RchiOBHm_Chr1g0320591

PI-PLC X domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001750
Physical Location & Seq
Reverse (-)
8245080 .. 8247373
2294 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55073

Sequence Viewer

Length: 993 bp
ATGGACAATCAGTGCTCGACGGACGGAGATTGTGAGGCGGGGCTTTACTGTTTCGGTTGCCCCTCGGAGTTTTCTGGCTCCAGATGTGTGAGATCAACCACCACTAACCAATTCCAGCTTTTGAATAATTCTCTACCATTCAACAAATATGCATTTTTGACAACCCACAATGCTTTTGCTATCGAAGGAGAGCCATCTCATACTGGAGTCCCCCGTATTACCTTCACGAATCAAGAAGATACTGTCACTCAACAACTTAACAATGGAGTTAGAGCTCTAATGCTTGATACCTATGATTTTAAAGGAGATGCCTGGTTGTGCCATTCCTTCAAAGGAAAATGCCATGACTACACTGCATTTGAGCCAGCTATAGATACATTGAATGAAATCCAAGCATTTTTATCAGCAAACCCAGGAGAAATTGTGACATTGATATTAGAGGACTATGTTGAAGCTCCAAACGGATTGACAAATGTTTTCAAAGCTGCCGGATTGATGAAATACTGGTTTCCGGTATCAAACATGCCCAAAAGTGGTCAGGATTGGCCGCTGGTTAGCGATATGGTCACTAAGAACCAAAGGCTACTTGTATTCACTTCAAAACAAGAGAAGGAACAATCCGAAGGGATTGCATACCAGTGGAACTACATGGTTGAAAACCAGTATGGAAATGATGGAATGAAAGCGGGAAGCTGTTCAAACAGAGCTGAATCGTCTCCTCTAAATGACAAGACTAAATCATTGGTGTTGGTTAACTATTTTGGGTCAGTTCCCATTAAGCAACTCTCATGTCAATTCAATTCTGAGGATTTGGTTAGCATGCTTAACACTTGCTATGGTGCTGCTGGAAACCGATGGGCGAATTTTGTTGCGGTTGATTTTTACAAGAGGAGTGGAGGAGGGGGATCATTTCAAGCTACAGACACTCTCAATGGAGAACTCATATGTGGATGTAACGATGTCCATGCATGTGTGGTAAGCTATGAAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

330

Amino Acids

36.5

Weight (kDa)

4.8

Isoelectric Point (pI)

35.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC_cat PF26178 41 - 324 3.2e-149 PI-PLC-like catalytic domain
PI-PLC_X PF26146 72 - 296 8.4e-24 PI-PLC X
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 38, 548, 686, 872
AclWI GGATC 1 cut(s) 913
AcoI YGGCCR 1 cut(s) 545
AcsI RAATTY 1 cut(s) 862
AfiI CCNNNNNNNGG 1 cut(s) 533
AjnI CCWGG 2 cut(s) 311, 412
AluBI AGCT 9 cut(s) 118, 275, 368, 455, 485, 693, 707, 917, 981
AluI AGCT 9 cut(s) 118, 275, 368, 455, 485, 693, 707, 917, 981
Alw21I GWGCWC 2 cut(s) 17, 277
Alw26I GTCTC 1 cut(s) 720
AlwI GGATC 1 cut(s) 913
AoxI GGCC 1 cut(s) 545
ApeKI GCWGC 2 cut(s) 485, 842
ApoI RAATTY 1 cut(s) 862
Asp700I GAANNNNTTC 1 cut(s) 694
BanII GRGCYC 1 cut(s) 277
Bbv12I GWGCWC 2 cut(s) 17, 277
BbvI GCAGC 2 cut(s) 472, 829
BccI CCATC 3 cut(s) 202, 668, 849
BcgI CGANNNNNNTGC 4 cut(s) 611, 645, 947, 981
BciT130I CCWGG 2 cut(s) 313, 414
BcoDI GTCTC 1 cut(s) 720
BfmI CTRYAG 2 cut(s) 369, 918
BisI GCNGC 3 cut(s) 486, 548, 843
BlsI GCNGC 3 cut(s) 487, 549, 844
Bme1390I CCNGG 2 cut(s) 313, 414
BmiI GGNNCC 1 cut(s) 79
BmrFI CCNGG 2 cut(s) 313, 414
BmsI GCATC 1 cut(s) 298
BpmI CTGGAG 2 cut(s) 64, 225
BsaJI CCNNGG 2 cut(s) 63, 412
BsaWI WCCGGW 1 cut(s) 511
BsaXI ACNNNNNCTCC 2 cut(s) 408, 438
Bsc4I CCNNNNNNNGG 1 cut(s) 533
Bse1I ACTGG 4 cut(s) 208, 509, 637, 661
BseBI CCWGG 2 cut(s) 313, 414
BseDI CCNNGG 2 cut(s) 63, 412
BseGI GGATG 1 cut(s) 956
BseLI CCNNNNNNNGG 1 cut(s) 533
BseMII CTCAG 1 cut(s) 795
BseNI ACTGG 4 cut(s) 208, 509, 637, 661
BseRI GAGGAG 3 cut(s) 708, 904, 912
BseXI GCAGC 2 cut(s) 472, 829
BshFI GGCC 1 cut(s) 547
BsiHKAI GWGCWC 2 cut(s) 17, 277
BsiSI CCGG 2 cut(s) 489, 512
BslFI GGGAC 1 cut(s) 194
BslI CCNNNNNNNGG 1 cut(s) 533
BsmAI GTCTC 1 cut(s) 720
BsmBI CGTCTC 1 cut(s) 720
BsmFI GGGAC 1 cut(s) 194
BsnI GGCC 1 cut(s) 547
Bsp1286I GDGCHC 2 cut(s) 17, 277
Bsp143I GATC 2 cut(s) 92, 905
BspACI CCGC 4 cut(s) 38, 548, 686, 872
BspANI GGCC 1 cut(s) 547
BspCNI CTCAG 1 cut(s) 796
BspLI GGNNCC 1 cut(s) 79
BspPI GGATC 1 cut(s) 913
BsrI ACTGG 4 cut(s) 208, 509, 637, 661
BssECI CCNNGG 2 cut(s) 63, 412
BssMI GATC 2 cut(s) 92, 905
Bst2UI CCWGG 2 cut(s) 313, 414
Bst4CI ACNGT 2 cut(s) 50, 244
BstC8I GCNNGC 2 cut(s) 366, 821
BstDEI CTNAG 2 cut(s) 570, 804
BstF5I GGATG 1 cut(s) 956
BstKTI GATC 2 cut(s) 95, 908
BstMAI GTCTC 1 cut(s) 720
BstMBI GATC 2 cut(s) 92, 905
BstNI CCWGG 2 cut(s) 313, 414
BstNSI RCATGY 3 cut(s) 526, 823, 972
BstSCI CCNGG 2 cut(s) 311, 412
BstSFI CTRYAG 2 cut(s) 369, 918
BstV1I GCAGC 2 cut(s) 472, 829
BsuRI GGCC 1 cut(s) 547
BtsCI GGATG 1 cut(s) 956
BtsI GCAGTG 1 cut(s) 351
BtsIMutI CAGTG 3 cut(s) 17, 351, 644
Cac8I GCNNGC 2 cut(s) 366, 821
CspCI CAANNNNNGTGG 2 cut(s) 874, 909
CviAII CATG 7 cut(s) 344, 523, 649, 789, 820, 965, 969
DdeI CTNAG 2 cut(s) 570, 804
DpnI GATC 2 cut(s) 94, 907
DpnII GATC 2 cut(s) 92, 905
DraI TTTAAA 1 cut(s) 301
EaeI YGGCCR 1 cut(s) 545
Ecl136II GAGCTC 1 cut(s) 275
Eco24I GRGCYC 1 cut(s) 277
Eco53kI GAGCTC 1 cut(s) 275
EcoICRI GAGCTC 1 cut(s) 275
EcoRII CCWGG 2 cut(s) 311, 412
EcoT22I ATGCAT 2 cut(s) 154, 970
EcoT38I GRGCYC 1 cut(s) 277
Esp3I CGTCTC 1 cut(s) 720
FaeI CATG 7 cut(s) 347, 526, 652, 792, 823, 968, 972
FaqI GGGAC 1 cut(s) 194
FatI CATG 7 cut(s) 343, 522, 648, 788, 819, 964, 968
FauI CCCGC 2 cut(s) 31, 679
FauNDI CATATG 1 cut(s) 944
Fnu4HI GCNGC 3 cut(s) 486, 548, 843
FokI GGATG 1 cut(s) 963
FriOI GRGCYC 1 cut(s) 277
Fsp4HI GCNGC 3 cut(s) 486, 548, 843
GluI GCNGC 3 cut(s) 486, 548, 843
GsuI CTGGAG 2 cut(s) 64, 225
HaeIII GGCC 1 cut(s) 547
HapII CCGG 2 cut(s) 489, 512
Hin1II CATG 7 cut(s) 347, 526, 652, 792, 823, 968, 972
HincII GTYRAC 1 cut(s) 754
HindII GTYRAC 1 cut(s) 754
HinfI GANTC 3 cut(s) 207, 229, 710
HpaI GTTAAC 1 cut(s) 754
HpaII CCGG 2 cut(s) 489, 512
Hpy166II GTNNAC 1 cut(s) 754
Hpy188I TCNGA 3 cut(s) 67, 622, 805
Hpy188III TCNNGA 4 cut(s) 81, 226, 233, 539
Hpy8I GTNNAC 1 cut(s) 754
Hpy99I CGWCG 1 cut(s) 22
HpyAV CCTTC 5 cut(s) 179, 232, 337, 604, 617
HpyCH4III ACNGT 2 cut(s) 50, 244
HpyCH4V TGCA 4 cut(s) 152, 356, 632, 968
HpyF3I CTNAG 2 cut(s) 570, 804
Hsp92II CATG 7 cut(s) 347, 526, 652, 792, 823, 968, 972
KspAI GTTAAC 1 cut(s) 754
Kzo9I GATC 2 cut(s) 92, 905
LmnI GCTCC 2 cut(s) 83, 460
Lsp1109I GCAGC 2 cut(s) 472, 829
LweI GCATC 1 cut(s) 298
MaeIII GTNAC 4 cut(s) 244, 424, 565, 953
MalI GATC 2 cut(s) 94, 907
MboI GATC 2 cut(s) 92, 905
MboII GAAGA 1 cut(s) 248
MhlI GDGCHC 2 cut(s) 17, 277
MluCI AATT 6 cut(s) 110, 127, 420, 794, 799, 862
MlyI GAGTC 1 cut(s) 216
MnlI CCTC 8 cut(s) 28, 73, 433, 729, 799, 882, 890, 893
Mph1103I ATGCAT 2 cut(s) 154, 970
MroXI GAANNNNTTC 1 cut(s) 694
MseI TTAA 5 cut(s) 258, 300, 753, 777, 825
MslI CAYNNNNRTG 2 cut(s) 637, 969
MspA1I CMGCKG 1 cut(s) 550
MspI CCGG 2 cut(s) 489, 512
MspR9I CCNGG 2 cut(s) 313, 414
MvaI CCWGG 2 cut(s) 313, 414
NdeI CATATG 1 cut(s) 944
NdeII GATC 2 cut(s) 92, 905
NlaIII CATG 7 cut(s) 347, 526, 652, 792, 823, 968, 972
NlaIV GGNNCC 1 cut(s) 79
NmuCI GTSAC 3 cut(s) 244, 424, 565
NsiI ATGCAT 2 cut(s) 154, 970
NspI RCATGY 3 cut(s) 526, 823, 972
PaeI GCATGC 1 cut(s) 823
PdmI GAANNNNTTC 1 cut(s) 694
PfeI GAWTC 2 cut(s) 229, 710
PkrI GCNGC 3 cut(s) 487, 549, 844
PleI GAGTC 1 cut(s) 215
PpsI GAGTC 1 cut(s) 215
Psp124BI GAGCTC 1 cut(s) 277
Psp6I CCWGG 2 cut(s) 311, 412
PspGI CCWGG 2 cut(s) 311, 412
PspN4I GGNNCC 1 cut(s) 79
RseI CAYNNNNRTG 2 cut(s) 637, 969
SacI GAGCTC 1 cut(s) 277
SaqAI TTAA 5 cut(s) 258, 300, 753, 777, 825
SatI GCNGC 3 cut(s) 486, 548, 843
Sau3AI GATC 2 cut(s) 92, 905
SchI GAGTC 1 cut(s) 216
ScrFI CCNGG 2 cut(s) 313, 414
SduI GDGCHC 2 cut(s) 17, 277
SfaNI GCATC 1 cut(s) 298
SfcI CTRYAG 2 cut(s) 369, 918
SmiMI CAYNNNNRTG 2 cut(s) 637, 969
SphI GCATGC 1 cut(s) 823
Sse9I AATT 6 cut(s) 110, 127, 420, 794, 799, 862
SsiI CCGC 4 cut(s) 38, 548, 686, 872
SstI GAGCTC 1 cut(s) 277
StyD4I CCNGG 2 cut(s) 311, 412
TaaI ACNGT 2 cut(s) 50, 244
TaqI TCGA 2 cut(s) 17, 183
TasI AATT 6 cut(s) 110, 127, 420, 794, 799, 862
TauI GCSGC 1 cut(s) 550
TfiI GAWTC 2 cut(s) 229, 710
Tru1I TTAA 5 cut(s) 258, 300, 753, 777, 825
Tru9I TTAA 5 cut(s) 258, 300, 753, 777, 825
TscAI CASTG 3 cut(s) 17, 358, 644
TseFI GTSAC 3 cut(s) 244, 424, 565
TseI GCWGC 2 cut(s) 485, 842
Tsp45I GTSAC 3 cut(s) 244, 424, 565
TspDTI ATGAA 3 cut(s) 399, 512, 695
TspGWI ACGGA 3 cut(s) 35, 39, 477
TspRI CASTG 3 cut(s) 17, 358, 644
XapI RAATTY 1 cut(s) 862
XceI RCATGY 3 cut(s) 526, 823, 972
XmnI GAANNNNTTC 1 cut(s) 694
Zsp2I ATGCAT 2 cut(s) 154, 970
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.