MD05G1180800.v1.1

alcohol dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000815
Physical Location & Seq
Forward (+)
30824039 .. 30826770
2732 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1180800.v1.1.491

Sequence Viewer

Length: 1248 bp
ATGCACAAAATGCATAAGAGTTTTAGAACATTGGTGCACAATGACCATTTCTTGAAGCTCCTAAATAACCAAAAATCTCGATTCCTCAGACCAGAACTTTACAGCACTTCGGCTCATGCTGGCCGTGCGATCATCAGCAAAGCGGCTGTTGCTTGGGAAGCAGGGAAGCCCTTGGTGATAGAGAAGGTGGAGGTTGCTCCACCGCAAGCCAGTGAAGTTAGGGTCCAAATCAAATACACCTCTCTTTGTCACACTGATGTCTACTTCTGGGAAGCCAAGGGCCAGACACCATTGTTCCCTAGGATTTTCGGACATGAAGCAGCTGGCGTCGTTGAGAGTGTTGGCGAAGGCGTTAAGAACCTCAAGCCGGGGGATCATGTGCTTCCAGTTTTCACTGGGGAATGTGGGGATTGCCCACATTGCAAGTCTGAGGAAAGCAACATGTGTGAGCTGCTGAGAATCAATTGCGATAGAGGAGTCATGATTGGTGATGGGAAAGCAAGGTTTTCGCAGAATGGGACTCCGATTAATCACTTTCTCGGCACATCCACATTTAGCGAGTACACAGTTATCCACGAAGGCTGCCTCGCCAAGATCGACCCAAAGGCACCGTTGGATAAAGTCTGCATCCTCAGTTGTGGTGTGTCAACCGGCTTAGGTGCCACTCTGAATGTAGCAAAACCAAAGAAGGGTTCTTCAGTTGCTATCTTTGGACTTGGGGCTGTTGGCCTCGCTGCTGCAGAAGGTGCAAGAATTTCTGGGGCATCAAGGATTATTGGGGTAGACTTGAATCCCAATAGATTTGAGCAAGCCAAGAACTTCGGAGTAAACGAGTTTGTGAACCCGAAAGACCATAACAAGCCAGTGCAGGAGGTTATCGCTGAGATGACGGATGGTGGAGTTGACAGAAGCTTAGAGTGCACTGGGAACATTAATTCGATGATCTCCGCTTTCGAATGTGTTCATGATGGGTGGGGTGTAGCGGTGCTAGTAGGAGTGCCAACCAAAGATGCAATCTTCAAGACAAACCCCATGAAAGTACTTGATGAAAGAACCCTCAAAGGAACATTTTTCGGCAACTACAAGCCTCGGAGCGACCTTCCCTCTCTCGTGGACATGTACATGAACAAGAAACTGGAAGTAGAGAAGTTTATAACGCATCGACTTCCTTTCTCGGAGATCAACAGAGCTTTTGACCTAATGTTGAAGGGGGAAGGCTTGCGGTGCATCATTAGCATGGAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00071 ko00350 ko00592 ko01100 ko01110 ko01120 map00010 map00071 map00350 map00592 map01100 map01110 map01120
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

416

Amino Acids

45.41

Weight (kDa)

7.94

Isoelectric Point (pI)

33.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 70 - 199 4.6e-22 Alcohol dehydrogenase GroES-like domain
ADH_zinc_N PF00107 241 - 373 1.1e-26 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1154
AccB1I GGYRCC 2 cut(s) 607, 659
AccI GTMKAC 2 cut(s) 261, 783
AciI CCGC 5 cut(s) 143, 203, 948, 983, 1222
AclWI GGATC 1 cut(s) 381
AcoI YGGCCR 1 cut(s) 121
AcsI RAATTY 1 cut(s) 753
AcuI CTGAAG 1 cut(s) 681
AcyI GRCGYC 1 cut(s) 327
AfaI GTAC 3 cut(s) 563, 1041, 1121
AfiI CCNNNNNNNGG 2 cut(s) 367, 689
AflIII ACRYGT 2 cut(s) 441, 1116
AgsI TTSAA 4 cut(s) 55, 790, 1021, 1207
AjuI GAANNNNNNNTTGG 2 cut(s) 676, 708
AluBI AGCT 5 cut(s) 58, 323, 451, 912, 1190
AluI AGCT 5 cut(s) 58, 323, 451, 912, 1190
Alw21I GWGCWC 2 cut(s) 39, 923
Alw44I GTGCAC 2 cut(s) 35, 919
AlwI GGATC 1 cut(s) 381
AoxI GGCC 3 cut(s) 121, 280, 727
ApaLI GTGCAC 2 cut(s) 35, 919
ApeKI GCWGC 5 cut(s) 320, 451, 582, 734, 737
ApoI RAATTY 1 cut(s) 753
AseI ATTAAT 2 cut(s) 528, 933
Asp700I GAANNNNTTC 1 cut(s) 960
AspA2I CCTAGG 1 cut(s) 299
AspS9I GGNCC 2 cut(s) 223, 280
AsuC2I CCSGG 1 cut(s) 369
AsuHPI GGTGA 2 cut(s) 187, 500
AsuII TTCGAA 1 cut(s) 954
AvaII GGWCC 1 cut(s) 223
AvrII CCTAGG 1 cut(s) 299
BaeGI GKGCMC 2 cut(s) 39, 923
BanI GGYRCC 2 cut(s) 607, 659
BauI CACGAG 1 cut(s) 1109
Bbv12I GWGCWC 2 cut(s) 39, 923
BbvI GCAGC 5 cut(s) 332, 438, 569, 721, 724
BccI CCATC 3 cut(s) 485, 887, 962
BceAI ACGGC 1 cut(s) 108
BcgI CGANNNNNNTGC 2 cut(s) 489, 523
BcnI CCSGG 1 cut(s) 369
BfaI CTAG 2 cut(s) 300, 989
BfmI CTRYAG 1 cut(s) 738
BisI GCNGC 6 cut(s) 144, 321, 452, 583, 735, 738
BlnI CCTAGG 1 cut(s) 299
BlsI GCNGC 6 cut(s) 145, 322, 453, 584, 736, 739
BmcAI AGTACT 1 cut(s) 1041
Bme1390I CCNGG 1 cut(s) 369
Bme18I GGWCC 1 cut(s) 223
BmgT120I GGNCC 2 cut(s) 223, 280
BmiI GGNNCC 3 cut(s) 224, 609, 661
BmrFI CCNGG 1 cut(s) 369
BmrI ACTGGG 2 cut(s) 405, 933
BmsI GCATC 5 cut(s) 636, 773, 1000, 1168, 1236
BmuI ACTGGG 2 cut(s) 405, 933
Bpu10I CCTNAGC 1 cut(s) 655
Bpu14I TTCGAA 1 cut(s) 954
BpuEI CTTGAG 1 cut(s) 347
BpuMI CCSGG 1 cut(s) 369
BsaHI GRCGYC 1 cut(s) 327
BsaJI CCNNGG 5 cut(s) 171, 276, 299, 368, 1088
Bsc4I CCNNNNNNNGG 2 cut(s) 367, 689
Bse118I RCCGGY 1 cut(s) 650
Bse1I ACTGG 6 cut(s) 210, 386, 400, 863, 928, 1140
Bse3DI GCAATG 1 cut(s) 418
BseDI CCNNGG 5 cut(s) 171, 276, 299, 368, 1088
BseGI GGATG 3 cut(s) 545, 627, 898
BseLI CCNNNNNNNGG 2 cut(s) 367, 689
BseMI GCAATG 1 cut(s) 418
BseMII CTCAG 5 cut(s) 100, 420, 446, 646, 873
BseNI ACTGG 6 cut(s) 210, 386, 400, 863, 928, 1140
BseRI GAGGAG 1 cut(s) 489
BseSI GKGCMC 2 cut(s) 39, 923
BseXI GCAGC 5 cut(s) 332, 438, 569, 721, 724
BsgI GTGCAG 1 cut(s) 887
BshFI GGCC 3 cut(s) 123, 282, 729
BshNI GGYRCC 2 cut(s) 607, 659
BsiHKAI GWGCWC 2 cut(s) 39, 923
BsiSI CCGG 2 cut(s) 368, 651
BslFI GGGAC 1 cut(s) 532
BslI CCNNNNNNNGG 2 cut(s) 367, 689
BsmFI GGGAC 1 cut(s) 532
BsnI GGCC 3 cut(s) 123, 282, 729
Bsp119I TTCGAA 1 cut(s) 954
Bsp1286I GDGCHC 2 cut(s) 39, 923
Bsp1407I TGTACA 1 cut(s) 1119
Bsp143I GATC 5 cut(s) 129, 373, 594, 942, 1179
BspACI CCGC 5 cut(s) 143, 203, 948, 983, 1222
BspANI GGCC 3 cut(s) 123, 282, 729
BspCNI CTCAG 5 cut(s) 99, 421, 447, 645, 874
BspHI TCATGA 2 cut(s) 480, 964
BspLI GGNNCC 3 cut(s) 224, 609, 661
BspMAI CTGCAG 1 cut(s) 742
BspPI GGATC 1 cut(s) 381
BspT104I TTCGAA 1 cut(s) 954
BspT107I GGYRCC 2 cut(s) 607, 659
BsrDI GCAATG 1 cut(s) 418
BsrFI RCCGGY 1 cut(s) 650
BsrGI TGTACA 1 cut(s) 1119
BsrI ACTGG 6 cut(s) 210, 386, 400, 863, 928, 1140
BssAI RCCGGY 1 cut(s) 650
BssECI CCNNGG 5 cut(s) 171, 276, 299, 368, 1088
BssMI GATC 5 cut(s) 129, 373, 594, 942, 1179
BssNI GRCGYC 1 cut(s) 327
BssSI CACGAG 1 cut(s) 1109
BssT1I CCWWGG 3 cut(s) 171, 276, 299
Bst2BI CACGAG 1 cut(s) 1109
Bst4CI ACNGT 2 cut(s) 568, 612
Bst6I CTCTTC 1 cut(s) 1236
BstACI GRCGYC 1 cut(s) 327
BstAPI GCANNNNNTGC 2 cut(s) 10, 746
BstAUI TGTACA 1 cut(s) 1119
BstBI TTCGAA 1 cut(s) 954
BstC8I GCNNGC 5 cut(s) 121, 207, 325, 810, 1220
BstDEI CTNAG 7 cut(s) 86, 429, 455, 632, 655, 882, 913
BstF5I GGATG 3 cut(s) 545, 627, 898
BstKTI GATC 5 cut(s) 132, 376, 597, 945, 1182
BstMBI GATC 5 cut(s) 129, 373, 594, 942, 1179
BstMWI GCNNNNNNNGC 9 cut(s) 10, 125, 149, 158, 420, 746, 918, 1224, 1233
BstNSI RCATGY 2 cut(s) 445, 1120
BstSCI CCNGG 1 cut(s) 367
BstSFI CTRYAG 1 cut(s) 738
BstSLI GKGCMC 2 cut(s) 39, 923
BstV1I GCAGC 5 cut(s) 332, 438, 569, 721, 724
BsuRI GGCC 3 cut(s) 123, 282, 729
BtsCI GGATG 3 cut(s) 545, 627, 898
BtsIMutI CAGTG 5 cut(s) 217, 252, 393, 870, 921
Cac8I GCNNGC 5 cut(s) 121, 207, 325, 810, 1220
CciI TCATGA 2 cut(s) 480, 964
Cfr10I RCCGGY 1 cut(s) 650
Cfr13I GGNCC 2 cut(s) 223, 280
CseI GACGC 1 cut(s) 316
Csp6I GTAC 3 cut(s) 562, 1040, 1120
CviQI GTAC 3 cut(s) 562, 1040, 1120
DdeI CTNAG 7 cut(s) 86, 429, 455, 632, 655, 882, 913
DpnI GATC 5 cut(s) 131, 375, 596, 944, 1181
DpnII GATC 5 cut(s) 129, 373, 594, 942, 1179
EaeI YGGCCR 1 cut(s) 121
Eam1104I CTCTTC 1 cut(s) 1236
EarI CTCTTC 1 cut(s) 1236
Eco130I CCWWGG 3 cut(s) 171, 276, 299
Eco47I GGWCC 1 cut(s) 223
Eco57I CTGAAG 1 cut(s) 681
EcoT14I CCWWGG 3 cut(s) 171, 276, 299
EcoT22I ATGCAT 1 cut(s) 15
ErhI CCWWGG 3 cut(s) 171, 276, 299
FaqI GGGAC 1 cut(s) 532
FblI GTMKAC 2 cut(s) 261, 783
Fnu4HI GCNGC 6 cut(s) 144, 321, 452, 583, 735, 738
FokI GGATG 3 cut(s) 532, 614, 905
Fsp4HI GCNGC 6 cut(s) 144, 321, 452, 583, 735, 738
FspBI CTAG 2 cut(s) 300, 989
GluI GCNGC 6 cut(s) 144, 321, 452, 583, 735, 738
HaeIII GGCC 3 cut(s) 123, 282, 729
HapII CCGG 2 cut(s) 368, 651
HgaI GACGC 1 cut(s) 316
Hin1I GRCGYC 1 cut(s) 327
HincII GTYRAC 2 cut(s) 648, 904
HindII GTYRAC 2 cut(s) 648, 904
HindIII AAGCTT 1 cut(s) 910
HinfI GANTC 5 cut(s) 81, 459, 477, 520, 790
HpaII CCGG 2 cut(s) 368, 651
HphI GGTGA 2 cut(s) 187, 500
Hpy188I TCNGA 8 cut(s) 89, 311, 430, 525, 669, 824, 1092, 1177
Hpy188III TCNNGA 5 cut(s) 52, 78, 481, 965, 1021
Hpy99I CGWCG 1 cut(s) 332
HpyAV CCTTC 8 cut(s) 178, 341, 572, 682, 737, 1109, 1201, 1208
HpyCH4III ACNGT 2 cut(s) 568, 612
HpyF10VI GCNNNNNNNGC 9 cut(s) 10, 125, 149, 158, 420, 746, 918, 1224, 1233
HpyF3I CTNAG 7 cut(s) 86, 429, 455, 632, 655, 882, 913
Hsp92I GRCGYC 1 cut(s) 327
Kzo9I GATC 5 cut(s) 129, 373, 594, 942, 1179
LmnI GCTCC 3 cut(s) 63, 202, 1092
Lsp1109I GCAGC 5 cut(s) 332, 438, 569, 721, 724
LweI GCATC 5 cut(s) 636, 773, 1000, 1168, 1236
MaeI CTAG 2 cut(s) 300, 989
MaeIII GTNAC 1 cut(s) 248
MalI GATC 5 cut(s) 131, 375, 596, 944, 1181
MboI GATC 5 cut(s) 129, 373, 594, 942, 1179
MboII GAAGA 2 cut(s) 687, 1009
MfeI CAATTG 1 cut(s) 463
MhlI GDGCHC 2 cut(s) 39, 923
MluCI AATT 3 cut(s) 463, 753, 934
MlyI GAGTC 2 cut(s) 486, 514
MmeI TCCRAC 1 cut(s) 594
Mph1103I ATGCAT 1 cut(s) 15
MroXI GAANNNNTTC 1 cut(s) 960
MseI TTAA 3 cut(s) 354, 528, 933
MslI CAYNNNNRTG 3 cut(s) 255, 1121, 1235
MspA1I CMGCKG 1 cut(s) 323
MspI CCGG 2 cut(s) 368, 651
MspR9I CCNGG 1 cut(s) 369
MunI CAATTG 1 cut(s) 463
MwoI GCNNNNNNNGC 9 cut(s) 10, 125, 149, 158, 420, 746, 918, 1224, 1233
NciI CCSGG 1 cut(s) 369
NdeII GATC 5 cut(s) 129, 373, 594, 942, 1179
NlaIV GGNNCC 3 cut(s) 224, 609, 661
NmeAIII GCCGAG 1 cut(s) 519
NmuCI GTSAC 1 cut(s) 248
NsiI ATGCAT 1 cut(s) 15
NspI RCATGY 2 cut(s) 445, 1120
NspV TTCGAA 1 cut(s) 954
PagI TCATGA 2 cut(s) 480, 964
PciI ACATGT 2 cut(s) 441, 1116
PcsI WCGNNNNNNNCGW 2 cut(s) 594, 828
PdmI GAANNNNTTC 1 cut(s) 960
PfeI GAWTC 3 cut(s) 81, 459, 790
PkrI GCNGC 6 cut(s) 145, 322, 453, 584, 736, 739
PleI GAGTC 2 cut(s) 485, 514
PpsI GAGTC 2 cut(s) 485, 514
PscI ACATGT 2 cut(s) 441, 1116
PshBI ATTAAT 2 cut(s) 528, 933
PsiI TTATAA 1 cut(s) 1154
PspN4I GGNNCC 3 cut(s) 224, 609, 661
PspPI GGNCC 2 cut(s) 223, 280
PstI CTGCAG 1 cut(s) 742
PvuII CAGCTG 1 cut(s) 323
RsaI GTAC 3 cut(s) 563, 1041, 1121
RsaNI GTAC 3 cut(s) 562, 1040, 1120
RseI CAYNNNNRTG 3 cut(s) 255, 1121, 1235
SaqAI TTAA 3 cut(s) 354, 528, 933
SatI GCNGC 6 cut(s) 144, 321, 452, 583, 735, 738
Sau3AI GATC 5 cut(s) 129, 373, 594, 942, 1179
Sau96I GGNCC 2 cut(s) 223, 280
ScaI AGTACT 1 cut(s) 1041
SchI GAGTC 2 cut(s) 486, 514
ScrFI CCNGG 1 cut(s) 369
SduI GDGCHC 2 cut(s) 39, 923
SfaNI GCATC 5 cut(s) 636, 773, 1000, 1168, 1236
SfcI CTRYAG 1 cut(s) 738
SfuI TTCGAA 1 cut(s) 954
SinI GGWCC 1 cut(s) 223
SmiMI CAYNNNNRTG 3 cut(s) 255, 1121, 1235
SmlI CTYRAG 1 cut(s) 362
SmoI CTYRAG 1 cut(s) 362
Sse9I AATT 3 cut(s) 463, 753, 934
SsiI CCGC 5 cut(s) 143, 203, 948, 983, 1222
SspMI CTAG 2 cut(s) 300, 989
StyD4I CCNGG 1 cut(s) 367
StyI CCWWGG 3 cut(s) 171, 276, 299
TaaI ACNGT 2 cut(s) 568, 612
TaqI TCGA 5 cut(s) 79, 597, 938, 954, 1162
TasI AATT 3 cut(s) 463, 753, 934
TatI WGTACW 3 cut(s) 561, 1039, 1119
TauI GCSGC 1 cut(s) 146
TfiI GAWTC 3 cut(s) 81, 459, 790
Tru1I TTAA 3 cut(s) 354, 528, 933
Tru9I TTAA 3 cut(s) 354, 528, 933
TscAI CASTG 5 cut(s) 217, 259, 400, 870, 928
TseFI GTSAC 1 cut(s) 248
TseI GCWGC 5 cut(s) 320, 451, 582, 734, 737
Tsp45I GTSAC 1 cut(s) 248
TspDTI ATGAA 5 cut(s) 330, 953, 1049, 1062, 1139
TspGWI ACGGA 1 cut(s) 905
TspRI CASTG 5 cut(s) 217, 259, 400, 870, 928
VneI GTGCAC 2 cut(s) 35, 919
VpaK11BI GGWCC 1 cut(s) 223
VspI ATTAAT 2 cut(s) 528, 933
XapI RAATTY 1 cut(s) 753
XceI RCATGY 2 cut(s) 445, 1120
XmaJI CCTAGG 1 cut(s) 299
XmiI GTMKAC 2 cut(s) 261, 783
XmnI GAANNNNTTC 1 cut(s) 960
XspI CTAG 2 cut(s) 300, 989
ZrmI AGTACT 1 cut(s) 1041
Zsp2I ATGCAT 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.