Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0000375

Physical Location & Seq

Genomic Coordinates Reverse (-)

27890331 .. 27891701

Gene Structure 1371 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1188300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1371 bp

ATGGGGCACGTTCGCTACATCTCCACAACCACTGTTCAACCCACACTCCAGAACGAGTCCACTCAGAGAATCGAGTTAACTCCATGGGATTTGCAGTTCATCCTATTCGGCTACAGCCAAAAGGGCCTTCTCTTCCACAAACCAAAACCCAGTACTTATGACAAGTACCCAAGCAAAAGCTTGATCCAACACCTACAAACCTCCCTCTCACTCACGTTGGATATCTTCTATCCCCTTGCTGGCCGTCTTGCCATCACCGAAAACGAAGACGACAACACTACCTCCTTCTCCGTCGATTGTAATGGCGCTGGAGCTCAATTTGTCCATGCAGTTGCTGATGGTGTCACGGTTGCCGATATTCTCGACCCGGTTTTGGTTCCCAGTGACATTGTTCACTCATTCTTTTTTTTGAATGGAGTTTTGAACTATGAAGGCGTGTCGGAACCCTTGCTTGCTGTGCAAGTAACCGAGCTGTTTGATGGCATTTTCATCGGTTGCACCACGAACCACTCTGTCGTTGATGGGTCAACATTTTGGCGTTTCTTCAATACTTGGTCGGAAATCTCTCGGCGTACAGAAACTAGTACTGAAAATTGTCGTAAAATTTCGCAGCCACCTCCCATTTTTAGCCGTGAGTTTTTTGACGGCGGAATCATTGATTTCCCGGTTCGAATTGCAAACTTTCACAATCAAGTCCCAAAAAATAGGTCTCCCGCACTATCCGCCCCCCTTCATCAAAGAATATTTAATTTTTCCAAAGAAAAAATTGCTCAACTCAAAGCCAAAGCCAATGCCGAGATGGGCACCACCAAGATCTCATCCCTTCAAGCACTACTGGCTCATCTTTGGTTATCCATTATCCGCAACAAACATCTCAATCCCGACCAAGAGAGCAAGTATACCTTACTAGTAGGCTTAAGACAAAGATTCCAACTGCCGTTACCTGAAGAATACCTCGGAAATGTGGTTCAATTTGGCACGGTGACATCCACTGTGAGCGATCTGCTCGAGCACGGACTAGGCTGGACGGCTTGGGAGATAAACAAGATGATTGCTTCCAAGACGAAAGACGAGGTGAGAAAGTATTTGGAGGAATGGATAGAGAGTCCACAACTTATCCAACTGAATAATTTGGCAAGTGATTACTCATTTGGGACGGGAAGCTCACCACGGTTCAATATGTACGGTAATGACTTTGGTTGGGGAAGGCCGCTGGCAGTTAGAAGTGGCGCAGCCAACAAGTCCGTGGGAAAGTTGACTGTAGCTCCTGGGGCTGAAGATGGAAGTATTGAGTTTGAAGCTTGTTTGTCACCTCAGACACTGCAAGCTTTGGACGAGGACGCAGAGTTCATTGCCAGTTTGTCCAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002831 GO:0006082 GO:0006725 GO:0008150 GO:0008152 GO:0009058 GO:0009605 GO:0009607 GO:0009617 GO:0009696 GO:0009697 GO:0009719 GO:0009725 GO:0009753 GO:0009987 GO:0010033 GO:0010817 GO:0016053 GO:0016999 GO:0017000 GO:0017144 GO:0018958 GO:0019438 GO:0019752 GO:0031347 GO:0032101 GO:0032787 GO:0042221 GO:0042445 GO:0042446 GO:0042537 GO:0043207 GO:0043436 GO:0043900 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0046189 GO:0046394 GO:0048583 GO:0050789 GO:0050896 GO:0051704 GO:0051707 GO:0065007 GO:0065008 GO:0071704 GO:0072330 GO:0080134 GO:1900150 GO:1900424 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Transferase

Protein Analysis

457

Amino Acids

50.85

Weight (kDa)

5.61

Isoelectric Point (pI)

42.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Transferase	PF02458	3 - 450	1.8e-77	Transferase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000375)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G50270 AT3G50280 AT3G50280 AT3G50290 AT3G50300 AT5G38130 AT5G67150
fragaria_vesca	FvH4_1g16271 FvH4_1g16300 FvH4_1g16301 FvH4_1g16304 FvH4_1g16321
malus_domestica	MD02G1175300.v1.1 MD02G1175400.v1.1 MD02G1175500.v1.1 MD02G1175700.v1.1 MD03G1116500.v1.1 MD04G1188300.v1.1 MD08G1137000.v1.1 MD15G1286000.v1.1 MD15G1286100.v1.1 MD15G1286200.v1.1 MD17G1197100.v1.1
prunus_persica	Prupe.7G129900_v2.0.a1 Prupe.7G130000_v2.0.a1 Prupe.7G130100_v2.0.a1 Prupe.7G130200_v2.0.a1 Prupe.7G130300_v2.0.a1 Prupe.7G130400_v2.0.a1 Prupe.7G130500_v2.0.a1 Prupe.7G130500_v2.0.a1 Prupe.7G130600_v2.0.a1 Prupe.7G130700_v2.0.a1 Prupe.7G130800_v2.0.a1
pyrus_communis	pycom02g14140 pycom02g14150 pycom15g24910 pycom17g20220
rosa_chinensis	RchiOBHm_Chr2g0105981 RchiOBHm_Chr2g0106021 RchiOBHm_Chr2g0106071 RchiOBHm_Chr2g0106091 RchiOBHm_Chr2g0106111 RchiOBHm_Chr2g0106181 RchiOBHm_Chr2g0106441
rosa_laevigata	RLG00000000204 RLG00000000206 RLG00000000207 RLG00000000208 RLG00000017475 RLG00000017476 RLG00000017477 RLG00000017479 RLG00000017498
rosa_multiflora	Rmu_co8282453.1_g000001 Rmu_sc0000444.1_g000002 Rmu_sc0000608.1_g000005 Rmu_sc0004015.1_g000005 Rmu_sc0008303.1_g000013 Rmu_sc0009027.1_g000012 Rmu_sc0010662.1_g000004 Rmu_sc0014348.1_g000011
rosa_roxburghii	Rroxscaffold_2G00137180 Rroxscaffold_2G00137400 Rroxscaffold_2G00137420 Rroxscaffold_2G00137440 Rroxscaffold_2G00137460 Rroxscaffold_2G00137490 Rroxscaffold_2G00137610 Rroxscaffold_2G00137630 Rroxscaffold_2G00137650
rosa_rugosa	Rorug02G0132900 Rorug02G0133000 Rorug02G0133100 Rorug02G0133200 Rorug02G0135400
rosa_samantha	Rh2AG183800 Rh2AG184000 Rh2AG184100 Rh2AG184200 Rh2AG186700 Rh2AG186800 Rh2BG193500 Rh2BG193700 Rh2BG193900 Rh2BG194000 Rh2BG194200 Rh2BG197000 Rh2BG197200 Rh2CG187700 Rh2CG188100 Rh2CG188500 Rh2CG188600 Rh2CG188700 Rh2CG189100 Rh2CG191800 Rh2DG190200 Rh2DG190400 Rh2DG190500 Rh2DG190600 Rh2DG192800 Rh2DG193000
rosa_wichuraiana	Rw2G014370 Rw2G014550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	803
AccI	GTMKAC	1 cut(s)	899
AciI	CCGC	5 cut(s)	648, 714, 723, 862, 1211
AclWI	GGATC	1 cut(s)	178
AcoI	YGGCCR	1 cut(s)	241
AcsI	RAATTY	1 cut(s)	603
AcuI	CTGAAG	2 cut(s)	966, 1296
AfaI	GTAC	5 cut(s)	154, 167, 574, 586, 1184
AfiI	CCNNNNNNNGG	2 cut(s)	239, 373
AflII	CTTAAG	1 cut(s)	916
AgsI	TTSAA	8 cut(s)	38, 412, 424, 547, 827, 971, 1177, 1298
AhlI	ACTAGT	2 cut(s)	581, 907
AjnI	CCWGG	1 cut(s)	1267
AjuI	GAANNNNNNNTTGG	2 cut(s)	111, 143
AloI	GAACNNNNNNTCC	6 cut(s)	80, 112, 951, 983, 1331, 1363
AluBI	AGCT	7 cut(s)	180, 314, 472, 1164, 1265, 1301, 1328
AluI	AGCT	7 cut(s)	180, 314, 472, 1164, 1265, 1301, 1328
Alw21I	GWGCWC	2 cut(s)	316, 1014
Alw26I	GTCTC	1 cut(s)	714
AlwI	GGATC	1 cut(s)	178
AlwNI	CAGNNNCTG	2 cut(s)	335, 1321
Ama87I	CYCGRG	1 cut(s)	1007
AoxI	GGCC	3 cut(s)	124, 241, 1208
ApeKI	GCWGC	2 cut(s)	610, 1232
ApoI	RAATTY	1 cut(s)	603
ArsI	GACNNNNNNTTYG	2 cut(s)	692, 724
AspLEI	GCGC	2 cut(s)	308, 1232
AspS9I	GGNCC	1 cut(s)	124
AsuC2I	CCSGG	2 cut(s)	368, 665
AsuHPI	GGTGA	5 cut(s)	247, 994, 1087, 1158, 1302
AsuII	TTCGAA	1 cut(s)	670
AvaI	CYCGRG	1 cut(s)	1007
BaeGI	GKGCMC	2 cut(s)	9, 806
BanI	GGYRCC	1 cut(s)	803
BanII	GRGCYC	1 cut(s)	316
BbsI	GAAGAC	1 cut(s)	273
Bbv12I	GWGCWC	2 cut(s)	316, 1014
BbvI	GCAGC	2 cut(s)	622, 1244
BccI	CCATC	6 cut(s)	260, 332, 473, 515, 793, 1274
BceAI	ACGGC	5 cut(s)	228, 615, 661, 922, 1044
BcgI	CGANNNNNNTGC	2 cut(s)	472, 506
BciT130I	CCWGG	1 cut(s)	1269
BcnI	CCSGG	2 cut(s)	368, 665
BcoDI	GTCTC	1 cut(s)	714
BcuI	ACTAGT	2 cut(s)	581, 907
BfaI	CTAG	3 cut(s)	582, 908, 1019
BfmI	CTRYAG	2 cut(s)	112, 1260
BfoI	RGCGCY	1 cut(s)	309
BfrI	CTTAAG	1 cut(s)	916
BglI	GCCNNNNNGGC	1 cut(s)	123
BglII	AGATCT	1 cut(s)	813
BisI	GCNGC	3 cut(s)	611, 1211, 1233
BlsI	GCNGC	3 cut(s)	612, 1212, 1234
BmcAI	AGTACT	2 cut(s)	154, 586
Bme1390I	CCNGG	3 cut(s)	368, 665, 1269
BmeT110I	CYCGRG	1 cut(s)	1007
BmgT120I	GGNCC	1 cut(s)	124
BmiI	GGNNCC	3 cut(s)	378, 444, 805
BmrFI	CCNGG	3 cut(s)	368, 665, 1269
BmrI	ACTGGG	2 cut(s)	144, 375
BmuI	ACTGGG	2 cut(s)	144, 375
BpiI	GAAGAC	1 cut(s)	273
BpmI	CTGGAG	2 cut(s)	32, 330
Bpu14I	TTCGAA	1 cut(s)	670
BpuMI	CCSGG	2 cut(s)	368, 665
BsaI	GGTCTC	1 cut(s)	714
BsaJI	CCNNGG	5 cut(s)	83, 955, 1169, 1245, 1268
BsaXI	ACNNNNNCTCC	6 cut(s)	30, 60, 266, 296, 1249, 1279
Bsc4I	CCNNNNNNNGG	2 cut(s)	239, 373
Bse1I	ACTGG	4 cut(s)	150, 381, 840, 1356
Bse3DI	GCAATG	1 cut(s)	1350
BseBI	CCWGG	1 cut(s)	1269
BseDI	CCNNGG	5 cut(s)	83, 955, 1169, 1245, 1268
BseGI	GGATG	3 cut(s)	99, 818, 986
BseLI	CCNNNNNNNGG	2 cut(s)	239, 373
BseMI	GCAATG	1 cut(s)	1350
BseMII	CTCAG	2 cut(s)	77, 1328
BseNI	ACTGG	4 cut(s)	150, 381, 840, 1356
BseSI	GKGCMC	2 cut(s)	9, 806
BseXI	GCAGC	2 cut(s)	622, 1244
BshFI	GGCC	3 cut(s)	126, 243, 1210
BshNI	GGYRCC	1 cut(s)	803
BsiHKAI	GWGCWC	2 cut(s)	316, 1014
BsiHKCI	CYCGRG	1 cut(s)	1007
BsiSI	CCGG	2 cut(s)	368, 665
BslFI	GGGAC	2 cut(s)	680, 1168
BslI	CCNNNNNNNGG	2 cut(s)	239, 373
BsmAI	GTCTC	1 cut(s)	714
BsmFI	GGGAC	2 cut(s)	680, 1168
BsnI	GGCC	3 cut(s)	126, 243, 1210
Bso31I	GGTCTC	1 cut(s)	714
BsoBI	CYCGRG	1 cut(s)	1007
Bsp119I	TTCGAA	1 cut(s)	670
Bsp1286I	GDGCHC	4 cut(s)	9, 316, 806, 1014
Bsp143I	GATC	3 cut(s)	183, 813, 1000
Bsp19I	CCATGG	1 cut(s)	83
BspACI	CCGC	5 cut(s)	648, 714, 723, 862, 1211
BspANI	GGCC	3 cut(s)	126, 243, 1210
BspCNI	CTCAG	2 cut(s)	76, 1327
BspLI	GGNNCC	3 cut(s)	378, 444, 805
BspPI	GGATC	1 cut(s)	178
BspT104I	TTCGAA	1 cut(s)	670
BspT107I	GGYRCC	1 cut(s)	803
BspTI	CTTAAG	1 cut(s)	916
BspTNI	GGTCTC	1 cut(s)	714
BsrDI	GCAATG	1 cut(s)	1350
BsrI	ACTGG	4 cut(s)	150, 381, 840, 1356
BssECI	CCNNGG	5 cut(s)	83, 955, 1169, 1245, 1268
BssMI	GATC	3 cut(s)	183, 813, 1000
BssNAI	GTATAC	1 cut(s)	900
BssT1I	CCWWGG	1 cut(s)	83
Bst1107I	GTATAC	1 cut(s)	900
Bst2UI	CCWGG	1 cut(s)	1269
Bst4CI	ACNGT	7 cut(s)	34, 349, 982, 994, 1173, 1187, 1261
Bst6I	CTCTTC	1 cut(s)	137
BstAFI	CTTAAG	1 cut(s)	916
BstBI	TTCGAA	1 cut(s)	670
BstC8I	GCNNGC	4 cut(s)	241, 453, 1215, 1326
BstDEI	CTNAG	2 cut(s)	63, 1314
BstDSI	CCRYGG	3 cut(s)	83, 1169, 1245
BstF5I	GGATG	3 cut(s)	99, 818, 986
BstH2I	RGCGCY	1 cut(s)	309
BstHHI	GCGC	2 cut(s)	308, 1232
BstKTI	GATC	3 cut(s)	186, 816, 1003
BstMAI	GTCTC	1 cut(s)	714
BstMBI	GATC	3 cut(s)	183, 813, 1000
BstMWI	GCNNNNNNNGC	5 cut(s)	123, 457, 722, 836, 1271
BstNI	CCWGG	1 cut(s)	1269
BstSCI	CCNGG	3 cut(s)	366, 663, 1267
BstSFI	CTRYAG	2 cut(s)	112, 1260
BstSLI	GKGCMC	2 cut(s)	9, 806
BstV1I	GCAGC	2 cut(s)	622, 1244
BstV2I	GAAGAC	1 cut(s)	273
BstX2I	RGATCY	1 cut(s)	813
BstYI	RGATCY	1 cut(s)	813
BstZ17I	GTATAC	1 cut(s)	900
BsuRI	GGCC	3 cut(s)	126, 243, 1210
BtgI	CCRYGG	3 cut(s)	83, 1169, 1245
BtsCI	GGATG	3 cut(s)	99, 818, 986
BtsI	GCAGTG	1 cut(s)	1319
BtsIMutI	CAGTG	4 cut(s)	30, 388, 990, 1319
Cac8I	GCNNGC	4 cut(s)	241, 453, 1215, 1326
CaiI	CAGNNNCTG	2 cut(s)	335, 1321
CfoI	GCGC	2 cut(s)	308, 1232
Cfr13I	GGNCC	1 cut(s)	124
CseI	GACGC	1 cut(s)	1349
Csp6I	GTAC	5 cut(s)	153, 166, 573, 585, 1183
CviAII	CATG	2 cut(s)	84, 326
CviQI	GTAC	5 cut(s)	153, 166, 573, 585, 1183
DdeI	CTNAG	2 cut(s)	63, 1314
DpnI	GATC	3 cut(s)	185, 815, 1002
DpnII	GATC	3 cut(s)	183, 813, 1000
EaeI	YGGCCR	1 cut(s)	241
Eam1104I	CTCTTC	1 cut(s)	137
EarI	CTCTTC	1 cut(s)	137
EciI	GGCGGA	2 cut(s)	663, 712
Ecl136II	GAGCTC	1 cut(s)	314
Eco130I	CCWWGG	1 cut(s)	83
Eco24I	GRGCYC	1 cut(s)	316
Eco31I	GGTCTC	1 cut(s)	714
Eco32I	GATATC	1 cut(s)	223
Eco53kI	GAGCTC	1 cut(s)	314
Eco57I	CTGAAG	2 cut(s)	966, 1296
Eco88I	CYCGRG	1 cut(s)	1007
EcoICRI	GAGCTC	1 cut(s)	314
EcoO109I	RGGNCCY	1 cut(s)	124
EcoRII	CCWGG	1 cut(s)	1267
EcoRV	GATATC	1 cut(s)	223
EcoT14I	CCWWGG	1 cut(s)	83
EcoT38I	GRGCYC	1 cut(s)	316
ErhI	CCWWGG	1 cut(s)	83
FaeI	CATG	2 cut(s)	87, 329
FaiI	YATR	6 cut(s)	85, 159, 327, 429, 900, 1181
FalI	AAGNNNNNCTT	2 cut(s)	887, 919
FaqI	GGGAC	2 cut(s)	680, 1168
FatI	CATG	2 cut(s)	83, 325
FauI	CCCGC	1 cut(s)	721
FblI	GTMKAC	1 cut(s)	899
Fnu4HI	GCNGC	3 cut(s)	611, 1211, 1233
FokI	GGATG	3 cut(s)	86, 805, 973
FriOI	GRGCYC	1 cut(s)	316
Fsp4HI	GCNGC	3 cut(s)	611, 1211, 1233
FspBI	CTAG	3 cut(s)	582, 908, 1019
GlaI	GCGC	2 cut(s)	307, 1231
GluI	GCNGC	3 cut(s)	611, 1211, 1233
GsuI	CTGGAG	2 cut(s)	32, 330
HaeII	RGCGCY	1 cut(s)	309
HaeIII	GGCC	3 cut(s)	126, 243, 1210
HapII	CCGG	2 cut(s)	368, 665
HgaI	GACGC	1 cut(s)	1349
HhaI	GCGC	2 cut(s)	308, 1232
Hin1II	CATG	2 cut(s)	87, 329
Hin6I	GCGC	2 cut(s)	306, 1230
HinP1I	GCGC	2 cut(s)	306, 1230
HincII	GTYRAC	3 cut(s)	78, 528, 1257
HindII	GTYRAC	3 cut(s)	78, 528, 1257
HindIII	AAGCTT	3 cut(s)	178, 1299, 1326
HinfI	GANTC	5 cut(s)	56, 69, 651, 927, 1105
HpaI	GTTAAC	1 cut(s)	78
HpaII	CCGG	2 cut(s)	368, 665
HphI	GGTGA	5 cut(s)	247, 994, 1087, 1158, 1302
Hpy166II	GTNNAC	7 cut(s)	60, 78, 394, 528, 900, 1109, 1257
Hpy188I	TCNGA	5 cut(s)	66, 442, 559, 959, 1317
Hpy188III	TCNNGA	3 cut(s)	49, 362, 881
Hpy8I	GTNNAC	7 cut(s)	60, 78, 394, 528, 900, 1109, 1257
Hpy99I	CGWCG	1 cut(s)	296
HpyAV	CCTTC	6 cut(s)	137, 295, 425, 740, 833, 1200
HpyCH4III	ACNGT	7 cut(s)	34, 349, 982, 994, 1173, 1187, 1261
HpyCH4IV	ACGT	2 cut(s)	9, 215
HpyCH4V	TGCA	6 cut(s)	94, 329, 460, 498, 677, 1324
HpyF10VI	GCNNNNNNNGC	5 cut(s)	123, 457, 722, 836, 1271
HpyF3I	CTNAG	2 cut(s)	63, 1314
HpySE526I	ACGT	2 cut(s)	9, 215
Hsp92II	CATG	2 cut(s)	87, 329
HspAI	GCGC	2 cut(s)	306, 1230
KspAI	GTTAAC	1 cut(s)	78
Kzo9I	GATC	3 cut(s)	183, 813, 1000
LmnI	GCTCC	2 cut(s)	311, 1270
Lsp1109I	GCAGC	2 cut(s)	622, 1244
MaeI	CTAG	3 cut(s)	582, 908, 1019
MaeII	ACGT	2 cut(s)	9, 215
MaeIII	GTNAC	6 cut(s)	343, 383, 463, 939, 982, 1308
MalI	GATC	3 cut(s)	185, 815, 1002
MboI	GATC	3 cut(s)	183, 813, 1000
MboII	GAAGA	6 cut(s)	124, 217, 278, 535, 959, 1289
MflI	RGATCY	1 cut(s)	813
MhlI	GDGCHC	4 cut(s)	9, 316, 806, 1014
MluCI	AATT	8 cut(s)	317, 592, 603, 672, 748, 765, 971, 1129
MlyI	GAGTC	2 cut(s)	65, 1114
MmeI	TCCRAC	6 cut(s)	198, 211, 420, 537, 955, 1144
MnlI	CCTC	9 cut(s)	211, 215, 292, 627, 965, 1066, 1084, 1323, 1330
MseI	TTAA	3 cut(s)	77, 747, 917
MspA1I	CMGCKG	1 cut(s)	1213
MspCI	CTTAAG	1 cut(s)	916
MspI	CCGG	2 cut(s)	368, 665
MspR9I	CCNGG	3 cut(s)	368, 665, 1269
MvaI	CCWGG	1 cut(s)	1269
MwoI	GCNNNNNNNGC	5 cut(s)	123, 457, 722, 836, 1271
NciI	CCSGG	2 cut(s)	368, 665
NcoI	CCATGG	1 cut(s)	83
NdeII	GATC	3 cut(s)	183, 813, 1000
NlaIII	CATG	2 cut(s)	87, 329
NlaIV	GGNNCC	3 cut(s)	378, 444, 805
NmeAIII	GCCGAG	2 cut(s)	547, 820
NmuCI	GTSAC	4 cut(s)	343, 383, 982, 1308
NspV	TTCGAA	1 cut(s)	670
PaeR7I	CTCGAG	1 cut(s)	1007
PfeI	GAWTC	3 cut(s)	69, 651, 927
PkrI	GCNGC	3 cut(s)	612, 1212, 1234
PleI	GAGTC	2 cut(s)	64, 1113
PpsI	GAGTC	2 cut(s)	64, 1113
Psp124BI	GAGCTC	1 cut(s)	316
Psp6I	CCWGG	1 cut(s)	1267
PspGI	CCWGG	1 cut(s)	1267
PspN4I	GGNNCC	3 cut(s)	378, 444, 805
PspPI	GGNCC	1 cut(s)	124
PspXI	VCTCGAGB	1 cut(s)	1007
PstNI	CAGNNNCTG	2 cut(s)	335, 1321
PsuI	RGATCY	1 cut(s)	813
RsaI	GTAC	5 cut(s)	154, 167, 574, 586, 1184
RsaNI	GTAC	5 cut(s)	153, 166, 573, 585, 1183
SacI	GAGCTC	1 cut(s)	316
SaqAI	TTAA	3 cut(s)	77, 747, 917
SatI	GCNGC	3 cut(s)	611, 1211, 1233
Sau3AI	GATC	3 cut(s)	183, 813, 1000
Sau96I	GGNCC	1 cut(s)	124
ScaI	AGTACT	2 cut(s)	154, 586
SchI	GAGTC	2 cut(s)	65, 1114
ScrFI	CCNGG	3 cut(s)	368, 665, 1269
SduI	GDGCHC	4 cut(s)	9, 316, 806, 1014
SfcI	CTRYAG	2 cut(s)	112, 1260
Sfr274I	CTCGAG	1 cut(s)	1007
SfuI	TTCGAA	1 cut(s)	670
SlaI	CTCGAG	1 cut(s)	1007
SmlI	CTYRAG	2 cut(s)	916, 1007
SmoI	CTYRAG	2 cut(s)	916, 1007
SpeI	ACTAGT	2 cut(s)	581, 907
Sse9I	AATT	8 cut(s)	317, 592, 603, 672, 748, 765, 971, 1129
SsiI	CCGC	5 cut(s)	648, 714, 723, 862, 1211
SspI	AATATT	1 cut(s)	744
SspMI	CTAG	3 cut(s)	582, 908, 1019
SstI	GAGCTC	1 cut(s)	316
StyD4I	CCNGG	3 cut(s)	366, 663, 1267
StyI	CCWWGG	1 cut(s)	83
TaaI	ACNGT	7 cut(s)	34, 349, 982, 994, 1173, 1187, 1261
TaiI	ACGT	2 cut(s)	12, 218
TaqI	TCGA	5 cut(s)	72, 294, 363, 670, 1008
TasI	AATT	8 cut(s)	317, 592, 603, 672, 748, 765, 971, 1129
TatI	WGTACW	2 cut(s)	152, 584
TauI	GCSGC	1 cut(s)	1213
TfiI	GAWTC	3 cut(s)	69, 651, 927
Tru1I	TTAA	3 cut(s)	77, 747, 917
Tru9I	TTAA	3 cut(s)	77, 747, 917
TscAI	CASTG	4 cut(s)	37, 388, 997, 1326
TseFI	GTSAC	4 cut(s)	343, 383, 982, 1308
TseI	GCWGC	2 cut(s)	610, 1232
Tsp45I	GTSAC	4 cut(s)	343, 383, 982, 1308
TspDTI	ATGAA	5 cut(s)	88, 444, 478, 722, 1339
TspGWI	ACGGA	3 cut(s)	280, 1029, 1234
TspRI	CASTG	4 cut(s)	37, 388, 997, 1326
Vha464I	CTTAAG	1 cut(s)	916
XapI	RAATTY	1 cut(s)	603
XcmI	CCANNNNNNNNNTGG	2 cut(s)	796, 1243
XhoI	CTCGAG	1 cut(s)	1007
XmiI	GTMKAC	1 cut(s)	899
XspI	CTAG	3 cut(s)	582, 908, 1019
ZrmI	AGTACT	2 cut(s)	154, 586

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.