Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0000409

Physical Location & Seq

Genomic Coordinates Reverse (-)

488057 .. 489737

Gene Structure 1681 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1003300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1176 bp

ATGGTTACAGTCGAGGAAGTTCGCAAGGCTCAACGGGCGGAGGGTCCAGCCACAGTCATGGCCATCGGGACAGCAACTCCTTCCAACTGTGTGGATCAGGCTACCTACCCCGACTACTACTTTCGTATCACCAACAGCGAGCACAAGGTTGAGCTCAAAGAAAAATTCCAGCGCATGTGCGACAAATCTATGATCAAGAAACGTTATATGTACTTGACTGAAGAAATTTTAAAAGAGAACCCAAGTGTGTGCGAGTACATGGCTCCTTCAATTGATGCAAGGCAGGACATGGTGGTTGTGGAAGTCCCAAAACTTGGCAAAGAGGCTGCCACCAAAGCCATCAAGGAATGGGGACAGCCCAAGTCCAAAATCACCCACTTGGTCTTTTGCACCACCAGCGGTGTCGACATGCCTGGCGCCGACTACCAACTCACCAAGCTCTTGGGCCTCCGCCCCTCCGTCAAGCGCCTCATGATGTACCAGCAAGGGTGCTTCGCCGGTGGGACGGTCCTCCGTTTGGCCAAGGACTTGGCCGAAAACAACAAGGGTGCACGTGTTCTTGTTGTGTGCTCTGAGATCACCGCGGTTACCTTCCGTGGGCCTAGTGACACCCACCTTGATAGTCTTGTGGGCCAAGCTTTGTTTGGCGACGGTGCAGCGGCCGTAATCATTGGTGCGGATCCAGTGCCCGAAGTCGAGAAGCCCTTGTTTGAATTGGTGTCGGCGGCACAAACCATTCTCCCCGACAGTGATGGGGCTATCGACGGACATCTCCGTGAAGTAGGGCTTACATTTCACCTTCTCAAGGATGTTCCCGGGCTTATTTCGAAGAACATCGAAAAGAGCCTTAATGAGGCTTTCAAGCCTATTGGGATTTCGGACTGGAACTCACTCTTCTGGATTGCACACCCAGGTGGCCCTGCTATTCTGGACCAAGTAGAGGCCAAGTTGGCATTGAAGCCGGAGAAACTAGAAGCAACAAGGCAAGTGTTGTCGGATTACGGTAACATGTCGAGTGCTTGTGTGCTTTTTATTTTGGACGAGGTCAGGAGGAAGTCCGCCGAGAAAGGACTCAAAACGACCGGGGAGGGACTGGAGTGGGGTGTGCTTTTCGGATTTGGGCCCGGCCTCACGGTGGAGACCGTCGTGCTTCACAGCGTGGGTTTAACGGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009058 GO:0009812 GO:0009813 GO:0016210 GO:0016740 GO:0016746 GO:0016747 GO:0071704 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00941 ko01100 ko01110 ko04712 map00941 map01100 map01110 map04712

Pfam Domains

Protein Families

Chal_sti_synt_C Chal_sti_synt_N

Protein Analysis

392

Amino Acids

42.61

Weight (kDa)

5.9

Isoelectric Point (pI)

32.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Chal_sti_synt_N	PF00195	5 - 228	1.4e-125	Chalcone and stilbene synthases, N-terminal domain
Chal_sti_synt_C	PF02797	238 - 387	1.8e-72	Chalcone and stilbene synthases, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000409)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13930
fragaria_vesca	FvH4_2g28040 FvH4_2g28050 FvH4_7g01160 FvH4_7g01160 FvH4_7g01160 FvH4_7g01160 FvH4_7g01160 FvH4_7g01160 FvH4_7g01160
malus_domestica	MD04G1003000.v1.1 MD04G1003300.v1.1 MD04G1003400.v1.1 MD04G1111500.v1.1 MD08G1116200.v1.1 MD13G1285100.v1.1 MD14G1160800.v1.1 MD14G1160900.v1.1 MD15G1131700.v1.1 MD15G1132000.v1.1 MD15G1132100.v1.1 MD15G1132200.v1.1 MD15G1132300.v1.1
prunus_persica	Prupe.1G002900_v2.0.a1 Prupe.1G002900_v2.0.a1 Prupe.1G003000_v2.0.a1 Prupe.4G252100_v2.0.a1 Prupe.4G252800_v2.0.a1 Prupe.4G253000_v2.0.a1 Prupe.4G253100_v2.0.a1 Prupe.I005700_v2.0.a1 Prupe.I005700_v2.0.a1 Prupe.I005800_v2.0.a1
pyrus_communis	pycom04g00310 pycom04g00320 pycom04g00350 pycom13g10200 pycom15g11900 pycom15g11910 pycom15g11920 pycom15g11930 pycom15g11940 pycom420g00730
rosa_chinensis	RchiOBHm_Chr1g0316441 RchiOBHm_Chr1g0316451 RchiOBHm_Chr1g0316461 RchiOBHm_Chr4g0399981 RchiOBHm_Chr6g0296951 RchiOBHm_Chr6g0297001 RchiOBHm_Chr6g0297011
rosa_laevigata	RLG00000009221 RLG00000011646 RLG00000030671 RLG00000030673
rosa_multiflora	Rmu_co8031452.1_g000001 Rmu_co8152518.1_g000001 Rmu_sc0000081.1_g000010 Rmu_sc0004672.1_g000001 Rmu_sc0006453.1_g000028 Rmu_sc0006790.1_g000004 Rmu_sc0007272.1_g000004 Rmu_sc0023107.1_g000002 Rmu_sc0023714.1_g000001
rosa_roxburghii	Rroxscaffold_4G00330980 Rroxscaffold_4G00330990 Rroxscaffold_4G00331000 Rroxscaffold_7G00171030
rosa_rugosa	Rorug01G0010500 Rorug01G0010600 Rorug01G0011000 Rorug03G0299400 Rorug03G0299500 Rorug04G0022100 Rorug05G0381700 Rorug06G0267700
rosa_samantha	Rh1AG020500 Rh1BG015600 Rh1BG015800 Rh1CG018400 Rh1CG018600 Rh1CG018800 Rh1DG015500 Rh1DG015700 Rh1DG015900 Rh4AG097700 Rh4BG095700 Rh4CG106900 Rh4DG092500 Rh6AG378300 Rh6BG386300 Rh6CG392100 Rh6CG392300 Rh6DG379000
rosa_wichuraiana	Rw0G010230 Rw1G001330 Rw1G001340 Rw4G008110 Rw4G013630 Rw6G033070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	416
AccB7I	CCANNNNNTGG	1 cut(s)	314
AccI	GTMKAC	1 cut(s)	405
AccII	CGCG	1 cut(s)	584
AciI	CCGC	9 cut(s)	38, 399, 451, 582, 584, 659, 677, 725, 1059
AclI	AACGTT	1 cut(s)	202
AclWI	GGATC	3 cut(s)	102, 674, 687
AcoI	YGGCCR	4 cut(s)	60, 519, 531, 660
AcsI	RAATTY	2 cut(s)	164, 225
AcuI	CTGAAG	1 cut(s)	240
AcvI	CACGTG	1 cut(s)	554
AcyI	GRCGYC	1 cut(s)	417
AdeI	CACNNNGTG	1 cut(s)	1159
AfaI	GTAC	3 cut(s)	212, 257, 479
AfiI	CCNNNNNNNGG	7 cut(s)	314, 517, 597, 805, 853, 940, 1135
AflIII	ACRYGT	2 cut(s)	553, 1008
AgsI	TTSAA	4 cut(s)	270, 713, 862, 958
AjnI	CCWGG	2 cut(s)	412, 910
AleI	CACNNNNGTG	1 cut(s)	912
AluBI	AGCT	3 cut(s)	154, 439, 638
AluI	AGCT	3 cut(s)	154, 439, 638
Alw21I	GWGCWC	4 cut(s)	144, 156, 553, 572
Alw26I	GTCTC	1 cut(s)	1133
Alw44I	GTGCAC	1 cut(s)	549
AlwI	GGATC	3 cut(s)	102, 674, 687
Ama87I	CYCGRG	1 cut(s)	815
ApaI	GGGCCC	1 cut(s)	1125
ApaLI	GTGCAC	1 cut(s)	549
ApeKI	GCWGC	2 cut(s)	326, 656
ApoI	RAATTY	2 cut(s)	164, 225
AspLEI	GCGC	3 cut(s)	174, 419, 468
AspS9I	GGNCC	9 cut(s)	44, 445, 508, 599, 631, 917, 931, 1121, 1122
AsuC2I	CCSGG	4 cut(s)	816, 817, 1084, 1125
AsuHPI	GGTGA	5 cut(s)	121, 364, 424, 571, 788
AsuII	TTCGAA	1 cut(s)	827
AvaI	CYCGRG	1 cut(s)	815
AvaII	GGWCC	3 cut(s)	44, 508, 931
BaeGI	GKGCMC	3 cut(s)	553, 690, 1125
BaeI	ACNNNNGTAYC	2 cut(s)	109, 142
BalI	TGGCCA	2 cut(s)	62, 521
BamHI	GGATCC	1 cut(s)	679
BanI	GGYRCC	1 cut(s)	416
BanII	GRGCYC	2 cut(s)	156, 1125
BbrPI	CACGTG	1 cut(s)	554
Bbv12I	GWGCWC	4 cut(s)	144, 156, 553, 572
BbvI	GCAGC	2 cut(s)	313, 668
BccI	CCATC	3 cut(s)	71, 347, 746
BceAI	ACGGC	1 cut(s)	647
BciT130I	CCWGG	2 cut(s)	414, 912
BclI	TGATCA	1 cut(s)	192
BcnI	CCSGG	4 cut(s)	816, 817, 1084, 1125
BcoDI	GTCTC	1 cut(s)	1133
BfaI	CTAG	2 cut(s)	603, 971
BfoI	RGCGCY	2 cut(s)	420, 469
BglI	GCCNNNNNGGC	1 cut(s)	950
BisI	GCNGC	4 cut(s)	327, 657, 660, 726
BlsI	GCNGC	4 cut(s)	328, 658, 661, 727
Bme1390I	CCNGG	6 cut(s)	414, 816, 817, 912, 1084, 1125
Bme18I	GGWCC	3 cut(s)	44, 508, 931
BmeT110I	CYCGRG	1 cut(s)	815
BmgT120I	GGNCC	9 cut(s)	44, 445, 508, 599, 631, 917, 931, 1121, 1122
BmiI	GGNNCC	5 cut(s)	45, 264, 418, 681, 1123
BmrFI	CCNGG	6 cut(s)	414, 816, 817, 912, 1084, 1125
BmsI	GCATC	1 cut(s)	265
BpmI	CTGGAG	1 cut(s)	1115
Bpu14I	TTCGAA	1 cut(s)	827
BpuEI	CTTGAG	1 cut(s)	788
BpuMI	CCSGG	4 cut(s)	816, 817, 1084, 1125
BsaAI	YACGTR	1 cut(s)	554
BsaHI	GRCGYC	1 cut(s)	417
BsaI	GGTCTC	1 cut(s)	1133
BsaJI	CCNNGG	6 cut(s)	522, 582, 595, 815, 910, 1083
BsaXI	ACNNNNNCTCC	2 cut(s)	61, 91
Bsc4I	CCNNNNNNNGG	7 cut(s)	314, 517, 597, 805, 853, 940, 1135
Bse118I	RCCGGY	1 cut(s)	497
Bse1I	ACTGG	3 cut(s)	683, 887, 1098
BseBI	CCWGG	2 cut(s)	414, 912
BseDI	CCNNGG	6 cut(s)	522, 582, 595, 815, 910, 1083
BseGI	GGATG	1 cut(s)	814
BseLI	CCNNNNNNNGG	7 cut(s)	314, 517, 597, 805, 853, 940, 1135
BseMII	CTCAG	1 cut(s)	564
BseNI	ACTGG	3 cut(s)	683, 887, 1098
BseSI	GKGCMC	3 cut(s)	553, 690, 1125
BseX3I	CGGCCG	1 cut(s)	660
BseXI	GCAGC	2 cut(s)	313, 668
BsgI	GTGCAG	1 cut(s)	675
Bsh1236I	CGCG	1 cut(s)	584
Bsh1285I	CGRYCG	2 cut(s)	663, 1083
BshNI	GGYRCC	1 cut(s)	416
BsiEI	CGRYCG	2 cut(s)	663, 1083
BsiHKAI	GWGCWC	4 cut(s)	144, 156, 553, 572
BsiHKCI	CYCGRG	1 cut(s)	815
BsiSI	CCGG	5 cut(s)	498, 816, 962, 1083, 1125
BslFI	GGGAC	5 cut(s)	82, 290, 366, 517, 1104
BslI	CCNNNNNNNGG	7 cut(s)	314, 517, 597, 805, 853, 940, 1135
BsmAI	GTCTC	1 cut(s)	1133
BsmFI	GGGAC	5 cut(s)	82, 290, 366, 517, 1104
Bso31I	GGTCTC	1 cut(s)	1133
BsoBI	CYCGRG	1 cut(s)	815
Bsp119I	TTCGAA	1 cut(s)	827
Bsp120I	GGGCCC	1 cut(s)	1121
Bsp1286I	GDGCHC	6 cut(s)	144, 156, 553, 572, 690, 1125
Bsp143I	GATC	4 cut(s)	94, 192, 576, 679
BspACI	CCGC	9 cut(s)	38, 399, 451, 582, 584, 659, 677, 725, 1059
BspCNI	CTCAG	1 cut(s)	565
BspFNI	CGCG	1 cut(s)	584
BspHI	TCATGA	1 cut(s)	471
BspLI	GGNNCC	5 cut(s)	45, 264, 418, 681, 1123
BspPI	GGATC	3 cut(s)	102, 674, 687
BspT104I	TTCGAA	1 cut(s)	827
BspT107I	GGYRCC	1 cut(s)	416
BspTNI	GGTCTC	1 cut(s)	1133
BsrFI	RCCGGY	1 cut(s)	497
BsrI	ACTGG	3 cut(s)	683, 887, 1098
BssAI	RCCGGY	1 cut(s)	497
BssECI	CCNNGG	6 cut(s)	522, 582, 595, 815, 910, 1083
BssMI	GATC	4 cut(s)	94, 192, 576, 679
BssNI	GRCGYC	1 cut(s)	417
BssT1I	CCWWGG	1 cut(s)	522
Bst2UI	CCWGG	2 cut(s)	414, 912
Bst4CI	ACNGT	9 cut(s)	10, 55, 89, 508, 653, 749, 1004, 1135, 1144
Bst6I	CTCTTC	1 cut(s)	899
BstACI	GRCGYC	1 cut(s)	417
BstBAI	YACGTR	1 cut(s)	554
BstBI	TTCGAA	1 cut(s)	827
BstC8I	GCNNGC	1 cut(s)	140
BstDEI	CTNAG	1 cut(s)	573
BstDSI	CCRYGG	2 cut(s)	582, 595
BstEII	GGTNACC	1 cut(s)	586
BstENI	CCTNNNNNAGG	2 cut(s)	803, 851
BstF5I	GGATG	1 cut(s)	814
BstFNI	CGCG	1 cut(s)	584
BstH2I	RGCGCY	2 cut(s)	420, 469
BstHHI	GCGC	3 cut(s)	174, 419, 468
BstKTI	GATC	4 cut(s)	97, 195, 579, 682
BstMAI	GTCTC	1 cut(s)	1133
BstMBI	GATC	4 cut(s)	94, 192, 576, 679
BstMCI	CGRYCG	2 cut(s)	663, 1083
BstMWI	GCNNNNNNNGC	4 cut(s)	35, 335, 396, 950
BstNI	CCWGG	2 cut(s)	414, 912
BstNSI	RCATGY	3 cut(s)	178, 412, 1012
BstPI	GGTNACC	1 cut(s)	586
BstSCI	CCNGG	6 cut(s)	412, 814, 815, 910, 1082, 1123
BstSLI	GKGCMC	3 cut(s)	553, 690, 1125
BstUI	CGCG	1 cut(s)	584
BstV1I	GCAGC	2 cut(s)	313, 668
BstX2I	RGATCY	1 cut(s)	679
BstXI	CCANNNNNNTGG	4 cut(s)	58, 91, 442, 529
BstYI	RGATCY	1 cut(s)	679
BstZI	CGGCCG	1 cut(s)	660
BtgI	CCRYGG	2 cut(s)	582, 595
BtsCI	GGATG	1 cut(s)	814
BtsIMutI	CAGTG	2 cut(s)	690, 754
Cac8I	GCNNGC	1 cut(s)	140
CciI	TCATGA	1 cut(s)	471
CfoI	GCGC	3 cut(s)	174, 419, 468
Cfr10I	RCCGGY	1 cut(s)	497
Cfr13I	GGNCC	9 cut(s)	44, 445, 508, 599, 631, 917, 931, 1121, 1122
Cfr42I	CCGCGG	1 cut(s)	585
Cfr9I	CCCGGG	1 cut(s)	815
Csp6I	GTAC	3 cut(s)	211, 256, 478
CviAII	CATG	7 cut(s)	58, 175, 259, 289, 409, 472, 1009
CviQI	GTAC	3 cut(s)	211, 256, 478
DdeI	CTNAG	1 cut(s)	573
DinI	GGCGCC	1 cut(s)	418
DpnI	GATC	4 cut(s)	96, 194, 578, 681
DpnII	GATC	4 cut(s)	94, 192, 576, 679
DraI	TTTAAA	1 cut(s)	231
DraIII	CACNNNGTG	1 cut(s)	1159
EaeI	YGGCCR	4 cut(s)	60, 519, 531, 660
EagI	CGGCCG	1 cut(s)	660
Eam1104I	CTCTTC	1 cut(s)	899
EarI	CTCTTC	1 cut(s)	899
EciI	GGCGGA	3 cut(s)	53, 440, 1048
Ecl136II	GAGCTC	1 cut(s)	154
EclXI	CGGCCG	1 cut(s)	660
Eco130I	CCWWGG	1 cut(s)	522
Eco24I	GRGCYC	2 cut(s)	156, 1125
Eco31I	GGTCTC	1 cut(s)	1133
Eco47I	GGWCC	3 cut(s)	44, 508, 931
Eco52I	CGGCCG	1 cut(s)	660
Eco53kI	GAGCTC	1 cut(s)	154
Eco57I	CTGAAG	1 cut(s)	240
Eco72I	CACGTG	1 cut(s)	554
Eco88I	CYCGRG	1 cut(s)	815
Eco91I	GGTNACC	1 cut(s)	586
EcoICRI	GAGCTC	1 cut(s)	154
EcoNI	CCTNNNNNAGG	2 cut(s)	803, 851
EcoO65I	GGTNACC	1 cut(s)	586
EcoRII	CCWGG	2 cut(s)	412, 910
EcoT14I	CCWWGG	1 cut(s)	522
EcoT38I	GRGCYC	2 cut(s)	156, 1125
EgeI	GGCGCC	1 cut(s)	418
EheI	GGCGCC	1 cut(s)	418
ErhI	CCWWGG	1 cut(s)	522
FaeI	CATG	7 cut(s)	61, 178, 262, 292, 412, 475, 1012
FalI	AAGNNNNNCTT	2 cut(s)	771, 803
FaqI	GGGAC	5 cut(s)	82, 290, 366, 517, 1104
FatI	CATG	7 cut(s)	57, 174, 258, 288, 408, 471, 1008
FbaI	TGATCA	1 cut(s)	192
FblI	GTMKAC	1 cut(s)	405
Fnu4HI	GCNGC	4 cut(s)	327, 657, 660, 726
FokI	GGATG	1 cut(s)	821
FriOI	GRGCYC	2 cut(s)	156, 1125
Fsp4HI	GCNGC	4 cut(s)	327, 657, 660, 726
FspBI	CTAG	2 cut(s)	603, 971
GlaI	GCGC	3 cut(s)	173, 418, 467
GluI	GCNGC	4 cut(s)	327, 657, 660, 726
GsuI	CTGGAG	1 cut(s)	1115
HaeII	RGCGCY	2 cut(s)	420, 469
HapII	CCGG	5 cut(s)	498, 816, 962, 1083, 1125
HhaI	GCGC	3 cut(s)	174, 419, 468
Hin1I	GRCGYC	1 cut(s)	417
Hin1II	CATG	7 cut(s)	61, 178, 262, 292, 412, 475, 1012
Hin6I	GCGC	3 cut(s)	172, 417, 466
HinP1I	GCGC	3 cut(s)	172, 417, 466
HincII	GTYRAC	1 cut(s)	406
HindII	GTYRAC	1 cut(s)	406
HindIII	AAGCTT	1 cut(s)	636
HinfI	GANTC	1 cut(s)	1071
HpaII	CCGG	5 cut(s)	498, 816, 962, 1083, 1125
HphI	GGTGA	5 cut(s)	121, 364, 424, 571, 788
Hpy166II	GTNNAC	2 cut(s)	406, 551
Hpy188I	TCNGA	4 cut(s)	574, 880, 997, 1115
Hpy188III	TCNNGA	7 cut(s)	67, 196, 472, 697, 898, 929, 1048
Hpy8I	GTNNAC	2 cut(s)	406, 551
Hpy99I	CGWCG	3 cut(s)	653, 767, 1148
HpyAV	CCTTC	4 cut(s)	90, 276, 601, 809
HpyCH4III	ACNGT	9 cut(s)	10, 55, 89, 508, 653, 749, 1004, 1135, 1144
HpyCH4IV	ACGT	2 cut(s)	202, 553
HpyCH4V	TGCA	5 cut(s)	278, 390, 551, 656, 905
HpyF10VI	GCNNNNNNNGC	4 cut(s)	35, 335, 396, 950
HpyF3I	CTNAG	1 cut(s)	573
HpySE526I	ACGT	2 cut(s)	202, 553
Hsp92I	GRCGYC	1 cut(s)	417
Hsp92II	CATG	7 cut(s)	61, 178, 262, 292, 412, 475, 1012
HspAI	GCGC	3 cut(s)	172, 417, 466
KasI	GGCGCC	1 cut(s)	416
Ksp22I	TGATCA	1 cut(s)	192
KspI	CCGCGG	1 cut(s)	585
Kzo9I	GATC	4 cut(s)	94, 192, 576, 679
LmnI	GCTCC	1 cut(s)	268
Lsp1109I	GCAGC	2 cut(s)	313, 668
LweI	GCATC	1 cut(s)	265
MaeI	CTAG	2 cut(s)	603, 971
MaeII	ACGT	2 cut(s)	202, 553
MaeIII	GTNAC	4 cut(s)	4, 586, 605, 1004
MalI	GATC	4 cut(s)	96, 194, 578, 681
MboI	GATC	4 cut(s)	94, 192, 576, 679
MboII	GAAGA	3 cut(s)	233, 841, 886
MfeI	CAATTG	1 cut(s)	270
MflI	RGATCY	1 cut(s)	679
MhlI	GDGCHC	6 cut(s)	144, 156, 553, 572, 690, 1125
MlsI	TGGCCA	2 cut(s)	62, 521
MluCI	AATT	4 cut(s)	164, 225, 270, 713
MluNI	TGGCCA	2 cut(s)	62, 521
Mly113I	GGCGCC	1 cut(s)	417
MlyI	GAGTC	1 cut(s)	1065
MmeI	TCCRAC	2 cut(s)	108, 975
Mox20I	TGGCCA	2 cut(s)	62, 521
MscI	TGGCCA	2 cut(s)	62, 521
MseI	TTAA	3 cut(s)	230, 849, 1166
MslI	CAYNNNNRTG	3 cut(s)	56, 774, 912
Msp20I	TGGCCA	2 cut(s)	62, 521
MspA1I	CMGCKG	3 cut(s)	399, 584, 659
MspI	CCGG	5 cut(s)	498, 816, 962, 1083, 1125
MspR9I	CCNGG	6 cut(s)	414, 816, 817, 912, 1084, 1125
MunI	CAATTG	1 cut(s)	270
MvaI	CCWGG	2 cut(s)	414, 912
MvnI	CGCG	1 cut(s)	584
MwoI	GCNNNNNNNGC	4 cut(s)	35, 335, 396, 950
NarI	GGCGCC	1 cut(s)	417
NciI	CCSGG	4 cut(s)	816, 817, 1084, 1125
NdeII	GATC	4 cut(s)	94, 192, 576, 679
NlaIII	CATG	7 cut(s)	61, 178, 262, 292, 412, 475, 1012
NlaIV	GGNNCC	5 cut(s)	45, 264, 418, 681, 1123
NmeAIII	GCCGAG	1 cut(s)	1087
NmuCI	GTSAC	1 cut(s)	605
NspI	RCATGY	3 cut(s)	178, 412, 1012
NspV	TTCGAA	1 cut(s)	827
OliI	CACNNNNGTG	1 cut(s)	912
PagI	TCATGA	1 cut(s)	471
PciI	ACATGT	1 cut(s)	1008
PflFI	GACNNNGTC	1 cut(s)	1043
PflMI	CCANNNNNTGG	1 cut(s)	314
PkrI	GCNGC	4 cut(s)	328, 658, 661, 727
PleI	GAGTC	1 cut(s)	1065
PluTI	GGCGCC	1 cut(s)	420
PmaCI	CACGTG	1 cut(s)	554
PmlI	CACGTG	1 cut(s)	554
PpsI	GAGTC	1 cut(s)	1065
Ppu21I	YACGTR	1 cut(s)	554
PscI	ACATGT	1 cut(s)	1008
Psp124BI	GAGCTC	1 cut(s)	156
Psp1406I	AACGTT	1 cut(s)	202
Psp6I	CCWGG	2 cut(s)	412, 910
PspCI	CACGTG	1 cut(s)	554
PspEI	GGTNACC	1 cut(s)	586
PspGI	CCWGG	2 cut(s)	412, 910
PspN4I	GGNNCC	5 cut(s)	45, 264, 418, 681, 1123
PspOMI	GGGCCC	1 cut(s)	1121
PspPI	GGNCC	9 cut(s)	44, 445, 508, 599, 631, 917, 931, 1121, 1122
PsuI	RGATCY	1 cut(s)	679
PsyI	GACNNNGTC	1 cut(s)	1043
RsaI	GTAC	3 cut(s)	212, 257, 479
RsaNI	GTAC	3 cut(s)	211, 256, 478
RseI	CAYNNNNRTG	3 cut(s)	56, 774, 912
SacI	GAGCTC	1 cut(s)	156
SacII	CCGCGG	1 cut(s)	585
SalI	GTCGAC	1 cut(s)	404
SaqAI	TTAA	3 cut(s)	230, 849, 1166
SatI	GCNGC	4 cut(s)	327, 657, 660, 726
Sau3AI	GATC	4 cut(s)	94, 192, 576, 679
Sau96I	GGNCC	9 cut(s)	44, 445, 508, 599, 631, 917, 931, 1121, 1122
SchI	GAGTC	1 cut(s)	1065
ScrFI	CCNGG	6 cut(s)	414, 816, 817, 912, 1084, 1125
SduI	GDGCHC	6 cut(s)	144, 156, 553, 572, 690, 1125
SfaNI	GCATC	1 cut(s)	265
SfoI	GGCGCC	1 cut(s)	418
Sfr303I	CCGCGG	1 cut(s)	585
SfuI	TTCGAA	1 cut(s)	827
SgrAI	CRCCGGYG	1 cut(s)	497
SgrBI	CCGCGG	1 cut(s)	585
SinI	GGWCC	3 cut(s)	44, 508, 931
SmaI	CCCGGG	1 cut(s)	817
SmiMI	CAYNNNNRTG	3 cut(s)	56, 774, 912
SmlI	CTYRAG	1 cut(s)	803
SmoI	CTYRAG	1 cut(s)	803
Sse9I	AATT	4 cut(s)	164, 225, 270, 713
SsiI	CCGC	9 cut(s)	38, 399, 451, 582, 584, 659, 677, 725, 1059
SspDI	GGCGCC	1 cut(s)	416
SspMI	CTAG	2 cut(s)	603, 971
SstI	GAGCTC	1 cut(s)	156
StyD4I	CCNGG	6 cut(s)	412, 814, 815, 910, 1082, 1123
StyI	CCWWGG	1 cut(s)	522
TaaI	ACNGT	9 cut(s)	10, 55, 89, 508, 653, 749, 1004, 1135, 1144
TaiI	ACGT	2 cut(s)	205, 556
TaqI	TCGA	7 cut(s)	12, 405, 696, 762, 827, 837, 1013
TasI	AATT	4 cut(s)	164, 225, 270, 713
TatI	WGTACW	2 cut(s)	210, 255
TauI	GCSGC	2 cut(s)	662, 728
Tru1I	TTAA	3 cut(s)	230, 849, 1166
Tru9I	TTAA	3 cut(s)	230, 849, 1166
TscAI	CASTG	2 cut(s)	690, 754
TseFI	GTSAC	1 cut(s)	605
TseI	GCWGC	2 cut(s)	326, 656
Tsp45I	GTSAC	1 cut(s)	605
TspGWI	ACGGA	5 cut(s)	448, 503, 584, 764, 780
TspMI	CCCGGG	1 cut(s)	815
TspRI	CASTG	2 cut(s)	690, 754
Tth111I	GACNNNGTC	1 cut(s)	1043
Van91I	CCANNNNNTGG	1 cut(s)	314
VneI	GTGCAC	1 cut(s)	549
VpaK11BI	GGWCC	3 cut(s)	44, 508, 931
XagI	CCTNNNNNAGG	2 cut(s)	803, 851
XapI	RAATTY	2 cut(s)	164, 225
XceI	RCATGY	3 cut(s)	178, 412, 1012
XcmI	CCANNNNNNNNNTGG	1 cut(s)	641
XmaI	CCCGGG	1 cut(s)	815
XmiI	GTMKAC	1 cut(s)	405
XspI	CTAG	2 cut(s)	603, 971

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.