Rh6BG386300

Belongs to the chalcone stilbene synthases family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000409
Physical Location & Seq
Forward (+)
61429644 .. 61431025
1382 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG386300.1

Sequence Viewer

Length: 1164 bp
ATGGAGTCTCAGGCTAAAGAAGCAAAGGTTCCAGCCACAATACTAGCCATTGGGACTGCAAATCCAGTAAGCTGTTACTACCAAGAAGACTATCCCGATTTCTTGTTCAAAGTCACCAAAAGCGAGCACAAGACCGAATTAAAAGACAAGTTCAAACGCATATGTGAAAAGTCGATGGTAAAGAAGCGATATCTGGGAGTCACAGAAGAGAATCTAAAAGCCAACCCTAACATATGCAGCTACAAGGCTCCCTCACTCGACGCACGTCAAGACTTGCTGATTCACGAGGTCCCCAAACTCGGTAAAGAAGCGGCGTTGAAGGCCATCAGAGAATGGGGCCAACCCATTTCAAGCCTCACCCACCTCATCTTCTGCACAGCTTCCTGCGTCGACATGCCCGGTGCCGACTTTCAGCTCGTCAAGCTCCTCGGCCTAAATCCATCTATCAACCGGTTCATGATCTACCAGCAAGGCTGCTTTGCTGGTGGGACGGTGCTACGGATTGCCAAGGACGTGGCCGAGAACAATGCCGGAGCTCGTGTTCTGATCGTGTGCTGTGAGATCACCACCATGTTTTTTCAGCAACCTTGTGACAGTCACTTGGATGTTTTGGTCGGACAGGCTCTGTTTTCGGACGGTGCGGCGGCTTTGATTGTTGGGGCCAATCCGGACCCCAAAAGTGAACGTCAACTGTTCAATATCATGTCTGTTAGAGGGACTATTATCCCAAACTCGGAGCATGGAGTTGTGGCACATTTGCGTGAGATGGGGTTTGAGTATTACCTATCATCAGAAGTTCCAAAGTTGGTTGGTGGGAAAATCGAAGAGTGTTTGAGTAAAGGGTTTGAGGGCATTGGGGTTAATGGTGATTGGAACTCGTTGTTCTTTAGCGTTCATCCTGGGGGTCCTGCAATTCTGGACAAGGTTGAAGAAGAGCTGGGTTTGAAGGAGGGGAAGCTGAAGGCAACAAGGCATGTGCTGAGTGAGTTTGGGAATATGGGAGCTCCATCTGTGCTTTTTATTTTGGATGAGATAAGGAAGAAGTCAATGGAGGAAGCAAAAGCCACAACTGGTGAAGGTTTGGAATGGGGTGTGTTAATTGGGATCGGGCCAGGACTCACTGTGGAGACTGTTGTGCTCCGCAGTGCTCCCACCGCTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00941 ko01100 ko01110 ko04712 map00941 map01100 map01110 map04712
Pfam Domains
Protein Families
Chal_sti_synt_C Chal_sti_synt_N

Protein Analysis

387

Amino Acids

42.22

Weight (kDa)

5.98

Isoelectric Point (pI)

36.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chal_sti_synt_N PF00195 3 - 223 1e-100 Chalcone and stilbene synthases, N-terminal domain
Chal_sti_synt_C PF02797 236 - 383 8.9e-52 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000409)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13930
fragaria_vesca FvH4_2g28040 FvH4_2g28050 FvH4_7g01160 FvH4_7g01160 FvH4_7g01160 FvH4_7g01160 FvH4_7g01160 FvH4_7g01160 FvH4_7g01160
malus_domestica MD04G1003000.v1.1 MD04G1003300.v1.1 MD04G1003400.v1.1 MD04G1111500.v1.1 MD08G1116200.v1.1 MD13G1285100.v1.1 MD14G1160800.v1.1 MD14G1160900.v1.1 MD15G1131700.v1.1 MD15G1132000.v1.1 MD15G1132100.v1.1 MD15G1132200.v1.1 MD15G1132300.v1.1
prunus_persica Prupe.1G002900_v2.0.a1 Prupe.1G002900_v2.0.a1 Prupe.1G003000_v2.0.a1 Prupe.4G252100_v2.0.a1 Prupe.4G252800_v2.0.a1 Prupe.4G253000_v2.0.a1 Prupe.4G253100_v2.0.a1 Prupe.I005700_v2.0.a1 Prupe.I005700_v2.0.a1 Prupe.I005800_v2.0.a1
pyrus_communis pycom04g00310 pycom04g00320 pycom04g00350 pycom13g10200 pycom15g11900 pycom15g11910 pycom15g11920 pycom15g11930 pycom15g11940 pycom420g00730
rosa_chinensis RchiOBHm_Chr1g0316441 RchiOBHm_Chr1g0316451 RchiOBHm_Chr1g0316461 RchiOBHm_Chr4g0399981 RchiOBHm_Chr6g0296951 RchiOBHm_Chr6g0297001 RchiOBHm_Chr6g0297011
rosa_laevigata RLG00000009221 RLG00000011646 RLG00000030671 RLG00000030673
rosa_multiflora Rmu_co8031452.1_g000001 Rmu_co8152518.1_g000001 Rmu_sc0000081.1_g000010 Rmu_sc0004672.1_g000001 Rmu_sc0006453.1_g000028 Rmu_sc0006790.1_g000004 Rmu_sc0007272.1_g000004 Rmu_sc0023107.1_g000002 Rmu_sc0023714.1_g000001
rosa_roxburghii Rroxscaffold_4G00330980 Rroxscaffold_4G00330990 Rroxscaffold_4G00331000 Rroxscaffold_7G00171030
rosa_rugosa Rorug01G0010500 Rorug01G0010600 Rorug01G0011000 Rorug03G0299400 Rorug03G0299500 Rorug04G0022100 Rorug05G0381700 Rorug06G0267700
rosa_samantha Rh1AG020500 Rh1BG015600 Rh1BG015800 Rh1CG018400 Rh1CG018600 Rh1CG018800 Rh1DG015500 Rh1DG015700 Rh1DG015900 Rh4AG097700 Rh4BG095700 Rh4CG106900 Rh4DG092500 Rh6AG378300 Rh6BG386300 Rh6CG392100 Rh6CG392300 Rh6DG379000
rosa_wichuraiana Rw0G010230 Rw1G001330 Rw1G001340 Rw4G008110 Rw4G013630 Rw6G033070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 401
AccI GTMKAC 1 cut(s) 390
AccIII TCCGGA 1 cut(s) 667
AciI CCGC 5 cut(s) 311, 641, 644, 1141, 1155
AclWI GGATC 1 cut(s) 1112
AcoI YGGCCR 1 cut(s) 516
AcuI CTGAAG 1 cut(s) 980
AfiI CCNNNNNNNGG 2 cut(s) 299, 733
AgeI ACCGGT 1 cut(s) 450
AgsI TTSAA 7 cut(s) 109, 154, 319, 351, 697, 929, 946
AjiI CACGTC 2 cut(s) 266, 514
AjnI CCWGG 2 cut(s) 898, 1111
AloI GAACNNNNNNTCC 2 cut(s) 525, 557
AluBI AGCT 9 cut(s) 72, 240, 380, 415, 424, 536, 937, 958, 1004
AluI AGCT 9 cut(s) 72, 240, 380, 415, 424, 536, 937, 958, 1004
Alw21I GWGCWC 5 cut(s) 129, 538, 1006, 1140, 1150
Alw26I GTCTC 2 cut(s) 12, 1121
AlwI GGATC 1 cut(s) 1112
AlwNI CAGNNNCTG 1 cut(s) 625
Aor13HI TCCGGA 1 cut(s) 667
AoxI GGCC 6 cut(s) 321, 337, 430, 516, 660, 1109
ApeKI GCWGC 2 cut(s) 237, 474
AsiGI ACCGGT 1 cut(s) 450
AspS9I GGNCC 6 cut(s) 289, 337, 660, 670, 905, 1109
AsuC2I CCSGG 1 cut(s) 399
AsuHPI GGTGA 5 cut(s) 106, 349, 556, 878, 1085
AvaII GGWCC 3 cut(s) 289, 670, 905
BanI GGYRCC 1 cut(s) 401
BanII GRGCYC 2 cut(s) 538, 1006
BauI CACGAG 2 cut(s) 284, 537
BbsI GAAGAC 1 cut(s) 93
Bbv12I GWGCWC 5 cut(s) 129, 538, 1006, 1140, 1150
BbvI GCAGC 2 cut(s) 249, 461
BccI CCATC 5 cut(s) 169, 332, 448, 760, 1015
BcgI CGANNNNNNTGC 2 cut(s) 509, 543
BciT130I CCWGG 2 cut(s) 900, 1113
BcnI CCSGG 1 cut(s) 399
BcoDI GTCTC 2 cut(s) 12, 1121
BfaI CTAG 1 cut(s) 44
BisI GCNGC 5 cut(s) 238, 312, 475, 642, 645
BlsI GCNGC 5 cut(s) 239, 313, 476, 643, 646
Bme1390I CCNGG 3 cut(s) 399, 900, 1113
Bme18I GGWCC 3 cut(s) 289, 670, 905
BmgBI CACGTC 2 cut(s) 266, 514
BmgT120I GGNCC 6 cut(s) 289, 337, 660, 670, 905, 1109
BmiI GGNNCC 8 cut(s) 30, 249, 291, 338, 403, 661, 672, 906
BmrFI CCNGG 3 cut(s) 399, 900, 1113
BoxI GACNNNNGTC 1 cut(s) 264
BpiI GAAGAC 1 cut(s) 93
BpuMI CCSGG 1 cut(s) 399
BsaJI CCNNGG 3 cut(s) 427, 507, 899
BsaWI WCCGGW 2 cut(s) 450, 667
BsaXI ACNNNNNCTCC 2 cut(s) 525, 555
Bsc4I CCNNNNNNNGG 2 cut(s) 299, 733
Bse118I RCCGGY 1 cut(s) 450
Bse1I ACTGG 2 cut(s) 65, 1075
BseAI TCCGGA 1 cut(s) 667
BseBI CCWGG 2 cut(s) 900, 1113
BseDI CCNNGG 3 cut(s) 427, 507, 899
BseGI GGATG 3 cut(s) 610, 895, 1033
BseLI CCNNNNNNNGG 2 cut(s) 299, 733
BseMII CTCAG 2 cut(s) 23, 971
BseNI ACTGG 2 cut(s) 65, 1075
BseRI GAGGAG 1 cut(s) 416
BseXI GCAGC 2 cut(s) 249, 461
BseYI CCCAGC 1 cut(s) 937
BsgI GTGCAG 1 cut(s) 358
BshFI GGCC 6 cut(s) 323, 339, 432, 518, 662, 1111
BshNI GGYRCC 1 cut(s) 401
BshTI ACCGGT 1 cut(s) 450
BsiHKAI GWGCWC 5 cut(s) 129, 538, 1006, 1140, 1150
BsiSI CCGG 4 cut(s) 399, 451, 531, 668
BslFI GGGAC 4 cut(s) 67, 275, 502, 730
BslI CCNNNNNNNGG 2 cut(s) 299, 733
BsmAI GTCTC 2 cut(s) 12, 1121
BsmFI GGGAC 4 cut(s) 67, 275, 502, 730
BsnI GGCC 6 cut(s) 323, 339, 432, 518, 662, 1111
Bsp1286I GDGCHC 5 cut(s) 129, 538, 1006, 1140, 1150
Bsp13I TCCGGA 1 cut(s) 667
Bsp143I GATC 4 cut(s) 459, 546, 561, 1104
BspACI CCGC 5 cut(s) 311, 641, 644, 1141, 1155
BspANI GGCC 6 cut(s) 323, 339, 432, 518, 662, 1111
BspCNI CTCAG 2 cut(s) 22, 972
BspEI TCCGGA 1 cut(s) 667
BspHI TCATGA 1 cut(s) 456
BspLI GGNNCC 8 cut(s) 30, 249, 291, 338, 403, 661, 672, 906
BspPI GGATC 1 cut(s) 1112
BspQI GCTCTTC 1 cut(s) 927
BspT107I GGYRCC 1 cut(s) 401
BsrFI RCCGGY 1 cut(s) 450
BsrI ACTGG 2 cut(s) 65, 1075
BssAI RCCGGY 1 cut(s) 450
BssECI CCNNGG 3 cut(s) 427, 507, 899
BssMI GATC 4 cut(s) 459, 546, 561, 1104
BssSI CACGAG 2 cut(s) 284, 537
BssT1I CCWWGG 1 cut(s) 507
Bst2BI CACGAG 2 cut(s) 284, 537
Bst2UI CCWGG 2 cut(s) 900, 1113
Bst4CI ACNGT 6 cut(s) 493, 596, 638, 693, 1123, 1132
Bst6I CTCTTC 3 cut(s) 201, 819, 927
BstC8I GCNNGC 1 cut(s) 125
BstDEI CTNAG 2 cut(s) 9, 980
BstF5I GGATG 3 cut(s) 610, 895, 1033
BstKTI GATC 4 cut(s) 462, 549, 564, 1107
BstMAI GTCTC 2 cut(s) 12, 1121
BstMBI GATC 4 cut(s) 459, 546, 561, 1104
BstMWI GCNNNNNNNGC 4 cut(s) 20, 320, 421, 1154
BstNI CCWGG 2 cut(s) 900, 1113
BstNSI RCATGY 2 cut(s) 397, 977
BstPAI GACNNNNGTC 1 cut(s) 264
BstSCI CCNGG 3 cut(s) 397, 898, 1111
BstV1I GCAGC 2 cut(s) 249, 461
BstV2I GAAGAC 1 cut(s) 93
BstXI CCANNNNNNTGG 1 cut(s) 514
BsuRI GGCC 6 cut(s) 323, 339, 432, 518, 662, 1111
BtrI CACGTC 2 cut(s) 266, 514
BtsCI GGATG 3 cut(s) 610, 895, 1033
BtsI GCAGTG 1 cut(s) 1150
BtsIMutI CAGTG 2 cut(s) 1119, 1150
Cac8I GCNNGC 1 cut(s) 125
CaiI CAGNNNCTG 1 cut(s) 625
CciI TCATGA 1 cut(s) 456
Cfr10I RCCGGY 1 cut(s) 450
Cfr13I GGNCC 6 cut(s) 289, 337, 660, 670, 905, 1109
CseI GACGC 2 cut(s) 269, 376
CspAI ACCGGT 1 cut(s) 450
CviAII CATG 6 cut(s) 394, 457, 571, 703, 740, 974
DdeI CTNAG 2 cut(s) 9, 980
DpnI GATC 4 cut(s) 461, 548, 563, 1106
DpnII GATC 4 cut(s) 459, 546, 561, 1104
EaeI YGGCCR 1 cut(s) 516
Eam1104I CTCTTC 3 cut(s) 201, 819, 927
EarI CTCTTC 3 cut(s) 201, 819, 927
Ecl136II GAGCTC 2 cut(s) 536, 1004
Eco130I CCWWGG 1 cut(s) 507
Eco24I GRGCYC 2 cut(s) 538, 1006
Eco32I GATATC 1 cut(s) 191
Eco47I GGWCC 3 cut(s) 289, 670, 905
Eco53kI GAGCTC 2 cut(s) 536, 1004
Eco57I CTGAAG 1 cut(s) 980
EcoICRI GAGCTC 2 cut(s) 536, 1004
EcoO109I RGGNCCY 2 cut(s) 289, 905
EcoRII CCWGG 2 cut(s) 898, 1111
EcoRV GATATC 1 cut(s) 191
EcoT14I CCWWGG 1 cut(s) 507
EcoT38I GRGCYC 2 cut(s) 538, 1006
ErhI CCWWGG 1 cut(s) 507
FaeI CATG 6 cut(s) 397, 460, 574, 706, 743, 977
FaqI GGGAC 4 cut(s) 67, 275, 502, 730
FatI CATG 6 cut(s) 393, 456, 570, 702, 739, 973
FauNDI CATATG 2 cut(s) 161, 233
FblI GTMKAC 1 cut(s) 390
Fnu4HI GCNGC 5 cut(s) 238, 312, 475, 642, 645
FokI GGATG 3 cut(s) 617, 882, 1040
FriOI GRGCYC 2 cut(s) 538, 1006
Fsp4HI GCNGC 5 cut(s) 238, 312, 475, 642, 645
FspBI CTAG 1 cut(s) 44
GluI GCNGC 5 cut(s) 238, 312, 475, 642, 645
GsaI CCCAGC 1 cut(s) 941
HaeIII GGCC 6 cut(s) 323, 339, 432, 518, 662, 1111
HapII CCGG 4 cut(s) 399, 451, 531, 668
HgaI GACGC 2 cut(s) 269, 376
Hin1II CATG 6 cut(s) 397, 460, 574, 706, 743, 977
HincII GTYRAC 2 cut(s) 391, 689
HindII GTYRAC 2 cut(s) 391, 689
HinfI GANTC 5 cut(s) 5, 198, 211, 280, 1116
HpaII CCGG 4 cut(s) 399, 451, 531, 668
HphI GGTGA 5 cut(s) 106, 349, 556, 878, 1085
Hpy166II GTNNAC 3 cut(s) 391, 683, 689
Hpy188I TCNGA 6 cut(s) 329, 546, 617, 634, 736, 793
Hpy188III TCNNGA 6 cut(s) 95, 269, 284, 457, 668, 917
Hpy8I GTNNAC 3 cut(s) 391, 683, 689
Hpy99I CGWCG 2 cut(s) 263, 392
HpyAV CCTTC 4 cut(s) 313, 940, 955, 1070
HpyCH4III ACNGT 6 cut(s) 493, 596, 638, 693, 1123, 1132
HpyCH4IV ACGT 3 cut(s) 265, 513, 685
HpyCH4V TGCA 4 cut(s) 59, 237, 375, 911
HpyF10VI GCNNNNNNNGC 4 cut(s) 20, 320, 421, 1154
HpyF3I CTNAG 2 cut(s) 9, 980
HpySE526I ACGT 3 cut(s) 265, 513, 685
Hsp92II CATG 6 cut(s) 397, 460, 574, 706, 743, 977
Kpn2I TCCGGA 1 cut(s) 667
Kzo9I GATC 4 cut(s) 459, 546, 561, 1104
LguI GCTCTTC 1 cut(s) 927
LmnI GCTCC 8 cut(s) 253, 429, 533, 736, 1001, 1009, 1143, 1153
Lsp1109I GCAGC 2 cut(s) 249, 461
MaeI CTAG 1 cut(s) 44
MaeII ACGT 3 cut(s) 265, 513, 685
MaeIII GTNAC 5 cut(s) 74, 112, 199, 590, 596
MalI GATC 4 cut(s) 461, 548, 563, 1106
MboI GATC 4 cut(s) 459, 546, 561, 1104
MboII GAAGA 7 cut(s) 98, 218, 361, 836, 941, 944, 1051
MhlI GDGCHC 5 cut(s) 129, 538, 1006, 1140, 1150
MluCI AATT 3 cut(s) 137, 912, 1098
MlyI GAGTC 3 cut(s) 14, 207, 1110
MmeI TCCRAC 1 cut(s) 595
MnlI CCTC 9 cut(s) 262, 280, 365, 374, 437, 707, 841, 943, 1045
MroI TCCGGA 1 cut(s) 667
MseI TTAA 3 cut(s) 140, 861, 1097
MslI CAYNNNNRTG 3 cut(s) 569, 603, 759
MspI CCGG 4 cut(s) 399, 451, 531, 668
MspR9I CCNGG 3 cut(s) 399, 900, 1113
MvaI CCWGG 2 cut(s) 900, 1113
MwoI GCNNNNNNNGC 4 cut(s) 20, 320, 421, 1154
NciI CCSGG 1 cut(s) 399
NdeI CATATG 2 cut(s) 161, 233
NdeII GATC 4 cut(s) 459, 546, 561, 1104
NlaIII CATG 6 cut(s) 397, 460, 574, 706, 743, 977
NlaIV GGNNCC 8 cut(s) 30, 249, 291, 338, 403, 661, 672, 906
NmeAIII GCCGAG 2 cut(s) 408, 544
NmuCI GTSAC 4 cut(s) 112, 199, 590, 596
NspI RCATGY 2 cut(s) 397, 977
PagI TCATGA 1 cut(s) 456
PciSI GCTCTTC 1 cut(s) 927
PfeI GAWTC 2 cut(s) 211, 280
PinAI ACCGGT 1 cut(s) 450
PkrI GCNGC 5 cut(s) 239, 313, 476, 643, 646
PleI GAGTC 3 cut(s) 13, 206, 1110
PpsI GAGTC 3 cut(s) 13, 206, 1110
PpuMI RGGWCCY 2 cut(s) 289, 905
PshAI GACNNNNGTC 1 cut(s) 264
Psp124BI GAGCTC 2 cut(s) 538, 1006
Psp5II RGGWCCY 2 cut(s) 289, 905
Psp6I CCWGG 2 cut(s) 898, 1111
PspFI CCCAGC 1 cut(s) 937
PspGI CCWGG 2 cut(s) 898, 1111
PspN4I GGNNCC 8 cut(s) 30, 249, 291, 338, 403, 661, 672, 906
PspPI GGNCC 6 cut(s) 289, 337, 660, 670, 905, 1109
PspPPI RGGWCCY 2 cut(s) 289, 905
PstNI CAGNNNCTG 1 cut(s) 625
RseI CAYNNNNRTG 3 cut(s) 569, 603, 759
SacI GAGCTC 2 cut(s) 538, 1006
SalI GTCGAC 1 cut(s) 389
SapI GCTCTTC 1 cut(s) 927
SaqAI TTAA 3 cut(s) 140, 861, 1097
SatI GCNGC 5 cut(s) 238, 312, 475, 642, 645
Sau3AI GATC 4 cut(s) 459, 546, 561, 1104
Sau96I GGNCC 6 cut(s) 289, 337, 660, 670, 905, 1109
SchI GAGTC 3 cut(s) 14, 207, 1110
ScrFI CCNGG 3 cut(s) 399, 900, 1113
SduI GDGCHC 5 cut(s) 129, 538, 1006, 1140, 1150
SinI GGWCC 3 cut(s) 289, 670, 905
SmiMI CAYNNNNRTG 3 cut(s) 569, 603, 759
Sse9I AATT 3 cut(s) 137, 912, 1098
SsiI CCGC 5 cut(s) 311, 641, 644, 1141, 1155
SspMI CTAG 1 cut(s) 44
SstI GAGCTC 2 cut(s) 538, 1006
StyD4I CCNGG 3 cut(s) 397, 898, 1111
StyI CCWWGG 1 cut(s) 507
TaaI ACNGT 6 cut(s) 493, 596, 638, 693, 1123, 1132
TaiI ACGT 3 cut(s) 268, 516, 688
TaqI TCGA 4 cut(s) 173, 258, 390, 822
TaqII GACCGA 1 cut(s) 149
TasI AATT 3 cut(s) 137, 912, 1098
TauI GCSGC 3 cut(s) 314, 644, 647
TfiI GAWTC 2 cut(s) 211, 280
Tru1I TTAA 3 cut(s) 140, 861, 1097
Tru9I TTAA 3 cut(s) 140, 861, 1097
TscAI CASTG 2 cut(s) 1126, 1150
TseFI GTSAC 4 cut(s) 112, 199, 590, 596
TseI GCWGC 2 cut(s) 237, 474
Tsp45I GTSAC 4 cut(s) 112, 199, 590, 596
TspDTI ATGAA 2 cut(s) 445, 884
TspGWI ACGGA 1 cut(s) 514
TspRI CASTG 2 cut(s) 1126, 1150
VpaK11BI GGWCC 3 cut(s) 289, 670, 905
XceI RCATGY 2 cut(s) 397, 977
XmiI GTMKAC 1 cut(s) 390
XspI CTAG 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.