MD05G1179800.v1.1

Alcohol dehydrogenase GroES-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000815
Physical Location & Seq
Forward (+)
30774725 .. 30786577
11853 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1179800.v1.1.491

Sequence Viewer

Length: 1143 bp
ATGTCGTCTACAGCCGGCCTTGTGATCCCCTGCACAGCGGCGGTTGCATGGGAGGCAGGGAAGCCATTGGTGATGGAGAGGGTGGAGGTTGCTCCACCTCAGGCCATGGAAGTTAGGGTCAAGATCAAATATACTTCACTTTGCCACACTGATCTCTACTTCTGGGAAGCCAAGGGCCAGACACCTTTGTTCCCTCGAATTTTCGGCCATGAAGCATCTGGAATCGTTGAGAGTGTTGGGGAAGGCGTTGAAGGCCTCGAGGTTGGTGATCATGTACTACCTGTTTTCACTGGGGAGTGTGGGGATTGTGCGCATTGCAAGTCTGAGGAAAGCAACATGTGTGACCTGTTAAGAATAAATACAGATAGAGGGGTCATGCTAAGTGATGAGAAACCGAGGTTTTCTATAAATGGGACTCCGATTAATCACTTCGTCGGCACATCCACCTTCAGTGAATACACTGTTATTCATTCCGGCTGCCTTGCCAAGATCAATCCCCTGGCTCCTTTGGATATAGTTTGCATCCTCAGCTGTGGCATCACGACAGGTTTGGGTGCCACTCTGAATGTAGCAAAACCGAAAAAGGGATCTTCAGTTGCTATCTTTGGACTTGGGGCTGTTGGGCTGGCTGCTGCTGAAGGTGCAAGAATTTCTGGGGCGTCGCGGATTATTGGGGTTGACAGGAATCCCAAGAGGTTTGAGGAAGCCAAGAAATTTGGAGTGAATGAGTTTGTGAATCCAAAGGACCATGACAAACCAGTCCAGGAGGTTATTGCTGAGATGACCAATGGTGGAGCTGACAGAAGCATAGAGTGCACGGGCAATATTAACGCCATGATCTCGGCTTTTGAATGTGTTCATGATGGTTGGGGTGTGGCTGTGCTTGTTGGAGTTCCGAACAAGGATGCAGTCTTCATGACTAAGCCCATTAATTTGCTTAATGAAAGGACGCTTAAAGGAACTTTTTTTGGGAACTACAAACCTCGGACAGACCTTCCTTCTGTTGTGGAAATGTACATGAGCAAGAAATTGGAAGTAGAGAAGTTTATAACGCATCGGGTGCCTTTCTCAGAGATCAATAAAGCCTTTGATTACATGATAAGGGGAGAAGGCTTGCGGTGCATCGTTAGCATGGAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00071 ko00350 ko00592 ko01100 ko01110 ko01120 map00010 map00071 map00350 map00592 map01100 map01110 map01120
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

381

Amino Acids

41.16

Weight (kDa)

5.8

Isoelectric Point (pI)

31.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 36 - 164 4.8e-22 Alcohol dehydrogenase GroES-like domain
Glu_dehyd_C PF16912 197 - 364 3.8e-06 Glucose dehydrogenase C-terminus
ADH_zinc_N PF00107 206 - 338 6.1e-28 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1049
AasI GACNNNNNNGTC 1 cut(s) 758
AbsI CCTCGAGG 1 cut(s) 257
Acc16I TGCGCA 1 cut(s) 312
AccB1I GGYRCC 2 cut(s) 554, 1060
AccI GTMKAC 1 cut(s) 8
AccII CGCG 1 cut(s) 664
AciI CCGC 4 cut(s) 38, 41, 664, 1117
AclWI GGATC 2 cut(s) 19, 595
AcoI YGGCCR 1 cut(s) 205
AcsI RAATTY 3 cut(s) 198, 648, 713
AcuI CTGAAG 3 cut(s) 433, 576, 657
AcyI GRCGYC 1 cut(s) 659
AfaI GTAC 2 cut(s) 276, 1016
AfiI CCNNNNNNNGG 1 cut(s) 584
AflIII ACRYGT 1 cut(s) 336
AgsI TTSAA 2 cut(s) 251, 851
AjnI CCWGG 2 cut(s) 498, 762
AluBI AGCT 2 cut(s) 531, 797
AluI AGCT 2 cut(s) 531, 797
Alw21I GWGCWC 1 cut(s) 818
Alw44I GTGCAC 1 cut(s) 814
AlwI GGATC 2 cut(s) 19, 595
Ama87I CYCGRG 1 cut(s) 257
AoxI GGCC 5 cut(s) 16, 102, 175, 205, 253
ApaLI GTGCAC 1 cut(s) 814
ApeKI GCWGC 3 cut(s) 477, 629, 632
ApoI RAATTY 3 cut(s) 198, 648, 713
AseI ATTAAT 2 cut(s) 423, 930
Asp700I GAANNNNTTC 1 cut(s) 855
AspLEI GCGC 1 cut(s) 313
AspS9I GGNCC 2 cut(s) 175, 745
AsuHPI GGTGA 2 cut(s) 82, 278
AvaI CYCGRG 1 cut(s) 257
AvaII GGWCC 1 cut(s) 745
AxyI CCTNAGG 1 cut(s) 99
BaeGI GKGCMC 1 cut(s) 818
BanI GGYRCC 2 cut(s) 554, 1060
BbsI GAAGAC 1 cut(s) 904
Bbv12I GWGCWC 1 cut(s) 818
BbvCI CCTCAGC 1 cut(s) 527
BbvI GCAGC 3 cut(s) 464, 616, 619
BccI CCATC 2 cut(s) 67, 857
BciT130I CCWGG 2 cut(s) 500, 764
BclI TGATCA 1 cut(s) 268
BfmI CTRYAG 1 cut(s) 9
BisI GCNGC 4 cut(s) 39, 478, 630, 633
BlsI GCNGC 4 cut(s) 40, 479, 631, 634
Bme1390I CCNGG 2 cut(s) 500, 764
Bme18I GGWCC 1 cut(s) 745
BmeT110I CYCGRG 1 cut(s) 257
BmgT120I GGNCC 2 cut(s) 175, 745
BmiI GGNNCC 3 cut(s) 504, 556, 1062
BmrFI CCNGG 2 cut(s) 500, 764
BmrI ACTGGG 1 cut(s) 300
BmsI GCATC 6 cut(s) 224, 531, 546, 895, 1063, 1131
BmuI ACTGGG 1 cut(s) 300
BpiI GAAGAC 1 cut(s) 904
Bpu10I CCTNAGC 1 cut(s) 527
BsaHI GRCGYC 1 cut(s) 659
BsaJI CCNNGG 5 cut(s) 105, 171, 395, 498, 983
Bsc4I CCNNNNNNNGG 1 cut(s) 584
Bse118I RCCGGY 1 cut(s) 14
Bse1I ACTGG 2 cut(s) 295, 758
Bse21I CCTNAGG 1 cut(s) 99
Bse3DI GCAATG 1 cut(s) 313
BseBI CCWGG 2 cut(s) 500, 764
BseDI CCNNGG 5 cut(s) 105, 171, 395, 498, 983
BseGI GGATG 3 cut(s) 440, 522, 910
BseLI CCNNNNNNNGG 1 cut(s) 584
BseMI GCAATG 1 cut(s) 313
BseMII CTCAG 5 cut(s) 113, 315, 541, 768, 1083
BseNI ACTGG 2 cut(s) 295, 758
BseSI GKGCMC 1 cut(s) 818
BseXI GCAGC 3 cut(s) 464, 616, 619
BsgI GTGCAG 1 cut(s) 16
Bsh1236I CGCG 1 cut(s) 664
BshFI GGCC 5 cut(s) 18, 104, 177, 207, 255
BshNI GGYRCC 2 cut(s) 554, 1060
BsiHKAI GWGCWC 1 cut(s) 818
BsiHKCI CYCGRG 1 cut(s) 257
BsiSI CCGG 2 cut(s) 15, 474
BslFI GGGAC 1 cut(s) 427
BslI CCNNNNNNNGG 1 cut(s) 584
BsmFI GGGAC 1 cut(s) 427
BsnI GGCC 5 cut(s) 18, 104, 177, 207, 255
BsoBI CYCGRG 1 cut(s) 257
Bsp1286I GDGCHC 1 cut(s) 818
Bsp1407I TGTACA 1 cut(s) 1014
Bsp143I GATC 8 cut(s) 24, 123, 151, 268, 489, 587, 837, 1074
Bsp19I CCATGG 1 cut(s) 105
BspACI CCGC 4 cut(s) 38, 41, 664, 1117
BspANI GGCC 5 cut(s) 18, 104, 177, 207, 255
BspCNI CTCAG 5 cut(s) 112, 316, 540, 769, 1082
BspFNI CGCG 1 cut(s) 664
BspHI TCATGA 2 cut(s) 859, 915
BspLI GGNNCC 3 cut(s) 504, 556, 1062
BspPI GGATC 2 cut(s) 19, 595
BspT107I GGYRCC 2 cut(s) 554, 1060
BsrDI GCAATG 1 cut(s) 313
BsrFI RCCGGY 1 cut(s) 14
BsrGI TGTACA 1 cut(s) 1014
BsrI ACTGG 2 cut(s) 295, 758
BssAI RCCGGY 1 cut(s) 14
BssECI CCNNGG 5 cut(s) 105, 171, 395, 498, 983
BssMI GATC 8 cut(s) 24, 123, 151, 268, 489, 587, 837, 1074
BssNI GRCGYC 1 cut(s) 659
BssT1I CCWWGG 2 cut(s) 105, 171
Bst2UI CCWGG 2 cut(s) 500, 764
Bst4CI ACNGT 1 cut(s) 463
Bst6I CTCTTC 1 cut(s) 1131
BstACI GRCGYC 1 cut(s) 659
BstAPI GCANNNNNTGC 2 cut(s) 813, 1060
BstAUI TGTACA 1 cut(s) 1014
BstC8I GCNNGC 3 cut(s) 16, 627, 1115
BstDEI CTNAG 7 cut(s) 99, 324, 380, 527, 777, 921, 1069
BstDSI CCRYGG 1 cut(s) 105
BstF5I GGATG 3 cut(s) 440, 522, 910
BstFNI CGCG 1 cut(s) 664
BstHHI GCGC 1 cut(s) 313
BstKTI GATC 8 cut(s) 27, 126, 154, 271, 492, 590, 840, 1077
BstMBI GATC 8 cut(s) 24, 123, 151, 268, 489, 587, 837, 1074
BstMWI GCNNNNNNNGC 9 cut(s) 44, 53, 252, 528, 641, 813, 1060, 1119, 1128
BstNI CCWGG 2 cut(s) 500, 764
BstNSI RCATGY 1 cut(s) 340
BstSCI CCNGG 2 cut(s) 498, 762
BstSFI CTRYAG 1 cut(s) 9
BstSLI GKGCMC 1 cut(s) 818
BstUI CGCG 1 cut(s) 664
BstV1I GCAGC 3 cut(s) 464, 616, 619
BstV2I GAAGAC 1 cut(s) 904
BstX2I RGATCY 1 cut(s) 587
BstYI RGATCY 1 cut(s) 587
Bsu36I CCTNAGG 1 cut(s) 99
BsuRI GGCC 5 cut(s) 18, 104, 177, 207, 255
BtgI CCRYGG 1 cut(s) 105
BtsCI GGATG 3 cut(s) 440, 522, 910
BtsIMutI CAGTG 4 cut(s) 147, 288, 457, 459
Cac8I GCNNGC 3 cut(s) 16, 627, 1115
CciI TCATGA 2 cut(s) 859, 915
CfoI GCGC 1 cut(s) 313
Cfr10I RCCGGY 1 cut(s) 14
Cfr13I GGNCC 2 cut(s) 175, 745
CseI GACGC 2 cut(s) 648, 958
Csp6I GTAC 2 cut(s) 275, 1015
CviQI GTAC 2 cut(s) 275, 1015
DdeI CTNAG 7 cut(s) 99, 324, 380, 527, 777, 921, 1069
DpnI GATC 8 cut(s) 26, 125, 153, 270, 491, 589, 839, 1076
DpnII GATC 8 cut(s) 24, 123, 151, 268, 489, 587, 837, 1074
DrdI GACNNNNNNGTC 1 cut(s) 758
DseDI GACNNNNNNGTC 1 cut(s) 758
EaeI YGGCCR 1 cut(s) 205
Eam1104I CTCTTC 1 cut(s) 1131
EarI CTCTTC 1 cut(s) 1131
Eco130I CCWWGG 2 cut(s) 105, 171
Eco147I AGGCCT 1 cut(s) 255
Eco47I GGWCC 1 cut(s) 745
Eco57I CTGAAG 3 cut(s) 433, 576, 657
Eco81I CCTNAGG 1 cut(s) 99
Eco88I CYCGRG 1 cut(s) 257
EcoRII CCWGG 2 cut(s) 498, 762
EcoT14I CCWWGG 2 cut(s) 105, 171
ErhI CCWWGG 2 cut(s) 105, 171
FaqI GGGAC 1 cut(s) 427
FbaI TGATCA 1 cut(s) 268
FblI GTMKAC 1 cut(s) 8
Fnu4HI GCNGC 4 cut(s) 39, 478, 630, 633
FokI GGATG 3 cut(s) 427, 509, 917
Fsp4HI GCNGC 4 cut(s) 39, 478, 630, 633
FspAI RTGCGCAY 1 cut(s) 312
FspI TGCGCA 1 cut(s) 312
GlaI GCGC 1 cut(s) 312
GluI GCNGC 4 cut(s) 39, 478, 630, 633
HaeIII GGCC 5 cut(s) 18, 104, 177, 207, 255
HapII CCGG 2 cut(s) 15, 474
HgaI GACGC 2 cut(s) 648, 958
HhaI GCGC 1 cut(s) 313
Hin1I GRCGYC 1 cut(s) 659
Hin6I GCGC 1 cut(s) 311
HinP1I GCGC 1 cut(s) 311
HincII GTYRAC 1 cut(s) 679
HindII GTYRAC 1 cut(s) 679
HinfI GANTC 4 cut(s) 222, 415, 685, 736
HpaII CCGG 2 cut(s) 15, 474
HphI GGTGA 2 cut(s) 82, 278
Hpy166II GTNNAC 3 cut(s) 9, 679, 816
Hpy188I TCNGA 6 cut(s) 325, 420, 564, 897, 987, 1072
Hpy188III TCNNGA 5 cut(s) 121, 219, 541, 860, 916
Hpy8I GTNNAC 3 cut(s) 9, 679, 816
Hpy99I CGWCG 2 cut(s) 437, 664
HpyAV CCTTC 7 cut(s) 236, 245, 457, 632, 1004, 1008, 1103
HpyCH4III ACNGT 1 cut(s) 463
HpyCH4V TGCA 8 cut(s) 33, 47, 318, 522, 644, 816, 908, 1122
HpyF10VI GCNNNNNNNGC 9 cut(s) 44, 53, 252, 528, 641, 813, 1060, 1119, 1128
HpyF3I CTNAG 7 cut(s) 99, 324, 380, 527, 777, 921, 1069
Hsp92I GRCGYC 1 cut(s) 659
HspAI GCGC 1 cut(s) 311
KroI GCCGGC 1 cut(s) 14
KroNI GCCGGC 1 cut(s) 16
Ksp22I TGATCA 1 cut(s) 268
Kzo9I GATC 8 cut(s) 24, 123, 151, 268, 489, 587, 837, 1074
LmnI GCTCC 3 cut(s) 97, 508, 794
Lsp1109I GCAGC 3 cut(s) 464, 616, 619
LweI GCATC 6 cut(s) 224, 531, 546, 895, 1063, 1131
MaeIII GTNAC 1 cut(s) 341
MalI GATC 8 cut(s) 26, 125, 153, 270, 491, 589, 839, 1076
MboI GATC 8 cut(s) 24, 123, 151, 268, 489, 587, 837, 1074
MboII GAAGA 2 cut(s) 582, 904
MflI RGATCY 1 cut(s) 587
MhlI GDGCHC 1 cut(s) 818
MluCI AATT 5 cut(s) 198, 648, 713, 931, 1028
MlyI GAGTC 1 cut(s) 409
MmeI TCCRAC 1 cut(s) 868
MroNI GCCGGC 1 cut(s) 14
MroXI GAANNNNTTC 1 cut(s) 855
MseI TTAA 6 cut(s) 350, 423, 828, 930, 939, 954
MspA1I CMGCKG 2 cut(s) 38, 531
MspI CCGG 2 cut(s) 15, 474
MspR9I CCNGG 2 cut(s) 500, 764
MvaI CCWGG 2 cut(s) 500, 764
MvnI CGCG 1 cut(s) 664
MwoI GCNNNNNNNGC 9 cut(s) 44, 53, 252, 528, 641, 813, 1060, 1119, 1128
NaeI GCCGGC 1 cut(s) 16
NcoI CCATGG 1 cut(s) 105
NdeII GATC 8 cut(s) 24, 123, 151, 268, 489, 587, 837, 1074
NgoMIV GCCGGC 1 cut(s) 14
NlaIV GGNNCC 3 cut(s) 504, 556, 1062
NmeAIII GCCGAG 1 cut(s) 821
NmuCI GTSAC 1 cut(s) 341
NsbI TGCGCA 1 cut(s) 312
NspI RCATGY 1 cut(s) 340
PaeR7I CTCGAG 1 cut(s) 257
PagI TCATGA 2 cut(s) 859, 915
PceI AGGCCT 1 cut(s) 255
PciI ACATGT 1 cut(s) 336
PdiI GCCGGC 1 cut(s) 16
PdmI GAANNNNTTC 1 cut(s) 855
PfeI GAWTC 3 cut(s) 222, 685, 736
PfoI TCCNGGA 1 cut(s) 762
PkrI GCNGC 4 cut(s) 40, 479, 631, 634
PleI GAGTC 1 cut(s) 409
PpsI GAGTC 1 cut(s) 409
PscI ACATGT 1 cut(s) 336
PshBI ATTAAT 2 cut(s) 423, 930
PsiI TTATAA 1 cut(s) 1049
Psp6I CCWGG 2 cut(s) 498, 762
PspGI CCWGG 2 cut(s) 498, 762
PspN4I GGNNCC 3 cut(s) 504, 556, 1062
PspPI GGNCC 2 cut(s) 175, 745
PspXI VCTCGAGB 1 cut(s) 257
PsuI RGATCY 1 cut(s) 587
PvuII CAGCTG 1 cut(s) 531
RsaI GTAC 2 cut(s) 276, 1016
RsaNI GTAC 2 cut(s) 275, 1015
SaqAI TTAA 6 cut(s) 350, 423, 828, 930, 939, 954
SatI GCNGC 4 cut(s) 39, 478, 630, 633
Sau3AI GATC 8 cut(s) 24, 123, 151, 268, 489, 587, 837, 1074
Sau96I GGNCC 2 cut(s) 175, 745
SchI GAGTC 1 cut(s) 409
ScrFI CCNGG 2 cut(s) 500, 764
SduI GDGCHC 1 cut(s) 818
SfaNI GCATC 6 cut(s) 224, 531, 546, 895, 1063, 1131
SfcI CTRYAG 1 cut(s) 9
Sfr274I CTCGAG 1 cut(s) 257
SinI GGWCC 1 cut(s) 745
SlaI CTCGAG 1 cut(s) 257
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
Sse9I AATT 5 cut(s) 198, 648, 713, 931, 1028
SseBI AGGCCT 1 cut(s) 255
SsiI CCGC 4 cut(s) 38, 41, 664, 1117
SspI AATATT 1 cut(s) 826
StuI AGGCCT 1 cut(s) 255
StyD4I CCNGG 2 cut(s) 498, 762
StyI CCWWGG 2 cut(s) 105, 171
TaaI ACNGT 1 cut(s) 463
TaqI TCGA 2 cut(s) 196, 258
TasI AATT 5 cut(s) 198, 648, 713, 931, 1028
TatI WGTACW 2 cut(s) 274, 1014
TauI GCSGC 1 cut(s) 41
TfiI GAWTC 3 cut(s) 222, 685, 736
Tru1I TTAA 6 cut(s) 350, 423, 828, 930, 939, 954
Tru9I TTAA 6 cut(s) 350, 423, 828, 930, 939, 954
TscAI CASTG 4 cut(s) 154, 295, 457, 466
TseFI GTSAC 1 cut(s) 341
TseI GCWGC 3 cut(s) 477, 629, 632
Tsp45I GTSAC 1 cut(s) 341
TspDTI ATGAA 5 cut(s) 225, 458, 848, 904, 957
TspRI CASTG 4 cut(s) 154, 295, 457, 466
VneI GTGCAC 1 cut(s) 814
VpaK11BI GGWCC 1 cut(s) 745
VspI ATTAAT 2 cut(s) 423, 930
XapI RAATTY 3 cut(s) 198, 648, 713
XceI RCATGY 1 cut(s) 340
XcmI CCANNNNNNNNNTGG 1 cut(s) 215
XhoI CTCGAG 1 cut(s) 257
XmiI GTMKAC 1 cut(s) 8
XmnI GAANNNNTTC 1 cut(s) 855
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.