Rh1BG186200

Vesicle-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0001420
Physical Location & Seq
Reverse (-)
30085566 .. 30089511
3946 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG186200.1

Sequence Viewer

Length: 558 bp
ATGAGCACCGGAGATCTCCTCAGCATTCAGCCCACGGAGCTCAAATTCCCATTTGAACTGAAGAAGCAGAGCTCGTGTTCTTTGCAATTGACCAACAAAACAGATAAATATGTGGCATTTAAGGTTAAGACAACCAATCCAAAGAAGTATTGTGTCAGACCCAATGCTGGTGTTGTTTTGCCCGGATCGACATGTGATGTTACAGTTACGATGCAAGCCCAAAAGGAGGCACCCCCAGATATGCAGTGTAAGGACAAATTTCTCCTTCAAAGTGTTTTTGCGCCAGATGGTTCAACTACAAAAGACATAACTCCTGAAATGTTCAACAAGGATGATGGTAAAGTTGTGGAAGAGTTCAAATTGCGGGTAATTTACTTTCCTGCCAACCCCCCGTCACCTGTCCCTGAAGGATCTGATGAGGGCTCTTCTCCCAGGGCAGTGGTTGAGAATGGGACTCAAAATCCTTCGTTGTTTGATGCTGTGTCAAGATCTCTTGAGCAACCAAAAGAGAAATCTTCAGAGGTGAGATCCCTTAGTAATCTTTTTGACAGAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

185

Amino Acids

20.41

Weight (kDa)

6.37

Isoelectric Point (pI)

52.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 7 - 110 4.4e-35 MSP (Major sperm protein) domain
Cep192_D6 PF22076 7 - 78 1.3e-07 Cep192 domain 6
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 229
AciI CCGC 1 cut(s) 364
AclWI GGATC 3 cut(s) 193, 418, 522
AcsI RAATTY 2 cut(s) 44, 257
AcuI CTGAAG 3 cut(s) 80, 426, 501
AfiI CCNNNNNNNGG 2 cut(s) 167, 226
AflIII ACRYGT 1 cut(s) 191
AgsI TTSAA 5 cut(s) 56, 269, 294, 325, 358
AjnI CCWGG 1 cut(s) 431
AluBI AGCT 2 cut(s) 40, 72
AluI AGCT 2 cut(s) 40, 72
Alw21I GWGCWC 3 cut(s) 8, 42, 74
AlwI GGATC 3 cut(s) 193, 418, 522
ApoI RAATTY 2 cut(s) 44, 257
AspLEI GCGC 1 cut(s) 283
AsuC2I CCSGG 1 cut(s) 183
AsuHPI GGTGA 2 cut(s) 387, 535
BanI GGYRCC 1 cut(s) 229
BanII GRGCYC 3 cut(s) 42, 74, 425
BauI CACGAG 1 cut(s) 73
Bbv12I GWGCWC 3 cut(s) 8, 42, 74
BbvCI CCTCAGC 1 cut(s) 20
BccI CCATC 2 cut(s) 281, 329
BciT130I CCWGG 1 cut(s) 433
BcnI CCSGG 1 cut(s) 183
BglII AGATCT 2 cut(s) 13, 488
Bme1390I CCNGG 2 cut(s) 183, 433
BmiI GGNNCC 1 cut(s) 231
BmrFI CCNGG 2 cut(s) 183, 433
BmsI GCATC 2 cut(s) 201, 466
BplI GAGNNNNNCTC 1 cut(s) 35
Bpu10I CCTNAGC 1 cut(s) 20
BpuEI CTTGAG 1 cut(s) 515
BpuMI CCSGG 1 cut(s) 183
BsaJI CCNNGG 3 cut(s) 33, 431, 432
BsaWI WCCGGW 1 cut(s) 8
Bsc4I CCNNNNNNNGG 2 cut(s) 167, 226
BseBI CCWGG 1 cut(s) 433
BseDI CCNNGG 3 cut(s) 33, 431, 432
BseGI GGATG 1 cut(s) 337
BseLI CCNNNNNNNGG 2 cut(s) 167, 226
BseMII CTCAG 1 cut(s) 34
BseRI GAGGAG 1 cut(s) 8
BshNI GGYRCC 1 cut(s) 229
BsiHKAI GWGCWC 3 cut(s) 8, 42, 74
BsiSI CCGG 2 cut(s) 9, 183
BslFI GGGAC 2 cut(s) 386, 466
BslI CCNNNNNNNGG 2 cut(s) 167, 226
BsmFI GGGAC 2 cut(s) 386, 466
BsmI GAATGC 1 cut(s) 24
Bsp1286I GDGCHC 4 cut(s) 8, 42, 74, 425
Bsp143I GATC 5 cut(s) 13, 185, 410, 488, 527
BspACI CCGC 1 cut(s) 364
BspCNI CTCAG 1 cut(s) 33
BspLI GGNNCC 1 cut(s) 231
BspPI GGATC 3 cut(s) 193, 418, 522
BspQI GCTCTTC 1 cut(s) 430
BspT107I GGYRCC 1 cut(s) 229
BssECI CCNNGG 3 cut(s) 33, 431, 432
BssMI GATC 5 cut(s) 13, 185, 410, 488, 527
BssSI CACGAG 1 cut(s) 73
Bst2BI CACGAG 1 cut(s) 73
Bst2UI CCWGG 1 cut(s) 433
Bst4CI ACNGT 1 cut(s) 205
Bst6I CTCTTC 2 cut(s) 345, 430
BstC8I GCNNGC 1 cut(s) 216
BstDEI CTNAG 2 cut(s) 20, 533
BstDSI CCRYGG 1 cut(s) 33
BstF5I GGATG 1 cut(s) 337
BstHHI GCGC 1 cut(s) 283
BstKTI GATC 5 cut(s) 16, 188, 413, 491, 530
BstMBI GATC 5 cut(s) 13, 185, 410, 488, 527
BstMWI GCNNNNNNNGC 1 cut(s) 37
BstNI CCWGG 1 cut(s) 433
BstNSI RCATGY 1 cut(s) 195
BstSCI CCNGG 2 cut(s) 181, 431
BstX2I RGATCY 4 cut(s) 13, 410, 488, 527
BstXI CCANNNNNNTGG 1 cut(s) 439
BstYI RGATCY 4 cut(s) 13, 410, 488, 527
BtgI CCRYGG 1 cut(s) 33
BtsCI GGATG 1 cut(s) 337
BtsI GCAGTG 2 cut(s) 251, 444
BtsIMutI CAGTG 2 cut(s) 251, 444
Cac8I GCNNGC 1 cut(s) 216
CfoI GCGC 1 cut(s) 283
CviAII CATG 1 cut(s) 192
CviJI RGCY 5 cut(s) 31, 40, 72, 218, 423
CviKI_1 RGCY 5 cut(s) 31, 40, 72, 218, 423
DdeI CTNAG 2 cut(s) 20, 533
DpnI GATC 5 cut(s) 15, 187, 412, 490, 529
DpnII GATC 5 cut(s) 13, 185, 410, 488, 527
Eam1104I CTCTTC 2 cut(s) 345, 430
EarI CTCTTC 2 cut(s) 345, 430
Ecl136II GAGCTC 2 cut(s) 40, 72
Eco24I GRGCYC 3 cut(s) 42, 74, 425
Eco53kI GAGCTC 2 cut(s) 40, 72
Eco57I CTGAAG 3 cut(s) 80, 426, 501
EcoICRI GAGCTC 2 cut(s) 40, 72
EcoRII CCWGG 1 cut(s) 431
EcoT38I GRGCYC 3 cut(s) 42, 74, 425
FaeI CATG 1 cut(s) 195
FaiI YATR 4 cut(s) 111, 193, 242, 308
FaqI GGGAC 2 cut(s) 386, 466
FatI CATG 1 cut(s) 191
FauI CCCGC 1 cut(s) 357
FokI GGATG 1 cut(s) 344
FriOI GRGCYC 3 cut(s) 42, 74, 425
GlaI GCGC 1 cut(s) 282
HapII CCGG 2 cut(s) 9, 183
HhaI GCGC 1 cut(s) 283
Hin1II CATG 1 cut(s) 195
Hin6I GCGC 1 cut(s) 281
HinP1I GCGC 1 cut(s) 281
HinfI GANTC 1 cut(s) 454
HpaII CCGG 2 cut(s) 9, 183
HphI GGTGA 2 cut(s) 387, 535
Hpy188I TCNGA 3 cut(s) 158, 415, 520
Hpy188III TCNNGA 3 cut(s) 314, 486, 494
HpyAV CCTTC 3 cut(s) 275, 401, 474
HpyCH4III ACNGT 1 cut(s) 205
HpyCH4V TGCA 3 cut(s) 85, 214, 244
HpyF10VI GCNNNNNNNGC 1 cut(s) 37
HpyF3I CTNAG 2 cut(s) 20, 533
Hsp92II CATG 1 cut(s) 195
HspAI GCGC 1 cut(s) 281
Kzo9I GATC 5 cut(s) 13, 185, 410, 488, 527
LguI GCTCTTC 1 cut(s) 430
LmnI GCTCC 1 cut(s) 37
LweI GCATC 2 cut(s) 201, 466
MaeIII GTNAC 3 cut(s) 199, 205, 393
MalI GATC 5 cut(s) 15, 187, 412, 490, 529
MboI GATC 5 cut(s) 13, 185, 410, 488, 527
MboII GAAGA 4 cut(s) 73, 362, 417, 507
MfeI CAATTG 1 cut(s) 86
MflI RGATCY 4 cut(s) 13, 410, 488, 527
MhlI GDGCHC 4 cut(s) 8, 42, 74, 425
MluCI AATT 5 cut(s) 44, 86, 257, 359, 369
MlyI GAGTC 1 cut(s) 448
MnlI CCTC 4 cut(s) 29, 220, 412, 514
MseI TTAA 2 cut(s) 120, 126
MspI CCGG 2 cut(s) 9, 183
MspR9I CCNGG 2 cut(s) 183, 433
MunI CAATTG 1 cut(s) 86
Mva1269I GAATGC 1 cut(s) 24
MvaI CCWGG 1 cut(s) 433
MwoI GCNNNNNNNGC 1 cut(s) 37
NciI CCSGG 1 cut(s) 183
NdeII GATC 5 cut(s) 13, 185, 410, 488, 527
NlaIII CATG 1 cut(s) 195
NlaIV GGNNCC 1 cut(s) 231
NmuCI GTSAC 1 cut(s) 393
NspI RCATGY 1 cut(s) 195
PasI CCCWGGG 1 cut(s) 432
PciI ACATGT 1 cut(s) 191
PciSI GCTCTTC 1 cut(s) 430
PctI GAATGC 1 cut(s) 24
PleI GAGTC 1 cut(s) 448
PpsI GAGTC 1 cut(s) 448
PscI ACATGT 1 cut(s) 191
Psp124BI GAGCTC 2 cut(s) 42, 74
Psp6I CCWGG 1 cut(s) 431
PspGI CCWGG 1 cut(s) 431
PspN4I GGNNCC 1 cut(s) 231
PsuI RGATCY 4 cut(s) 13, 410, 488, 527
SacI GAGCTC 2 cut(s) 42, 74
SapI GCTCTTC 1 cut(s) 430
SaqAI TTAA 2 cut(s) 120, 126
Sau3AI GATC 5 cut(s) 13, 185, 410, 488, 527
SchI GAGTC 1 cut(s) 448
ScrFI CCNGG 2 cut(s) 183, 433
SduI GDGCHC 4 cut(s) 8, 42, 74, 425
SetI ASST 5 cut(s) 42, 74, 126, 400, 525
SfaNI GCATC 2 cut(s) 201, 466
SmlI CTYRAG 1 cut(s) 494
SmoI CTYRAG 1 cut(s) 494
Sse9I AATT 5 cut(s) 44, 86, 257, 359, 369
SsiI CCGC 1 cut(s) 364
SstI GAGCTC 2 cut(s) 42, 74
StyD4I CCNGG 2 cut(s) 181, 431
TaaI ACNGT 1 cut(s) 205
TaqI TCGA 1 cut(s) 188
TasI AATT 5 cut(s) 44, 86, 257, 359, 369
Tru1I TTAA 2 cut(s) 120, 126
Tru9I TTAA 2 cut(s) 120, 126
TscAI CASTG 2 cut(s) 251, 444
TseFI GTSAC 1 cut(s) 393
Tsp45I GTSAC 1 cut(s) 393
TspGWI ACGGA 1 cut(s) 50
TspRI CASTG 2 cut(s) 251, 444
XapI RAATTY 2 cut(s) 44, 257
XceI RCATGY 1 cut(s) 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.