Rh1DG024300

PI-PLC X domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0001750
Physical Location & Seq
Forward (+)
3830099 .. 3832613
2515 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG024300.1

Sequence Viewer

Length: 1089 bp
ATGAAGACGACTCGGCATAGCTTCCTTCTGGTTCTAGTTCTGGTTATGGCTTTTGCTGTGTTTTCATGTGTCGTCATAGCTTGCTCCGATGGACAGTGCAAGCTCTTAGATCAGTGCTCGACGGACGGAGATTGTGAGGCGGGGCTTTACTGTTTCGGTTGCCCTTCGGAGTTTTCTGGCTCCAGATGTGTGAGATCAACCACCACCAACCAATTCCAGCTTTTGAATAATTCTCTACCATTCAACAAATATGCATTTTTGACAACCCACAATGCTTTTGCTATCGAAGGAGAGCCATCTCATACGGGAGTCCCTCGTGTTACCTTCACGAATCAAGAAGATACTGTCACTCAACAACTTAACAATGGAGTTAGAGCCCTAATGCTTGATACCTATGATTTTAAAGGAGATGTCTGGTTGTGCCATTCCTTCAAAGGAAAATGTCATGACTACACTGCATTTGAGCCAGCTATAGATACATTGAATGAAATCCAAGCATTTTTATCAGCAAACCCAGGAGAAATTGTGACATTGATATTAGAGGACTATGTTGAAGCTCCAAACGGATTGACAAATGTTTTCAAAGCTGCCGGATTGATGAAATACTGGTTTCCGGTATCAAACATGCCCAAAAGTGGTCAGGATTGGCCGTTGGTTAGCGATATGGTCACTAAGAACCAAAGGCTACTTGTATTCACTTCAAAACAAGAGAAGGAACAATCCGAAGGGATTGCATACCAGTGGAACTATATGGTTGAAAACCAGTATGGAAATGATGGAATGAAAGCGGGAAGCTGTTCAAATAGAGCTGAATCGTCGCCTCTAAATGACAAGACTAAATCATTGGTGTTGGTTAACTATTTTGGGTCAGTTCCCATTAAGCAGCTCTCATGTCAATTCAATTCTGAGGATTTGGTTAGCATGCTTAACACTTGCTATGGTGCTGCTAGAAACCGATGGGCGAATTTTGTGCGGTTGATTTTTACAAGAGGAGTGGAGGAGGGGGATCGTTTCAAGCTACAGACACTCTCAATGGAGAACTCATATGTGGATGTAACGATGTCCATGCATGTGTGCCAGGATCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

362

Amino Acids

40.56

Weight (kDa)

5.23

Isoelectric Point (pI)

35.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC_cat PF26178 75 - 333 4.5e-131 PI-PLC-like catalytic domain
PI-PLC_X PF26146 106 - 296 5.9e-24 PI-PLC X
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 140, 788, 973
AclWI GGATC 1 cut(s) 1014
AcoI YGGCCR 1 cut(s) 647
AcsI RAATTY 1 cut(s) 964
AfiI CCNNNNNNNGG 1 cut(s) 635
AjnI CCWGG 2 cut(s) 514, 1077
Alw21I GWGCWC 1 cut(s) 119
AlwI GGATC 1 cut(s) 1014
AoxI GGCC 1 cut(s) 647
ApeKI GCWGC 3 cut(s) 587, 883, 944
ApoI RAATTY 1 cut(s) 964
Asp700I GAANNNNTTC 1 cut(s) 796
BanII GRGCYC 1 cut(s) 379
BauI CACGAG 1 cut(s) 315
BbsI GAAGAC 1 cut(s) 11
Bbv12I GWGCWC 1 cut(s) 119
BbvI GCAGC 3 cut(s) 574, 895, 931
BccI CCATC 4 cut(s) 83, 304, 770, 951
BceAI ACGGC 1 cut(s) 634
BcgI CGANNNNNNTGC 6 cut(s) 713, 747, 952, 986, 1048, 1082
BciT130I CCWGG 2 cut(s) 516, 1079
BfaI CTAG 2 cut(s) 35, 948
BfmI CTRYAG 2 cut(s) 471, 1019
BisI GCNGC 3 cut(s) 588, 884, 945
BlsI GCNGC 3 cut(s) 589, 885, 946
Bme1390I CCNGG 2 cut(s) 516, 1079
BmiI GGNNCC 1 cut(s) 181
BmrFI CCNGG 2 cut(s) 516, 1079
BpiI GAAGAC 1 cut(s) 11
BpmI CTGGAG 1 cut(s) 166
BsaJI CCNNGG 1 cut(s) 514
BsaWI WCCGGW 1 cut(s) 613
BsaXI ACNNNNNCTCC 2 cut(s) 510, 540
Bsc4I CCNNNNNNNGG 1 cut(s) 635
Bse1I ACTGG 3 cut(s) 611, 739, 763
BseBI CCWGG 2 cut(s) 516, 1079
BseDI CCNNGG 1 cut(s) 514
BseGI GGATG 1 cut(s) 1057
BseLI CCNNNNNNNGG 1 cut(s) 635
BseMII CTCAG 1 cut(s) 897
BseNI ACTGG 3 cut(s) 611, 739, 763
BseRI GAGGAG 2 cut(s) 1005, 1013
BseXI GCAGC 3 cut(s) 574, 895, 931
BshFI GGCC 1 cut(s) 649
BsiHKAI GWGCWC 1 cut(s) 119
BsiSI CCGG 2 cut(s) 591, 614
BslFI GGGAC 1 cut(s) 296
BslI CCNNNNNNNGG 1 cut(s) 635
BsmFI GGGAC 1 cut(s) 296
BsnI GGCC 1 cut(s) 649
Bsp1286I GDGCHC 2 cut(s) 119, 379
Bsp143I GATC 4 cut(s) 109, 194, 1006, 1081
BspACI CCGC 3 cut(s) 140, 788, 973
BspANI GGCC 1 cut(s) 649
BspCNI CTCAG 1 cut(s) 898
BspHI TCATGA 1 cut(s) 445
BspLI GGNNCC 1 cut(s) 181
BspPI GGATC 1 cut(s) 1014
BsrI ACTGG 3 cut(s) 611, 739, 763
BssECI CCNNGG 1 cut(s) 514
BssMI GATC 4 cut(s) 109, 194, 1006, 1081
BssSI CACGAG 1 cut(s) 315
Bst2BI CACGAG 1 cut(s) 315
Bst2UI CCWGG 2 cut(s) 516, 1079
Bst4CI ACNGT 3 cut(s) 96, 152, 346
BstC8I GCNNGC 4 cut(s) 82, 101, 468, 923
BstDEI CTNAG 3 cut(s) 106, 672, 906
BstF5I GGATG 1 cut(s) 1057
BstKTI GATC 4 cut(s) 112, 197, 1009, 1084
BstMBI GATC 4 cut(s) 109, 194, 1006, 1081
BstNI CCWGG 2 cut(s) 516, 1079
BstNSI RCATGY 3 cut(s) 628, 925, 1073
BstSCI CCNGG 2 cut(s) 514, 1077
BstSFI CTRYAG 2 cut(s) 471, 1019
BstV1I GCAGC 3 cut(s) 574, 895, 931
BstV2I GAAGAC 1 cut(s) 11
BsuRI GGCC 1 cut(s) 649
BtsCI GGATG 1 cut(s) 1057
BtsI GCAGTG 1 cut(s) 453
BtsIMutI CAGTG 4 cut(s) 101, 119, 453, 746
Cac8I GCNNGC 4 cut(s) 82, 101, 468, 923
CciI TCATGA 1 cut(s) 445
CspCI CAANNNNNGTGG 2 cut(s) 975, 1010
CviAII CATG 7 cut(s) 66, 446, 625, 891, 922, 1066, 1070
DdeI CTNAG 3 cut(s) 106, 672, 906
DpnI GATC 4 cut(s) 111, 196, 1008, 1083
DpnII GATC 4 cut(s) 109, 194, 1006, 1081
DraI TTTAAA 1 cut(s) 403
EaeI YGGCCR 1 cut(s) 647
Eco24I GRGCYC 1 cut(s) 379
EcoRII CCWGG 2 cut(s) 514, 1077
EcoT22I ATGCAT 2 cut(s) 256, 1071
EcoT38I GRGCYC 1 cut(s) 379
FaeI CATG 7 cut(s) 69, 449, 628, 894, 925, 1069, 1073
FaqI GGGAC 1 cut(s) 296
FatI CATG 7 cut(s) 65, 445, 624, 890, 921, 1065, 1069
FauI CCCGC 2 cut(s) 133, 781
FauNDI CATATG 1 cut(s) 1045
Fnu4HI GCNGC 3 cut(s) 588, 884, 945
FokI GGATG 1 cut(s) 1064
FriOI GRGCYC 1 cut(s) 379
Fsp4HI GCNGC 3 cut(s) 588, 884, 945
FspBI CTAG 2 cut(s) 35, 948
GluI GCNGC 3 cut(s) 588, 884, 945
GsuI CTGGAG 1 cut(s) 166
HaeIII GGCC 1 cut(s) 649
HapII CCGG 2 cut(s) 591, 614
Hin1II CATG 7 cut(s) 69, 449, 628, 894, 925, 1069, 1073
HincII GTYRAC 1 cut(s) 856
HindII GTYRAC 1 cut(s) 856
HinfI GANTC 4 cut(s) 10, 309, 331, 812
HpaI GTTAAC 1 cut(s) 856
HpaII CCGG 2 cut(s) 591, 614
Hpy166II GTNNAC 1 cut(s) 856
Hpy188I TCNGA 4 cut(s) 88, 169, 724, 907
Hpy188III TCNNGA 5 cut(s) 183, 328, 335, 446, 641
Hpy8I GTNNAC 1 cut(s) 856
Hpy99I CGWCG 2 cut(s) 124, 820
HpyAV CCTTC 7 cut(s) 35, 174, 281, 334, 439, 706, 719
HpyCH4III ACNGT 3 cut(s) 96, 152, 346
HpyCH4V TGCA 5 cut(s) 99, 254, 458, 734, 1069
HpyF3I CTNAG 3 cut(s) 106, 672, 906
Hsp92II CATG 7 cut(s) 69, 449, 628, 894, 925, 1069, 1073
KspAI GTTAAC 1 cut(s) 856
Kzo9I GATC 4 cut(s) 109, 194, 1006, 1081
LmnI GCTCC 3 cut(s) 89, 185, 562
Lsp1109I GCAGC 3 cut(s) 574, 895, 931
MaeI CTAG 2 cut(s) 35, 948
MaeIII GTNAC 5 cut(s) 319, 346, 526, 667, 1054
MalI GATC 4 cut(s) 111, 196, 1008, 1083
MboI GATC 4 cut(s) 109, 194, 1006, 1081
MboII GAAGA 2 cut(s) 16, 350
MhlI GDGCHC 2 cut(s) 119, 379
MluCI AATT 6 cut(s) 212, 229, 522, 896, 901, 964
MlyI GAGTC 2 cut(s) 4, 318
MnlI CCTC 8 cut(s) 130, 324, 535, 831, 901, 983, 991, 994
Mph1103I ATGCAT 2 cut(s) 256, 1071
MroXI GAANNNNTTC 1 cut(s) 796
MseI TTAA 5 cut(s) 360, 402, 855, 879, 927
MslI CAYNNNNRTG 2 cut(s) 739, 1070
MspI CCGG 2 cut(s) 591, 614
MspR9I CCNGG 2 cut(s) 516, 1079
MvaI CCWGG 2 cut(s) 516, 1079
NdeI CATATG 1 cut(s) 1045
NdeII GATC 4 cut(s) 109, 194, 1006, 1081
NlaIII CATG 7 cut(s) 69, 449, 628, 894, 925, 1069, 1073
NlaIV GGNNCC 1 cut(s) 181
NmuCI GTSAC 3 cut(s) 346, 526, 667
NsiI ATGCAT 2 cut(s) 256, 1071
NspI RCATGY 3 cut(s) 628, 925, 1073
PaeI GCATGC 1 cut(s) 925
PagI TCATGA 1 cut(s) 445
PdmI GAANNNNTTC 1 cut(s) 796
PfeI GAWTC 2 cut(s) 331, 812
PkrI GCNGC 3 cut(s) 589, 885, 946
PleI GAGTC 2 cut(s) 4, 317
PpsI GAGTC 2 cut(s) 4, 317
Psp6I CCWGG 2 cut(s) 514, 1077
PspGI CCWGG 2 cut(s) 514, 1077
PspN4I GGNNCC 1 cut(s) 181
RseI CAYNNNNRTG 2 cut(s) 739, 1070
SaqAI TTAA 5 cut(s) 360, 402, 855, 879, 927
SatI GCNGC 3 cut(s) 588, 884, 945
Sau3AI GATC 4 cut(s) 109, 194, 1006, 1081
SchI GAGTC 2 cut(s) 4, 318
ScrFI CCNGG 2 cut(s) 516, 1079
SduI GDGCHC 2 cut(s) 119, 379
SfcI CTRYAG 2 cut(s) 471, 1019
SmiMI CAYNNNNRTG 2 cut(s) 739, 1070
SphI GCATGC 1 cut(s) 925
Sse9I AATT 6 cut(s) 212, 229, 522, 896, 901, 964
SsiI CCGC 3 cut(s) 140, 788, 973
SspMI CTAG 2 cut(s) 35, 948
StyD4I CCNGG 2 cut(s) 514, 1077
TaaI ACNGT 3 cut(s) 96, 152, 346
TaqI TCGA 2 cut(s) 119, 285
TasI AATT 6 cut(s) 212, 229, 522, 896, 901, 964
TfiI GAWTC 2 cut(s) 331, 812
Tru1I TTAA 5 cut(s) 360, 402, 855, 879, 927
Tru9I TTAA 5 cut(s) 360, 402, 855, 879, 927
TscAI CASTG 4 cut(s) 101, 119, 460, 746
TseFI GTSAC 3 cut(s) 346, 526, 667
TseI GCWGC 3 cut(s) 587, 883, 944
Tsp45I GTSAC 3 cut(s) 346, 526, 667
TspDTI ATGAA 5 cut(s) 17, 54, 501, 614, 797
TspGWI ACGGA 3 cut(s) 137, 141, 579
TspRI CASTG 4 cut(s) 101, 119, 460, 746
XapI RAATTY 1 cut(s) 964
XceI RCATGY 3 cut(s) 628, 925, 1073
XmnI GAANNNNTTC 1 cut(s) 796
XspI CTAG 2 cut(s) 35, 948
Zsp2I ATGCAT 2 cut(s) 256, 1071
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.