Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6D

Orthogroup ID

OG0000815

Physical Location & Seq

Genomic Coordinates Reverse (-)

38400189 .. 38403256

Gene Structure 3068 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6DG214400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1143 bp

ATGTCCAACACTGCTGGCAAGGTGATCAAGTGCAAAGCGGCGGTAGCCTGGGAAGCTGGGAAGCCGCTGGTTATTGAGGAAGTGGAGGTGGCACCACCCCAGAAACATGAAGTACGCTTGAAGATCCTCTTTACTGCTCTATGCCACACTGATGTATACTTCTGGGAAGCAAAGGGACAAAATCCACTGTTCCCTCGGATTTTTGGTCATGAAGCTGGAGGAATCGTGGAAAGCGTGGGTGAGGGTGTAACTGAACTCCAACCGGGAGACCATGTCCTCCCAGTTTTCACCGGAGAATGCAAGGAGTGTGCACATTGTAAGTCAGAGGAAAGCAACATGTGTGATCTCCTAAGGATTAATACCGACAGGGGTACTATGCTCAATGACGGCCAGACCCGGTTTTCCAAGGATGGAAAGCCAATTTATCACTTTGTTGGTACCTCTACATTCAGTGAATACACTGTTTCACATGTTGGTTCCGTTGCGAAGATCAACCCTGCAGCGCCTCTTGACAAAGTTTGTGTTCTTAGTTGCGGAATTTGTACAGGTTTTGGTGCAACTGTAAATGTTGCAAAGCCGAAAAAGGGTTCATCTGTTGCCATTTTTGGATTGGGTGCTGTTGGTCTTGCTGCTGCGGAAGGTGCAAGGGTTTCTAGGGCTTCAAGGATCATTGGAGTTGATCTGAACTCCAACCGATTTGAGGAAGCGAAGAAGTTTGGGGTGAGTGAATTTGTGAATCCAAAAGATCATAACAAACCTGTTCAGGAGGTGATTGCTGAGATGACCAATGGTGGAGTTGATAGGAGTGTTGAGTGCACTGGAAGCATCCAAGCTATGATCTCTGCCTTTGAATGTGTTCATGATGGTTGGGGAGTTGCTGTACTTGTTGGAGTGCCAAGCAAAGACGATGCATTCAAAACCCATCCGATGAACTTCTTGAATGAGAGGACTCTTAAGGGTACTTTCTATGGTAACTACAAGCCCCGCAGCGATCTACCTGGTGTTGTGGAGAAGTACATGAACAAGGAGATGGAATTGGACAAATTTATCACTCACTCTGTTCCTTTCTCTGAGATCAACAAGGCATTTGAGTACATGCTGGCAGGAGAGGGAATCCGGTGCATCGTCCGCATGGAGCATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0001101 GO:0001666 GO:0003674 GO:0003824 GO:0004022 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006950 GO:0006970 GO:0006979 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009410 GO:0009413 GO:0009414 GO:0009415 GO:0009628 GO:0009636 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009743 GO:0009744 GO:0010033 GO:0010035 GO:0010038 GO:0010243 GO:0014070 GO:0014074 GO:0016020 GO:0016491 GO:0016614 GO:0016616 GO:0031000 GO:0032355 GO:0033993 GO:0034285 GO:0036270 GO:0036293 GO:0042221 GO:0042493 GO:0042542 GO:0042802 GO:0042803 GO:0043279 GO:0044424 GO:0044444 GO:0044464 GO:0046677 GO:0046686 GO:0046983 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0055114 GO:0065007 GO:0070482 GO:0071944 GO:0080134 GO:0080135 GO:0097305 GO:1900037 GO:1900039 GO:1901698 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00010 ko00071 ko00350 ko00592 ko01100 ko01110 ko01120 map00010 map00071 map00350 map00592 map01100 map01110 map01120

Pfam Domains

Protein Families

ADH_N ADH_zinc_N

Protein Analysis

380

Amino Acids

41.32

Weight (kDa)

6.33

Isoelectric Point (pI)

32.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ADH_N	PF08240	35 - 164	2.5e-19	Alcohol dehydrogenase GroES-like domain
Glu_dehyd_C	PF16912	197 - 365	5.6e-06	Glucose dehydrogenase C-terminus
ADH_zinc_N	PF00107	206 - 337	2.5e-25	Zinc-binding dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000815)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G77120
fragaria_vesca	FvH4_2g14750 FvH4_2g14750 FvH4_2g14760 FvH4_2g14760
malus_domestica	MD05G1013200.v1.1 MD05G1013400.v1.1 MD05G1179800.v1.1 MD05G1180800.v1.1 MD08G1068100.v1.1 MD09G1211500.v1.1 MD10G1013800.v1.1 MD10G1013900.v1.1 MD10G1014200.v1.1 MD10G1014500.v1.1 MD11G1004800.v1.1
prunus_persica	Prupe.8G018100_v2.0.a1 Prupe.8G018300_v2.0.a1 Prupe.8G018300_v2.0.a1 Prupe.8G018400_v2.0.a1 Prupe.8G018400_v2.0.a1 Prupe.8G018400_v2.0.a1 Prupe.8G018600_v2.0.a1 Prupe.8G207100_v2.0.a1 Prupe.8G207400_v2.0.a1
pyrus_communis	pycom05g00790 pycom05g16630 pycom10g00850 pycom10g00860 pycom10g00890
rosa_chinensis	RchiOBHm_Chr1g0331561 RchiOBHm_Chr6g0277181 RchiOBHm_Chr6g0277231 RchiOBHm_Chr6g0277261 RchiOBHm_Chr6g0277281 RchiOBHm_Chr6g0277311 RchiOBHm_Chr6g0277321
rosa_laevigata	RLG00000013310 RLG00000013311 RLG00000013312 RLG00000013313 RLG00000013319 RLG00000013325
rosa_multiflora	Rmu_co8243651.1_g000001 Rmu_sc0000030.1_g000028 Rmu_sc0000030.1_g000031 Rmu_sc0000030.1_g000035
rosa_roxburghii	Rroxscaffold_4G00319070 Rroxscaffold_7G00191450 Rroxscaffold_7G00191460 Rroxscaffold_7G00191470 Rroxscaffold_7G00191540
rosa_rugosa	Rorug01G0094000 Rorug01G0094100 Rorug06G0107000 Rorug06G0107100
rosa_samantha	Rh1BG090100 Rh1CG113500 Rh6BG221900 Rh6DG214200 Rh6DG214400 Rh6DG214700 Rh6DG214900
rosa_wichuraiana	Rw6G019010 Rw6G019020 Rw6G019030 Rw6G019040 Rw6G019080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	437
AccB1I	GGYRCC	2 cut(s)	91, 437
AccI	GTMKAC	1 cut(s)	156
AciI	CCGC	7 cut(s)	38, 41, 65, 534, 635, 985, 1129
AclWI	GGATC	2 cut(s)	118, 674
AcoI	YGGCCR	1 cut(s)	388
AcsI	RAATTY	3 cut(s)	537, 728, 1043
AfaI	GTAC	8 cut(s)	114, 373, 439, 544, 882, 961, 1016, 1094
AfiI	CCNNNNNNNGG	2 cut(s)	584, 700
AflII	CTTAAG	1 cut(s)	953
AflIII	ACRYGT	2 cut(s)	336, 469
AgsI	TTSAA	5 cut(s)	121, 663, 851, 916, 940
AjnI	CCWGG	2 cut(s)	47, 997
AluBI	AGCT	3 cut(s)	56, 215, 833
AluI	AGCT	3 cut(s)	56, 215, 833
Alw21I	GWGCWC	2 cut(s)	313, 818
Alw26I	GTCTC	1 cut(s)	261
Alw44I	GTGCAC	2 cut(s)	309, 814
AlwI	GGATC	2 cut(s)	118, 674
AoxI	GGCC	1 cut(s)	388
ApaLI	GTGCAC	2 cut(s)	309, 814
ApeKI	GCWGC	4 cut(s)	500, 629, 632, 987
ApoI	RAATTY	3 cut(s)	537, 728, 1043
AseI	ATTAAT	1 cut(s)	357
Asp700I	GAANNNNTTC	1 cut(s)	855
Asp718I	GGTACC	1 cut(s)	437
AspLEI	GCGC	1 cut(s)	505
AsuC2I	CCSGG	2 cut(s)	264, 397
AsuHPI	GGTGA	5 cut(s)	34, 251, 280, 733, 781
AxyI	CCTNAGG	1 cut(s)	350
BaeGI	GKGCMC	2 cut(s)	313, 818
BanI	GGYRCC	2 cut(s)	91, 437
Bbv12I	GWGCWC	2 cut(s)	313, 818
BbvI	GCAGC	4 cut(s)	512, 616, 619, 999
BccI	CCATC	4 cut(s)	404, 857, 930, 1024
BceAI	ACGGC	1 cut(s)	403
BciT130I	CCWGG	2 cut(s)	49, 999
BclI	TGATCA	1 cut(s)	24
BcnI	CCSGG	2 cut(s)	264, 397
BcoDI	GTCTC	1 cut(s)	261
BfaI	CTAG	1 cut(s)	654
BfmI	CTRYAG	1 cut(s)	498
BfoI	RGCGCY	1 cut(s)	506
BfrI	CTTAAG	1 cut(s)	953
BisI	GCNGC	6 cut(s)	39, 65, 501, 630, 633, 988
BlsI	GCNGC	6 cut(s)	40, 66, 502, 631, 634, 989
Bme1390I	CCNGG	4 cut(s)	49, 264, 397, 999
BmiI	GGNNCC	3 cut(s)	93, 439, 478
BmrFI	CCNGG	4 cut(s)	49, 264, 397, 999
BmrI	ACTGGG	1 cut(s)	275
BmsI	GCATC	3 cut(s)	834, 898, 1131
BmuI	ACTGGG	1 cut(s)	275
BpmI	CTGGAG	1 cut(s)	237
BpuMI	CCSGG	2 cut(s)	264, 397
BsaI	GGTCTC	1 cut(s)	261
BsaJI	CCNNGG	3 cut(s)	48, 194, 405
BsaWI	WCCGGW	2 cut(s)	290, 1116
BsaXI	ACNNNNNCTCC	6 cut(s)	210, 240, 258, 288, 864, 894
Bsc4I	CCNNNNNNNGG	2 cut(s)	584, 700
Bse1I	ACTGG	2 cut(s)	281, 823
Bse21I	CCTNAGG	1 cut(s)	350
BseBI	CCWGG	2 cut(s)	49, 999
BseDI	CCNNGG	3 cut(s)	48, 194, 405
BseGI	GGATG	3 cut(s)	415, 825, 922
BseLI	CCNNNNNNNGG	2 cut(s)	584, 700
BseMII	CTCAG	2 cut(s)	768, 1062
BseNI	ACTGG	2 cut(s)	281, 823
BseSI	GKGCMC	2 cut(s)	313, 818
BseXI	GCAGC	4 cut(s)	512, 616, 619, 999
BseYI	CCCAGC	1 cut(s)	56
BshFI	GGCC	1 cut(s)	390
BshNI	GGYRCC	2 cut(s)	91, 437
BsiHKAI	GWGCWC	2 cut(s)	313, 818
BsiSI	CCGG	4 cut(s)	263, 291, 397, 1117
BslFI	GGGAC	1 cut(s)	189
BslI	CCNNNNNNNGG	2 cut(s)	584, 700
BsmAI	GTCTC	1 cut(s)	261
BsmFI	GGGAC	1 cut(s)	189
BsmI	GAATGC	2 cut(s)	302, 911
BsnI	GGCC	1 cut(s)	390
Bso31I	GGTCTC	1 cut(s)	261
Bsp1286I	GDGCHC	2 cut(s)	313, 818
Bsp1407I	TGTACA	1 cut(s)	542
BspACI	CCGC	7 cut(s)	38, 41, 65, 534, 635, 985, 1129
BspANI	GGCC	1 cut(s)	390
BspCNI	CTCAG	2 cut(s)	769, 1063
BspHI	TCATGA	2 cut(s)	208, 859
BspLI	GGNNCC	3 cut(s)	93, 439, 478
BspMAI	CTGCAG	1 cut(s)	502
BspPI	GGATC	2 cut(s)	118, 674
BspT107I	GGYRCC	2 cut(s)	91, 437
BspTI	CTTAAG	1 cut(s)	953
BspTNI	GGTCTC	1 cut(s)	261
BsrGI	TGTACA	1 cut(s)	542
BsrI	ACTGG	2 cut(s)	281, 823
BssECI	CCNNGG	3 cut(s)	48, 194, 405
BssNAI	GTATAC	1 cut(s)	157
BssT1I	CCWWGG	1 cut(s)	405
Bst1107I	GTATAC	1 cut(s)	157
Bst2UI	CCWGG	2 cut(s)	49, 999
Bst4CI	ACNGT	3 cut(s)	189, 463, 562
BstAFI	CTTAAG	1 cut(s)	953
BstAUI	TGTACA	1 cut(s)	542
BstC8I	GCNNGC	2 cut(s)	16, 1101
BstDEI	CTNAG	4 cut(s)	350, 527, 777, 1071
BstF5I	GGATG	3 cut(s)	415, 825, 922
BstH2I	RGCGCY	1 cut(s)	506
BstHHI	GCGC	1 cut(s)	505
BstMAI	GTCTC	1 cut(s)	261
BstMWI	GCNNNNNNNGC	5 cut(s)	44, 53, 641, 822, 1128
BstNI	CCWGG	2 cut(s)	49, 999
BstNSI	RCATGY	3 cut(s)	340, 473, 1099
BstSCI	CCNGG	4 cut(s)	47, 262, 395, 997
BstSFI	CTRYAG	1 cut(s)	498
BstSLI	GKGCMC	2 cut(s)	313, 818
BstV1I	GCAGC	4 cut(s)	512, 616, 619, 999
BstX2I	RGATCY	1 cut(s)	123
BstYI	RGATCY	1 cut(s)	123
BstZ17I	GTATAC	1 cut(s)	157
Bsu36I	CCTNAGG	1 cut(s)	350
BsuRI	GGCC	1 cut(s)	390
BtsCI	GGATG	3 cut(s)	415, 825, 922
BtsI	GCAGTG	1 cut(s)	9
BtsIMutI	CAGTG	6 cut(s)	9, 147, 185, 457, 459, 816
Cac8I	GCNNGC	2 cut(s)	16, 1101
CciI	TCATGA	2 cut(s)	208, 859
CfoI	GCGC	1 cut(s)	505
CsiI	ACCWGGT	1 cut(s)	997
Csp6I	GTAC	8 cut(s)	113, 372, 438, 543, 881, 960, 1015, 1093
CviAII	CATG	9 cut(s)	107, 209, 272, 337, 470, 860, 1018, 1096, 1132
CviQI	GTAC	8 cut(s)	113, 372, 438, 543, 881, 960, 1015, 1093
DdeI	CTNAG	4 cut(s)	350, 527, 777, 1071
EaeI	YGGCCR	1 cut(s)	388
Eco130I	CCWWGG	1 cut(s)	405
Eco31I	GGTCTC	1 cut(s)	261
Eco81I	CCTNAGG	1 cut(s)	350
EcoRII	CCWGG	2 cut(s)	47, 997
EcoT14I	CCWWGG	1 cut(s)	405
EcoT22I	ATGCAT	1 cut(s)	913
ErhI	CCWWGG	1 cut(s)	405
FaeI	CATG	9 cut(s)	110, 212, 275, 340, 473, 863, 1021, 1099, 1135
FalI	AAGNNNNNCTT	2 cut(s)	113, 145
FaqI	GGGAC	1 cut(s)	189
FatI	CATG	9 cut(s)	106, 208, 271, 336, 469, 859, 1017, 1095, 1131
FauI	CCCGC	1 cut(s)	992
FbaI	TGATCA	1 cut(s)	24
FblI	GTMKAC	1 cut(s)	156
Fnu4HI	GCNGC	6 cut(s)	39, 65, 501, 630, 633, 988
FokI	GGATG	3 cut(s)	422, 812, 909
Fsp4HI	GCNGC	6 cut(s)	39, 65, 501, 630, 633, 988
FspBI	CTAG	1 cut(s)	654
GlaI	GCGC	1 cut(s)	504
GluI	GCNGC	6 cut(s)	39, 65, 501, 630, 633, 988
GsaI	CCCAGC	1 cut(s)	60
GsuI	CTGGAG	1 cut(s)	237
HaeII	RGCGCY	1 cut(s)	506
HaeIII	GGCC	1 cut(s)	390
HapII	CCGG	4 cut(s)	263, 291, 397, 1117
HhaI	GCGC	1 cut(s)	505
Hin1II	CATG	9 cut(s)	110, 212, 275, 340, 473, 863, 1021, 1099, 1135
Hin6I	GCGC	1 cut(s)	503
HinP1I	GCGC	1 cut(s)	503
HinfI	GANTC	4 cut(s)	222, 736, 949, 1113
HpaII	CCGG	4 cut(s)	263, 291, 397, 1117
HphI	GGTGA	5 cut(s)	34, 251, 280, 733, 781
Hpy166II	GTNNAC	3 cut(s)	157, 311, 816
Hpy188I	TCNGA	5 cut(s)	198, 325, 684, 927, 1072
Hpy188III	TCNNGA	5 cut(s)	209, 509, 764, 860, 937
Hpy8I	GTNNAC	3 cut(s)	157, 311, 816
HpyAV	CCTTC	1 cut(s)	632
HpyCH4III	ACNGT	3 cut(s)	189, 463, 562
HpyF10VI	GCNNNNNNNGC	5 cut(s)	44, 53, 641, 822, 1128
HpyF3I	CTNAG	4 cut(s)	350, 527, 777, 1071
Hsp92II	CATG	9 cut(s)	110, 212, 275, 340, 473, 863, 1021, 1099, 1135
HspAI	GCGC	1 cut(s)	503
KpnI	GGTACC	1 cut(s)	441
Ksp22I	TGATCA	1 cut(s)	24
LmnI	GCTCC	1 cut(s)	1135
Lsp1109I	GCAGC	4 cut(s)	512, 616, 619, 999
LweI	GCATC	3 cut(s)	834, 898, 1131
MabI	ACCWGGT	1 cut(s)	997
MaeI	CTAG	1 cut(s)	654
MaeIII	GTNAC	2 cut(s)	247, 971
MboII	GAAGA	3 cut(s)	133, 499, 721
MflI	RGATCY	1 cut(s)	123
MhlI	GDGCHC	2 cut(s)	313, 818
MluCI	AATT	5 cut(s)	420, 537, 728, 1034, 1043
MlyI	GAGTC	1 cut(s)	943
MmeI	TCCRAC	4 cut(s)	30, 283, 714, 868
Mph1103I	ATGCAT	1 cut(s)	913
MroXI	GAANNNNTTC	1 cut(s)	855
MseI	TTAA	2 cut(s)	357, 954
MslI	CAYNNNNRTG	1 cut(s)	150
MspA1I	CMGCKG	1 cut(s)	67
MspCI	CTTAAG	1 cut(s)	953
MspI	CCGG	4 cut(s)	263, 291, 397, 1117
MspR9I	CCNGG	4 cut(s)	49, 264, 397, 999
Mva1269I	GAATGC	2 cut(s)	302, 911
MvaI	CCWGG	2 cut(s)	49, 999
MwoI	GCNNNNNNNGC	5 cut(s)	44, 53, 641, 822, 1128
NciI	CCSGG	2 cut(s)	264, 397
NlaIII	CATG	9 cut(s)	110, 212, 275, 340, 473, 863, 1021, 1099, 1135
NlaIV	GGNNCC	3 cut(s)	93, 439, 478
NsiI	ATGCAT	1 cut(s)	913
NspI	RCATGY	3 cut(s)	340, 473, 1099
PagI	TCATGA	2 cut(s)	208, 859
PciI	ACATGT	2 cut(s)	336, 469
PcsI	WCGNNNNNNNCGW	1 cut(s)	231
PctI	GAATGC	2 cut(s)	302, 911
PdmI	GAANNNNTTC	1 cut(s)	855
PfeI	GAWTC	3 cut(s)	222, 736, 1113
PflFI	GACNNNGTC	1 cut(s)	272
PkrI	GCNGC	6 cut(s)	40, 66, 502, 631, 634, 989
PleI	GAGTC	1 cut(s)	943
PpsI	GAGTC	1 cut(s)	943
PscI	ACATGT	2 cut(s)	336, 469
PshBI	ATTAAT	1 cut(s)	357
Psp6I	CCWGG	2 cut(s)	47, 997
PspFI	CCCAGC	1 cut(s)	56
PspGI	CCWGG	2 cut(s)	47, 997
PspN4I	GGNNCC	3 cut(s)	93, 439, 478
PstI	CTGCAG	1 cut(s)	502
PsuI	RGATCY	1 cut(s)	123
PsyI	GACNNNGTC	1 cut(s)	272
RsaI	GTAC	8 cut(s)	114, 373, 439, 544, 882, 961, 1016, 1094
RsaNI	GTAC	8 cut(s)	113, 372, 438, 543, 881, 960, 1015, 1093
RseI	CAYNNNNRTG	1 cut(s)	150
SaqAI	TTAA	2 cut(s)	357, 954
SatI	GCNGC	6 cut(s)	39, 65, 501, 630, 633, 988
SchI	GAGTC	1 cut(s)	943
ScrFI	CCNGG	4 cut(s)	49, 264, 397, 999
SduI	GDGCHC	2 cut(s)	313, 818
SexAI	ACCWGGT	1 cut(s)	997
SfaNI	GCATC	3 cut(s)	834, 898, 1131
SfcI	CTRYAG	1 cut(s)	498
SmiMI	CAYNNNNRTG	1 cut(s)	150
SmlI	CTYRAG	1 cut(s)	953
SmoI	CTYRAG	1 cut(s)	953
Sse9I	AATT	5 cut(s)	420, 537, 728, 1034, 1043
SsiI	CCGC	7 cut(s)	38, 41, 65, 534, 635, 985, 1129
SspMI	CTAG	1 cut(s)	654
StyD4I	CCNGG	4 cut(s)	47, 262, 395, 997
StyI	CCWWGG	1 cut(s)	405
TaaI	ACNGT	3 cut(s)	189, 463, 562
TasI	AATT	5 cut(s)	420, 537, 728, 1034, 1043
TatI	WGTACW	4 cut(s)	542, 880, 1014, 1092
TauI	GCSGC	2 cut(s)	41, 67
TfiI	GAWTC	3 cut(s)	222, 736, 1113
Tru1I	TTAA	2 cut(s)	357, 954
Tru9I	TTAA	2 cut(s)	357, 954
TscAI	CASTG	6 cut(s)	16, 154, 192, 457, 466, 823
TseI	GCWGC	4 cut(s)	500, 629, 632, 987
TspDTI	ATGAA	6 cut(s)	123, 225, 579, 848, 944, 1034
TspGWI	ACGGA	1 cut(s)	469
TspRI	CASTG	6 cut(s)	16, 154, 192, 457, 466, 823
Tth111I	GACNNNGTC	1 cut(s)	272
Vha464I	CTTAAG	1 cut(s)	953
VneI	GTGCAC	2 cut(s)	309, 814
VspI	ATTAAT	1 cut(s)	357
XapI	RAATTY	3 cut(s)	537, 728, 1043
XceI	RCATGY	3 cut(s)	340, 473, 1099
XcmI	CCANNNNNNNNNTGG	1 cut(s)	607
XmiI	GTMKAC	1 cut(s)	156
XmnI	GAANNNNTTC	1 cut(s)	855
XspI	CTAG	1 cut(s)	654
Zsp2I	ATGCAT	1 cut(s)	913

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.