MD17G1038100.v1.1

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000534
Physical Location & Seq
Forward (+)
2776200 .. 2778287
2088 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1038100.v1.1.491

Sequence Viewer

Length: 1086 bp
ATGGTGTGGCAGCGTCGCATATGGGGCGCCGTGCCCTTCACTGCCATGGTAATCGTTGAGTGTGCAGAAGTTGGCGTGTCGACGATAAGCAAAGTAGCCATGTCGAAAGGGATGAGCCACTTTGTCTTCATTGTCTACTACAATGCTCTTGGTACCCTCTTGCTCCTCCCCTTCTTCATCTTCCGAAGAAAGAAGCGAGCTCCTTTGACTTTCAGGCTCATCTGCCAATTCTTTCTCCTCGGCCTAATAGGGAGCTCAGGGAAAATACTGTTTTTTGCCGGTGTCAAAAACAGTTCGGCTCTTCTTGCGTCAGCAATGGCGAATCTCACCCCAATCTTCACTTTCTTGCTAGCCCTAATTTTCAGGATGGAAAAGCTAGACTTGAGAAAAGCAACGAGTTATGCCAAATCCTTGGGCGCCATTATATCAGTGGGAGGGGCATTGATAGTGACCCTTTACGACGGCCCTGCCCTATTGAACTCTTCCTCGTCTTCCGGCTCGAGTCACTCGTCTCATGGATCTTTCGTCTCACGGTCATCAACCTGGATCTTTGGAGGTCTGCTACTCGCAATCCAATGCCTCGTAGCTTCGGGATGGAACATCGCTCAGGCAGCCACTGTGAAGGATTACCCGGAAGAAATGACCATAGTCTTCTTCTACACCATGTTTCTTACAATTCAATGCACAGTTATCTCTCTAATTGTGGAGAGGAATAATATGAGTGCATGGAAACTGGAGCTTCCCATTGAGATAATCGCCGTTGTGTATGCGGCAATCTGTGTGAGTGTGTTTCGCATCAGTGTTCATGTCTGGTGCTTGCACAAGAAGGGGCCTGTTTATGTAGCCATGTTTAAGCCCTTAGGAATCGCCATTGCGGCACTCCTGGTGGTTGTTTTCCTTGGCGAAACCTTTTATCTCGGCAGTTTGATTGGTTCTATTGTGATCTCGGTGGGATTTTATACCGTGATATACGCAAAAATGAAGGAGAAAGCAATGGCGGATAATGAGGCAGTTCAACCATTAAAACCGTCTGATCAAATCCAAATGGCTCCTCTGTTGCCAAGCAGTATCAGTAACGAAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

362

Amino Acids

39.43

Weight (kDa)

9.46

Isoelectric Point (pI)

41.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 27 - 151 3.9e-11 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000534)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g50400 FvH4_6g50410 FvH4_6g50410 FvH4_6g50410 FvH4_6g50410 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420
malus_domestica MD09G1036400.v1.1 MD09G1036600.v1.1 MD17G1038100.v1.1 MD17G1038200.v1.1
prunus_persica Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G281000_v2.0.a1 Prupe.3G281000_v2.0.a1 Prupe.3G281000_v2.0.a1 Prupe.3G281000_v2.0.a1
pyrus_communis pycom111g02920 pycom111g02930 pycom111g02940 pycom111g02950 pycom16g07600 pycom17g03430 pycom17g03440
rosa_chinensis RchiOBHm_Chr2g0170801 RchiOBHm_Chr2g0170811 RchiOBHm_Chr2g0170821 RchiOBHm_Chr2g0170831 RchiOBHm_Chr2g0170841
rosa_laevigata RLG00000021981 RLG00000021983 RLG00000021984 RLG00000021985
rosa_multiflora Rmu_co8418849.1_g000001 Rmu_co8437261.1_g000001 Rmu_sc0008393.1_g000003
rosa_roxburghii Rroxscaffold_1G00030380 Rroxscaffold_2G00081040 Rroxscaffold_2G00081050 Rroxscaffold_2G00081060
rosa_rugosa Rorug02G0551300 Rorug02G0551400 Rorug02G0551700 Rorug02G0551800
rosa_samantha Rh2AG624300 Rh2BG634700 Rh2BG634800 Rh2BG634900 Rh2BG635000 Rh2CG603500 Rh2CG603600 Rh2CG603700 Rh2CG603800
rosa_wichuraiana Rw0G017070 Rw2G051700 Rw2G051710 Rw2G051720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 152
AccB1I GGYRCC 3 cut(s) 26, 152, 416
AccI GTMKAC 2 cut(s) 80, 135
AciI CCGC 3 cut(s) 770, 875, 998
AclWI GGATC 2 cut(s) 526, 554
AcyI GRCGYC 2 cut(s) 27, 417
AfaI GTAC 1 cut(s) 154
AgsI TTSAA 3 cut(s) 478, 680, 1016
AjnI CCWGG 2 cut(s) 542, 882
AluBI AGCT 5 cut(s) 200, 255, 376, 587, 739
AluI AGCT 5 cut(s) 200, 255, 376, 587, 739
Alw21I GWGCWC 2 cut(s) 202, 257
Alw26I GTCTC 2 cut(s) 516, 532
AlwI GGATC 2 cut(s) 526, 554
AlwNI CAGNNNCTG 1 cut(s) 617
Ama87I CYCGRG 1 cut(s) 499
AoxI GGCC 3 cut(s) 241, 463, 830
ApeKI GCWGC 2 cut(s) 10, 611
Asp718I GGTACC 1 cut(s) 152
AspLEI GCGC 2 cut(s) 29, 419
AspS9I GGNCC 2 cut(s) 464, 830
AsuC2I CCSGG 1 cut(s) 632
AsuHPI GGTGA 1 cut(s) 319
AsuNHI GCTAGC 1 cut(s) 349
AvaI CYCGRG 1 cut(s) 499
AxyI CCTNAGG 1 cut(s) 859
BaeGI GKGCMC 1 cut(s) 36
BanI GGYRCC 3 cut(s) 26, 152, 416
BanII GRGCYC 2 cut(s) 202, 257
BbsI GAAGAC 3 cut(s) 118, 483, 643
Bbv12I GWGCWC 2 cut(s) 202, 257
BbvI GCAGC 2 cut(s) 22, 623
BccI CCATC 2 cut(s) 361, 588
BceAI ACGGC 3 cut(s) 14, 478, 743
BcgI CGANNNNNNTGC 2 cut(s) 449, 483
BciT130I CCWGG 2 cut(s) 544, 884
BclI TGATCA 1 cut(s) 1033
BcnI CCSGG 1 cut(s) 632
BcoDI GTCTC 2 cut(s) 516, 532
BfaI CTAG 2 cut(s) 350, 377
BfoI RGCGCY 2 cut(s) 30, 420
BglI GCCNNNNNGGC 1 cut(s) 875
BisI GCNGC 4 cut(s) 11, 612, 771, 876
BlsI GCNGC 4 cut(s) 12, 613, 772, 877
Bme1390I CCNGG 3 cut(s) 544, 632, 884
BmeT110I CYCGRG 1 cut(s) 499
BmgT120I GGNCC 2 cut(s) 464, 830
BmiI GGNNCC 5 cut(s) 28, 154, 418, 831, 1050
BmrFI CCNGG 3 cut(s) 544, 632, 884
BmsI GCATC 1 cut(s) 804
BmtI GCTAGC 1 cut(s) 353
BoxI GACNNNNGTC 1 cut(s) 647
BpiI GAAGAC 3 cut(s) 118, 483, 643
BpmI CTGGAG 1 cut(s) 755
Bpu10I CCTNAGC 2 cut(s) 256, 606
BpuEI CTTGAG 1 cut(s) 403
BpuMI CCSGG 1 cut(s) 632
BsaHI GRCGYC 2 cut(s) 27, 417
BsaJI CCNNGG 4 cut(s) 45, 238, 411, 898
Bse118I RCCGGY 1 cut(s) 278
Bse1I ACTGG 1 cut(s) 738
Bse21I CCTNAGG 1 cut(s) 859
Bse3DI GCAATG 3 cut(s) 321, 870, 999
BseBI CCWGG 2 cut(s) 544, 884
BseDI CCNNGG 4 cut(s) 45, 238, 411, 898
BseGI GGATG 3 cut(s) 117, 372, 599
BseMI GCAATG 3 cut(s) 321, 870, 999
BseMII CTCAG 2 cut(s) 270, 620
BseNI ACTGG 1 cut(s) 738
BseRI GAGGAG 3 cut(s) 155, 227, 1041
BseSI GKGCMC 1 cut(s) 36
BseXI GCAGC 2 cut(s) 22, 623
BsgI GTGCAG 1 cut(s) 84
BshFI GGCC 3 cut(s) 243, 465, 832
BshNI GGYRCC 3 cut(s) 26, 152, 416
BsiHKAI GWGCWC 2 cut(s) 202, 257
BsiHKCI CYCGRG 1 cut(s) 499
BsiSI CCGG 3 cut(s) 279, 495, 632
BsmAI GTCTC 2 cut(s) 516, 532
BsmBI CGTCTC 2 cut(s) 516, 532
BsnI GGCC 3 cut(s) 243, 465, 832
BsoBI CYCGRG 1 cut(s) 499
Bsp1286I GDGCHC 3 cut(s) 36, 202, 257
Bsp143I GATC 4 cut(s) 518, 546, 942, 1033
Bsp19I CCATGG 1 cut(s) 45
BspACI CCGC 3 cut(s) 770, 875, 998
BspANI GGCC 3 cut(s) 243, 465, 832
BspCNI CTCAG 2 cut(s) 269, 619
BspLI GGNNCC 5 cut(s) 28, 154, 418, 831, 1050
BspOI GCTAGC 1 cut(s) 353
BspPI GGATC 2 cut(s) 526, 554
BspQI GCTCTTC 1 cut(s) 306
BspT107I GGYRCC 3 cut(s) 26, 152, 416
BsrDI GCAATG 3 cut(s) 321, 870, 999
BsrFI RCCGGY 1 cut(s) 278
BsrI ACTGG 1 cut(s) 738
BssAI RCCGGY 1 cut(s) 278
BssECI CCNNGG 4 cut(s) 45, 238, 411, 898
BssMI GATC 4 cut(s) 518, 546, 942, 1033
BssNI GRCGYC 2 cut(s) 27, 417
BssT1I CCWWGG 3 cut(s) 45, 411, 898
Bst2UI CCWGG 2 cut(s) 544, 884
Bst4CI ACNGT 7 cut(s) 270, 293, 534, 619, 688, 964, 1029
Bst6I CTCTTC 2 cut(s) 306, 487
BstACI GRCGYC 2 cut(s) 27, 417
BstC8I GCNNGC 3 cut(s) 198, 351, 818
BstDEI CTNAG 3 cut(s) 256, 606, 859
BstDSI CCRYGG 1 cut(s) 45
BstF5I GGATG 3 cut(s) 117, 372, 599
BstH2I RGCGCY 2 cut(s) 30, 420
BstHHI GCGC 2 cut(s) 29, 419
BstKTI GATC 4 cut(s) 521, 549, 945, 1036
BstMAI GTCTC 2 cut(s) 516, 532
BstMBI GATC 4 cut(s) 518, 546, 942, 1033
BstMWI GCNNNNNNNGC 4 cut(s) 24, 305, 611, 875
BstNI CCWGG 2 cut(s) 544, 884
BstPAI GACNNNNGTC 1 cut(s) 647
BstSCI CCNGG 3 cut(s) 542, 630, 882
BstSLI GKGCMC 1 cut(s) 36
BstV1I GCAGC 2 cut(s) 22, 623
BstV2I GAAGAC 3 cut(s) 118, 483, 643
BstX2I RGATCY 2 cut(s) 518, 546
BstXI CCANNNNNNTGG 1 cut(s) 412
BstYI RGATCY 2 cut(s) 518, 546
Bsu36I CCTNAGG 1 cut(s) 859
BsuRI GGCC 3 cut(s) 243, 465, 832
BtgI CCRYGG 1 cut(s) 45
BtgZI GCGATG 1 cut(s) 586
BtsCI GGATG 3 cut(s) 117, 372, 599
BtsI GCAGTG 1 cut(s) 39
BtsIMutI CAGTG 4 cut(s) 39, 435, 615, 805
Cac8I GCNNGC 3 cut(s) 198, 351, 818
CaiI CAGNNNCTG 1 cut(s) 617
CfoI GCGC 2 cut(s) 29, 419
Cfr10I RCCGGY 1 cut(s) 278
Cfr13I GGNCC 2 cut(s) 464, 830
CseI GACGC 2 cut(s) 2, 297
Csp6I GTAC 1 cut(s) 153
CviAII CATG 7 cut(s) 46, 100, 515, 664, 726, 806, 847
CviQI GTAC 1 cut(s) 153
DdeI CTNAG 3 cut(s) 256, 606, 859
DinI GGCGCC 2 cut(s) 28, 418
DpnI GATC 4 cut(s) 520, 548, 944, 1035
DpnII GATC 4 cut(s) 518, 546, 942, 1033
Eam1104I CTCTTC 2 cut(s) 306, 487
EarI CTCTTC 2 cut(s) 306, 487
EciI GGCGGA 1 cut(s) 1013
Ecl136II GAGCTC 2 cut(s) 200, 255
Eco130I CCWWGG 3 cut(s) 45, 411, 898
Eco24I GRGCYC 2 cut(s) 202, 257
Eco53kI GAGCTC 2 cut(s) 200, 255
Eco81I CCTNAGG 1 cut(s) 859
Eco88I CYCGRG 1 cut(s) 499
EcoICRI GAGCTC 2 cut(s) 200, 255
EcoO109I RGGNCCY 1 cut(s) 830
EcoRII CCWGG 2 cut(s) 542, 882
EcoT14I CCWWGG 3 cut(s) 45, 411, 898
EcoT38I GRGCYC 2 cut(s) 202, 257
EgeI GGCGCC 2 cut(s) 28, 418
EheI GGCGCC 2 cut(s) 28, 418
ErhI CCWWGG 3 cut(s) 45, 411, 898
Esp3I CGTCTC 2 cut(s) 516, 532
FaeI CATG 7 cut(s) 49, 103, 518, 667, 729, 809, 850
FalI AAGNNNNNCTT 2 cut(s) 365, 397
FatI CATG 7 cut(s) 45, 99, 514, 663, 725, 805, 846
FauNDI CATATG 1 cut(s) 20
FbaI TGATCA 1 cut(s) 1033
FblI GTMKAC 2 cut(s) 80, 135
Fnu4HI GCNGC 4 cut(s) 11, 612, 771, 876
FokI GGATG 3 cut(s) 124, 379, 606
FriOI GRGCYC 2 cut(s) 202, 257
Fsp4HI GCNGC 4 cut(s) 11, 612, 771, 876
FspBI CTAG 2 cut(s) 350, 377
GlaI GCGC 2 cut(s) 28, 418
GluI GCNGC 4 cut(s) 11, 612, 771, 876
GsuI CTGGAG 1 cut(s) 755
HaeII RGCGCY 2 cut(s) 30, 420
HaeIII GGCC 3 cut(s) 243, 465, 832
HapII CCGG 3 cut(s) 279, 495, 632
HgaI GACGC 2 cut(s) 2, 297
HhaI GCGC 2 cut(s) 29, 419
Hin1I GRCGYC 2 cut(s) 27, 417
Hin1II CATG 7 cut(s) 49, 103, 518, 667, 729, 809, 850
Hin6I GCGC 2 cut(s) 27, 417
HinP1I GCGC 2 cut(s) 27, 417
HincII GTYRAC 1 cut(s) 81
HindII GTYRAC 1 cut(s) 81
HinfI GANTC 3 cut(s) 322, 502, 864
HpaII CCGG 3 cut(s) 279, 495, 632
HphI GGTGA 1 cut(s) 319
Hpy166II GTNNAC 2 cut(s) 81, 136
Hpy188I TCNGA 2 cut(s) 185, 1033
Hpy188III TCNNGA 2 cut(s) 364, 591
Hpy8I GTNNAC 2 cut(s) 81, 136
Hpy99I CGWCG 3 cut(s) 18, 85, 464
HpyAV CCTTC 5 cut(s) 46, 181, 616, 820, 976
HpyCH4III ACNGT 7 cut(s) 270, 293, 534, 619, 688, 964, 1029
HpyCH4V TGCA 4 cut(s) 65, 684, 725, 820
HpyF10VI GCNNNNNNNGC 4 cut(s) 24, 305, 611, 875
HpyF3I CTNAG 3 cut(s) 256, 606, 859
Hsp92I GRCGYC 2 cut(s) 27, 417
Hsp92II CATG 7 cut(s) 49, 103, 518, 667, 729, 809, 850
HspAI GCGC 2 cut(s) 27, 417
KasI GGCGCC 2 cut(s) 26, 416
KpnI GGTACC 1 cut(s) 156
Ksp22I TGATCA 1 cut(s) 1033
Kzo9I GATC 4 cut(s) 518, 546, 942, 1033
LguI GCTCTTC 1 cut(s) 306
LmnI GCTCC 5 cut(s) 168, 205, 252, 736, 1054
Lsp1109I GCAGC 2 cut(s) 22, 623
LweI GCATC 1 cut(s) 804
MaeI CTAG 2 cut(s) 350, 377
MaeIII GTNAC 3 cut(s) 448, 503, 1073
MalI GATC 4 cut(s) 520, 548, 944, 1035
MboI GATC 4 cut(s) 518, 546, 942, 1033
MflI RGATCY 2 cut(s) 518, 546
MhlI GDGCHC 3 cut(s) 36, 202, 257
MluCI AATT 4 cut(s) 227, 357, 675, 699
Mly113I GGCGCC 2 cut(s) 27, 417
MlyI GAGTC 1 cut(s) 511
MseI TTAA 2 cut(s) 852, 1022
MslI CAYNNNNRTG 1 cut(s) 44
MspI CCGG 3 cut(s) 279, 495, 632
MspR9I CCNGG 3 cut(s) 544, 632, 884
MvaI CCWGG 2 cut(s) 544, 884
MwoI GCNNNNNNNGC 4 cut(s) 24, 305, 611, 875
NarI GGCGCC 2 cut(s) 27, 417
NciI CCSGG 1 cut(s) 632
NcoI CCATGG 1 cut(s) 45
NdeI CATATG 1 cut(s) 20
NdeII GATC 4 cut(s) 518, 546, 942, 1033
NheI GCTAGC 1 cut(s) 349
NlaIII CATG 7 cut(s) 49, 103, 518, 667, 729, 809, 850
NlaIV GGNNCC 5 cut(s) 28, 154, 418, 831, 1050
NmeAIII GCCGAG 2 cut(s) 219, 897
NmuCI GTSAC 2 cut(s) 448, 503
PaeR7I CTCGAG 1 cut(s) 499
PciSI GCTCTTC 1 cut(s) 306
PcsI WCGNNNNNNNCGW 1 cut(s) 506
PfeI GAWTC 2 cut(s) 322, 864
PkrI GCNGC 4 cut(s) 12, 613, 772, 877
PleI GAGTC 1 cut(s) 510
PluTI GGCGCC 2 cut(s) 30, 420
PpsI GAGTC 1 cut(s) 510
PshAI GACNNNNGTC 1 cut(s) 647
Psp124BI GAGCTC 2 cut(s) 202, 257
Psp6I CCWGG 2 cut(s) 542, 882
PspGI CCWGG 2 cut(s) 542, 882
PspN4I GGNNCC 5 cut(s) 28, 154, 418, 831, 1050
PspPI GGNCC 2 cut(s) 464, 830
PspXI VCTCGAGB 1 cut(s) 499
PstNI CAGNNNCTG 1 cut(s) 617
PsuI RGATCY 2 cut(s) 518, 546
RsaI GTAC 1 cut(s) 154
RsaNI GTAC 1 cut(s) 153
RseI CAYNNNNRTG 1 cut(s) 44
SacI GAGCTC 2 cut(s) 202, 257
SalI GTCGAC 1 cut(s) 79
SapI GCTCTTC 1 cut(s) 306
SaqAI TTAA 2 cut(s) 852, 1022
SatI GCNGC 4 cut(s) 11, 612, 771, 876
Sau3AI GATC 4 cut(s) 518, 546, 942, 1033
Sau96I GGNCC 2 cut(s) 464, 830
SchI GAGTC 1 cut(s) 511
ScrFI CCNGG 3 cut(s) 544, 632, 884
SduI GDGCHC 3 cut(s) 36, 202, 257
SetI ASST 8 cut(s) 202, 257, 378, 545, 559, 589, 741, 911
SfaNI GCATC 1 cut(s) 804
SfoI GGCGCC 2 cut(s) 28, 418
Sfr274I CTCGAG 1 cut(s) 499
SlaI CTCGAG 1 cut(s) 499
SmiMI CAYNNNNRTG 1 cut(s) 44
SmlI CTYRAG 2 cut(s) 382, 499
SmoI CTYRAG 2 cut(s) 382, 499
Sse9I AATT 4 cut(s) 227, 357, 675, 699
SsiI CCGC 3 cut(s) 770, 875, 998
SspDI GGCGCC 2 cut(s) 26, 416
SspMI CTAG 2 cut(s) 350, 377
SstI GAGCTC 2 cut(s) 202, 257
StyD4I CCNGG 3 cut(s) 542, 630, 882
StyI CCWWGG 3 cut(s) 45, 411, 898
TaaI ACNGT 7 cut(s) 270, 293, 534, 619, 688, 964, 1029
TaqI TCGA 3 cut(s) 80, 104, 500
TasI AATT 4 cut(s) 227, 357, 675, 699
TauI GCSGC 2 cut(s) 773, 878
TfiI GAWTC 2 cut(s) 322, 864
Tru1I TTAA 2 cut(s) 852, 1022
Tru9I TTAA 2 cut(s) 852, 1022
TscAI CASTG 4 cut(s) 46, 435, 622, 805
TseFI GTSAC 2 cut(s) 448, 503
TseI GCWGC 2 cut(s) 10, 611
Tsp45I GTSAC 2 cut(s) 448, 503
TspDTI ATGAA 4 cut(s) 118, 166, 794, 995
TspRI CASTG 4 cut(s) 46, 435, 622, 805
XcmI CCANNNNNNNNNTGG 1 cut(s) 427
XhoI CTCGAG 1 cut(s) 499
XmiI GTMKAC 2 cut(s) 80, 135
XspI CTAG 2 cut(s) 350, 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.