTTGGGCACTATAGTATCAGTCTCTGGAGCATTTATAATAATCCTATATAAGGGCTTAGTAATCTTAAAGCCTTCATCACCATCCAACTTCCTCACGATCTCACATCAGTCAAACTCAAGGTGGGTCTTTGGAGGTCTTATGCTGGCAATGTCCTGCCTTTTGTCTGCAATTTGGAACATTGTTCAGGCATCCATTGTTAAAAACTGTCCATCAAAGGTGACAGTGGTCTTCTTCTACACGTTCTTTATGACAATCCAATGCACAATATTTTCTCTGATTGTGGAAAGGAATCCGAATACTTGGGTTATAAGACCTGGCATTGAGATGATCTCCATTGTCTGCTCAGCTATAATGGGGAATGTGTTCCATGTTGGTGTTCATACTTGGTGCTTGCACCAAAAGGGACCTGTCTTTGTAGCCATGTTCAGACCTTTGGGAGTTGCCATTGCAGCTGTCATGGTGGTCTTCTTCCTCGGCGATCCGCTTCATCTTGGCAGTGTGGTTGGATCTGTCATCATTGCCATTGGGTTTTATGCTATGATGTGGGCACAAATCAAAGAAAAGGGCAGTAATGTTGTGGAGAATGAAGTCCACAGCTTGGCATCAACAAACCAACACACCACCCCTCTTTTGCAATGCAGAAGCACAGAAGAAGAAAATGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

253

Amino Acids

27.97

Weight (kDa)

9.13

Isoelectric Point (pI)

39.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 75 - 212 4.3e-07 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000534)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g50400 FvH4_6g50410 FvH4_6g50410 FvH4_6g50410 FvH4_6g50410 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420
malus_domestica MD09G1036400.v1.1 MD09G1036600.v1.1 MD17G1038100.v1.1 MD17G1038200.v1.1
prunus_persica Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G281000_v2.0.a1 Prupe.3G281000_v2.0.a1 Prupe.3G281000_v2.0.a1 Prupe.3G281000_v2.0.a1
pyrus_communis pycom111g02920 pycom111g02930 pycom111g02940 pycom111g02950 pycom16g07600 pycom17g03430 pycom17g03440
rosa_chinensis RchiOBHm_Chr2g0170801 RchiOBHm_Chr2g0170811 RchiOBHm_Chr2g0170821 RchiOBHm_Chr2g0170831 RchiOBHm_Chr2g0170841
rosa_laevigata RLG00000021981 RLG00000021983 RLG00000021984 RLG00000021985
rosa_multiflora Rmu_co8418849.1_g000001 Rmu_co8437261.1_g000001 Rmu_sc0008393.1_g000003
rosa_roxburghii Rroxscaffold_1G00030380 Rroxscaffold_2G00081040 Rroxscaffold_2G00081050 Rroxscaffold_2G00081060
rosa_rugosa Rorug02G0551300 Rorug02G0551400 Rorug02G0551700 Rorug02G0551800
rosa_samantha Rh2AG624300 Rh2BG634700 Rh2BG634800 Rh2BG634900 Rh2BG635000 Rh2CG603500 Rh2CG603600 Rh2CG603700 Rh2CG603800
rosa_wichuraiana Rw0G017070 Rw2G051700 Rw2G051710 Rw2G051720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 131, 404
AccB7I CCANNNNNTGG 1 cut(s) 694
AciI CCGC 1 cut(s) 578
AclWI GGATC 2 cut(s) 569, 610
AfiI CCNNNNNNNGG 2 cut(s) 145, 694
AflIII ACRYGT 2 cut(s) 24, 333
AjnI CCWGG 1 cut(s) 409
AluBI AGCT 4 cut(s) 89, 443, 548, 693
AluI AGCT 4 cut(s) 89, 443, 548, 693
Alw26I GTCTC 1 cut(s) 121
AlwI GGATC 2 cut(s) 569, 610
AlwNI CAGNNNCTG 1 cut(s) 119
ApeKI GCWGC 1 cut(s) 545
Asp700I GAANNNNTTC 1 cut(s) 458
AspS9I GGNCC 1 cut(s) 500
AsuHPI GGTGA 2 cut(s) 165, 325
AvaII GGWCC 1 cut(s) 500
BaeGI GKGCMC 2 cut(s) 104, 646
BaeI ACNNNNGTAYC 2 cut(s) 93, 126
BbsI GAAGAC 2 cut(s) 316, 553
BbvI GCAGC 1 cut(s) 557
BccI CCATC 3 cut(s) 46, 184, 313
BciT130I CCWGG 1 cut(s) 411
BcoDI GTCTC 1 cut(s) 121
BfaI CTAG 1 cut(s) 62
BfmI CTRYAG 1 cut(s) 105
BisI GCNGC 1 cut(s) 546
BlpI GCTNAGC 1 cut(s) 439
BlsI GCNGC 1 cut(s) 547
Bme1390I CCNGG 1 cut(s) 411
Bme18I GGWCC 1 cut(s) 500
BmgT120I GGNCC 1 cut(s) 500
BmiI GGNNCC 1 cut(s) 501
BmrFI CCNGG 1 cut(s) 411
BmsI GCATC 2 cut(s) 293, 707
BoxI GACNNNNGTC 1 cut(s) 320
BpiI GAAGAC 2 cut(s) 316, 553
BplI GAGNNNNNCTC 2 cut(s) 410, 442
BpmI CTGGAG 1 cut(s) 141
Bpu1102I GCTNAGC 1 cut(s) 439
BpuEI CTTGAG 1 cut(s) 196
BsaJI CCNNGG 1 cut(s) 568
Bsc4I CCNNNNNNNGG 2 cut(s) 145, 694
Bse3DI GCAATG 4 cut(s) 249, 540, 612, 737
BseBI CCWGG 1 cut(s) 411
BseDI CCNNGG 1 cut(s) 568
BseGI GGATG 3 cut(s) 57, 176, 284
BseLI CCNNNNNNNGG 2 cut(s) 145, 694
BseMI GCAATG 4 cut(s) 249, 540, 612, 737
BseMII CTCAG 1 cut(s) 453
BseRI GAGGAG 1 cut(s) 85
BseSI GKGCMC 2 cut(s) 104, 646
BseXI GCAGC 1 cut(s) 557
BslFI GGGAC 1 cut(s) 513
BslI CCNNNNNNNGG 2 cut(s) 145, 694
BsmAI GTCTC 1 cut(s) 121
BsmFI GGGAC 1 cut(s) 513
Bsp1286I GDGCHC 2 cut(s) 104, 646
Bsp143I GATC 5 cut(s) 74, 192, 423, 574, 602
Bsp1720I GCTNAGC 1 cut(s) 439
BspACI CCGC 1 cut(s) 578
BspCNI CTCAG 1 cut(s) 452
BspLI GGNNCC 1 cut(s) 501
BspPI GGATC 2 cut(s) 569, 610
BsrDI GCAATG 4 cut(s) 249, 540, 612, 737
BssECI CCNNGG 1 cut(s) 568
BssMI GATC 5 cut(s) 74, 192, 423, 574, 602
Bst2UI CCWGG 1 cut(s) 411
Bst4CI ACNGT 2 cut(s) 302, 319
BstC8I GCNNGC 2 cut(s) 240, 488
BstDEI CTNAG 2 cut(s) 151, 439
BstENI CCTNNNNNAGG 1 cut(s) 143
BstF5I GGATG 3 cut(s) 57, 176, 284
BstKTI GATC 5 cut(s) 77, 195, 426, 577, 605
BstMAI GTCTC 1 cut(s) 121
BstMBI GATC 5 cut(s) 74, 192, 423, 574, 602
BstMWI GCNNNNNNNGC 1 cut(s) 545
BstNI CCWGG 1 cut(s) 411
BstPAI GACNNNNGTC 1 cut(s) 320
BstSCI CCNGG 1 cut(s) 409
BstSFI CTRYAG 1 cut(s) 105
BstSLI GKGCMC 2 cut(s) 104, 646
BstV1I GCAGC 1 cut(s) 557
BstV2I GAAGAC 2 cut(s) 316, 553
BstX2I RGATCY 1 cut(s) 602
BstYI RGATCY 1 cut(s) 602
BtsCI GGATG 3 cut(s) 57, 176, 284
BtsI GCAGTG 1 cut(s) 598
BtsIMutI CAGTG 2 cut(s) 324, 598
Cac8I GCNNGC 2 cut(s) 240, 488
CaiI CAGNNNCTG 1 cut(s) 119
Cfr13I GGNCC 1 cut(s) 500
CviAII CATG 4 cut(s) 31, 464, 517, 553
CviJI RGCY 7 cut(s) 89, 150, 166, 443, 515, 548, 693
CviKI_1 RGCY 7 cut(s) 89, 150, 166, 443, 515, 548, 693
DdeI CTNAG 2 cut(s) 151, 439
DpnI GATC 5 cut(s) 76, 194, 425, 576, 604
DpnII GATC 5 cut(s) 74, 192, 423, 574, 602
Eco47I GGWCC 1 cut(s) 500
EcoNI CCTNNNNNAGG 1 cut(s) 143
EcoO109I RGGNCCY 1 cut(s) 500
EcoRII CCWGG 1 cut(s) 409
FaeI CATG 4 cut(s) 34, 467, 520, 556
FaqI GGGAC 1 cut(s) 513
FatI CATG 4 cut(s) 30, 463, 516, 552
Fnu4HI GCNGC 1 cut(s) 546
FokI GGATG 3 cut(s) 64, 163, 271
Fsp4HI GCNGC 1 cut(s) 546
FspBI CTAG 1 cut(s) 62
GluI GCNGC 1 cut(s) 546
GsuI CTGGAG 1 cut(s) 141
Hin1II CATG 4 cut(s) 34, 467, 520, 556
HinfI GANTC 1 cut(s) 385
HphI GGTGA 2 cut(s) 165, 325
Hpy166II GTNNAC 1 cut(s) 688
Hpy188I TCNGA 3 cut(s) 372, 390, 524
Hpy188III TCNNGA 3 cut(s) 49, 120, 190
Hpy8I GTNNAC 1 cut(s) 688
HpyAV CCTTC 1 cut(s) 177
HpyCH4III ACNGT 2 cut(s) 302, 319
HpyCH4IV ACGT 2 cut(s) 26, 335
HpyCH4V TGCA 6 cut(s) 263, 357, 490, 545, 730, 735
HpyF10VI GCNNNNNNNGC 1 cut(s) 545
HpyF3I CTNAG 2 cut(s) 151, 439
HpySE526I ACGT 2 cut(s) 26, 335
Hsp92II CATG 4 cut(s) 34, 467, 520, 556
Kzo9I GATC 5 cut(s) 74, 192, 423, 574, 602
LmnI GCTCC 1 cut(s) 122
LpnPI CCDG 8 cut(s) 34, 105, 224, 262, 266, 396, 423, 516
Lsp1109I GCAGC 1 cut(s) 557
LweI GCATC 2 cut(s) 293, 707
MaeI CTAG 1 cut(s) 62
MaeII ACGT 2 cut(s) 26, 335
MaeIII GTNAC 1 cut(s) 313
MalI GATC 5 cut(s) 76, 194, 425, 576, 604
MboI GATC 5 cut(s) 74, 192, 423, 574, 602
MboII GAAGA 6 cut(s) 316, 319, 553, 556, 758, 761
MflI RGATCY 1 cut(s) 602
MhlI GDGCHC 2 cut(s) 104, 646
MluCI AATT 1 cut(s) 264
MmeI TCCRAC 2 cut(s) 204, 580
MnlI CCTC 5 cut(s) 63, 197, 221, 578, 732
MroXI GAANNNNTTC 1 cut(s) 458
MseI TTAA 3 cut(s) 161, 294, 760
MslI CAYNNNNRTG 3 cut(s) 29, 419, 468
MspA1I CMGCKG 1 cut(s) 548
MspR9I CCNGG 1 cut(s) 411
MvaI CCWGG 1 cut(s) 411
MwoI GCNNNNNNNGC 1 cut(s) 545
NdeII GATC 5 cut(s) 74, 192, 423, 574, 602
NlaIII CATG 4 cut(s) 34, 467, 520, 556
NlaIV GGNNCC 1 cut(s) 501
NmeAIII GCCGAG 1 cut(s) 549
NmuCI GTSAC 1 cut(s) 313
PdmI GAANNNNTTC 1 cut(s) 458
PfeI GAWTC 1 cut(s) 385
PflMI CCANNNNNTGG 1 cut(s) 694
PkrI GCNGC 1 cut(s) 547
PpuMI RGGWCCY 1 cut(s) 500
PshAI GACNNNNGTC 1 cut(s) 320
PsiI TTATAA 2 cut(s) 131, 404
Psp5II RGGWCCY 1 cut(s) 500
Psp6I CCWGG 1 cut(s) 409
PspGI CCWGG 1 cut(s) 409
PspN4I GGNNCC 1 cut(s) 501
PspPI GGNCC 1 cut(s) 500
PspPPI RGGWCCY 1 cut(s) 500
PstNI CAGNNNCTG 1 cut(s) 119
PsuI RGATCY 1 cut(s) 602
PvuII CAGCTG 1 cut(s) 548
RseI CAYNNNNRTG 3 cut(s) 29, 419, 468
SaqAI TTAA 3 cut(s) 161, 294, 760
SatI GCNGC 1 cut(s) 546
Sau3AI GATC 5 cut(s) 74, 192, 423, 574, 602
Sau96I GGNCC 1 cut(s) 500
ScrFI CCNGG 1 cut(s) 411
SduI GDGCHC 2 cut(s) 104, 646
SfaNI GCATC 2 cut(s) 293, 707
SfcI CTRYAG 1 cut(s) 105
SinI GGWCC 1 cut(s) 500
SmiMI CAYNNNNRTG 3 cut(s) 29, 419, 468
SmlI CTYRAG 1 cut(s) 211
SmoI CTYRAG 1 cut(s) 211
Sse9I AATT 1 cut(s) 264
SsiI CCGC 1 cut(s) 578
SspI AATATT 1 cut(s) 363
SspMI CTAG 1 cut(s) 62
StyD4I CCNGG 1 cut(s) 409
TaaI ACNGT 2 cut(s) 302, 319
TaiI ACGT 2 cut(s) 29, 338
TasI AATT 1 cut(s) 264
TfiI GAWTC 1 cut(s) 385
Tru1I TTAA 3 cut(s) 161, 294, 760
Tru9I TTAA 3 cut(s) 161, 294, 760
TscAI CASTG 2 cut(s) 324, 598
TseFI GTSAC 1 cut(s) 313
TseI GCWGC 1 cut(s) 545
Tsp45I GTSAC 1 cut(s) 313
TspDTI ATGAA 5 cut(s) 19, 159, 464, 572, 696
TspRI CASTG 2 cut(s) 324, 598
Van91I CCANNNNNTGG 1 cut(s) 694
VpaK11BI GGWCC 1 cut(s) 500
XagI CCTNNNNNAGG 1 cut(s) 143
XmnI GAANNNNTTC 1 cut(s) 458
XspI CTAG 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.