RLG00000013310

alcohol dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000815
Physical Location & Seq
Reverse (-)
29521933 .. 29524187
2255 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013310

Sequence Viewer

Length: 1143 bp
ATGTCCAGTACTGAAGGAAAGGTCATATGCTGCAGAGCTGCTGTGGCATGGGAAGCAGGGAAGCCATTAGTGATTGAAGAAGTAGAGGTAGCACCTCCTCAGGCCAATGAAGTTCGTGTCAAGATTCTTTATACCTCCTTGTGTCATACTGATGTCTACTTCTGGGAAGCCAAGGGACAAAACCCTTTATTTCCAAGGATTTACGGCCATGAGGCAGGAGGGATTGTGGAGAGTGTTGGTGAAGGAGTGACTGATCTCAAACCGGGAGATCATGTGCTCCCGGTGTTCACTGGGGAATGCCAGGAGTGTGCTCACTGTAAATCAGAAGAGAGCAACATGTGTGACCTCCTCAGGATAAACACAGACAGGGGAGTGATGCTTAATGATGGACAATCGAGGTTTTCGATTAAGGGCAAACCTATATACCACTTTGTTGGGACTTCAACGTTTAGTGAGTACACTGTGATTCATGTTGGCTGCCTTGCCAAGATCAACCCCAAAGCTCCTCTAGACAAAGTTTGTGTCCTCAGTTGTGGAATCTCAACTGGTTTGGGAGCTACTCTTAATGTAGCAAAACCGAGAAAGGGCTCAACAGTGGCTGTGTTTGGATTGGGAGCTGTAGGCCTTGCAGCTGCTGAAGGAGCCAGGATGGCTGGGGCTTCAAGAATTATTGGTGTTGATTTGAATTCGAGCAGATTTGAAGAAGCTAAAAAGTTTGGTATTACTGAATTTGTGAACCCAAAAGACCACAAAAAACCAGTTCAAGAGGTGATTGCTGAGCTGACGGATGGAGGAGTTGATCGAAGCATTGAATGCACTGGAAATATTCAAGCCATGATATCTGCATTTGAATGTGTCCATGATGGTTGGGGTGTTGCTGTTCTTGTGGGAGTGCCACACAAAGATGCTGTCTTCCAGACTCATCCAGTGAACTTCCTGAATGAGAGGACTCTCAAGGGTACCTTCTTCGGAAACTACAAACCACGGACCGACATTCCCTCTGTTGTGGAGAAATACATGAACAAGGAACTGGAGCTAGACAAATTCATCACCCATCAAGTCCCATTCTCAGAAATCAACAAGGCATTTGACTACATGCTTAAAGGGGAAGGTCTTCGTTGCATAATTAACATGGAGGGGTGA

Protein Analysis

381

Amino Acids

41.33

Weight (kDa)

6.03

Isoelectric Point (pI)

34.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 35 - 164 1.7e-20 Alcohol dehydrogenase GroES-like domain
Glu_dehyd_C PF16912 197 - 364 6.2e-06 Glucose dehydrogenase C-terminus
ADH_zinc_N PF00107 206 - 337 8.4e-29 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 959
AccB1I GGYRCC 1 cut(s) 959
AccI GTMKAC 1 cut(s) 156
AclI AACGTT 1 cut(s) 446
AcoI YGGCCR 1 cut(s) 205
AcsI RAATTY 3 cut(s) 685, 728, 1043
AcuI CTGAAG 2 cut(s) 33, 657
AfaI GTAC 3 cut(s) 10, 458, 961
AfiI CCNNNNNNNGG 1 cut(s) 584
AflIII ACRYGT 1 cut(s) 336
AgsI TTSAA 9 cut(s) 77, 444, 663, 685, 701, 764, 812, 830, 851
AjnI CCWGG 2 cut(s) 300, 644
AluBI AGCT 8 cut(s) 38, 503, 557, 617, 632, 707, 781, 1036
AluI AGCT 8 cut(s) 38, 503, 557, 617, 632, 707, 781, 1036
Alw21I GWGCWC 2 cut(s) 279, 313
AlwNI CAGNNNCTG 2 cut(s) 599, 635
AoxI GGCC 3 cut(s) 102, 205, 622
ApeKI GCWGC 5 cut(s) 30, 38, 477, 629, 632
ApoI RAATTY 3 cut(s) 685, 728, 1043
ArsI GACNNNNNNTTYG 4 cut(s) 507, 539, 894, 926
Asp700I GAANNNNTTC 1 cut(s) 1113
Asp718I GGTACC 1 cut(s) 959
AspS9I GGNCC 1 cut(s) 987
AsuC2I CCSGG 2 cut(s) 264, 281
AsuHPI GGTGA 3 cut(s) 251, 781, 1042
AvaII GGWCC 1 cut(s) 987
AxyI CCTNAGG 2 cut(s) 99, 350
BanI GGYRCC 1 cut(s) 959
BanII GRGCYC 1 cut(s) 590
BbsI GAAGAC 2 cut(s) 904, 1106
Bbv12I GWGCWC 2 cut(s) 279, 313
BbvI GCAGC 5 cut(s) 17, 25, 464, 619, 641
BccI CCATC 5 cut(s) 380, 643, 782, 857, 1062
BceAI ACGGC 1 cut(s) 220
BciT130I CCWGG 2 cut(s) 302, 646
BcnI CCSGG 2 cut(s) 264, 281
BfaI CTAG 2 cut(s) 509, 1037
BfmI CTRYAG 2 cut(s) 31, 618
BglI GCCNNNNNGGC 1 cut(s) 650
BisI GCNGC 5 cut(s) 31, 39, 478, 630, 633
BlpI GCTNAGC 1 cut(s) 777
BlsI GCNGC 5 cut(s) 32, 40, 479, 631, 634
BmcAI AGTACT 1 cut(s) 10
Bme1390I CCNGG 4 cut(s) 264, 281, 302, 646
Bme18I GGWCC 1 cut(s) 987
BmgT120I GGNCC 1 cut(s) 987
BmiI GGNNCC 2 cut(s) 643, 961
BmrFI CCNGG 4 cut(s) 264, 281, 302, 646
BmrI ACTGGG 1 cut(s) 300
BmsI GCATC 2 cut(s) 366, 895
BmuI ACTGGG 1 cut(s) 300
BpiI GAAGAC 2 cut(s) 904, 1106
BpmI CTGGAG 1 cut(s) 1052
Bpu1102I GCTNAGC 1 cut(s) 777
BpuEI CTTGAG 1 cut(s) 938
BpuMI CCSGG 2 cut(s) 264, 281
BsaJI CCNNGG 3 cut(s) 171, 194, 983
BsaXI ACNNNNNCTCC 4 cut(s) 210, 240, 258, 288
Bsc4I CCNNNNNNNGG 1 cut(s) 584
Bse1I ACTGG 7 cut(s) 6, 295, 550, 758, 823, 926, 1035
Bse21I CCTNAGG 2 cut(s) 99, 350
BseBI CCWGG 2 cut(s) 302, 646
BseDI CCNNGG 3 cut(s) 171, 194, 983
BseGI GGATG 3 cut(s) 654, 793, 922
BseLI CCNNNNNNNGG 1 cut(s) 584
BseMII CTCAG 5 cut(s) 113, 364, 541, 768, 1083
BseNI ACTGG 7 cut(s) 6, 295, 550, 758, 823, 926, 1035
BseRI GAGGAG 4 cut(s) 87, 338, 495, 807
BseXI GCAGC 5 cut(s) 17, 25, 464, 619, 641
BseYI CCCAGC 1 cut(s) 653
BshFI GGCC 3 cut(s) 104, 207, 624
BshNI GGYRCC 1 cut(s) 959
BsiHKAI GWGCWC 2 cut(s) 279, 313
BsiSI CCGG 2 cut(s) 263, 281
BslFI GGGAC 3 cut(s) 189, 451, 1046
BslI CCNNNNNNNGG 1 cut(s) 584
BsmFI GGGAC 3 cut(s) 189, 451, 1046
BsmI GAATGC 2 cut(s) 302, 818
BsnI GGCC 3 cut(s) 104, 207, 624
Bsp1286I GDGCHC 3 cut(s) 279, 313, 590
Bsp143I GATC 4 cut(s) 253, 268, 489, 799
Bsp1720I GCTNAGC 1 cut(s) 777
BspANI GGCC 3 cut(s) 104, 207, 624
BspCNI CTCAG 5 cut(s) 112, 363, 540, 769, 1082
BspLI GGNNCC 2 cut(s) 643, 961
BspMAI CTGCAG 1 cut(s) 35
BspT107I GGYRCC 1 cut(s) 959
BsrI ACTGG 7 cut(s) 6, 295, 550, 758, 823, 926, 1035
BssECI CCNNGG 3 cut(s) 171, 194, 983
BssMI GATC 4 cut(s) 253, 268, 489, 799
BssT1I CCWWGG 2 cut(s) 171, 194
Bst2UI CCWGG 2 cut(s) 302, 646
Bst4CI ACNGT 3 cut(s) 317, 463, 595
Bst6I CTCTTC 1 cut(s) 321
BstAPI GCANNNNNTGC 1 cut(s) 813
BstDEI CTNAG 5 cut(s) 99, 350, 527, 777, 1069
BstDSI CCRYGG 1 cut(s) 983
BstF5I GGATG 3 cut(s) 654, 793, 922
BstKTI GATC 4 cut(s) 256, 271, 492, 802
BstMBI GATC 4 cut(s) 253, 268, 489, 799
BstMWI GCNNNNNNNGC 5 cut(s) 44, 53, 641, 650, 813
BstNI CCWGG 2 cut(s) 302, 646
BstNSI RCATGY 2 cut(s) 340, 1099
BstSCI CCNGG 4 cut(s) 262, 279, 300, 644
BstSFI CTRYAG 2 cut(s) 31, 618
BstV1I GCAGC 5 cut(s) 17, 25, 464, 619, 641
BstV2I GAAGAC 2 cut(s) 904, 1106
BstXI CCANNNNNNTGG 1 cut(s) 434
Bsu36I CCTNAGG 2 cut(s) 99, 350
BsuRI GGCC 3 cut(s) 104, 207, 624
BtgI CCRYGG 1 cut(s) 983
BtsCI GGATG 3 cut(s) 654, 793, 922
BtsIMutI CAGTG 6 cut(s) 288, 313, 459, 600, 816, 933
CaiI CAGNNNCTG 2 cut(s) 599, 635
Cfr13I GGNCC 1 cut(s) 987
CpoI CGGWCCG 1 cut(s) 987
Csp6I GTAC 3 cut(s) 9, 457, 960
CspI CGGWCCG 1 cut(s) 987
CviQI GTAC 3 cut(s) 9, 457, 960
DdeI CTNAG 5 cut(s) 99, 350, 527, 777, 1069
DpnI GATC 4 cut(s) 255, 270, 491, 801
DpnII GATC 4 cut(s) 253, 268, 489, 799
EaeI YGGCCR 1 cut(s) 205
Eam1104I CTCTTC 1 cut(s) 321
EarI CTCTTC 1 cut(s) 321
Eco130I CCWWGG 2 cut(s) 171, 194
Eco147I AGGCCT 1 cut(s) 624
Eco24I GRGCYC 1 cut(s) 590
Eco32I GATATC 1 cut(s) 840
Eco47I GGWCC 1 cut(s) 987
Eco57I CTGAAG 2 cut(s) 33, 657
Eco81I CCTNAGG 2 cut(s) 99, 350
EcoRI GAATTC 1 cut(s) 685
EcoRII CCWGG 2 cut(s) 300, 644
EcoRV GATATC 1 cut(s) 840
EcoT14I CCWWGG 2 cut(s) 171, 194
EcoT38I GRGCYC 1 cut(s) 590
ErhI CCWWGG 2 cut(s) 171, 194
FalI AAGNNNNNCTT 2 cut(s) 947, 979
FaqI GGGAC 3 cut(s) 189, 451, 1046
FauNDI CATATG 1 cut(s) 26
FblI GTMKAC 1 cut(s) 156
Fnu4HI GCNGC 5 cut(s) 31, 39, 478, 630, 633
FokI GGATG 3 cut(s) 661, 800, 909
FriOI GRGCYC 1 cut(s) 590
Fsp4HI GCNGC 5 cut(s) 31, 39, 478, 630, 633
FspBI CTAG 2 cut(s) 509, 1037
GluI GCNGC 5 cut(s) 31, 39, 478, 630, 633
GsaI CCCAGC 1 cut(s) 657
GsuI CTGGAG 1 cut(s) 1052
HaeIII GGCC 3 cut(s) 104, 207, 624
HapII CCGG 2 cut(s) 263, 281
HinfI GANTC 5 cut(s) 124, 466, 537, 919, 949
HpaII CCGG 2 cut(s) 263, 281
HphI GGTGA 3 cut(s) 251, 781, 1042
Hpy166II GTNNAC 5 cut(s) 157, 288, 459, 736, 931
Hpy188I TCNGA 3 cut(s) 325, 971, 1072
Hpy188III TCNNGA 7 cut(s) 121, 352, 509, 663, 764, 916, 937
Hpy8I GTNNAC 5 cut(s) 157, 288, 459, 736, 931
HpyAV CCTTC 5 cut(s) 8, 236, 632, 973, 1103
HpyCH4III ACNGT 3 cut(s) 317, 463, 595
HpyCH4IV ACGT 1 cut(s) 446
HpyCH4V TGCA 5 cut(s) 33, 629, 816, 845, 1122
HpyF10VI GCNNNNNNNGC 5 cut(s) 44, 53, 641, 650, 813
HpyF3I CTNAG 5 cut(s) 99, 350, 527, 777, 1069
HpySE526I ACGT 1 cut(s) 446
KpnI GGTACC 1 cut(s) 963
Kzo9I GATC 4 cut(s) 253, 268, 489, 799
LmnI GCTCC 6 cut(s) 282, 508, 554, 614, 641, 1033
Lsp1109I GCAGC 5 cut(s) 17, 25, 464, 619, 641
LweI GCATC 2 cut(s) 366, 895
MaeI CTAG 2 cut(s) 509, 1037
MaeII ACGT 1 cut(s) 446
MaeIII GTNAC 2 cut(s) 247, 341
MalI GATC 4 cut(s) 255, 270, 491, 801
MboI GATC 4 cut(s) 253, 268, 489, 799
MboII GAAGA 6 cut(s) 89, 338, 713, 904, 958, 1106
MhlI GDGCHC 3 cut(s) 279, 313, 590
MluCI AATT 5 cut(s) 666, 685, 728, 1043, 1125
MlyI GAGTC 2 cut(s) 913, 943
MroXI GAANNNNTTC 1 cut(s) 1113
MseI TTAA 5 cut(s) 381, 408, 564, 1101, 1128
MslI CAYNNNNRTG 3 cut(s) 150, 850, 903
MspA1I CMGCKG 1 cut(s) 632
MspI CCGG 2 cut(s) 263, 281
MspR9I CCNGG 4 cut(s) 264, 281, 302, 646
Mva1269I GAATGC 2 cut(s) 302, 818
MvaI CCWGG 2 cut(s) 302, 646
MwoI GCNNNNNNNGC 5 cut(s) 44, 53, 641, 650, 813
NciI CCSGG 2 cut(s) 264, 281
NdeI CATATG 1 cut(s) 26
NdeII GATC 4 cut(s) 253, 268, 489, 799
NlaIV GGNNCC 2 cut(s) 643, 961
NmuCI GTSAC 2 cut(s) 247, 341
NspI RCATGY 2 cut(s) 340, 1099
PceI AGGCCT 1 cut(s) 624
PciI ACATGT 1 cut(s) 336
PcsI WCGNNNNNNNCGW 1 cut(s) 401
PctI GAATGC 2 cut(s) 302, 818
PdmI GAANNNNTTC 1 cut(s) 1113
PfeI GAWTC 3 cut(s) 124, 466, 537
PkrI GCNGC 5 cut(s) 32, 40, 479, 631, 634
PleI GAGTC 2 cut(s) 913, 943
PpsI GAGTC 2 cut(s) 913, 943
PscI ACATGT 1 cut(s) 336
Psp1406I AACGTT 1 cut(s) 446
Psp6I CCWGG 2 cut(s) 300, 644
PspFI CCCAGC 1 cut(s) 653
PspGI CCWGG 2 cut(s) 300, 644
PspN4I GGNNCC 2 cut(s) 643, 961
PspPI GGNCC 1 cut(s) 987
PstI CTGCAG 1 cut(s) 35
PstNI CAGNNNCTG 2 cut(s) 599, 635
PvuII CAGCTG 1 cut(s) 632
RsaI GTAC 3 cut(s) 10, 458, 961
RsaNI GTAC 3 cut(s) 9, 457, 960
RseI CAYNNNNRTG 3 cut(s) 150, 850, 903
Rsr2I CGGWCCG 1 cut(s) 987
RsrII CGGWCCG 1 cut(s) 987
SaqAI TTAA 5 cut(s) 381, 408, 564, 1101, 1128
SatI GCNGC 5 cut(s) 31, 39, 478, 630, 633
Sau3AI GATC 4 cut(s) 253, 268, 489, 799
Sau96I GGNCC 1 cut(s) 987
ScaI AGTACT 1 cut(s) 10
SchI GAGTC 2 cut(s) 913, 943
ScrFI CCNGG 4 cut(s) 264, 281, 302, 646
SduI GDGCHC 3 cut(s) 279, 313, 590
SfaNI GCATC 2 cut(s) 366, 895
SfcI CTRYAG 2 cut(s) 31, 618
SinI GGWCC 1 cut(s) 987
SmiMI CAYNNNNRTG 3 cut(s) 150, 850, 903
SmlI CTYRAG 1 cut(s) 953
SmoI CTYRAG 1 cut(s) 953
Sse9I AATT 5 cut(s) 666, 685, 728, 1043, 1125
SseBI AGGCCT 1 cut(s) 624
SspI AATATT 1 cut(s) 826
SspMI CTAG 2 cut(s) 509, 1037
StuI AGGCCT 1 cut(s) 624
StyD4I CCNGG 4 cut(s) 262, 279, 300, 644
StyI CCWWGG 2 cut(s) 171, 194
TaaI ACNGT 3 cut(s) 317, 463, 595
TaiI ACGT 1 cut(s) 449
TaqI TCGA 4 cut(s) 395, 404, 689, 802
TaqII GACCGA 1 cut(s) 1004
TasI AATT 5 cut(s) 666, 685, 728, 1043, 1125
TatI WGTACW 2 cut(s) 8, 456
TfiI GAWTC 3 cut(s) 124, 466, 537
Tru1I TTAA 5 cut(s) 381, 408, 564, 1101, 1128
Tru9I TTAA 5 cut(s) 381, 408, 564, 1101, 1128
TscAI CASTG 6 cut(s) 295, 320, 466, 600, 823, 933
TseFI GTSAC 2 cut(s) 247, 341
TseI GCWGC 5 cut(s) 30, 38, 477, 629, 632
Tsp45I GTSAC 2 cut(s) 247, 341
TspDTI ATGAA 4 cut(s) 123, 458, 1034, 1036
TspGWI ACGGA 2 cut(s) 800, 1000
TspRI CASTG 6 cut(s) 295, 320, 466, 600, 823, 933
VpaK11BI GGWCC 1 cut(s) 987
XapI RAATTY 3 cut(s) 685, 728, 1043
XbaI TCTAGA 1 cut(s) 508
XceI RCATGY 2 cut(s) 340, 1099
XmiI GTMKAC 1 cut(s) 156
XmnI GAANNNNTTC 1 cut(s) 1113
XspI CTAG 2 cut(s) 509, 1037
ZrmI AGTACT 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.