pycom07g10820

Vesicle-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001420
Physical Location & Seq
Forward (+)
11035146 .. 11040524
5379 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g10820.2

Sequence Viewer

Length: 717 bp
ATGAGTACTGGAGATCTTCTTAGCATTCAGCCTTTAGAGCTCAAATTCCCATTTGAAGTGAAGAAACAGAGTTCATGTTCCTTGCAATTGACCAACAAAACAACTAATTATGTGGCCTTCAAGGTTAAAACAACCAATCCGAAAAAGTACTGCGTCCGGCCCAATGCCGGTGTTGTCTTACCCGGAACAACATGTGATGTTACAGTTACAATGCAAGCCCAAAAGGAGGCCCCGCCAGACATGCAGTGCAAGGACAAATTTCTCCTTCAAAGTGTTGTTGCACTAGAAGGTGCAACTACGAAAGACATAACTCCTGAAATGTTCAACAAGGAGGATGGTAAAATTGTGGAAGAGTTCAAATTGCGGGTAATCTACTTTCCTGCCAACCCCCCATCACCTGTCCCTGAAGGATCTGACGAGGGCTCCTCTCCAAAGGCTTCAGTGCTTGATAATGGGAATCAAAACACTTCCTTGTTTGATGCTGTATCAAGATCTCTTGAGGAACCAAAAGAGAAGTCGTCAGAGGCATCTCCTATGATTTCCAAGTTAACTGGAGAGAAGACTGCTGCTTTGCAACAAATTCAGAAGCTACGCCACGAACTGGATGTGTTGAGAAAAGAAAATAGCAAAAACAGCGGTGGCGGATTTTCATTATTGTTTGTGGTGCTGTTCGGTCTTTTAGGCATCTTGGTTGGCTACTTCATCAACAAAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

239

Amino Acids

26.05

Weight (kDa)

8.24

Isoelectric Point (pI)

46.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 601
AciI CCGC 4 cut(s) 233, 364, 636, 642
AclWI GGATC 1 cut(s) 418
AcsI RAATTY 3 cut(s) 44, 257, 579
AcuI CTGAAG 2 cut(s) 423, 426
AfaI GTAC 2 cut(s) 7, 149
AfiI CCNNNNNNNGG 3 cut(s) 167, 226, 601
AflIII ACRYGT 1 cut(s) 191
AgsI TTSAA 5 cut(s) 56, 121, 269, 325, 358
AluBI AGCT 2 cut(s) 40, 589
AluI AGCT 2 cut(s) 40, 589
Alw21I GWGCWC 1 cut(s) 42
AlwI GGATC 1 cut(s) 418
AoxI GGCC 3 cut(s) 114, 158, 228
ApeKI GCWGC 1 cut(s) 566
ApoI RAATTY 3 cut(s) 44, 257, 579
ArsI GACNNNNNNTTYG 4 cut(s) 500, 532, 553, 585
AspS9I GGNCC 2 cut(s) 159, 229
AsuC2I CCSGG 1 cut(s) 183
AsuHPI GGTGA 1 cut(s) 387
BanII GRGCYC 2 cut(s) 42, 425
BbsI GAAGAC 1 cut(s) 566
Bbv12I GWGCWC 1 cut(s) 42
BbvI GCAGC 1 cut(s) 553
BccI CCATC 2 cut(s) 329, 400
BcnI CCSGG 1 cut(s) 183
BfaI CTAG 1 cut(s) 284
BglII AGATCT 2 cut(s) 13, 491
BisI GCNGC 1 cut(s) 567
BlsI GCNGC 1 cut(s) 568
BmcAI AGTACT 2 cut(s) 7, 149
Bme1390I CCNGG 1 cut(s) 183
BmgT120I GGNCC 2 cut(s) 159, 229
BmiI GGNNCC 3 cut(s) 231, 424, 504
BmrFI CCNGG 1 cut(s) 183
BmsI GCATC 3 cut(s) 469, 536, 693
BpiI GAAGAC 1 cut(s) 566
BplI GAGNNNNNCTC 2 cut(s) 410, 442
BpmI CTGGAG 2 cut(s) 30, 573
BpuEI CTTGAG 1 cut(s) 518
BpuMI CCSGG 1 cut(s) 183
BsaXI ACNNNNNCTCC 2 cut(s) 407, 437
Bsc4I CCNNNNNNNGG 3 cut(s) 167, 226, 601
Bse118I RCCGGY 1 cut(s) 167
Bse1I ACTGG 3 cut(s) 13, 556, 606
BseGI GGATG 2 cut(s) 340, 610
BseLI CCNNNNNNNGG 3 cut(s) 167, 226, 601
BseNI ACTGG 3 cut(s) 13, 556, 606
BseRI GAGGAG 1 cut(s) 415
BseXI GCAGC 1 cut(s) 553
BshFI GGCC 3 cut(s) 116, 160, 230
BsiHKAI GWGCWC 1 cut(s) 42
BsiSI CCGG 3 cut(s) 157, 168, 183
BslFI GGGAC 1 cut(s) 386
BslI CCNNNNNNNGG 3 cut(s) 167, 226, 601
BsmFI GGGAC 1 cut(s) 386
BsmI GAATGC 1 cut(s) 24
BsnI GGCC 3 cut(s) 116, 160, 230
Bsp1286I GDGCHC 2 cut(s) 42, 425
Bsp143I GATC 3 cut(s) 13, 410, 491
BspACI CCGC 4 cut(s) 233, 364, 636, 642
BspANI GGCC 3 cut(s) 116, 160, 230
BspLI GGNNCC 3 cut(s) 231, 424, 504
BspPI GGATC 1 cut(s) 418
BsrFI RCCGGY 1 cut(s) 167
BsrI ACTGG 3 cut(s) 13, 556, 606
BssAI RCCGGY 1 cut(s) 167
BssMI GATC 3 cut(s) 13, 410, 491
Bst4CI ACNGT 1 cut(s) 205
Bst6I CTCTTC 1 cut(s) 345
BstC8I GCNNGC 1 cut(s) 216
BstDEI CTNAG 1 cut(s) 20
BstF5I GGATG 2 cut(s) 340, 610
BstKTI GATC 3 cut(s) 16, 413, 494
BstMBI GATC 3 cut(s) 13, 410, 491
BstMWI GCNNNNNNNGC 3 cut(s) 37, 241, 633
BstNSI RCATGY 2 cut(s) 195, 244
BstSCI CCNGG 1 cut(s) 181
BstV1I GCAGC 1 cut(s) 553
BstV2I GAAGAC 1 cut(s) 566
BstX2I RGATCY 3 cut(s) 13, 410, 491
BstYI RGATCY 3 cut(s) 13, 410, 491
BsuRI GGCC 3 cut(s) 116, 160, 230
BtsCI GGATG 2 cut(s) 340, 610
BtsI GCAGTG 1 cut(s) 251
BtsIMutI CAGTG 2 cut(s) 251, 447
Cac8I GCNNGC 1 cut(s) 216
Cfr10I RCCGGY 1 cut(s) 167
Cfr13I GGNCC 2 cut(s) 159, 229
CseI GACGC 1 cut(s) 142
Csp6I GTAC 2 cut(s) 6, 148
CviAII CATG 3 cut(s) 75, 192, 241
CviQI GTAC 2 cut(s) 6, 148
DdeI CTNAG 1 cut(s) 20
DpnI GATC 3 cut(s) 15, 412, 493
DpnII GATC 3 cut(s) 13, 410, 491
Eam1104I CTCTTC 1 cut(s) 345
EarI CTCTTC 1 cut(s) 345
EciI GGCGGA 1 cut(s) 657
Ecl136II GAGCTC 1 cut(s) 40
Eco24I GRGCYC 2 cut(s) 42, 425
Eco53kI GAGCTC 1 cut(s) 40
Eco57I CTGAAG 2 cut(s) 423, 426
EcoICRI GAGCTC 1 cut(s) 40
EcoO109I RGGNCCY 1 cut(s) 229
EcoT38I GRGCYC 2 cut(s) 42, 425
FaeI CATG 3 cut(s) 78, 195, 244
FaiI YATR 7 cut(s) 76, 111, 193, 242, 308, 536, 715
FaqI GGGAC 1 cut(s) 386
FatI CATG 3 cut(s) 74, 191, 240
FauI CCCGC 2 cut(s) 240, 357
Fnu4HI GCNGC 1 cut(s) 567
FokI GGATG 2 cut(s) 347, 617
FriOI GRGCYC 2 cut(s) 42, 425
Fsp4HI GCNGC 1 cut(s) 567
FspBI CTAG 1 cut(s) 284
GluI GCNGC 1 cut(s) 567
GsuI CTGGAG 2 cut(s) 30, 573
HaeIII GGCC 3 cut(s) 116, 160, 230
HapII CCGG 3 cut(s) 157, 168, 183
HgaI GACGC 1 cut(s) 142
Hin1II CATG 3 cut(s) 78, 195, 244
HincII GTYRAC 1 cut(s) 549
HindII GTYRAC 1 cut(s) 549
HinfI GANTC 1 cut(s) 457
HpaI GTTAAC 1 cut(s) 549
HpaII CCGG 3 cut(s) 157, 168, 183
HphI GGTGA 1 cut(s) 387
Hpy166II GTNNAC 1 cut(s) 549
Hpy188I TCNGA 4 cut(s) 141, 415, 523, 585
Hpy188III TCNNGA 3 cut(s) 314, 489, 497
Hpy8I GTNNAC 1 cut(s) 549
HpyAV CCTTC 4 cut(s) 127, 275, 281, 401
HpyCH4III ACNGT 1 cut(s) 205
HpyCH4V TGCA 7 cut(s) 85, 214, 244, 249, 281, 293, 574
HpyF10VI GCNNNNNNNGC 3 cut(s) 37, 241, 633
HpyF3I CTNAG 1 cut(s) 20
Hsp92II CATG 3 cut(s) 78, 195, 244
KspAI GTTAAC 1 cut(s) 549
Kzo9I GATC 3 cut(s) 13, 410, 491
LmnI GCTCC 1 cut(s) 428
Lsp1109I GCAGC 1 cut(s) 553
LweI GCATC 3 cut(s) 469, 536, 693
MaeI CTAG 1 cut(s) 284
MaeIII GTNAC 2 cut(s) 199, 205
MalI GATC 3 cut(s) 15, 412, 493
MboI GATC 3 cut(s) 13, 410, 491
MboII GAAGA 4 cut(s) 8, 73, 362, 571
MfeI CAATTG 1 cut(s) 86
MflI RGATCY 3 cut(s) 13, 410, 491
MhlI GDGCHC 2 cut(s) 42, 425
MluCI AATT 7 cut(s) 44, 86, 106, 257, 342, 359, 579
MnlI CCTC 6 cut(s) 220, 325, 412, 436, 493, 517
MseI TTAA 2 cut(s) 126, 548
MspA1I CMGCKG 1 cut(s) 636
MspI CCGG 3 cut(s) 157, 168, 183
MspR9I CCNGG 1 cut(s) 183
MunI CAATTG 1 cut(s) 86
Mva1269I GAATGC 1 cut(s) 24
MwoI GCNNNNNNNGC 3 cut(s) 37, 241, 633
NciI CCSGG 1 cut(s) 183
NdeII GATC 3 cut(s) 13, 410, 491
NlaIII CATG 3 cut(s) 78, 195, 244
NlaIV GGNNCC 3 cut(s) 231, 424, 504
NspI RCATGY 2 cut(s) 195, 244
PciI ACATGT 1 cut(s) 191
PctI GAATGC 1 cut(s) 24
PfeI GAWTC 1 cut(s) 457
PflMI CCANNNNNTGG 1 cut(s) 601
PkrI GCNGC 1 cut(s) 568
PscI ACATGT 1 cut(s) 191
Psp124BI GAGCTC 1 cut(s) 42
PspN4I GGNNCC 3 cut(s) 231, 424, 504
PspPI GGNCC 2 cut(s) 159, 229
PsuI RGATCY 3 cut(s) 13, 410, 491
RsaI GTAC 2 cut(s) 7, 149
RsaNI GTAC 2 cut(s) 6, 148
SacI GAGCTC 1 cut(s) 42
SaqAI TTAA 2 cut(s) 126, 548
SatI GCNGC 1 cut(s) 567
Sau3AI GATC 3 cut(s) 13, 410, 491
Sau96I GGNCC 2 cut(s) 159, 229
ScaI AGTACT 2 cut(s) 7, 149
ScrFI CCNGG 1 cut(s) 183
SduI GDGCHC 2 cut(s) 42, 425
SetI ASST 5 cut(s) 42, 126, 292, 400, 591
SfaNI GCATC 3 cut(s) 469, 536, 693
SmlI CTYRAG 1 cut(s) 497
SmoI CTYRAG 1 cut(s) 497
Sse9I AATT 7 cut(s) 44, 86, 106, 257, 342, 359, 579
SsiI CCGC 4 cut(s) 233, 364, 636, 642
SspMI CTAG 1 cut(s) 284
SstI GAGCTC 1 cut(s) 42
StyD4I CCNGG 1 cut(s) 181
TaaI ACNGT 1 cut(s) 205
TaqII GACCGA 1 cut(s) 662
TasI AATT 7 cut(s) 44, 86, 106, 257, 342, 359, 579
TatI WGTACW 2 cut(s) 5, 147
TfiI GAWTC 1 cut(s) 457
Tru1I TTAA 2 cut(s) 126, 548
Tru9I TTAA 2 cut(s) 126, 548
TscAI CASTG 2 cut(s) 251, 447
TseI GCWGC 1 cut(s) 566
TspDTI ATGAA 3 cut(s) 63, 639, 691
TspRI CASTG 2 cut(s) 251, 447
Van91I CCANNNNNTGG 1 cut(s) 601
XapI RAATTY 3 cut(s) 44, 257, 579
XceI RCATGY 2 cut(s) 195, 244
XspI CTAG 1 cut(s) 284
ZrmI AGTACT 2 cut(s) 7, 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.