MD09G1073100.v1.1

PI-PLC X domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001750
Physical Location & Seq
Forward (+)
5068880 .. 5072015
3136 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1073100.v1.1.491

Sequence Viewer

Length: 1122 bp
ATGAAGACTCTGCATCACTTTTTGGTTGCAACTTGCAACATGAAGACTCTGCAGAACTTAGGGTTTACTCAGCAGAACCTCGTTTTGTTAGTCACGGTTTTCGCAGCGGTTTTAAGCATTGGACAGTGCAAGCTTTTGGACGAATGCTCGACGGACGCCGATTGTGAGGCCGGACTTCTCTGCGGGTCTTGCCCACAAGGCTTCTCAGGCACTAGATGTATAAGATCAGCCATTACAGATCAGTTCAAACTATTGAACAATTCTCTGCCGTTCAACAAGTATGCGTTTTTGACAACCCACAATGCCTTTGCAATTGACGGAGAGCCATCTCATACTGGAATTCCTCGCGTTACCTTCAACAATCAAGAAGAAACTGTCACTCAACAGCTCAGTGGTGGAGTTCGAGCCTTGATGCTGGATACGTATGATTTTAAGGACGATGTGTGGTTGTGCCATTCTTTTAAAGGAGAATGCCATGACTTTACTGCATTTGGACCAGCTATAGATACCCTGAAGGAAGTTGAAGCATTTTTATCAGCCAACCCATCAGAAATTGTGACATTGATATTAGAGGACTATGTGGAAGCTCCAAATGGATTGACAAAGGTCTTCGACGCTTCCGGGTTGAAGAAATATTGGTTTCCAGTGACAAGCATGCCCAAAACTGGCCAGGACTGGCCACTAGTTAGTGACATGGTTGCCAAGAACCAAAGGCTACTTGTGTTTACTTCAAAAAAAGCCAAGGAACAATCCGAAGGAATCGCTTACCAGTGGAACTACATGGTTGAAAACCAATATGGGGATGATGGAATGAAGGACGGTTGTTCAAATAGAGGAGAATCATCAACTCTTGATGACAAGACCAAATCTTTGGTTTTGGTGAATTATTTTGCTTCTGTTCCCATTAAGAAAGGCAGTTGCGTAGATAACTCTGGGAATCTGATTAACATGCTTTACACTTGTCATGGTGCTGCTGGCAACAGATGGGCTAACTTTGTTGCTAGAAGTGATGGAGGAGGATCGTTTCGAGCTTTAGACAATCTCAATGGGGAGCTCTTGTGTGGATGTAACGATGTACATGCATGTGTGCCAGGATCACCTCCAGGGTCTTGCAAGCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

374

Amino Acids

40.72

Weight (kDa)

5.4

Isoelectric Point (pI)

32.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC_cat PF26178 86 - 362 1.8e-137 PI-PLC-like catalytic domain
PI-PLC_X PF26146 117 - 303 7.6e-23 PI-PLC X
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 348
AciI CCGC 2 cut(s) 107, 183
AclWI GGATC 2 cut(s) 1027, 1102
AcoI YGGCCR 2 cut(s) 667, 677
AcsI RAATTY 1 cut(s) 339
AcuI CTGAAG 1 cut(s) 533
AcyI GRCGYC 1 cut(s) 156
AfaI GTAC 1 cut(s) 1077
AfiI CCNNNNNNNGG 2 cut(s) 665, 799
AgsI TTSAA 9 cut(s) 247, 256, 274, 358, 524, 628, 732, 788, 828
AhlI ACTAGT 1 cut(s) 682
AjnI CCWGG 3 cut(s) 669, 1090, 1102
AluBI AGCT 6 cut(s) 133, 388, 500, 587, 1031, 1054
AluI AGCT 6 cut(s) 133, 388, 500, 587, 1031, 1054
Alw21I GWGCWC 1 cut(s) 1056
AlwI GGATC 2 cut(s) 1027, 1102
AoxI GGCC 3 cut(s) 168, 667, 677
ApeKI GCWGC 2 cut(s) 104, 971
ApoI RAATTY 1 cut(s) 339
ArsI GACNNNNNNTTYG 2 cut(s) 853, 885
AspS9I GGNCC 1 cut(s) 494
AsuC2I CCSGG 1 cut(s) 622
AsuHPI GGTGA 2 cut(s) 892, 1089
AvaII GGWCC 1 cut(s) 494
BalI TGGCCA 2 cut(s) 669, 679
BanII GRGCYC 1 cut(s) 1056
BbsI GAAGAC 3 cut(s) 11, 50, 601
Bbv12I GWGCWC 1 cut(s) 1056
BbvI GCAGC 2 cut(s) 116, 958
BccI CCATC 5 cut(s) 334, 553, 800, 978, 1004
BceAI ACGGC 1 cut(s) 253
BcgI CGANNNNNNTGC 2 cut(s) 1061, 1095
BciT130I CCWGG 3 cut(s) 671, 1092, 1104
BciVI GTATCC 1 cut(s) 412
BcnI CCSGG 1 cut(s) 622
BcuI ACTAGT 1 cut(s) 682
BfaI CTAG 3 cut(s) 213, 683, 1002
BfmI CTRYAG 2 cut(s) 50, 501
BfuI GTATCC 1 cut(s) 412
BglI GCCNNNNNGGC 1 cut(s) 198
BisI GCNGC 2 cut(s) 105, 972
BlsI GCNGC 2 cut(s) 106, 973
Bme1390I CCNGG 4 cut(s) 622, 671, 1092, 1104
Bme18I GGWCC 1 cut(s) 494
BmgT120I GGNCC 1 cut(s) 494
BmrFI CCNGG 4 cut(s) 622, 671, 1092, 1104
BmsI GCATC 2 cut(s) 22, 402
BoxI GACNNNNGTC 1 cut(s) 605
BpiI GAAGAC 3 cut(s) 11, 50, 601
BpmI CTGGAG 1 cut(s) 1086
BpuMI CCSGG 1 cut(s) 622
BsaAI YACGTR 1 cut(s) 423
BsaHI GRCGYC 1 cut(s) 156
BsaJI CCNNGG 2 cut(s) 741, 1103
BsaXI ACNNNNNCTCC 2 cut(s) 1043, 1073
Bsc4I CCNNNNNNNGG 2 cut(s) 665, 799
Bse1I ACTGG 5 cut(s) 340, 644, 670, 680, 769
BseBI CCWGG 3 cut(s) 671, 1092, 1104
BseDI CCNNGG 2 cut(s) 741, 1103
BseGI GGATG 2 cut(s) 808, 1070
BseLI CCNNNNNNNGG 2 cut(s) 665, 799
BseMII CTCAG 3 cut(s) 83, 219, 403
BseNI ACTGG 5 cut(s) 340, 644, 670, 680, 769
BseRI GAGGAG 2 cut(s) 849, 1029
BseXI GCAGC 2 cut(s) 116, 958
Bsh1236I CGCG 1 cut(s) 348
BshFI GGCC 3 cut(s) 170, 669, 679
BsiHKAI GWGCWC 1 cut(s) 1056
BsiSI CCGG 2 cut(s) 171, 621
BslI CCNNNNNNNGG 2 cut(s) 665, 799
BsmI GAATGC 2 cut(s) 149, 476
BsnI GGCC 3 cut(s) 170, 669, 679
Bsp1286I GDGCHC 1 cut(s) 1056
Bsp1407I TGTACA 1 cut(s) 1075
Bsp143I GATC 4 cut(s) 224, 238, 1019, 1094
BspACI CCGC 2 cut(s) 107, 183
BspANI GGCC 3 cut(s) 170, 669, 679
BspCNI CTCAG 3 cut(s) 82, 218, 402
BspFNI CGCG 1 cut(s) 348
BspMAI CTGCAG 1 cut(s) 54
BspPI GGATC 2 cut(s) 1027, 1102
BsrGI TGTACA 1 cut(s) 1075
BsrI ACTGG 5 cut(s) 340, 644, 670, 680, 769
BssECI CCNNGG 2 cut(s) 741, 1103
BssMI GATC 4 cut(s) 224, 238, 1019, 1094
BssNI GRCGYC 1 cut(s) 156
BssT1I CCWWGG 1 cut(s) 741
Bst2UI CCWGG 3 cut(s) 671, 1092, 1104
Bst4CI ACNGT 4 cut(s) 97, 126, 376, 821
BstACI GRCGYC 1 cut(s) 156
BstAUI TGTACA 1 cut(s) 1075
BstBAI YACGTR 1 cut(s) 423
BstC8I GCNNGC 4 cut(s) 131, 656, 976, 1115
BstDEI CTNAG 4 cut(s) 58, 69, 205, 389
BstF5I GGATG 2 cut(s) 808, 1070
BstFNI CGCG 1 cut(s) 348
BstKTI GATC 4 cut(s) 227, 241, 1022, 1097
BstMBI GATC 4 cut(s) 224, 238, 1019, 1094
BstMWI GCNNNNNNNGC 3 cut(s) 189, 198, 207
BstNI CCWGG 3 cut(s) 671, 1092, 1104
BstNSI RCATGY 4 cut(s) 658, 952, 1082, 1086
BstPAI GACNNNNGTC 1 cut(s) 605
BstSCI CCNGG 4 cut(s) 620, 669, 1090, 1102
BstSFI CTRYAG 2 cut(s) 50, 501
BstSNI TACGTA 1 cut(s) 423
BstUI CGCG 1 cut(s) 348
BstV1I GCAGC 2 cut(s) 116, 958
BstV2I GAAGAC 3 cut(s) 11, 50, 601
BstXI CCANNNNNNTGG 1 cut(s) 871
BsuI GTATCC 1 cut(s) 412
BsuRI GGCC 3 cut(s) 170, 669, 679
BtsCI GGATG 2 cut(s) 808, 1070
BtsIMutI CAGTG 4 cut(s) 131, 397, 651, 776
Cac8I GCNNGC 4 cut(s) 131, 656, 976, 1115
Cfr13I GGNCC 1 cut(s) 494
CseI GACGC 2 cut(s) 164, 623
Csp6I GTAC 1 cut(s) 1076
CviAII CATG 9 cut(s) 40, 476, 655, 694, 781, 949, 965, 1079, 1083
CviQI GTAC 1 cut(s) 1076
DdeI CTNAG 4 cut(s) 58, 69, 205, 389
DpnI GATC 4 cut(s) 226, 240, 1021, 1096
DpnII GATC 4 cut(s) 224, 238, 1019, 1094
DraI TTTAAA 1 cut(s) 463
EaeI YGGCCR 2 cut(s) 667, 677
Ecl136II GAGCTC 1 cut(s) 1054
Eco105I TACGTA 1 cut(s) 423
Eco130I CCWWGG 1 cut(s) 741
Eco24I GRGCYC 1 cut(s) 1056
Eco47I GGWCC 1 cut(s) 494
Eco53kI GAGCTC 1 cut(s) 1054
Eco57I CTGAAG 1 cut(s) 533
EcoICRI GAGCTC 1 cut(s) 1054
EcoRI GAATTC 1 cut(s) 339
EcoRII CCWGG 3 cut(s) 669, 1090, 1102
EcoT14I CCWWGG 1 cut(s) 741
EcoT22I ATGCAT 1 cut(s) 1084
EcoT38I GRGCYC 1 cut(s) 1056
ErhI CCWWGG 1 cut(s) 741
FaeI CATG 9 cut(s) 43, 479, 658, 697, 784, 952, 968, 1082, 1086
FatI CATG 9 cut(s) 39, 475, 654, 693, 780, 948, 964, 1078, 1082
FauI CCCGC 1 cut(s) 176
Fnu4HI GCNGC 2 cut(s) 105, 972
FokI GGATG 2 cut(s) 815, 1077
FriOI GRGCYC 1 cut(s) 1056
Fsp4HI GCNGC 2 cut(s) 105, 972
FspBI CTAG 3 cut(s) 213, 683, 1002
GluI GCNGC 2 cut(s) 105, 972
GsuI CTGGAG 1 cut(s) 1086
HaeIII GGCC 3 cut(s) 170, 669, 679
HapII CCGG 2 cut(s) 171, 621
HgaI GACGC 2 cut(s) 164, 623
Hin1I GRCGYC 1 cut(s) 156
Hin1II CATG 9 cut(s) 43, 479, 658, 697, 784, 952, 968, 1082, 1086
HindIII AAGCTT 1 cut(s) 131
HinfI GANTC 5 cut(s) 7, 46, 759, 839, 937
HpaII CCGG 2 cut(s) 171, 621
HphI GGTGA 2 cut(s) 892, 1089
Hpy166II GTNNAC 2 cut(s) 66, 726
Hpy188I TCNGA 3 cut(s) 550, 754, 942
Hpy188III TCNNGA 2 cut(s) 365, 851
Hpy8I GTNNAC 2 cut(s) 66, 726
Hpy99I CGWCG 2 cut(s) 154, 617
HpyAV CCTTC 4 cut(s) 364, 508, 749, 808
HpyCH4III ACNGT 4 cut(s) 97, 126, 376, 821
HpyCH4IV ACGT 1 cut(s) 422
HpyCH4V TGCA 9 cut(s) 13, 29, 36, 52, 129, 311, 488, 1082, 1113
HpyF10VI GCNNNNNNNGC 3 cut(s) 189, 198, 207
HpyF3I CTNAG 4 cut(s) 58, 69, 205, 389
HpySE526I ACGT 1 cut(s) 422
Hsp92I GRCGYC 1 cut(s) 156
Hsp92II CATG 9 cut(s) 43, 479, 658, 697, 784, 952, 968, 1082, 1086
Kzo9I GATC 4 cut(s) 224, 238, 1019, 1094
LmnI GCTCC 2 cut(s) 592, 1051
Lsp1109I GCAGC 2 cut(s) 116, 958
LweI GCATC 2 cut(s) 22, 402
MaeI CTAG 3 cut(s) 213, 683, 1002
MaeII ACGT 1 cut(s) 422
MaeIII GTNAC 7 cut(s) 91, 349, 376, 556, 646, 689, 1067
MalI GATC 4 cut(s) 226, 240, 1021, 1096
MboI GATC 4 cut(s) 224, 238, 1019, 1094
MboII GAAGA 5 cut(s) 16, 55, 380, 601, 640
MfeI CAATTG 1 cut(s) 312
MhlI GDGCHC 1 cut(s) 1056
MlsI TGGCCA 2 cut(s) 669, 679
MluCI AATT 5 cut(s) 259, 312, 339, 552, 883
MluNI TGGCCA 2 cut(s) 669, 679
MlyI GAGTC 1 cut(s) 40
MnlI CCTC 8 cut(s) 89, 160, 354, 565, 827, 1007, 1010, 1110
Mox20I TGGCCA 2 cut(s) 669, 679
Mph1103I ATGCAT 1 cut(s) 1084
MscI TGGCCA 2 cut(s) 669, 679
MseI TTAA 5 cut(s) 113, 432, 462, 906, 945
MslI CAYNNNNRTG 1 cut(s) 1083
Msp20I TGGCCA 2 cut(s) 669, 679
MspA1I CMGCKG 1 cut(s) 107
MspI CCGG 2 cut(s) 171, 621
MspR9I CCNGG 4 cut(s) 622, 671, 1092, 1104
MunI CAATTG 1 cut(s) 312
Mva1269I GAATGC 2 cut(s) 149, 476
MvaI CCWGG 3 cut(s) 671, 1092, 1104
MvnI CGCG 1 cut(s) 348
MwoI GCNNNNNNNGC 3 cut(s) 189, 198, 207
NciI CCSGG 1 cut(s) 622
NdeII GATC 4 cut(s) 224, 238, 1019, 1094
NlaIII CATG 9 cut(s) 43, 479, 658, 697, 784, 952, 968, 1082, 1086
NmuCI GTSAC 5 cut(s) 91, 376, 556, 646, 689
NsiI ATGCAT 1 cut(s) 1084
NspI RCATGY 4 cut(s) 658, 952, 1082, 1086
PaeI GCATGC 1 cut(s) 658
PctI GAATGC 2 cut(s) 149, 476
PfeI GAWTC 3 cut(s) 759, 839, 937
PkrI GCNGC 2 cut(s) 106, 973
PleI GAGTC 1 cut(s) 40
PpsI GAGTC 1 cut(s) 40
Ppu21I YACGTR 1 cut(s) 423
PshAI GACNNNNGTC 1 cut(s) 605
Psp124BI GAGCTC 1 cut(s) 1056
Psp6I CCWGG 3 cut(s) 669, 1090, 1102
PspGI CCWGG 3 cut(s) 669, 1090, 1102
PspPI GGNCC 1 cut(s) 494
PstI CTGCAG 1 cut(s) 54
RsaI GTAC 1 cut(s) 1077
RsaNI GTAC 1 cut(s) 1076
RseI CAYNNNNRTG 1 cut(s) 1083
SacI GAGCTC 1 cut(s) 1056
SaqAI TTAA 5 cut(s) 113, 432, 462, 906, 945
SatI GCNGC 2 cut(s) 105, 972
Sau3AI GATC 4 cut(s) 224, 238, 1019, 1094
Sau96I GGNCC 1 cut(s) 494
SchI GAGTC 1 cut(s) 40
ScrFI CCNGG 4 cut(s) 622, 671, 1092, 1104
SduI GDGCHC 1 cut(s) 1056
SfaNI GCATC 2 cut(s) 22, 402
SfcI CTRYAG 2 cut(s) 50, 501
SinI GGWCC 1 cut(s) 494
SmiMI CAYNNNNRTG 1 cut(s) 1083
SnaBI TACGTA 1 cut(s) 423
SpeI ACTAGT 1 cut(s) 682
SphI GCATGC 1 cut(s) 658
Sse9I AATT 5 cut(s) 259, 312, 339, 552, 883
SsiI CCGC 2 cut(s) 107, 183
SspI AATATT 1 cut(s) 635
SspMI CTAG 3 cut(s) 213, 683, 1002
SstI GAGCTC 1 cut(s) 1056
StyD4I CCNGG 4 cut(s) 620, 669, 1090, 1102
StyI CCWWGG 1 cut(s) 741
TaaI ACNGT 4 cut(s) 97, 126, 376, 821
TaiI ACGT 1 cut(s) 425
TaqI TCGA 4 cut(s) 149, 403, 612, 1027
TasI AATT 5 cut(s) 259, 312, 339, 552, 883
TatI WGTACW 1 cut(s) 1075
TfiI GAWTC 3 cut(s) 759, 839, 937
Tru1I TTAA 5 cut(s) 113, 432, 462, 906, 945
Tru9I TTAA 5 cut(s) 113, 432, 462, 906, 945
TscAI CASTG 4 cut(s) 131, 397, 651, 776
TseFI GTSAC 5 cut(s) 91, 376, 556, 646, 689
TseI GCWGC 2 cut(s) 104, 971
Tsp45I GTSAC 5 cut(s) 91, 376, 556, 646, 689
TspDTI ATGAA 3 cut(s) 17, 56, 827
TspGWI ACGGA 2 cut(s) 167, 333
TspRI CASTG 4 cut(s) 131, 397, 651, 776
VpaK11BI GGWCC 1 cut(s) 494
XapI RAATTY 1 cut(s) 339
XceI RCATGY 4 cut(s) 658, 952, 1082, 1086
XspI CTAG 3 cut(s) 213, 683, 1002
Zsp2I ATGCAT 1 cut(s) 1084
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.