Prupe.8G207400_v2.0.a1

alcohol dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0000815
Physical Location & Seq
Forward (+)
19370303 .. 19374893
4591 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G207400.1

Sequence Viewer

Length: 1239 bp
ATGCATTCGAGGTTTAGAACTTTGGTGCACAATGACCATTTCTTAAAGCTCCTAAAAAACCAAAAATTTCGCTTCATCAGCCCCGAACTCTACGACACTTCGGCTACCGCTGGCCGTGTGATCACCAGCAAAGCGGCGGTCGCATGGGAAGCAGGAAAGCCATTGGTGATAGAGAAGGTAGAGGTTGCTCCACCCCAGGCTAATGAAGTGAGGGTCAAAATCAAATATACCTCACTTTGTCACACTGATGTCCACTTCTGGGAAGCCAAGGGCCAGACTGGTCTCTTCCCCCGAATATTCGGTCATGAAGCAGCTGGAGTCGTTGAGAGTGTTGGGGACGGCGTTAAAAACCTCAAGCCTGGGGATCATGTACTTCCAGTTTTCACAGGGGAGTGTGGGGATTGCGTGCATTGCAAGTCTGAGGAAAGCAATATGTGTGACCTGCTGAGAATCAATACAGATAGAGGAGTTATGATTGGTGATGGGAAACAAAGGTTTTCCAAGAATGGGACTCCTATTAATCACTTTCTTGGCACATCTACCTTCAGTGAATATACAGTCATCCATGAAGGCTGCCTTGCCAAGATCGATCCGTCAGCACCGCTAGACAAAGTCTGCATCCTCAGTTGTGGTGTCGCAACAGGATTAGGTGCTACTCTGAAAGTGGCAAAACCAAAGAAGGGATCTTCAGTTGCTGTCTTTGGACTTGGGGCTGTTGGCCTGGCAGCTGCTGAAGGTGCAAGAATTTCTGGGGCATCAAGGATTATTGGGGTTGACTTGAATCCCAAGAGGCTTGAGGAAGCCAAGAACTTTGGAGTAAATGAGTTCGTGAACCCAAGGGACCATATCAAGCCAGTGCAACAGATTATTGCTGAGATGACCAACGGTGGAGTCGACAGAAGCATAGAGTGCACCGGCAACATTAATTCGATTATCTCCGCTTTTGAATGTGTTCATGATGGTTGGGGTGTGGCCGTTCTTGTAGGAGTGCCAAGCCCAGATGCAATCTTCAAGACTAACCCCTTCAATTTCCTTGATGAAAGAACCCTTAAGGGAACCTCTTTCGGGAACTACAAACCTCGGACTGACCTTCCCTCTGTTGTGGACATGTATATGAACAAAAAACTTGAAGTAGAGAAGTTTATAACTCATCGAGTTCCTTTCTCACAGATCAACAAAGCCTTTGACTACATGTTGAGGGGGGAAGGCTTGCGGTGCATCATTAGCATGGAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00071 ko00350 ko00592 ko01100 ko01110 ko01120 map00010 map00071 map00350 map00592 map01100 map01110 map01120
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

413

Amino Acids

44.97

Weight (kDa)

8.22

Isoelectric Point (pI)

32.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1145
Acc36I ACCTGC 1 cut(s) 450
AccI GTMKAC 1 cut(s) 894
AciI CCGC 6 cut(s) 108, 134, 137, 602, 939, 1213
AclWI GGATC 3 cut(s) 372, 584, 691
AcoI YGGCCR 2 cut(s) 112, 972
AcsI RAATTY 2 cut(s) 65, 744
AcuI CTGAAG 3 cut(s) 529, 672, 753
AfaI GTAC 1 cut(s) 372
AfiI CCNNNNNNNGG 4 cut(s) 259, 507, 680, 1065
AflII CTTAAG 1 cut(s) 1049
AflIII ACRYGT 2 cut(s) 1107, 1191
AgsI TTSAA 5 cut(s) 781, 947, 1012, 1027, 1130
AjnI CCWGG 3 cut(s) 195, 358, 720
AluBI AGCT 3 cut(s) 49, 314, 728
AluI AGCT 3 cut(s) 49, 314, 728
Alw21I GWGCWC 2 cut(s) 30, 914
Alw26I GTCTC 1 cut(s) 287
Alw44I GTGCAC 2 cut(s) 26, 910
AlwI GGATC 3 cut(s) 372, 584, 691
AlwNI CAGNNNCTG 2 cut(s) 695, 731
AoxI GGCC 4 cut(s) 112, 271, 718, 972
ApaLI GTGCAC 2 cut(s) 26, 910
ApeKI GCWGC 4 cut(s) 311, 573, 725, 728
ApoI RAATTY 2 cut(s) 65, 744
ArsI GACNNNNNNTTYG 4 cut(s) 123, 155, 286, 318
AseI ATTAAT 2 cut(s) 519, 924
Asp700I GAANNNNTTC 1 cut(s) 951
AspS9I GGNCC 2 cut(s) 271, 841
AsuHPI GGTGA 3 cut(s) 115, 178, 491
AvaII GGWCC 1 cut(s) 841
BaeGI GKGCMC 2 cut(s) 30, 914
Bbv12I GWGCWC 2 cut(s) 30, 914
BbvI GCAGC 4 cut(s) 323, 560, 715, 737
BccI CCATC 2 cut(s) 476, 953
BceAI ACGGC 3 cut(s) 99, 355, 959
BciT130I CCWGG 3 cut(s) 197, 360, 722
BclI TGATCA 1 cut(s) 120
BcoDI GTCTC 1 cut(s) 287
BfaI CTAG 1 cut(s) 605
BfrI CTTAAG 1 cut(s) 1049
BfuAI ACCTGC 1 cut(s) 450
BisI GCNGC 5 cut(s) 135, 312, 574, 726, 729
BlsI GCNGC 5 cut(s) 136, 313, 575, 727, 730
Bme1390I CCNGG 3 cut(s) 197, 360, 722
Bme18I GGWCC 1 cut(s) 841
BmgT120I GGNCC 2 cut(s) 271, 841
BmiI GGNNCC 2 cut(s) 842, 1057
BmrFI CCNGG 3 cut(s) 197, 360, 722
BmsI GCATC 4 cut(s) 627, 764, 991, 1227
BpmI CTGGAG 1 cut(s) 336
BpuEI CTTGAG 2 cut(s) 338, 815
Bsa29I ATCGAT 1 cut(s) 588
BsaI GGTCTC 1 cut(s) 287
BsaJI CCNNGG 5 cut(s) 195, 267, 359, 836, 1079
Bsc4I CCNNNNNNNGG 4 cut(s) 259, 507, 680, 1065
Bse118I RCCGGY 1 cut(s) 914
Bse1I ACTGG 3 cut(s) 283, 377, 854
Bse3DI GCAATG 1 cut(s) 409
BseBI CCWGG 3 cut(s) 197, 360, 722
BseCI ATCGAT 1 cut(s) 588
BseDI CCNNGG 5 cut(s) 195, 267, 359, 836, 1079
BseGI GGATG 2 cut(s) 561, 618
BseLI CCNNNNNNNGG 4 cut(s) 259, 507, 680, 1065
BseMI GCAATG 1 cut(s) 409
BseMII CTCAG 4 cut(s) 411, 437, 637, 864
BseNI ACTGG 3 cut(s) 283, 377, 854
BseRI GAGGAG 1 cut(s) 480
BseSI GKGCMC 2 cut(s) 30, 914
BseXI GCAGC 4 cut(s) 323, 560, 715, 737
Bsh1285I CGRYCG 1 cut(s) 141
BshFI GGCC 4 cut(s) 114, 273, 720, 974
BshVI ATCGAT 1 cut(s) 588
BsiEI CGRYCG 1 cut(s) 141
BsiHKAI GWGCWC 2 cut(s) 30, 914
BsiSI CCGG 1 cut(s) 915
BslFI GGGAC 3 cut(s) 350, 523, 854
BslI CCNNNNNNNGG 4 cut(s) 259, 507, 680, 1065
BsmAI GTCTC 1 cut(s) 287
BsmFI GGGAC 3 cut(s) 350, 523, 854
BsmI GAATGC 1 cut(s) 4
BsnI GGCC 4 cut(s) 114, 273, 720, 974
Bso31I GGTCTC 1 cut(s) 287
Bsp1286I GDGCHC 2 cut(s) 30, 914
Bsp143I GATC 6 cut(s) 120, 364, 585, 589, 683, 1170
BspACI CCGC 6 cut(s) 108, 134, 137, 602, 939, 1213
BspANI GGCC 4 cut(s) 114, 273, 720, 974
BspCNI CTCAG 4 cut(s) 412, 438, 636, 865
BspDI ATCGAT 1 cut(s) 588
BspHI TCATGA 2 cut(s) 304, 955
BspLI GGNNCC 2 cut(s) 842, 1057
BspMI ACCTGC 1 cut(s) 450
BspPI GGATC 3 cut(s) 372, 584, 691
BspTI CTTAAG 1 cut(s) 1049
BspTNI GGTCTC 1 cut(s) 287
BsrDI GCAATG 1 cut(s) 409
BsrFI RCCGGY 1 cut(s) 914
BsrI ACTGG 3 cut(s) 283, 377, 854
BssAI RCCGGY 1 cut(s) 914
BssECI CCNNGG 5 cut(s) 195, 267, 359, 836, 1079
BssMI GATC 6 cut(s) 120, 364, 585, 589, 683, 1170
BssT1I CCWWGG 2 cut(s) 267, 836
Bst2UI CCWGG 3 cut(s) 197, 360, 722
Bst4CI ACNGT 2 cut(s) 559, 887
Bst6I CTCTTC 2 cut(s) 290, 1227
BstAFI CTTAAG 1 cut(s) 1049
BstAPI GCANNNNNTGC 1 cut(s) 909
BstC8I GCNNGC 3 cut(s) 112, 407, 1211
BstDEI CTNAG 4 cut(s) 420, 446, 623, 873
BstF5I GGATG 2 cut(s) 561, 618
BstKTI GATC 6 cut(s) 123, 367, 588, 592, 686, 1173
BstMAI GTCTC 1 cut(s) 287
BstMBI GATC 6 cut(s) 120, 364, 585, 589, 683, 1170
BstMCI CGRYCG 1 cut(s) 141
BstMWI GCNNNNNNNGC 8 cut(s) 78, 140, 149, 411, 737, 909, 1215, 1224
BstNI CCWGG 3 cut(s) 197, 360, 722
BstNSI RCATGY 2 cut(s) 1111, 1195
BstSCI CCNGG 3 cut(s) 195, 358, 720
BstSLI GKGCMC 2 cut(s) 30, 914
BstV1I GCAGC 4 cut(s) 323, 560, 715, 737
BstX2I RGATCY 1 cut(s) 683
BstYI RGATCY 1 cut(s) 683
Bsu15I ATCGAT 1 cut(s) 588
BsuRI GGCC 4 cut(s) 114, 273, 720, 974
BsuTUI ATCGAT 1 cut(s) 588
BtsCI GGATG 2 cut(s) 561, 618
BtsIMutI CAGTG 3 cut(s) 243, 553, 861
BveI ACCTGC 1 cut(s) 450
Cac8I GCNNGC 3 cut(s) 112, 407, 1211
CaiI CAGNNNCTG 2 cut(s) 695, 731
CciI TCATGA 2 cut(s) 304, 955
Cfr10I RCCGGY 1 cut(s) 914
Cfr13I GGNCC 2 cut(s) 271, 841
ClaI ATCGAT 1 cut(s) 588
Csp6I GTAC 1 cut(s) 371
CviAII CATG 8 cut(s) 144, 305, 368, 566, 956, 1108, 1192, 1228
CviQI GTAC 1 cut(s) 371
DdeI CTNAG 4 cut(s) 420, 446, 623, 873
DpnI GATC 6 cut(s) 122, 366, 587, 591, 685, 1172
DpnII GATC 6 cut(s) 120, 364, 585, 589, 683, 1170
EaeI YGGCCR 2 cut(s) 112, 972
Eam1104I CTCTTC 2 cut(s) 290, 1227
EarI CTCTTC 2 cut(s) 290, 1227
Eco130I CCWWGG 2 cut(s) 267, 836
Eco31I GGTCTC 1 cut(s) 287
Eco47I GGWCC 1 cut(s) 841
Eco57I CTGAAG 3 cut(s) 529, 672, 753
EcoRII CCWGG 3 cut(s) 195, 358, 720
EcoT14I CCWWGG 2 cut(s) 267, 836
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 267, 836
FaeI CATG 8 cut(s) 147, 308, 371, 569, 959, 1111, 1195, 1231
FalI AAGNNNNNCTT 2 cut(s) 561, 593
FaqI GGGAC 3 cut(s) 350, 523, 854
FatI CATG 8 cut(s) 143, 304, 367, 565, 955, 1107, 1191, 1227
FbaI TGATCA 1 cut(s) 120
FblI GTMKAC 1 cut(s) 894
Fnu4HI GCNGC 5 cut(s) 135, 312, 574, 726, 729
FokI GGATG 2 cut(s) 548, 605
Fsp4HI GCNGC 5 cut(s) 135, 312, 574, 726, 729
FspBI CTAG 1 cut(s) 605
GluI GCNGC 5 cut(s) 135, 312, 574, 726, 729
GsuI CTGGAG 1 cut(s) 336
HaeIII GGCC 4 cut(s) 114, 273, 720, 974
HapII CCGG 1 cut(s) 915
Hin1II CATG 8 cut(s) 147, 308, 371, 569, 959, 1111, 1195, 1231
HincII GTYRAC 2 cut(s) 775, 895
HindII GTYRAC 2 cut(s) 775, 895
HinfI GANTC 5 cut(s) 318, 450, 511, 781, 891
HpaII CCGG 1 cut(s) 915
HphI GGTGA 3 cut(s) 115, 178, 491
Hpy166II GTNNAC 7 cut(s) 28, 253, 775, 832, 895, 912, 1105
Hpy188I TCNGA 3 cut(s) 421, 660, 1083
Hpy188III TCNNGA 5 cut(s) 305, 829, 956, 1012, 1066
Hpy8I GTNNAC 7 cut(s) 28, 253, 775, 832, 895, 912, 1105
HpyAV CCTTC 8 cut(s) 169, 553, 563, 673, 728, 1033, 1100, 1199
HpyCH4III ACNGT 2 cut(s) 559, 887
HpyF10VI GCNNNNNNNGC 8 cut(s) 78, 140, 149, 411, 737, 909, 1215, 1224
HpyF3I CTNAG 4 cut(s) 420, 446, 623, 873
Hsp92II CATG 8 cut(s) 147, 308, 371, 569, 959, 1111, 1195, 1231
Ksp22I TGATCA 1 cut(s) 120
Kzo9I GATC 6 cut(s) 120, 364, 585, 589, 683, 1170
LmnI GCTCC 2 cut(s) 54, 193
Lsp1109I GCAGC 4 cut(s) 323, 560, 715, 737
LweI GCATC 4 cut(s) 627, 764, 991, 1227
MaeI CTAG 1 cut(s) 605
MaeIII GTNAC 2 cut(s) 239, 437
MalI GATC 6 cut(s) 122, 366, 587, 591, 685, 1172
MboI GATC 6 cut(s) 120, 364, 585, 589, 683, 1170
MboII GAAGA 3 cut(s) 277, 678, 1000
MflI RGATCY 1 cut(s) 683
MhlI GDGCHC 2 cut(s) 30, 914
MluCI AATT 4 cut(s) 65, 744, 925, 1027
MlyI GAGTC 3 cut(s) 327, 505, 900
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 951
MseI TTAA 5 cut(s) 44, 345, 519, 924, 1050
MslI CAYNNNNRTG 3 cut(s) 246, 1112, 1226
MspA1I CMGCKG 3 cut(s) 110, 314, 728
MspCI CTTAAG 1 cut(s) 1049
MspI CCGG 1 cut(s) 915
MspR9I CCNGG 3 cut(s) 197, 360, 722
Mva1269I GAATGC 1 cut(s) 4
MvaI CCWGG 3 cut(s) 197, 360, 722
MwoI GCNNNNNNNGC 8 cut(s) 78, 140, 149, 411, 737, 909, 1215, 1224
NdeII GATC 6 cut(s) 120, 364, 585, 589, 683, 1170
NlaIII CATG 8 cut(s) 147, 308, 371, 569, 959, 1111, 1195, 1231
NlaIV GGNNCC 2 cut(s) 842, 1057
NmuCI GTSAC 2 cut(s) 239, 437
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 1111, 1195
PagI TCATGA 2 cut(s) 304, 955
PciI ACATGT 2 cut(s) 1107, 1191
PcsI WCGNNNNNNNCGW 1 cut(s) 891
PctI GAATGC 1 cut(s) 4
PdmI GAANNNNTTC 1 cut(s) 951
PfeI GAWTC 2 cut(s) 450, 781
PflFI GACNNNGTC 1 cut(s) 611
PkrI GCNGC 5 cut(s) 136, 313, 575, 727, 730
PleI GAGTC 3 cut(s) 326, 505, 899
PpsI GAGTC 3 cut(s) 326, 505, 899
PscI ACATGT 2 cut(s) 1107, 1191
PshBI ATTAAT 2 cut(s) 519, 924
PsiI TTATAA 1 cut(s) 1145
Psp6I CCWGG 3 cut(s) 195, 358, 720
PspGI CCWGG 3 cut(s) 195, 358, 720
PspN4I GGNNCC 2 cut(s) 842, 1057
PspPI GGNCC 2 cut(s) 271, 841
PstNI CAGNNNCTG 2 cut(s) 695, 731
PsuI RGATCY 1 cut(s) 683
PsyI GACNNNGTC 1 cut(s) 611
PvuII CAGCTG 2 cut(s) 314, 728
RsaI GTAC 1 cut(s) 372
RsaNI GTAC 1 cut(s) 371
RseI CAYNNNNRTG 3 cut(s) 246, 1112, 1226
SalI GTCGAC 1 cut(s) 893
SaqAI TTAA 5 cut(s) 44, 345, 519, 924, 1050
SatI GCNGC 5 cut(s) 135, 312, 574, 726, 729
Sau3AI GATC 6 cut(s) 120, 364, 585, 589, 683, 1170
Sau96I GGNCC 2 cut(s) 271, 841
SchI GAGTC 3 cut(s) 327, 505, 900
ScrFI CCNGG 3 cut(s) 197, 360, 722
SduI GDGCHC 2 cut(s) 30, 914
SfaNI GCATC 4 cut(s) 627, 764, 991, 1227
SinI GGWCC 1 cut(s) 841
SmiMI CAYNNNNRTG 3 cut(s) 246, 1112, 1226
SmlI CTYRAG 3 cut(s) 353, 794, 1049
SmoI CTYRAG 3 cut(s) 353, 794, 1049
Sse9I AATT 4 cut(s) 65, 744, 925, 1027
SsiI CCGC 6 cut(s) 108, 134, 137, 602, 939, 1213
SspI AATATT 1 cut(s) 297
SspMI CTAG 1 cut(s) 605
StyD4I CCNGG 3 cut(s) 195, 358, 720
StyI CCWWGG 2 cut(s) 267, 836
TaaI ACNGT 2 cut(s) 559, 887
TaqI TCGA 5 cut(s) 8, 588, 894, 929, 1153
TaqII GACCGA 1 cut(s) 290
TasI AATT 4 cut(s) 65, 744, 925, 1027
TatI WGTACW 1 cut(s) 370
TauI GCSGC 1 cut(s) 137
TfiI GAWTC 2 cut(s) 450, 781
Tru1I TTAA 5 cut(s) 44, 345, 519, 924, 1050
Tru9I TTAA 5 cut(s) 44, 345, 519, 924, 1050
TscAI CASTG 3 cut(s) 250, 553, 861
TseFI GTSAC 2 cut(s) 239, 437
TseI GCWGC 4 cut(s) 311, 573, 725, 728
Tsp45I GTSAC 2 cut(s) 239, 437
TspDTI ATGAA 7 cut(s) 64, 219, 321, 582, 944, 1053, 1130
TspGWI ACGGA 1 cut(s) 582
TspRI CASTG 3 cut(s) 250, 553, 861
Tth111I GACNNNGTC 1 cut(s) 611
Vha464I CTTAAG 1 cut(s) 1049
VneI GTGCAC 2 cut(s) 26, 910
VpaK11BI GGWCC 1 cut(s) 841
VspI ATTAAT 2 cut(s) 519, 924
XapI RAATTY 2 cut(s) 65, 744
XceI RCATGY 2 cut(s) 1111, 1195
XmiI GTMKAC 1 cut(s) 894
XmnI GAANNNNTTC 1 cut(s) 951
XspI CTAG 1 cut(s) 605
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.