pycom17g03440

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0000534
Physical Location & Seq
Forward (+)
2273294 .. 2275787
2494 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g03440.2

Sequence Viewer

Length: 1113 bp
ATGGTTTGGCAAAGTCGCATATGGGGCGCCGTGCCCTTCACTGCCATGGTAATCGTTGAGTGTGCAGAAGTTGGCGTGTCGACGTTGAGCAAAGTAGCCATGTCAAAGGGGATGAGCAAATTTGTCTTCATTGTCTACTACAATGCTCTTGGTACCCTCTTGCTCCTCCCCATCTTCATCTTCCAAAGAAACAGGCGAGCTCCTTTGACTTTCAGGCTCATCTGCCAATTCTTTCTCCTCGGCCTAATAGGGAGCGCAGGGAAAATACTGTTTTTCGCCGGTGTCAAAAACAGTTCGGCTCTCCTTGCGTCAGCAATGGCAAATCTCACCCCAATTTTCACTTTCTTGCTAGCCTTAATTTTCAGGATGGAAAAGCTAGACTTGAGAAAAGCAACGAGTTATGCCAAATCCTTGGGCGCCATTATATCAGTGGGAGGGGCATTGGTAGTGACCCTTTACAACGGCCCTGCCCTATTGAACTCTTCCTCTTCTTCCGGCTCGAGTCACTCAACTCATAGATCTTCCGTCTCACAGTCATCAACCTGGATGTTTGGAGGTCTGCTACTCGCAATCCAATGCCTCGTAGCTTCGGGATGGAACATCGCTCAGGCAGCCACTGTGAAGGATTACCCGGAAGAAATGACCATAGTCTTCTTCTACACCATGTTTCTTACAATTCAATGCACAGTTATCTCTCTAATTGTGGAAAGGAATAATCTGAATGCATGGAAACTGGAGCTTCCCATTGAGATAATCACCATTGTGTATGCGGCAATCTGTGTGAGTGTGTTTCGCATCAGTGTTCATGTCTGGTGCTTGCACAAGAAGGGGCCTGTTTATGTAGCCATGTTTTCACCCTTAGGAATCGCCATTGCGGCAGTCTTGGTGGTTGTTTTCCTTGGCGACACCTTTTATCTCGGCAGCTTGATTGGATCCATTGTGATCTCGGTGGGATTTTATACCGTGATATACGCAAAAATGAAGGAGAAAGCGATGGCGGATGATGAGGGAGTTCAACTGTCAAAACCGGCGGATGATGGGGAGTTCAACTATCAAAACCGGCGGATGATGTGGGAGTCCAACTATCAAAACCGTCTTCCCAAATCCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

371

Amino Acids

40.73

Weight (kDa)

9.43

Isoelectric Point (pI)

40.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000534)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g50400 FvH4_6g50410 FvH4_6g50410 FvH4_6g50410 FvH4_6g50410 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420
malus_domestica MD09G1036400.v1.1 MD09G1036600.v1.1 MD17G1038100.v1.1 MD17G1038200.v1.1
prunus_persica Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G281000_v2.0.a1 Prupe.3G281000_v2.0.a1 Prupe.3G281000_v2.0.a1 Prupe.3G281000_v2.0.a1
pyrus_communis pycom111g02920 pycom111g02930 pycom111g02940 pycom111g02950 pycom16g07600 pycom17g03430 pycom17g03440
rosa_chinensis RchiOBHm_Chr2g0170801 RchiOBHm_Chr2g0170811 RchiOBHm_Chr2g0170821 RchiOBHm_Chr2g0170831 RchiOBHm_Chr2g0170841
rosa_laevigata RLG00000021981 RLG00000021983 RLG00000021984 RLG00000021985
rosa_multiflora Rmu_co8418849.1_g000001 Rmu_co8437261.1_g000001 Rmu_sc0008393.1_g000003
rosa_roxburghii Rroxscaffold_1G00030380 Rroxscaffold_2G00081040 Rroxscaffold_2G00081050 Rroxscaffold_2G00081060
rosa_rugosa Rorug02G0551300 Rorug02G0551400 Rorug02G0551700 Rorug02G0551800
rosa_samantha Rh2AG624300 Rh2BG634700 Rh2BG634800 Rh2BG634900 Rh2BG635000 Rh2CG603500 Rh2CG603600 Rh2CG603700 Rh2CG603800
rosa_wichuraiana Rw0G017070 Rw2G051700 Rw2G051710 Rw2G051720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 152
AccB1I GGYRCC 3 cut(s) 26, 152, 416
AccI GTMKAC 2 cut(s) 80, 135
AciI CCGC 5 cut(s) 770, 875, 998, 1031, 1063
AclWI GGATC 2 cut(s) 927, 940
AcsI RAATTY 1 cut(s) 119
AcyI GRCGYC 2 cut(s) 27, 417
AfaI GTAC 1 cut(s) 154
AgsI TTSAA 4 cut(s) 478, 680, 1016, 1048
AjnI CCWGG 1 cut(s) 542
AleI CACNNNNGTG 1 cut(s) 761
AluBI AGCT 5 cut(s) 200, 376, 587, 739, 924
AluI AGCT 5 cut(s) 200, 376, 587, 739, 924
Alw21I GWGCWC 1 cut(s) 202
Alw26I GTCTC 1 cut(s) 532
AlwI GGATC 2 cut(s) 927, 940
AlwNI CAGNNNCTG 1 cut(s) 617
Ama87I CYCGRG 1 cut(s) 499
AoxI GGCC 3 cut(s) 241, 463, 830
ApeKI GCWGC 2 cut(s) 611, 921
ApoI RAATTY 1 cut(s) 119
Asp718I GGTACC 1 cut(s) 152
AspLEI GCGC 3 cut(s) 29, 257, 419
AspS9I GGNCC 2 cut(s) 464, 830
AsuC2I CCSGG 1 cut(s) 632
AsuHPI GGTGA 3 cut(s) 319, 748, 846
AsuNHI GCTAGC 1 cut(s) 349
AvaI CYCGRG 1 cut(s) 499
AxyI CCTNAGG 1 cut(s) 859
BaeGI GKGCMC 1 cut(s) 36
BamHI GGATCC 1 cut(s) 932
BanI GGYRCC 3 cut(s) 26, 152, 416
BanII GRGCYC 1 cut(s) 202
BbsI GAAGAC 3 cut(s) 118, 643, 1088
Bbv12I GWGCWC 1 cut(s) 202
BbvI GCAGC 2 cut(s) 623, 933
BccI CCATC 5 cut(s) 179, 361, 588, 988, 1031
BceAI ACGGC 2 cut(s) 14, 478
BciT130I CCWGG 1 cut(s) 544
BcnI CCSGG 1 cut(s) 632
BcoDI GTCTC 1 cut(s) 532
BfaI CTAG 2 cut(s) 350, 377
BfoI RGCGCY 2 cut(s) 30, 420
BglI GCCNNNNNGGC 1 cut(s) 875
BglII AGATCT 1 cut(s) 518
BisI GCNGC 4 cut(s) 612, 771, 876, 922
BlsI GCNGC 4 cut(s) 613, 772, 877, 923
Bme1390I CCNGG 2 cut(s) 544, 632
BmeT110I CYCGRG 1 cut(s) 499
BmgT120I GGNCC 2 cut(s) 464, 830
BmiI GGNNCC 5 cut(s) 28, 154, 418, 831, 934
BmrFI CCNGG 2 cut(s) 544, 632
BmsI GCATC 1 cut(s) 804
BmtI GCTAGC 1 cut(s) 353
BoxI GACNNNNGTC 1 cut(s) 647
BpiI GAAGAC 3 cut(s) 118, 643, 1088
BpmI CTGGAG 1 cut(s) 755
Bpu10I CCTNAGC 1 cut(s) 606
BpuEI CTTGAG 1 cut(s) 403
BpuMI CCSGG 1 cut(s) 632
BsaHI GRCGYC 2 cut(s) 27, 417
BsaJI CCNNGG 4 cut(s) 45, 238, 411, 898
Bse118I RCCGGY 3 cut(s) 278, 1027, 1059
Bse1I ACTGG 1 cut(s) 738
Bse21I CCTNAGG 1 cut(s) 859
Bse3DI GCAATG 2 cut(s) 321, 870
BseBI CCWGG 1 cut(s) 544
BseDI CCNNGG 4 cut(s) 45, 238, 411, 898
BseGI GGATG 7 cut(s) 117, 372, 552, 599, 1006, 1039, 1071
BseMI GCAATG 2 cut(s) 321, 870
BseMII CTCAG 1 cut(s) 620
BseNI ACTGG 1 cut(s) 738
BseRI GAGGAG 2 cut(s) 155, 227
BseSI GKGCMC 1 cut(s) 36
BseXI GCAGC 2 cut(s) 623, 933
BsgI GTGCAG 1 cut(s) 84
BshFI GGCC 3 cut(s) 243, 465, 832
BshNI GGYRCC 3 cut(s) 26, 152, 416
BsiHKAI GWGCWC 1 cut(s) 202
BsiHKCI CYCGRG 1 cut(s) 499
BsiSI CCGG 5 cut(s) 279, 495, 632, 1028, 1060
BsmAI GTCTC 1 cut(s) 532
BsmBI CGTCTC 1 cut(s) 532
BsmI GAATGC 1 cut(s) 727
BsnI GGCC 3 cut(s) 243, 465, 832
BsoBI CYCGRG 1 cut(s) 499
Bsp1286I GDGCHC 2 cut(s) 36, 202
Bsp143I GATC 3 cut(s) 518, 932, 942
Bsp19I CCATGG 1 cut(s) 45
BspACI CCGC 5 cut(s) 770, 875, 998, 1031, 1063
BspANI GGCC 3 cut(s) 243, 465, 832
BspCNI CTCAG 1 cut(s) 619
BspLI GGNNCC 5 cut(s) 28, 154, 418, 831, 934
BspOI GCTAGC 1 cut(s) 353
BspPI GGATC 2 cut(s) 927, 940
BspT107I GGYRCC 3 cut(s) 26, 152, 416
BsrDI GCAATG 2 cut(s) 321, 870
BsrFI RCCGGY 3 cut(s) 278, 1027, 1059
BsrI ACTGG 1 cut(s) 738
BssAI RCCGGY 3 cut(s) 278, 1027, 1059
BssECI CCNNGG 4 cut(s) 45, 238, 411, 898
BssMI GATC 3 cut(s) 518, 932, 942
BssNI GRCGYC 2 cut(s) 27, 417
BssT1I CCWWGG 3 cut(s) 45, 411, 898
Bst2UI CCWGG 1 cut(s) 544
Bst4CI ACNGT 8 cut(s) 270, 293, 534, 619, 688, 964, 1020, 1094
Bst6I CTCTTC 2 cut(s) 487, 493
BstACI GRCGYC 2 cut(s) 27, 417
BstC8I GCNNGC 3 cut(s) 198, 351, 818
BstDEI CTNAG 2 cut(s) 606, 859
BstDSI CCRYGG 1 cut(s) 45
BstF5I GGATG 7 cut(s) 117, 372, 552, 599, 1006, 1039, 1071
BstH2I RGCGCY 2 cut(s) 30, 420
BstHHI GCGC 3 cut(s) 29, 257, 419
BstKTI GATC 3 cut(s) 521, 935, 945
BstMAI GTCTC 1 cut(s) 532
BstMBI GATC 3 cut(s) 518, 932, 942
BstMWI GCNNNNNNNGC 4 cut(s) 24, 305, 611, 875
BstNI CCWGG 1 cut(s) 544
BstPAI GACNNNNGTC 1 cut(s) 647
BstSCI CCNGG 2 cut(s) 542, 630
BstSLI GKGCMC 1 cut(s) 36
BstV1I GCAGC 2 cut(s) 623, 933
BstV2I GAAGAC 3 cut(s) 118, 643, 1088
BstX2I RGATCY 2 cut(s) 518, 932
BstXI CCANNNNNNTGG 1 cut(s) 412
BstYI RGATCY 2 cut(s) 518, 932
Bsu36I CCTNAGG 1 cut(s) 859
BsuRI GGCC 3 cut(s) 243, 465, 832
BtgI CCRYGG 1 cut(s) 45
BtgZI GCGATG 2 cut(s) 586, 1007
BtsCI GGATG 7 cut(s) 117, 372, 552, 599, 1006, 1039, 1071
BtsI GCAGTG 1 cut(s) 39
BtsIMutI CAGTG 4 cut(s) 39, 435, 615, 805
Cac8I GCNNGC 3 cut(s) 198, 351, 818
CaiI CAGNNNCTG 1 cut(s) 617
CfoI GCGC 3 cut(s) 29, 257, 419
Cfr10I RCCGGY 3 cut(s) 278, 1027, 1059
Cfr13I GGNCC 2 cut(s) 464, 830
CseI GACGC 1 cut(s) 297
Csp6I GTAC 1 cut(s) 153
CviAII CATG 6 cut(s) 46, 100, 664, 726, 806, 847
CviQI GTAC 1 cut(s) 153
DdeI CTNAG 2 cut(s) 606, 859
DinI GGCGCC 2 cut(s) 28, 418
DpnI GATC 3 cut(s) 520, 934, 944
DpnII GATC 3 cut(s) 518, 932, 942
Eam1104I CTCTTC 2 cut(s) 487, 493
EarI CTCTTC 2 cut(s) 487, 493
EciI GGCGGA 3 cut(s) 1013, 1046, 1078
Ecl136II GAGCTC 1 cut(s) 200
Eco130I CCWWGG 3 cut(s) 45, 411, 898
Eco24I GRGCYC 1 cut(s) 202
Eco53kI GAGCTC 1 cut(s) 200
Eco81I CCTNAGG 1 cut(s) 859
Eco88I CYCGRG 1 cut(s) 499
EcoICRI GAGCTC 1 cut(s) 200
EcoO109I RGGNCCY 1 cut(s) 830
EcoRII CCWGG 1 cut(s) 542
EcoT14I CCWWGG 3 cut(s) 45, 411, 898
EcoT22I ATGCAT 1 cut(s) 727
EcoT38I GRGCYC 1 cut(s) 202
EgeI GGCGCC 2 cut(s) 28, 418
EheI GGCGCC 2 cut(s) 28, 418
ErhI CCWWGG 3 cut(s) 45, 411, 898
Esp3I CGTCTC 1 cut(s) 532
FaeI CATG 6 cut(s) 49, 103, 667, 729, 809, 850
FalI AAGNNNNNCTT 2 cut(s) 365, 397
FatI CATG 6 cut(s) 45, 99, 663, 725, 805, 846
FauNDI CATATG 1 cut(s) 20
FblI GTMKAC 2 cut(s) 80, 135
Fnu4HI GCNGC 4 cut(s) 612, 771, 876, 922
FokI GGATG 7 cut(s) 124, 379, 559, 606, 1013, 1046, 1078
FriOI GRGCYC 1 cut(s) 202
Fsp4HI GCNGC 4 cut(s) 612, 771, 876, 922
FspBI CTAG 2 cut(s) 350, 377
GlaI GCGC 3 cut(s) 28, 256, 418
GluI GCNGC 4 cut(s) 612, 771, 876, 922
GsuI CTGGAG 1 cut(s) 755
HaeII RGCGCY 2 cut(s) 30, 420
HaeIII GGCC 3 cut(s) 243, 465, 832
HapII CCGG 5 cut(s) 279, 495, 632, 1028, 1060
HgaI GACGC 1 cut(s) 297
HhaI GCGC 3 cut(s) 29, 257, 419
Hin1I GRCGYC 2 cut(s) 27, 417
Hin1II CATG 6 cut(s) 49, 103, 667, 729, 809, 850
Hin6I GCGC 3 cut(s) 27, 255, 417
HinP1I GCGC 3 cut(s) 27, 255, 417
HincII GTYRAC 1 cut(s) 81
HindII GTYRAC 1 cut(s) 81
HinfI GANTC 3 cut(s) 502, 864, 1076
HpaII CCGG 5 cut(s) 279, 495, 632, 1028, 1060
HphI GGTGA 3 cut(s) 319, 748, 846
Hpy166II GTNNAC 2 cut(s) 81, 136
Hpy188I TCNGA 1 cut(s) 720
Hpy188III TCNNGA 2 cut(s) 364, 591
Hpy8I GTNNAC 2 cut(s) 81, 136
Hpy99I CGWCG 1 cut(s) 85
HpyAV CCTTC 4 cut(s) 46, 616, 820, 976
HpyCH4III ACNGT 8 cut(s) 270, 293, 534, 619, 688, 964, 1020, 1094
HpyCH4IV ACGT 1 cut(s) 83
HpyCH4V TGCA 4 cut(s) 65, 684, 725, 820
HpyF10VI GCNNNNNNNGC 4 cut(s) 24, 305, 611, 875
HpyF3I CTNAG 2 cut(s) 606, 859
HpySE526I ACGT 1 cut(s) 83
Hsp92I GRCGYC 2 cut(s) 27, 417
Hsp92II CATG 6 cut(s) 49, 103, 667, 729, 809, 850
HspAI GCGC 3 cut(s) 27, 255, 417
KasI GGCGCC 2 cut(s) 26, 416
KpnI GGTACC 1 cut(s) 156
Kzo9I GATC 3 cut(s) 518, 932, 942
LmnI GCTCC 4 cut(s) 168, 205, 252, 736
Lsp1109I GCAGC 2 cut(s) 623, 933
LweI GCATC 1 cut(s) 804
MaeI CTAG 2 cut(s) 350, 377
MaeII ACGT 1 cut(s) 83
MaeIII GTNAC 2 cut(s) 448, 503
MalI GATC 3 cut(s) 520, 934, 944
MboI GATC 3 cut(s) 518, 932, 942
MflI RGATCY 2 cut(s) 518, 932
MhlI GDGCHC 2 cut(s) 36, 202
MluCI AATT 6 cut(s) 119, 227, 333, 357, 675, 699
Mly113I GGCGCC 2 cut(s) 27, 417
MlyI GAGTC 2 cut(s) 511, 1085
MmeI TCCRAC 1 cut(s) 1104
MnlI CCTC 8 cut(s) 167, 176, 248, 428, 496, 548, 590, 1000
Mph1103I ATGCAT 1 cut(s) 727
MseI TTAA 1 cut(s) 356
MslI CAYNNNNRTG 2 cut(s) 44, 761
MspI CCGG 5 cut(s) 279, 495, 632, 1028, 1060
MspR9I CCNGG 2 cut(s) 544, 632
Mva1269I GAATGC 1 cut(s) 727
MvaI CCWGG 1 cut(s) 544
MwoI GCNNNNNNNGC 4 cut(s) 24, 305, 611, 875
NarI GGCGCC 2 cut(s) 27, 417
NciI CCSGG 1 cut(s) 632
NcoI CCATGG 1 cut(s) 45
NdeI CATATG 1 cut(s) 20
NdeII GATC 3 cut(s) 518, 932, 942
NheI GCTAGC 1 cut(s) 349
NlaIII CATG 6 cut(s) 49, 103, 667, 729, 809, 850
NlaIV GGNNCC 5 cut(s) 28, 154, 418, 831, 934
NmeAIII GCCGAG 2 cut(s) 219, 897
NmuCI GTSAC 2 cut(s) 448, 503
NsiI ATGCAT 1 cut(s) 727
OliI CACNNNNGTG 1 cut(s) 761
PaeR7I CTCGAG 1 cut(s) 499
PctI GAATGC 1 cut(s) 727
PfeI GAWTC 1 cut(s) 864
PkrI GCNGC 4 cut(s) 613, 772, 877, 923
PleI GAGTC 2 cut(s) 510, 1084
PluTI GGCGCC 2 cut(s) 30, 420
PpsI GAGTC 2 cut(s) 510, 1084
PshAI GACNNNNGTC 1 cut(s) 647
Psp124BI GAGCTC 1 cut(s) 202
Psp6I CCWGG 1 cut(s) 542
PspGI CCWGG 1 cut(s) 542
PspN4I GGNNCC 5 cut(s) 28, 154, 418, 831, 934
PspPI GGNCC 2 cut(s) 464, 830
PspXI VCTCGAGB 1 cut(s) 499
PstNI CAGNNNCTG 1 cut(s) 617
PsuI RGATCY 2 cut(s) 518, 932
RsaI GTAC 1 cut(s) 154
RsaNI GTAC 1 cut(s) 153
RseI CAYNNNNRTG 2 cut(s) 44, 761
SacI GAGCTC 1 cut(s) 202
SalI GTCGAC 1 cut(s) 79
SaqAI TTAA 1 cut(s) 356
SatI GCNGC 4 cut(s) 612, 771, 876, 922
Sau3AI GATC 3 cut(s) 518, 932, 942
Sau96I GGNCC 2 cut(s) 464, 830
SchI GAGTC 2 cut(s) 511, 1085
ScrFI CCNGG 2 cut(s) 544, 632
SduI GDGCHC 2 cut(s) 36, 202
SetI ASST 9 cut(s) 86, 202, 378, 545, 559, 589, 741, 911, 926
SfaNI GCATC 1 cut(s) 804
SfoI GGCGCC 2 cut(s) 28, 418
Sfr274I CTCGAG 1 cut(s) 499
SgrAI CRCCGGYG 1 cut(s) 278
SlaI CTCGAG 1 cut(s) 499
SmiMI CAYNNNNRTG 2 cut(s) 44, 761
SmlI CTYRAG 2 cut(s) 382, 499
SmoI CTYRAG 2 cut(s) 382, 499
Sse9I AATT 6 cut(s) 119, 227, 333, 357, 675, 699
SsiI CCGC 5 cut(s) 770, 875, 998, 1031, 1063
SspDI GGCGCC 2 cut(s) 26, 416
SspMI CTAG 2 cut(s) 350, 377
SstI GAGCTC 1 cut(s) 202
StyD4I CCNGG 2 cut(s) 542, 630
StyI CCWWGG 3 cut(s) 45, 411, 898
TaaI ACNGT 8 cut(s) 270, 293, 534, 619, 688, 964, 1020, 1094
TaiI ACGT 1 cut(s) 86
TaqI TCGA 2 cut(s) 80, 500
TasI AATT 6 cut(s) 119, 227, 333, 357, 675, 699
TauI GCSGC 2 cut(s) 773, 878
TfiI GAWTC 1 cut(s) 864
Tru1I TTAA 1 cut(s) 356
Tru9I TTAA 1 cut(s) 356
TscAI CASTG 4 cut(s) 46, 435, 622, 805
TseFI GTSAC 2 cut(s) 448, 503
TseI GCWGC 2 cut(s) 611, 921
Tsp45I GTSAC 2 cut(s) 448, 503
TspDTI ATGAA 4 cut(s) 118, 166, 794, 995
TspGWI ACGGA 1 cut(s) 514
TspRI CASTG 4 cut(s) 46, 435, 622, 805
XapI RAATTY 1 cut(s) 119
XcmI CCANNNNNNNNNTGG 1 cut(s) 427
XhoI CTCGAG 1 cut(s) 499
XmiI GTMKAC 2 cut(s) 80, 135
XspI CTAG 2 cut(s) 350, 377
Zsp2I ATGCAT 1 cut(s) 727
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.