MD09G1036400.v1.1

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000534
Physical Location & Seq
Forward (+)
2247951 .. 2250011
2061 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1036400.v1.1.491

Sequence Viewer

Length: 1080 bp
ATGGATATGCAGGTAGGTTGCAGGTGGGGTGTTTTGTTACCTGTTGTAGCTATGGTGACGGTGGAGTTCACAGACGTCGGCGTAACGACTGTAAGCAAAGCAGCGATGTCAAGAGGAATGAGCAGTTATGTTTTTGTTGTTTACTCTAACGCCCTCGCTGCCATCTTTCTCCTTCCTTGCTTCATTTTACAAAAGAAGCCGGCCACTTCTCTTCCTCTTTCGCTCCTTTGCGGACTCTTCCTCCTTGGCCTAATTGGGAGTTCGAGCTTGATATTAGCATATAATGGCATCAACTACAGCTCACCAACAGTTTCATCAGCAATGGGAAACCTCATACCAATTTTTACCTTCGCACTGGCAGTCATTTTTAGGATTGAAAAGCCGAATTTAAGGAAATCAAGCACTCGAGCCAAATTGTTGGGCACTATTGTATCGGTATCTGGTGCTTTCATAGTGATCCTTTATAAGGGCTCGGCAATCTTAAAGTTCCAGGGTTTATCACCACCAAACTTTTCTTACCAGCACTTCGTGTCGCAGCGGACAAATTGGGTCTTTGGGGGTTTTCTCCTTGCTCTGTCTTGTCTTTTGGCTGCAATATGGAACATTGTGCAGGCACCCATTGTAAACAACTTTCCATCAAAGGAGACCATAGTATTCTTCTACATCTTTTTCGTCACAATCCAAACTACAATATATTCCCTAATTGTGGAAAGGAATCCAAATTCTTGGGTATTAAGGCCCGACATCGAGATGATCGCCATTGTCTGCTCCGCTATGTTTGGGAGTGTCTTTCGTACCGCTGTTCATGTTTGGTGCTTGCAGCAAAACGGGCCAATCTTTGTAACCATGTTCAGGCCGTTGGGAGTTGCCATTGCAGCTGCCATGGTGGTCATCTTCCTCGGCGAGTCACTTCACCTTGGCAGTGTAATTGGATCAATCATTATTGCTATTGGATTTTATGCTATGATATGGGGACAAATCAAAGAGCATAAAATCGCCATGGAGAATGAAGTCCACAGCTCGGCATCAGCCACCCAACAGGCTCCACTTTTGCCGTGCAGAGTGGCGGAAGATCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

360

Amino Acids

39.15

Weight (kDa)

9.2

Isoelectric Point (pI)

33.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 22 - 153 6.9e-11 EamA-like transporter family
EamA PF00892 185 - 322 9.1e-06 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000534)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g50400 FvH4_6g50410 FvH4_6g50410 FvH4_6g50410 FvH4_6g50410 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420 FvH4_6g50420
malus_domestica MD09G1036400.v1.1 MD09G1036600.v1.1 MD17G1038100.v1.1 MD17G1038200.v1.1
prunus_persica Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G280900_v2.0.a1 Prupe.3G281000_v2.0.a1 Prupe.3G281000_v2.0.a1 Prupe.3G281000_v2.0.a1 Prupe.3G281000_v2.0.a1
pyrus_communis pycom111g02920 pycom111g02930 pycom111g02940 pycom111g02950 pycom16g07600 pycom17g03430 pycom17g03440
rosa_chinensis RchiOBHm_Chr2g0170801 RchiOBHm_Chr2g0170811 RchiOBHm_Chr2g0170821 RchiOBHm_Chr2g0170831 RchiOBHm_Chr2g0170841
rosa_laevigata RLG00000021981 RLG00000021983 RLG00000021984 RLG00000021985
rosa_multiflora Rmu_co8418849.1_g000001 Rmu_co8437261.1_g000001 Rmu_sc0008393.1_g000003
rosa_roxburghii Rroxscaffold_1G00030380 Rroxscaffold_2G00081040 Rroxscaffold_2G00081050 Rroxscaffold_2G00081060
rosa_rugosa Rorug02G0551300 Rorug02G0551400 Rorug02G0551700 Rorug02G0551800
rosa_samantha Rh2AG624300 Rh2BG634700 Rh2BG634800 Rh2BG634900 Rh2BG635000 Rh2CG603500 Rh2CG603600 Rh2CG603700 Rh2CG603800
rosa_wichuraiana Rw0G017070 Rw2G051700 Rw2G051710 Rw2G051720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 465
AarI CACCTGC 1 cut(s) 12
AatII GACGTC 1 cut(s) 78
Acc36I ACCTGC 1 cut(s) 12
AccB1I GGYRCC 1 cut(s) 613
AciI CCGC 5 cut(s) 231, 538, 771, 798, 1067
AclWI GGATC 2 cut(s) 451, 940
AcoI YGGCCR 1 cut(s) 201
AcsI RAATTY 2 cut(s) 385, 721
AcyI GRCGYC 1 cut(s) 75
AdeI CACNNNGTG 1 cut(s) 529
AfaI GTAC 1 cut(s) 796
AfiI CCNNNNNNNGG 4 cut(s) 466, 706, 852, 1021
AgsI TTSAA 1 cut(s) 377
AjnI CCWGG 1 cut(s) 489
AluBI AGCT 5 cut(s) 50, 267, 300, 878, 1020
AluI AGCT 5 cut(s) 50, 267, 300, 878, 1020
Alw26I GTCTC 1 cut(s) 638
AlwI GGATC 2 cut(s) 451, 940
Ama87I CYCGRG 1 cut(s) 405
AoxI GGCC 5 cut(s) 201, 247, 737, 830, 854
ApeKI GCWGC 7 cut(s) 101, 158, 535, 590, 820, 875, 878
ApoI RAATTY 2 cut(s) 385, 721
AspS9I GGNCC 2 cut(s) 738, 830
AsuHPI GGTGA 4 cut(s) 67, 294, 492, 905
AvaI CYCGRG 1 cut(s) 405
BaeGI GKGCMC 1 cut(s) 425
BaeI ACNNNNGTAYC 2 cut(s) 414, 447
BanI GGYRCC 1 cut(s) 613
BanII GRGCYC 1 cut(s) 473
BbvI GCAGC 7 cut(s) 113, 145, 547, 577, 832, 865, 887
BccI CCATC 2 cut(s) 170, 643
BceAI ACGGC 2 cut(s) 841, 1039
BciT130I CCWGG 1 cut(s) 491
BcoDI GTCTC 1 cut(s) 638
BfmI CTRYAG 1 cut(s) 295
BfuAI ACCTGC 1 cut(s) 12
BisI GCNGC 7 cut(s) 102, 159, 536, 591, 821, 876, 879
BlsI GCNGC 7 cut(s) 103, 160, 537, 592, 822, 877, 880
Bme1390I CCNGG 1 cut(s) 491
BmeT110I CYCGRG 1 cut(s) 405
BmgT120I GGNCC 2 cut(s) 738, 830
BmiI GGNNCC 2 cut(s) 615, 1044
BmrFI CCNGG 1 cut(s) 491
BmsI GCATC 2 cut(s) 297, 1034
BsaHI GRCGYC 1 cut(s) 75
BsaI GGTCTC 1 cut(s) 638
BsaJI CCNNGG 6 cut(s) 244, 490, 882, 898, 916, 999
BsaXI ACNNNNNCTCC 2 cut(s) 225, 255
Bsc4I CCNNNNNNNGG 4 cut(s) 466, 706, 852, 1021
Bse118I RCCGGY 1 cut(s) 199
Bse1I ACTGG 1 cut(s) 360
Bse3DI GCAATG 2 cut(s) 327, 870
BseBI CCWGG 1 cut(s) 491
BseDI CCNNGG 6 cut(s) 244, 490, 882, 898, 916, 999
BseLI CCNNNNNNNGG 4 cut(s) 466, 706, 852, 1021
BseMI GCAATG 2 cut(s) 327, 870
BseNI ACTGG 1 cut(s) 360
BseSI GKGCMC 1 cut(s) 425
BseXI GCAGC 7 cut(s) 113, 145, 547, 577, 832, 865, 887
BsgI GTGCAG 2 cut(s) 629, 1078
BshFI GGCC 5 cut(s) 203, 249, 739, 832, 856
BshNI GGYRCC 1 cut(s) 613
BsiHKCI CYCGRG 1 cut(s) 405
BsiSI CCGG 1 cut(s) 200
BslFI GGGAC 1 cut(s) 987
BslI CCNNNNNNNGG 4 cut(s) 466, 706, 852, 1021
BsmAI GTCTC 1 cut(s) 638
BsmFI GGGAC 1 cut(s) 987
BsnI GGCC 5 cut(s) 203, 249, 739, 832, 856
Bso31I GGTCTC 1 cut(s) 638
BsoBI CYCGRG 1 cut(s) 405
Bsp1286I GDGCHC 2 cut(s) 425, 473
Bsp143I GATC 4 cut(s) 456, 753, 932, 1072
Bsp19I CCATGG 2 cut(s) 882, 999
BspACI CCGC 5 cut(s) 231, 538, 771, 798, 1067
BspANI GGCC 5 cut(s) 203, 249, 739, 832, 856
BspLI GGNNCC 2 cut(s) 615, 1044
BspMI ACCTGC 1 cut(s) 12
BspPI GGATC 2 cut(s) 451, 940
BspT107I GGYRCC 1 cut(s) 613
BspTNI GGTCTC 1 cut(s) 638
BsrDI GCAATG 2 cut(s) 327, 870
BsrFI RCCGGY 1 cut(s) 199
BsrI ACTGG 1 cut(s) 360
BssAI RCCGGY 1 cut(s) 199
BssECI CCNNGG 6 cut(s) 244, 490, 882, 898, 916, 999
BssMI GATC 4 cut(s) 456, 753, 932, 1072
BssNI GRCGYC 1 cut(s) 75
BssT1I CCWWGG 4 cut(s) 244, 882, 916, 999
Bst2UI CCWGG 1 cut(s) 491
Bst4CI ACNGT 3 cut(s) 61, 91, 310
Bst6I CTCTTC 2 cut(s) 216, 242
BstACI GRCGYC 1 cut(s) 75
BstC8I GCNNGC 3 cut(s) 201, 612, 818
BstDSI CCRYGG 2 cut(s) 882, 999
BstENI CCTNNNNNAGG 1 cut(s) 464
BstKTI GATC 4 cut(s) 459, 756, 935, 1075
BstMAI GTCTC 1 cut(s) 638
BstMBI GATC 4 cut(s) 456, 753, 932, 1072
BstMWI GCNNNNNNNGC 3 cut(s) 158, 829, 875
BstNI CCWGG 1 cut(s) 491
BstSCI CCNGG 1 cut(s) 489
BstSFI CTRYAG 1 cut(s) 295
BstSLI GKGCMC 1 cut(s) 425
BstV1I GCAGC 7 cut(s) 113, 145, 547, 577, 832, 865, 887
BstXI CCANNNNNNTGG 2 cut(s) 418, 726
BsuRI GGCC 5 cut(s) 203, 249, 739, 832, 856
BtgI CCRYGG 2 cut(s) 882, 999
BtgZI GCGATG 1 cut(s) 119
BtsI GCAGTG 1 cut(s) 928
BtsIMutI CAGTG 2 cut(s) 353, 928
BveI ACCTGC 1 cut(s) 12
Cac8I GCNNGC 3 cut(s) 201, 612, 818
Cfr10I RCCGGY 1 cut(s) 199
Cfr13I GGNCC 2 cut(s) 738, 830
Csp6I GTAC 1 cut(s) 795
CviAII CATG 4 cut(s) 806, 847, 883, 1000
CviQI GTAC 1 cut(s) 795
DpnI GATC 4 cut(s) 458, 755, 934, 1074
DpnII GATC 4 cut(s) 456, 753, 932, 1072
DraIII CACNNNGTG 1 cut(s) 529
EaeI YGGCCR 1 cut(s) 201
Eam1104I CTCTTC 2 cut(s) 216, 242
EarI CTCTTC 2 cut(s) 216, 242
Eco130I CCWWGG 4 cut(s) 244, 882, 916, 999
Eco24I GRGCYC 1 cut(s) 473
Eco31I GGTCTC 1 cut(s) 638
Eco88I CYCGRG 1 cut(s) 405
EcoNI CCTNNNNNAGG 1 cut(s) 464
EcoRII CCWGG 1 cut(s) 489
EcoT14I CCWWGG 4 cut(s) 244, 882, 916, 999
EcoT38I GRGCYC 1 cut(s) 473
ErhI CCWWGG 4 cut(s) 244, 882, 916, 999
FaeI CATG 4 cut(s) 809, 850, 886, 1003
FaqI GGGAC 1 cut(s) 987
FatI CATG 4 cut(s) 805, 846, 882, 999
Fnu4HI GCNGC 7 cut(s) 102, 159, 536, 591, 821, 876, 879
FriOI GRGCYC 1 cut(s) 473
Fsp4HI GCNGC 7 cut(s) 102, 159, 536, 591, 821, 876, 879
GluI GCNGC 7 cut(s) 102, 159, 536, 591, 821, 876, 879
HaeIII GGCC 5 cut(s) 203, 249, 739, 832, 856
HapII CCGG 1 cut(s) 200
Hin1I GRCGYC 1 cut(s) 75
Hin1II CATG 4 cut(s) 809, 850, 886, 1003
HinfI GANTC 3 cut(s) 234, 715, 905
HpaII CCGG 1 cut(s) 200
HphI GGTGA 4 cut(s) 67, 294, 492, 905
Hpy166II GTNNAC 4 cut(s) 69, 142, 625, 1015
Hpy188III TCNNGA 2 cut(s) 111, 748
Hpy8I GTNNAC 4 cut(s) 69, 142, 625, 1015
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 2 cut(s) 182, 358
HpyCH4III ACNGT 3 cut(s) 61, 91, 310
HpyCH4IV ACGT 1 cut(s) 75
HpyCH4V TGCA 7 cut(s) 10, 21, 593, 610, 820, 875, 1059
HpyF10VI GCNNNNNNNGC 3 cut(s) 158, 829, 875
HpySE526I ACGT 1 cut(s) 75
Hsp92I GRCGYC 1 cut(s) 75
Hsp92II CATG 4 cut(s) 809, 850, 886, 1003
KroI GCCGGC 1 cut(s) 199
KroNI GCCGGC 1 cut(s) 201
Kzo9I GATC 4 cut(s) 456, 753, 932, 1072
LmnI GCTCC 3 cut(s) 228, 773, 1048
Lsp1109I GCAGC 7 cut(s) 113, 145, 547, 577, 832, 865, 887
LweI GCATC 2 cut(s) 297, 1034
MaeII ACGT 1 cut(s) 75
MaeIII GTNAC 6 cut(s) 36, 55, 82, 673, 841, 906
MalI GATC 4 cut(s) 458, 755, 934, 1074
MboI GATC 4 cut(s) 456, 753, 932, 1072
MboII GAAGA 4 cut(s) 203, 229, 649, 886
MhlI GDGCHC 2 cut(s) 425, 473
MluCI AATT 8 cut(s) 252, 339, 385, 413, 544, 702, 721, 927
MlyI GAGTC 2 cut(s) 228, 914
MnlI CCTC 6 cut(s) 107, 164, 225, 251, 341, 908
MroNI GCCGGC 1 cut(s) 199
MseI TTAA 3 cut(s) 389, 482, 734
MslI CAYNNNNRTG 1 cut(s) 749
MspA1I CMGCKG 3 cut(s) 538, 800, 878
MspI CCGG 1 cut(s) 200
MspR9I CCNGG 1 cut(s) 491
MvaI CCWGG 1 cut(s) 491
MwoI GCNNNNNNNGC 3 cut(s) 158, 829, 875
NaeI GCCGGC 1 cut(s) 201
NcoI CCATGG 2 cut(s) 882, 999
NdeII GATC 4 cut(s) 456, 753, 932, 1072
NgoMIV GCCGGC 1 cut(s) 199
NlaIII CATG 4 cut(s) 809, 850, 886, 1003
NlaIV GGNNCC 2 cut(s) 615, 1044
NmeAIII GCCGAG 3 cut(s) 452, 879, 1001
NmuCI GTSAC 3 cut(s) 55, 673, 906
PaeR7I CTCGAG 1 cut(s) 405
PaqCI CACCTGC 1 cut(s) 12
PdiI GCCGGC 1 cut(s) 201
PfeI GAWTC 1 cut(s) 715
PkrI GCNGC 7 cut(s) 103, 160, 537, 592, 822, 877, 880
PleI GAGTC 2 cut(s) 228, 913
PpsI GAGTC 2 cut(s) 228, 913
PsiI TTATAA 1 cut(s) 465
Psp6I CCWGG 1 cut(s) 489
PspGI CCWGG 1 cut(s) 489
PspN4I GGNNCC 2 cut(s) 615, 1044
PspPI GGNCC 2 cut(s) 738, 830
PspXI VCTCGAGB 1 cut(s) 405
PvuII CAGCTG 1 cut(s) 878
RsaI GTAC 1 cut(s) 796
RsaNI GTAC 1 cut(s) 795
RseI CAYNNNNRTG 1 cut(s) 749
SaqAI TTAA 3 cut(s) 389, 482, 734
SatI GCNGC 7 cut(s) 102, 159, 536, 591, 821, 876, 879
Sau3AI GATC 4 cut(s) 456, 753, 932, 1072
Sau96I GGNCC 2 cut(s) 738, 830
SchI GAGTC 2 cut(s) 228, 914
ScrFI CCNGG 1 cut(s) 491
SduI GDGCHC 2 cut(s) 425, 473
SfaNI GCATC 2 cut(s) 297, 1034
SfcI CTRYAG 1 cut(s) 295
Sfr274I CTCGAG 1 cut(s) 405
SlaI CTCGAG 1 cut(s) 405
SmiMI CAYNNNNRTG 1 cut(s) 749
SmlI CTYRAG 1 cut(s) 405
SmoI CTYRAG 1 cut(s) 405
Sse9I AATT 8 cut(s) 252, 339, 385, 413, 544, 702, 721, 927
SsiI CCGC 5 cut(s) 231, 538, 771, 798, 1067
StyD4I CCNGG 1 cut(s) 489
StyI CCWWGG 4 cut(s) 244, 882, 916, 999
TaaI ACNGT 3 cut(s) 61, 91, 310
TaiI ACGT 1 cut(s) 78
TaqI TCGA 3 cut(s) 263, 406, 747
TasI AATT 8 cut(s) 252, 339, 385, 413, 544, 702, 721, 927
TfiI GAWTC 1 cut(s) 715
Tru1I TTAA 3 cut(s) 389, 482, 734
Tru9I TTAA 3 cut(s) 389, 482, 734
TscAI CASTG 2 cut(s) 360, 928
TseFI GTSAC 3 cut(s) 55, 673, 906
TseI GCWGC 7 cut(s) 101, 158, 535, 590, 820, 875, 878
Tsp45I GTSAC 3 cut(s) 55, 673, 906
TspDTI ATGAA 5 cut(s) 172, 303, 439, 794, 1023
TspRI CASTG 2 cut(s) 360, 928
XagI CCTNNNNNAGG 1 cut(s) 464
XapI RAATTY 2 cut(s) 385, 721
XhoI CTCGAG 1 cut(s) 405
ZraI GACGTC 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.