Rroxscaffold_1G00035870

Vesicle-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001420
Physical Location & Seq
Reverse (-)
52230299 .. 52230893
595 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00035870.1

Sequence Viewer

Length: 498 bp
ATGAGCACCGGAGATCTCCTCAGCATTCAGCCCACGAAGCTCAAATTCCCATTTGAACTGAAGAAGCAGAGCTCGTGTTCTTTGCAATTGACCAACAAAACAGATAAATATGTGGCATTTAAGGTTAAGACAACCAATCCAAAGAAGTATTGTGTCAGACCCAATGCTGGTGTTGTTGTCCCCGGATCGACATGTGATGTTACAGTTACGATGCAAGCCCAAAAAGGAGGCACCCCCAGATATGCAGTTTGTGGAAGAGTTCAAATTGCGGGTAATTTACTTTCCTGCCAACCCCCGTCACCTGTCCCTGAAGGATCTGATGAGGGCTCTTCTCCCAGGGCAGTGGTTGAGAATGGGACCCAAAATCCTTCCTTGTTTGATGCTGTGTCAAGATCTCTTGAGCAACCAAAAGAGAAATCTTCAGAGCGGATCATTGCTTTCATGAGCAAACATATCAGCCTCGTTGGGGTTACTGGGGAAGGTACATGCAGCCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

165

Amino Acids

17.62

Weight (kDa)

9.2

Isoelectric Point (pI)

47.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 7 - 77 1.8e-26 MSP (Major sperm protein) domain
Cep192_D6 PF22076 7 - 74 1.6e-07 Cep192 domain 6
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 230
AccBSI CCGCTC 1 cut(s) 427
AciI CCGC 2 cut(s) 269, 427
AclWI GGATC 3 cut(s) 193, 322, 437
AcsI RAATTY 1 cut(s) 44
AcuI CTGAAG 3 cut(s) 80, 330, 405
AfaI GTAC 1 cut(s) 484
AfiI CCNNNNNNNGG 2 cut(s) 167, 466
AflIII ACRYGT 1 cut(s) 191
AgsI TTSAA 2 cut(s) 56, 263
AjnI CCWGG 1 cut(s) 335
AluBI AGCT 2 cut(s) 40, 72
AluI AGCT 2 cut(s) 40, 72
Alw21I GWGCWC 2 cut(s) 8, 74
AlwI GGATC 3 cut(s) 193, 322, 437
ApeKI GCWGC 1 cut(s) 489
ApoI RAATTY 1 cut(s) 44
AspS9I GGNCC 1 cut(s) 357
AsuC2I CCSGG 1 cut(s) 183
AsuHPI GGTGA 1 cut(s) 291
AvaII GGWCC 1 cut(s) 357
BanI GGYRCC 1 cut(s) 230
BanII GRGCYC 2 cut(s) 74, 329
BauI CACGAG 1 cut(s) 73
Bbv12I GWGCWC 2 cut(s) 8, 74
BbvCI CCTCAGC 1 cut(s) 20
BciT130I CCWGG 1 cut(s) 337
BcnI CCSGG 1 cut(s) 183
BfaI CTAG 1 cut(s) 496
BglII AGATCT 2 cut(s) 13, 392
BisI GCNGC 1 cut(s) 490
BlsI GCNGC 1 cut(s) 491
Bme1390I CCNGG 2 cut(s) 183, 337
Bme18I GGWCC 1 cut(s) 357
BmgT120I GGNCC 1 cut(s) 357
BmiI GGNNCC 3 cut(s) 232, 358, 359
BmrFI CCNGG 2 cut(s) 183, 337
BmrI ACTGGG 1 cut(s) 483
BmsI GCATC 2 cut(s) 201, 370
BmuI ACTGGG 1 cut(s) 483
BplI GAGNNNNNCTC 1 cut(s) 35
Bpu10I CCTNAGC 1 cut(s) 20
BpuEI CTTGAG 1 cut(s) 419
BpuMI CCSGG 1 cut(s) 183
BsaJI CCNNGG 3 cut(s) 181, 335, 336
BsaWI WCCGGW 1 cut(s) 8
Bsc4I CCNNNNNNNGG 2 cut(s) 167, 466
Bse1I ACTGG 1 cut(s) 478
Bse3DI GCAATG 1 cut(s) 432
BseBI CCWGG 1 cut(s) 337
BseDI CCNNGG 3 cut(s) 181, 335, 336
BseLI CCNNNNNNNGG 2 cut(s) 167, 466
BseMI GCAATG 1 cut(s) 432
BseMII CTCAG 1 cut(s) 34
BseNI ACTGG 1 cut(s) 478
BseRI GAGGAG 1 cut(s) 8
BshNI GGYRCC 1 cut(s) 230
BsiHKAI GWGCWC 2 cut(s) 8, 74
BsiSI CCGG 2 cut(s) 9, 183
BslFI GGGAC 3 cut(s) 164, 290, 370
BslI CCNNNNNNNGG 2 cut(s) 167, 466
BsmFI GGGAC 3 cut(s) 164, 290, 370
BsmI GAATGC 1 cut(s) 24
Bsp1286I GDGCHC 3 cut(s) 8, 74, 329
Bsp143I GATC 5 cut(s) 13, 185, 314, 392, 429
BspACI CCGC 2 cut(s) 269, 427
BspCNI CTCAG 1 cut(s) 33
BspHI TCATGA 1 cut(s) 441
BspLI GGNNCC 3 cut(s) 232, 358, 359
BspPI GGATC 3 cut(s) 193, 322, 437
BspQI GCTCTTC 1 cut(s) 334
BspT107I GGYRCC 1 cut(s) 230
BsrBI CCGCTC 1 cut(s) 427
BsrDI GCAATG 1 cut(s) 432
BsrI ACTGG 1 cut(s) 478
BssECI CCNNGG 3 cut(s) 181, 335, 336
BssMI GATC 5 cut(s) 13, 185, 314, 392, 429
BssSI CACGAG 1 cut(s) 73
Bst2BI CACGAG 1 cut(s) 73
Bst2UI CCWGG 1 cut(s) 337
Bst4CI ACNGT 1 cut(s) 205
Bst6I CTCTTC 2 cut(s) 250, 334
BstC8I GCNNGC 1 cut(s) 216
BstDEI CTNAG 1 cut(s) 20
BstKTI GATC 5 cut(s) 16, 188, 317, 395, 432
BstMBI GATC 5 cut(s) 13, 185, 314, 392, 429
BstMWI GCNNNNNNNGC 1 cut(s) 37
BstNI CCWGG 1 cut(s) 337
BstNSI RCATGY 2 cut(s) 195, 489
BstSCI CCNGG 2 cut(s) 181, 335
BstX2I RGATCY 3 cut(s) 13, 314, 392
BstXI CCANNNNNNTGG 1 cut(s) 343
BstYI RGATCY 3 cut(s) 13, 314, 392
BtsI GCAGTG 1 cut(s) 348
BtsIMutI CAGTG 1 cut(s) 348
Cac8I GCNNGC 1 cut(s) 216
CciI TCATGA 1 cut(s) 441
Cfr13I GGNCC 1 cut(s) 357
Csp6I GTAC 1 cut(s) 483
CviAII CATG 3 cut(s) 192, 442, 486
CviJI RGCY 7 cut(s) 31, 40, 72, 218, 327, 459, 492
CviKI_1 RGCY 7 cut(s) 31, 40, 72, 218, 327, 459, 492
CviQI GTAC 1 cut(s) 483
DdeI CTNAG 1 cut(s) 20
DpnI GATC 5 cut(s) 15, 187, 316, 394, 431
DpnII GATC 5 cut(s) 13, 185, 314, 392, 429
Eam1104I CTCTTC 2 cut(s) 250, 334
EarI CTCTTC 2 cut(s) 250, 334
Ecl136II GAGCTC 1 cut(s) 72
Eco24I GRGCYC 2 cut(s) 74, 329
Eco47I GGWCC 1 cut(s) 357
Eco53kI GAGCTC 1 cut(s) 72
Eco57I CTGAAG 3 cut(s) 80, 330, 405
EcoICRI GAGCTC 1 cut(s) 72
EcoO109I RGGNCCY 1 cut(s) 357
EcoRII CCWGG 1 cut(s) 335
EcoT38I GRGCYC 2 cut(s) 74, 329
FaeI CATG 3 cut(s) 195, 445, 489
FaiI YATR 6 cut(s) 111, 193, 243, 443, 453, 487
FaqI GGGAC 3 cut(s) 164, 290, 370
FatI CATG 3 cut(s) 191, 441, 485
FauI CCCGC 1 cut(s) 262
Fnu4HI GCNGC 1 cut(s) 490
FriOI GRGCYC 2 cut(s) 74, 329
Fsp4HI GCNGC 1 cut(s) 490
FspBI CTAG 1 cut(s) 496
GluI GCNGC 1 cut(s) 490
HapII CCGG 2 cut(s) 9, 183
Hin1II CATG 3 cut(s) 195, 445, 489
HpaII CCGG 2 cut(s) 9, 183
HphI GGTGA 1 cut(s) 291
Hpy188I TCNGA 3 cut(s) 158, 319, 424
Hpy188III TCNNGA 3 cut(s) 390, 398, 442
HpyAV CCTTC 3 cut(s) 305, 378, 473
HpyCH4III ACNGT 1 cut(s) 205
HpyCH4V TGCA 4 cut(s) 85, 214, 245, 489
HpyF10VI GCNNNNNNNGC 1 cut(s) 37
HpyF3I CTNAG 1 cut(s) 20
Hsp92II CATG 3 cut(s) 195, 445, 489
KflI GGGWCCC 1 cut(s) 357
Kzo9I GATC 5 cut(s) 13, 185, 314, 392, 429
LguI GCTCTTC 1 cut(s) 334
LweI GCATC 2 cut(s) 201, 370
MaeI CTAG 1 cut(s) 496
MaeIII GTNAC 4 cut(s) 199, 205, 297, 469
MalI GATC 5 cut(s) 15, 187, 316, 394, 431
MbiI CCGCTC 1 cut(s) 427
MboI GATC 5 cut(s) 13, 185, 314, 392, 429
MboII GAAGA 4 cut(s) 73, 267, 321, 411
MfeI CAATTG 1 cut(s) 86
MflI RGATCY 3 cut(s) 13, 314, 392
MhlI GDGCHC 3 cut(s) 8, 74, 329
MluCI AATT 4 cut(s) 44, 86, 264, 274
MnlI CCTC 4 cut(s) 29, 221, 316, 470
MseI TTAA 2 cut(s) 120, 126
MspI CCGG 2 cut(s) 9, 183
MspR9I CCNGG 2 cut(s) 183, 337
MunI CAATTG 1 cut(s) 86
Mva1269I GAATGC 1 cut(s) 24
MvaI CCWGG 1 cut(s) 337
MwoI GCNNNNNNNGC 1 cut(s) 37
NciI CCSGG 1 cut(s) 183
NdeII GATC 5 cut(s) 13, 185, 314, 392, 429
NlaIII CATG 3 cut(s) 195, 445, 489
NlaIV GGNNCC 3 cut(s) 232, 358, 359
NmuCI GTSAC 1 cut(s) 297
NspI RCATGY 2 cut(s) 195, 489
PagI TCATGA 1 cut(s) 441
PasI CCCWGGG 1 cut(s) 336
PciI ACATGT 1 cut(s) 191
PciSI GCTCTTC 1 cut(s) 334
PctI GAATGC 1 cut(s) 24
PkrI GCNGC 1 cut(s) 491
PpuMI RGGWCCY 1 cut(s) 357
PscI ACATGT 1 cut(s) 191
Psp124BI GAGCTC 1 cut(s) 74
Psp5II RGGWCCY 1 cut(s) 357
Psp6I CCWGG 1 cut(s) 335
PspGI CCWGG 1 cut(s) 335
PspN4I GGNNCC 3 cut(s) 232, 358, 359
PspPI GGNCC 1 cut(s) 357
PspPPI RGGWCCY 1 cut(s) 357
PsuI RGATCY 3 cut(s) 13, 314, 392
RsaI GTAC 1 cut(s) 484
RsaNI GTAC 1 cut(s) 483
SacI GAGCTC 1 cut(s) 74
SapI GCTCTTC 1 cut(s) 334
SaqAI TTAA 2 cut(s) 120, 126
SatI GCNGC 1 cut(s) 490
Sau3AI GATC 5 cut(s) 13, 185, 314, 392, 429
Sau96I GGNCC 1 cut(s) 357
ScrFI CCNGG 2 cut(s) 183, 337
SduI GDGCHC 3 cut(s) 8, 74, 329
SetI ASST 5 cut(s) 42, 74, 126, 304, 484
SfaNI GCATC 2 cut(s) 201, 370
SinI GGWCC 1 cut(s) 357
SmlI CTYRAG 1 cut(s) 398
SmoI CTYRAG 1 cut(s) 398
Sse9I AATT 4 cut(s) 44, 86, 264, 274
SsiI CCGC 2 cut(s) 269, 427
SspMI CTAG 1 cut(s) 496
SstI GAGCTC 1 cut(s) 74
StyD4I CCNGG 2 cut(s) 181, 335
TaaI ACNGT 1 cut(s) 205
TaqI TCGA 1 cut(s) 188
TasI AATT 4 cut(s) 44, 86, 264, 274
Tru1I TTAA 2 cut(s) 120, 126
Tru9I TTAA 2 cut(s) 120, 126
TscAI CASTG 1 cut(s) 348
TseFI GTSAC 1 cut(s) 297
TseI GCWGC 1 cut(s) 489
Tsp45I GTSAC 1 cut(s) 297
TspDTI ATGAA 1 cut(s) 430
TspRI CASTG 1 cut(s) 348
VpaK11BI GGWCC 1 cut(s) 357
XapI RAATTY 1 cut(s) 44
XceI RCATGY 2 cut(s) 195, 489
XspI CTAG 1 cut(s) 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.