Rorug01G0202700

Vesicle-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001420
Physical Location & Seq
Forward (+)
28196311 .. 28197552
1242 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0202700.1

Sequence Viewer

Length: 1047 bp
ATGGAGACATCTATAATGCTAAGGTGTGCCAGTTTTATGTTGTTTATGGTCTGGACGCTGTGTATATCATCGTCTCTGGCATGTACTGAAAAGCAGAAGCCGATAGGGTATGAGCAAAAATCCATGAAGGAGAGGTACGAAAGGTGGCTGACAAAATACGACCGAAGATACAAGAATAGAGAGGAGTGGGAATATCGTTTTGGAATTTACCAGTCCAACGTTGAGCTTGTCGATTTCATAAACTCTCAAAACCTGTCATACAAACTCACTGACAACAAATTTGCAGACATGACAAACTTAGAGTTTACAAAAACCCACTTGGGTTTACAAGCTGGTAGAAATCCAAAGACAAAGTTCAGGTATGAGTGGAAGAAGGACTTGCCAACTACAGTGGATTGGAGAAAGAATGGGAGCGTAACTCCGGTCAGGGACCAAGGCCGATGTGGTAGCTGTTGGGCATTCTCTGCAGTTGCCGCTGTGGAAGGCCTTCACGAAATCAATACTGGAAAACTGGTGTCACTCTCGGAACAAGAGCTAGTGGACTGTGACGTAAATACTGGGAACCAAGGTTGCAGTGGTGGATACATGGAAAATGCATTTGATTACATAAAAAAGCATGGACTCACCACTCAAGAAGATTATCCCTACACAGGATCAGATGGCACCTGCAATAAAGCTAAACAGAAAAAATATGCTGTGAAAATAGGCGGCTATGAAACAGTACCTGATAATGATGAAAAAAGCTTACAAGCTGCAGTTGCTCATCAACCTGTCTCGGTTGCAATTGACGCGGGTGGTTTTGCTATGCAACTCTACTCTTCCGGTATCTTCACTGGCCTGCTATGTGGTAAGAGTCTCAACCATGGAGTGACAGCAGTCGGATATGGAGAAGAAAATGGTAACAAGTACTGGATTGTGAAAAACTCATGGGGTCCTAACTGGGGTGAATCTGGTTATATCAGAATGACACGTGATTCTATTGATAAGGAAGGTGCTTGCGGCATTGCTATGATGTCAAGCTACCCTGTTAAGTCTCGAAAAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

348

Amino Acids

39.28

Weight (kDa)

8.38

Isoelectric Point (pI)

29.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 46 - 102 1.5e-13 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 127 - 343 4.4e-85 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 674
Acc36I ACCTGC 1 cut(s) 674
AccB1I GGYRCC 1 cut(s) 662
AccII CGCG 1 cut(s) 791
AciI CCGC 4 cut(s) 474, 708, 791, 999
AclI AACGTT 1 cut(s) 219
AclWI GGATC 1 cut(s) 661
AcsI RAATTY 2 cut(s) 204, 278
AcvI CACGTG 1 cut(s) 971
AfaI GTAC 4 cut(s) 85, 137, 723, 908
AfiI CCNNNNNNNGG 2 cut(s) 650, 941
AflIII ACRYGT 1 cut(s) 968
AjuI GAANNNNNNNTTGG 2 cut(s) 183, 215
AluBI AGCT 8 cut(s) 226, 332, 450, 535, 677, 744, 752, 1020
AluI AGCT 8 cut(s) 226, 332, 450, 535, 677, 744, 752, 1020
Alw26I GTCTC 4 cut(s) 78, 778, 860, 1038
AlwI GGATC 1 cut(s) 661
AlwNI CAGNNNCTG 1 cut(s) 725
AoxI GGCC 3 cut(s) 436, 484, 835
ApeKI GCWGC 1 cut(s) 752
ApoI RAATTY 2 cut(s) 204, 278
ArsI GACNNNNNNTTYG 2 cut(s) 263, 295
Asp700I GAANNNNTTC 1 cut(s) 486
AspS9I GGNCC 2 cut(s) 430, 932
AsuHPI GGTGA 2 cut(s) 616, 956
AvaII GGWCC 2 cut(s) 430, 932
BanI GGYRCC 1 cut(s) 662
BarI GAAGNNNNNNTAC 2 cut(s) 119, 151
BbrPI CACGTG 1 cut(s) 971
BbvI GCAGC 1 cut(s) 739
BccI CCATC 1 cut(s) 653
BciVI GTATCC 1 cut(s) 575
BcoDI GTCTC 4 cut(s) 78, 778, 860, 1038
BfaI CTAG 1 cut(s) 536
BfmI CTRYAG 3 cut(s) 387, 465, 753
BfuAI ACCTGC 1 cut(s) 674
BfuI GTATCC 1 cut(s) 575
BisI GCNGC 4 cut(s) 474, 709, 753, 1000
BlsI GCNGC 4 cut(s) 475, 710, 754, 1001
BmcAI AGTACT 1 cut(s) 908
Bme18I GGWCC 2 cut(s) 430, 932
BmgT120I GGNCC 2 cut(s) 430, 932
BmiI GGNNCC 4 cut(s) 431, 563, 664, 933
BmrI ACTGGG 2 cut(s) 567, 949
BmuI ACTGGG 2 cut(s) 567, 949
BoxI GACNNNNGTC 1 cut(s) 875
BplI GAGNNNNNCTC 2 cut(s) 403, 435
Bpu10I CCTNAGC 1 cut(s) 20
BpuEI CTTGAG 1 cut(s) 615
BsaAI YACGTR 1 cut(s) 971
BsaJI CCNNGG 3 cut(s) 433, 565, 862
BsaWI WCCGGW 2 cut(s) 421, 821
Bsc4I CCNNNNNNNGG 2 cut(s) 650, 941
Bse1I ACTGG 8 cut(s) 30, 211, 508, 516, 562, 838, 914, 944
Bse3DI GCAATG 1 cut(s) 1002
BseDI CCNNGG 3 cut(s) 433, 565, 862
BseLI CCNNNNNNNGG 2 cut(s) 650, 941
BseMI GCAATG 1 cut(s) 1002
BseNI ACTGG 8 cut(s) 30, 211, 508, 516, 562, 838, 914, 944
BseRI GAGGAG 1 cut(s) 197
BseXI GCAGC 1 cut(s) 739
Bsh1236I CGCG 1 cut(s) 791
Bsh1285I CGRYCG 1 cut(s) 163
BshFI GGCC 3 cut(s) 438, 486, 837
BshNI GGYRCC 1 cut(s) 662
BsiEI CGRYCG 1 cut(s) 163
BsiSI CCGG 2 cut(s) 422, 822
BslFI GGGAC 1 cut(s) 443
BslI CCNNNNNNNGG 2 cut(s) 650, 941
BsmAI GTCTC 4 cut(s) 78, 778, 860, 1038
BsmBI CGTCTC 1 cut(s) 78
BsmFI GGGAC 1 cut(s) 443
BsmI GAATGC 1 cut(s) 458
BsnI GGCC 3 cut(s) 438, 486, 837
Bsp143I GATC 1 cut(s) 653
Bsp19I CCATGG 1 cut(s) 862
BspACI CCGC 4 cut(s) 474, 708, 791, 999
BspANI GGCC 3 cut(s) 438, 486, 837
BspFNI CGCG 1 cut(s) 791
BspLI GGNNCC 4 cut(s) 431, 563, 664, 933
BspMAI CTGCAG 2 cut(s) 469, 757
BspMI ACCTGC 1 cut(s) 674
BspPI GGATC 1 cut(s) 661
BspT107I GGYRCC 1 cut(s) 662
BsrDI GCAATG 1 cut(s) 1002
BsrI ACTGG 8 cut(s) 30, 211, 508, 516, 562, 838, 914, 944
BssECI CCNNGG 3 cut(s) 433, 565, 862
BssMI GATC 1 cut(s) 653
BssT1I CCWWGG 3 cut(s) 433, 565, 862
Bst4CI ACNGT 3 cut(s) 391, 545, 721
Bst6I CTCTTC 1 cut(s) 823
BstAPI GCANNNNNTGC 1 cut(s) 464
BstBAI YACGTR 1 cut(s) 971
BstC8I GCNNGC 2 cut(s) 839, 997
BstDEI CTNAG 2 cut(s) 20, 298
BstDSI CCRYGG 1 cut(s) 862
BstFNI CGCG 1 cut(s) 791
BstKTI GATC 1 cut(s) 656
BstMAI GTCTC 4 cut(s) 78, 778, 860, 1038
BstMBI GATC 1 cut(s) 653
BstMCI CGRYCG 1 cut(s) 163
BstMWI GCNNNNNNNGC 4 cut(s) 464, 473, 758, 788
BstNSI RCATGY 1 cut(s) 84
BstPAI GACNNNNGTC 1 cut(s) 875
BstSFI CTRYAG 3 cut(s) 387, 465, 753
BstUI CGCG 1 cut(s) 791
BstV1I GCAGC 1 cut(s) 739
BsuI GTATCC 1 cut(s) 575
BsuRI GGCC 3 cut(s) 438, 486, 837
BtgI CCRYGG 1 cut(s) 862
BtsI GCAGTG 1 cut(s) 580
BtsIMutI CAGTG 4 cut(s) 267, 396, 580, 831
BveI ACCTGC 1 cut(s) 674
Cac8I GCNNGC 2 cut(s) 839, 997
CaiI CAGNNNCTG 1 cut(s) 725
Cfr13I GGNCC 2 cut(s) 430, 932
CseI GACGC 2 cut(s) 64, 797
Csp6I GTAC 4 cut(s) 84, 136, 722, 907
CspCI CAANNNNNGTGG 2 cut(s) 372, 407
CviAII CATG 7 cut(s) 81, 124, 289, 586, 617, 863, 927
CviQI GTAC 4 cut(s) 84, 136, 722, 907
DdeI CTNAG 2 cut(s) 20, 298
DpnI GATC 1 cut(s) 655
DpnII GATC 1 cut(s) 653
Eam1104I CTCTTC 1 cut(s) 823
EarI CTCTTC 1 cut(s) 823
Eco130I CCWWGG 3 cut(s) 433, 565, 862
Eco147I AGGCCT 1 cut(s) 486
Eco47I GGWCC 2 cut(s) 430, 932
Eco72I CACGTG 1 cut(s) 971
EcoO109I RGGNCCY 1 cut(s) 932
EcoT14I CCWWGG 3 cut(s) 433, 565, 862
EcoT22I ATGCAT 1 cut(s) 598
ErhI CCWWGG 3 cut(s) 433, 565, 862
Esp3I CGTCTC 1 cut(s) 78
FaeI CATG 7 cut(s) 84, 127, 292, 589, 620, 866, 930
FalI AAGNNNNNCTT 2 cut(s) 362, 394
FaqI GGGAC 1 cut(s) 443
FatI CATG 7 cut(s) 80, 123, 288, 585, 616, 862, 926
FauI CCCGC 1 cut(s) 784
Fnu4HI GCNGC 4 cut(s) 474, 709, 753, 1000
Fsp4HI GCNGC 4 cut(s) 474, 709, 753, 1000
FspBI CTAG 1 cut(s) 536
GluI GCNGC 4 cut(s) 474, 709, 753, 1000
HaeIII GGCC 3 cut(s) 438, 486, 837
HapII CCGG 2 cut(s) 422, 822
HgaI GACGC 2 cut(s) 64, 797
Hin1II CATG 7 cut(s) 84, 127, 292, 589, 620, 866, 930
HindIII AAGCTT 1 cut(s) 742
HinfI GANTC 4 cut(s) 621, 853, 947, 974
HpaII CCGG 2 cut(s) 422, 822
HphI GGTGA 2 cut(s) 616, 956
Hpy166II GTNNAC 3 cut(s) 306, 326, 541
Hpy188I TCNGA 4 cut(s) 526, 658, 881, 962
Hpy188III TCNNGA 4 cut(s) 52, 491, 632, 1035
Hpy8I GTNNAC 3 cut(s) 306, 326, 541
HpyAV CCTTC 5 cut(s) 121, 367, 476, 497, 983
HpyCH4III ACNGT 3 cut(s) 391, 545, 721
HpyCH4IV ACGT 3 cut(s) 219, 549, 970
HpyCH4V TGCA 8 cut(s) 284, 467, 573, 596, 669, 755, 782, 808
HpyF10VI GCNNNNNNNGC 4 cut(s) 464, 473, 758, 788
HpyF3I CTNAG 2 cut(s) 20, 298
HpySE526I ACGT 3 cut(s) 219, 549, 970
Hsp92II CATG 7 cut(s) 84, 127, 292, 589, 620, 866, 930
Kzo9I GATC 1 cut(s) 653
LmnI GCTCC 1 cut(s) 411
Lsp1109I GCAGC 1 cut(s) 739
MaeI CTAG 1 cut(s) 536
MaeII ACGT 3 cut(s) 219, 549, 970
MaeIII GTNAC 5 cut(s) 415, 516, 545, 868, 899
MalI GATC 1 cut(s) 655
MboI GATC 1 cut(s) 653
MboII GAAGA 6 cut(s) 177, 382, 647, 810, 820, 902
MfeI CAATTG 1 cut(s) 783
MluCI AATT 3 cut(s) 204, 278, 783
MlyI GAGTC 2 cut(s) 615, 862
MmeI TCCRAC 2 cut(s) 240, 859
MnlI CCTC 2 cut(s) 126, 175
Mph1103I ATGCAT 1 cut(s) 598
MroXI GAANNNNTTC 1 cut(s) 486
MseI TTAA 1 cut(s) 1029
MslI CAYNNNNRTG 1 cut(s) 1007
MspA1I CMGCKG 1 cut(s) 476
MspI CCGG 2 cut(s) 422, 822
MunI CAATTG 1 cut(s) 783
Mva1269I GAATGC 1 cut(s) 458
MvnI CGCG 1 cut(s) 791
MwoI GCNNNNNNNGC 4 cut(s) 464, 473, 758, 788
NcoI CCATGG 1 cut(s) 862
NdeII GATC 1 cut(s) 653
NlaIII CATG 7 cut(s) 84, 127, 292, 589, 620, 866, 930
NlaIV GGNNCC 4 cut(s) 431, 563, 664, 933
NmuCI GTSAC 3 cut(s) 516, 545, 868
NsiI ATGCAT 1 cut(s) 598
NspI RCATGY 1 cut(s) 84
PaqCI CACCTGC 1 cut(s) 674
PceI AGGCCT 1 cut(s) 486
PctI GAATGC 1 cut(s) 458
PdmI GAANNNNTTC 1 cut(s) 486
PfeI GAWTC 2 cut(s) 947, 974
PkrI GCNGC 4 cut(s) 475, 710, 754, 1001
PleI GAGTC 2 cut(s) 615, 861
PmaCI CACGTG 1 cut(s) 971
PmlI CACGTG 1 cut(s) 971
PpsI GAGTC 2 cut(s) 615, 861
Ppu21I YACGTR 1 cut(s) 971
PpuMI RGGWCCY 1 cut(s) 932
PshAI GACNNNNGTC 1 cut(s) 875
Psp1406I AACGTT 1 cut(s) 219
Psp5II RGGWCCY 1 cut(s) 932
PspCI CACGTG 1 cut(s) 971
PspN4I GGNNCC 4 cut(s) 431, 563, 664, 933
PspPI GGNCC 2 cut(s) 430, 932
PspPPI RGGWCCY 1 cut(s) 932
PstI CTGCAG 2 cut(s) 469, 757
PstNI CAGNNNCTG 1 cut(s) 725
RsaI GTAC 4 cut(s) 85, 137, 723, 908
RsaNI GTAC 4 cut(s) 84, 136, 722, 907
RseI CAYNNNNRTG 1 cut(s) 1007
SaqAI TTAA 1 cut(s) 1029
SatI GCNGC 4 cut(s) 474, 709, 753, 1000
Sau3AI GATC 1 cut(s) 653
Sau96I GGNCC 2 cut(s) 430, 932
ScaI AGTACT 1 cut(s) 908
SchI GAGTC 2 cut(s) 615, 862
SfcI CTRYAG 3 cut(s) 387, 465, 753
SinI GGWCC 2 cut(s) 430, 932
SmiMI CAYNNNNRTG 1 cut(s) 1007
SmlI CTYRAG 1 cut(s) 630
SmoI CTYRAG 1 cut(s) 630
Sse9I AATT 3 cut(s) 204, 278, 783
SseBI AGGCCT 1 cut(s) 486
SsiI CCGC 4 cut(s) 474, 708, 791, 999
SspMI CTAG 1 cut(s) 536
StuI AGGCCT 1 cut(s) 486
StyI CCWWGG 3 cut(s) 433, 565, 862
TaaI ACNGT 3 cut(s) 391, 545, 721
TaiI ACGT 3 cut(s) 222, 552, 973
TaqI TCGA 2 cut(s) 231, 1036
TaqII GACCGA 1 cut(s) 177
TasI AATT 3 cut(s) 204, 278, 783
TatI WGTACW 2 cut(s) 83, 906
TauI GCSGC 3 cut(s) 476, 711, 1002
TfiI GAWTC 2 cut(s) 947, 974
Tru1I TTAA 1 cut(s) 1029
Tru9I TTAA 1 cut(s) 1029
TscAI CASTG 4 cut(s) 274, 396, 580, 838
TseFI GTSAC 3 cut(s) 516, 545, 868
TseI GCWGC 1 cut(s) 752
Tsp45I GTSAC 3 cut(s) 516, 545, 868
TspDTI ATGAA 4 cut(s) 140, 226, 729, 750
TspRI CASTG 4 cut(s) 274, 396, 580, 838
VpaK11BI GGWCC 2 cut(s) 430, 932
XapI RAATTY 2 cut(s) 204, 278
XceI RCATGY 1 cut(s) 84
XcmI CCANNNNNNNNNTGG 2 cut(s) 440, 572
XmnI GAANNNNTTC 1 cut(s) 486
XspI CTAG 1 cut(s) 536
ZrmI AGTACT 1 cut(s) 908
Zsp2I ATGCAT 1 cut(s) 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.