MD02G1209600.v1.1

Vesicle-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001420
Physical Location & Seq
Forward (+)
21489460 .. 21493214
3755 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1209600.v1.1.491

Sequence Viewer

Length: 717 bp
ATGAGTACCGGAGATCTTCTTAGCATTCAGCCCTTGGAGCTCAAATTCCCATTTGAAGTGAAGAAACACAGTTCATGTTCCTTGCAATTGACCAACAAAACAGCTAATTATGTGGCCTTCAAGGTTAAAACAACCAATCCGAAAAAATACTGTGTCCGGCCCAATGCTGGTGTTGTTTTGCCCGGAACCACATGCGATGTTACAGTCACAATGCAAGCCCAAAAAGAGGCTCCGCCAGACATGCAGTGCAAGGACAAATTTCTCCTTCAAAGTGTTGTTGCACCAGAAGGTGCAACTACGAAAGACATAACTCCTGAAATGTTCAACAAGGAGGATGGTAAAGTTGTGGAAGAGTTCAAATTGCGGGTAATCTACTTTCCTGCCAATCCCCCATCACCTGTCCCTGAAGGATCTGACGAGGGCTCTTCTCCAAAGGCTTCAGTCGTTGATAATGGAAATCAAAACTCTTCCTTGTTTGATGCTGTATCAAGGTCTCTTGAGGAACCAAAAGAGAAGTCGTCAGAGGCATGGTCCATGATTTCCAAGTTAACTGAAGAGAAGACTGCCGCTTTTCAACAAACTCAGAAGCTACGCCACGAACTGGTTGTGTTGAGAAAAGAAATTAGCAAAAACAGCAGTGGCAGATTTTCGTTGTTGTTTGTGGTGCTGTTCGGTCTTCTGGGCATCTTGGTTGGCTACTTCATCAACAAAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

239

Amino Acids

26.27

Weight (kDa)

8.56

Isoelectric Point (pI)

46.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 7 - 110 1.6e-32 MSP (Major sperm protein) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 601
AciI CCGC 3 cut(s) 233, 364, 567
AclWI GGATC 1 cut(s) 418
AcsI RAATTY 2 cut(s) 44, 257
AcuI CTGAAG 3 cut(s) 423, 426, 573
AfaI GTAC 1 cut(s) 7
AfiI CCNNNNNNNGG 3 cut(s) 167, 226, 601
AgsI TTSAA 6 cut(s) 56, 121, 269, 325, 358, 575
AluBI AGCT 3 cut(s) 40, 104, 589
AluI AGCT 3 cut(s) 40, 104, 589
Alw21I GWGCWC 1 cut(s) 42
Alw26I GTCTC 1 cut(s) 498
AlwI GGATC 1 cut(s) 418
AoxI GGCC 2 cut(s) 114, 158
ApoI RAATTY 2 cut(s) 44, 257
ArsI GACNNNNNNTTYG 2 cut(s) 500, 532
AspS9I GGNCC 2 cut(s) 159, 531
AsuC2I CCSGG 1 cut(s) 183
AsuHPI GGTGA 1 cut(s) 387
AvaII GGWCC 1 cut(s) 531
BanII GRGCYC 2 cut(s) 42, 425
BbsI GAAGAC 2 cut(s) 566, 668
Bbv12I GWGCWC 1 cut(s) 42
BccI CCATC 2 cut(s) 329, 400
BcnI CCSGG 1 cut(s) 183
BcoDI GTCTC 1 cut(s) 498
BglII AGATCT 1 cut(s) 13
BisI GCNGC 1 cut(s) 567
BlsI GCNGC 1 cut(s) 568
Bme1390I CCNGG 1 cut(s) 183
Bme18I GGWCC 1 cut(s) 531
BmgT120I GGNCC 2 cut(s) 159, 531
BmiI GGNNCC 3 cut(s) 187, 231, 504
BmrFI CCNGG 1 cut(s) 183
BmsI GCATC 2 cut(s) 469, 693
BpiI GAAGAC 2 cut(s) 566, 668
BpuEI CTTGAG 1 cut(s) 518
BpuMI CCSGG 1 cut(s) 183
BsaI GGTCTC 1 cut(s) 498
BsaJI CCNNGG 1 cut(s) 33
BsaWI WCCGGW 1 cut(s) 8
Bsc4I CCNNNNNNNGG 3 cut(s) 167, 226, 601
Bse1I ACTGG 1 cut(s) 606
BseDI CCNNGG 1 cut(s) 33
BseGI GGATG 1 cut(s) 340
BseLI CCNNNNNNNGG 3 cut(s) 167, 226, 601
BseMII CTCAG 1 cut(s) 596
BseNI ACTGG 1 cut(s) 606
BshFI GGCC 2 cut(s) 116, 160
BsiHKAI GWGCWC 1 cut(s) 42
BsiSI CCGG 3 cut(s) 9, 157, 183
BslFI GGGAC 1 cut(s) 386
BslI CCNNNNNNNGG 3 cut(s) 167, 226, 601
BsmAI GTCTC 1 cut(s) 498
BsmFI GGGAC 1 cut(s) 386
BsmI GAATGC 1 cut(s) 24
BsnI GGCC 2 cut(s) 116, 160
Bso31I GGTCTC 1 cut(s) 498
Bsp1286I GDGCHC 2 cut(s) 42, 425
Bsp143I GATC 2 cut(s) 13, 410
BspACI CCGC 3 cut(s) 233, 364, 567
BspANI GGCC 2 cut(s) 116, 160
BspCNI CTCAG 1 cut(s) 595
BspLI GGNNCC 3 cut(s) 187, 231, 504
BspPI GGATC 1 cut(s) 418
BspQI GCTCTTC 1 cut(s) 430
BspTNI GGTCTC 1 cut(s) 498
BsrI ACTGG 1 cut(s) 606
BssECI CCNNGG 1 cut(s) 33
BssMI GATC 2 cut(s) 13, 410
BssT1I CCWWGG 1 cut(s) 33
Bst4CI ACNGT 3 cut(s) 71, 152, 205
Bst6I CTCTTC 4 cut(s) 345, 430, 472, 549
BstC8I GCNNGC 1 cut(s) 216
BstDEI CTNAG 2 cut(s) 20, 582
BstF5I GGATG 1 cut(s) 340
BstKTI GATC 2 cut(s) 16, 413
BstMAI GTCTC 1 cut(s) 498
BstMBI GATC 2 cut(s) 13, 410
BstMWI GCNNNNNNNGC 3 cut(s) 37, 241, 633
BstNSI RCATGY 2 cut(s) 195, 244
BstSCI CCNGG 1 cut(s) 181
BstV2I GAAGAC 2 cut(s) 566, 668
BstX2I RGATCY 2 cut(s) 13, 410
BstYI RGATCY 2 cut(s) 13, 410
BsuRI GGCC 2 cut(s) 116, 160
BtgZI GCGATG 1 cut(s) 210
BtsCI GGATG 1 cut(s) 340
BtsI GCAGTG 2 cut(s) 251, 643
BtsIMutI CAGTG 2 cut(s) 251, 643
Cac8I GCNNGC 1 cut(s) 216
Cfr13I GGNCC 2 cut(s) 159, 531
Csp6I GTAC 1 cut(s) 6
CviAII CATG 5 cut(s) 75, 192, 241, 528, 535
CviQI GTAC 1 cut(s) 6
DdeI CTNAG 2 cut(s) 20, 582
DpnI GATC 2 cut(s) 15, 412
DpnII GATC 2 cut(s) 13, 410
Eam1104I CTCTTC 4 cut(s) 345, 430, 472, 549
EarI CTCTTC 4 cut(s) 345, 430, 472, 549
EciI GGCGGA 1 cut(s) 222
Ecl136II GAGCTC 1 cut(s) 40
Eco130I CCWWGG 1 cut(s) 33
Eco24I GRGCYC 2 cut(s) 42, 425
Eco31I GGTCTC 1 cut(s) 498
Eco47I GGWCC 1 cut(s) 531
Eco53kI GAGCTC 1 cut(s) 40
Eco57I CTGAAG 3 cut(s) 423, 426, 573
EcoICRI GAGCTC 1 cut(s) 40
EcoT14I CCWWGG 1 cut(s) 33
EcoT38I GRGCYC 2 cut(s) 42, 425
ErhI CCWWGG 1 cut(s) 33
FaeI CATG 5 cut(s) 78, 195, 244, 531, 538
FaiI YATR 8 cut(s) 76, 111, 193, 242, 308, 529, 536, 715
FaqI GGGAC 1 cut(s) 386
FatI CATG 5 cut(s) 74, 191, 240, 527, 534
FauI CCCGC 1 cut(s) 357
Fnu4HI GCNGC 1 cut(s) 567
FokI GGATG 1 cut(s) 347
FriOI GRGCYC 2 cut(s) 42, 425
Fsp4HI GCNGC 1 cut(s) 567
GluI GCNGC 1 cut(s) 567
HaeIII GGCC 2 cut(s) 116, 160
HapII CCGG 3 cut(s) 9, 157, 183
Hin1II CATG 5 cut(s) 78, 195, 244, 531, 538
HincII GTYRAC 1 cut(s) 549
HindII GTYRAC 1 cut(s) 549
HpaI GTTAAC 1 cut(s) 549
HpaII CCGG 3 cut(s) 9, 157, 183
HphI GGTGA 1 cut(s) 387
Hpy166II GTNNAC 1 cut(s) 549
Hpy188I TCNGA 4 cut(s) 141, 415, 523, 585
Hpy188III TCNNGA 2 cut(s) 314, 497
Hpy8I GTNNAC 1 cut(s) 549
HpyAV CCTTC 4 cut(s) 127, 275, 281, 401
HpyCH4III ACNGT 3 cut(s) 71, 152, 205
HpyCH4V TGCA 6 cut(s) 85, 214, 244, 249, 281, 293
HpyF10VI GCNNNNNNNGC 3 cut(s) 37, 241, 633
HpyF3I CTNAG 2 cut(s) 20, 582
Hsp92II CATG 5 cut(s) 78, 195, 244, 531, 538
KspAI GTTAAC 1 cut(s) 549
Kzo9I GATC 2 cut(s) 13, 410
LguI GCTCTTC 1 cut(s) 430
LmnI GCTCC 2 cut(s) 37, 235
LweI GCATC 2 cut(s) 469, 693
MaeIII GTNAC 2 cut(s) 199, 205
MalI GATC 2 cut(s) 15, 412
MboI GATC 2 cut(s) 13, 410
MboII GAAGA 8 cut(s) 8, 73, 362, 417, 459, 566, 571, 668
MfeI CAATTG 1 cut(s) 86
MflI RGATCY 2 cut(s) 13, 410
MhlI GDGCHC 2 cut(s) 42, 425
MluCI AATT 6 cut(s) 44, 86, 106, 257, 359, 621
MnlI CCTC 5 cut(s) 220, 325, 412, 493, 517
MseI TTAA 2 cut(s) 126, 548
MspI CCGG 3 cut(s) 9, 157, 183
MspR9I CCNGG 1 cut(s) 183
MunI CAATTG 1 cut(s) 86
Mva1269I GAATGC 1 cut(s) 24
MwoI GCNNNNNNNGC 3 cut(s) 37, 241, 633
NciI CCSGG 1 cut(s) 183
NdeII GATC 2 cut(s) 13, 410
NlaIII CATG 5 cut(s) 78, 195, 244, 531, 538
NlaIV GGNNCC 3 cut(s) 187, 231, 504
NmuCI GTSAC 1 cut(s) 205
NspI RCATGY 2 cut(s) 195, 244
PciSI GCTCTTC 1 cut(s) 430
PctI GAATGC 1 cut(s) 24
PflMI CCANNNNNTGG 1 cut(s) 601
PkrI GCNGC 1 cut(s) 568
Psp124BI GAGCTC 1 cut(s) 42
PspN4I GGNNCC 3 cut(s) 187, 231, 504
PspPI GGNCC 2 cut(s) 159, 531
PsuI RGATCY 2 cut(s) 13, 410
RsaI GTAC 1 cut(s) 7
RsaNI GTAC 1 cut(s) 6
SacI GAGCTC 1 cut(s) 42
SapI GCTCTTC 1 cut(s) 430
SaqAI TTAA 2 cut(s) 126, 548
SatI GCNGC 1 cut(s) 567
Sau3AI GATC 2 cut(s) 13, 410
Sau96I GGNCC 2 cut(s) 159, 531
ScrFI CCNGG 1 cut(s) 183
SduI GDGCHC 2 cut(s) 42, 425
SetI ASST 7 cut(s) 42, 106, 126, 292, 400, 494, 591
SfaNI GCATC 2 cut(s) 469, 693
SinI GGWCC 1 cut(s) 531
SmlI CTYRAG 1 cut(s) 497
SmoI CTYRAG 1 cut(s) 497
Sse9I AATT 6 cut(s) 44, 86, 106, 257, 359, 621
SsiI CCGC 3 cut(s) 233, 364, 567
SstI GAGCTC 1 cut(s) 42
StyD4I CCNGG 1 cut(s) 181
StyI CCWWGG 1 cut(s) 33
TaaI ACNGT 3 cut(s) 71, 152, 205
TaqII GACCGA 1 cut(s) 662
TasI AATT 6 cut(s) 44, 86, 106, 257, 359, 621
TauI GCSGC 1 cut(s) 569
Tru1I TTAA 2 cut(s) 126, 548
Tru9I TTAA 2 cut(s) 126, 548
TscAI CASTG 2 cut(s) 251, 643
TseFI GTSAC 1 cut(s) 205
Tsp45I GTSAC 1 cut(s) 205
TspDTI ATGAA 2 cut(s) 63, 691
TspRI CASTG 2 cut(s) 251, 643
Van91I CCANNNNNTGG 1 cut(s) 601
VpaK11BI GGWCC 1 cut(s) 531
XapI RAATTY 2 cut(s) 44, 257
XceI RCATGY 2 cut(s) 195, 244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.