Rroxscaffold_2G00086050

PI-PLC X domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001750
Physical Location & Seq
Reverse (-)
8348049 .. 8352662
4614 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00086050.1

Sequence Viewer

Length: 1080 bp
ATGAAGACGACTCGGCACAGCTTCCTTCTGGTTCTACTTCTGGTTGTGTCTCTTGCTGTGTTTTCATGTGTCGTCATAGCTTGCTCCGACGGACAGTGCAAGCTCTTAGATCAGTGCTCGACGGACGGAGATTGTGAGGCGGGGCTTTACTGTTTCGGTTGCCCTTTGGAGTTTTTAGGCTCCAGATGTGTGAGATCGACCACCACCAACCAATTCCAGCTTTTGAATAGTTCTCTACCATTCAACAAATATGCATTTTTGACAACCCACAATGCTTTTGCCATCGAAGGAGAGCCATCTCATACCGGAGTCCCTCGTGTTACCTTGACGAATCAAGAAGACACTGTCACTAAACAACTTAACAATGGAGTTAGAGCCACGATGCTTGATACCTATGATTTTAAAGGAGATGTCCGGTTGTGCCATTCCTTCAAAGGACAATGCCATGACTACACTGCATTTGAGCCAGCTATAGATACATTAAACGAAATCCAAGCATTTTTATCAGCAAATCCAGGAGAAATCGTGACATTGATATTAGAGGACTATGTTGAAGCTTCAAATGGACTGACAAATGTTTTCAAATCTGCCGGATTGATGAAATACTGGTTTCCGATATCAAACATGCCCAAAAGTGGTCGGGATTGGCCGCTGGTTAGCGATATGGTTGCCAAGAACCAAAGGCTACTTGTATTCACTTCAAAACAAGAGAAGGAACAATCCGAAGGGATTGCATACCAGTGGAACTACATGGTTGAAAACGAGTATGGAGATGATGGAATGGAAGCGGGAAGCTGTTCAAACAGAGGTGAATCGTCGCCTCTAAATGACAAGACTAAATCATTGGTGTTGGTTAACCATTTTGGGTCATTTCCCATTAAGCAGCTCTCATGTCAATTCAATTCGAGGATTTGGTTAGCATGCTTTACACTTGCTATTGTGCTGCTGGAAACCGGTGGGCGAATTTTGTTGCGGTTGATTTTTACAAGAGGAGTGGAGGGGGAGGATCGTTTCAAGCTACGGACACTCTCAATGGAGAACTCATATGTGGATGTAACGATGTCCATGCATGTGTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

359

Amino Acids

40.18

Weight (kDa)

5.45

Isoelectric Point (pI)

33.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC_cat PF26178 76 - 304 2.1e-114 PI-PLC-like catalytic domain
PI-PLC_X PF26146 106 - 295 2.3e-23 PI-PLC X
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 140, 650, 788, 973
AclWI GGATC 1 cut(s) 1014
AcoI YGGCCR 1 cut(s) 647
AcsI RAATTY 1 cut(s) 963
AfiI CCNNNNNNNGG 1 cut(s) 635
AgeI ACCGGT 1 cut(s) 953
AjnI CCWGG 1 cut(s) 514
AluBI AGCT 9 cut(s) 21, 80, 103, 220, 470, 557, 795, 886, 1018
AluI AGCT 9 cut(s) 21, 80, 103, 220, 470, 557, 795, 886, 1018
Alw21I GWGCWC 1 cut(s) 119
Alw26I GTCTC 1 cut(s) 54
AlwI GGATC 1 cut(s) 1014
AoxI GGCC 1 cut(s) 647
ApeKI GCWGC 2 cut(s) 883, 943
ApoI RAATTY 1 cut(s) 963
AsiGI ACCGGT 1 cut(s) 953
Asp700I GAANNNNTTC 1 cut(s) 796
AsuHPI GGTGA 1 cut(s) 821
BauI CACGAG 1 cut(s) 315
BbsI GAAGAC 2 cut(s) 11, 345
Bbv12I GWGCWC 1 cut(s) 119
BbvI GCAGC 2 cut(s) 895, 930
BccI CCATC 3 cut(s) 290, 304, 770
BcgI CGANNNNNNTGC 5 cut(s) 650, 684, 713, 747, 1048
BciT130I CCWGG 1 cut(s) 516
BcoDI GTCTC 1 cut(s) 54
BfmI CTRYAG 1 cut(s) 471
BisI GCNGC 3 cut(s) 650, 884, 944
BlsI GCNGC 3 cut(s) 651, 885, 945
Bme1390I CCNGG 1 cut(s) 516
BmiI GGNNCC 1 cut(s) 181
BmrFI CCNGG 1 cut(s) 516
BmsI GCATC 1 cut(s) 372
BpiI GAAGAC 2 cut(s) 11, 345
BpmI CTGGAG 1 cut(s) 166
BsaWI WCCGGW 3 cut(s) 305, 414, 953
BsaXI ACNNNNNCTCC 2 cut(s) 510, 540
Bsc4I CCNNNNNNNGG 1 cut(s) 635
Bse118I RCCGGY 1 cut(s) 953
Bse1I ACTGG 2 cut(s) 611, 739
BseBI CCWGG 1 cut(s) 516
BseGI GGATG 1 cut(s) 1057
BseLI CCNNNNNNNGG 1 cut(s) 635
BseNI ACTGG 2 cut(s) 611, 739
BseRI GAGGAG 1 cut(s) 1005
BseXI GCAGC 2 cut(s) 895, 930
BshFI GGCC 1 cut(s) 649
BshTI ACCGGT 1 cut(s) 953
BsiHKAI GWGCWC 1 cut(s) 119
BsiSI CCGG 4 cut(s) 306, 415, 591, 954
BslFI GGGAC 1 cut(s) 296
BslI CCNNNNNNNGG 1 cut(s) 635
BsmAI GTCTC 1 cut(s) 54
BsmFI GGGAC 1 cut(s) 296
BsnI GGCC 1 cut(s) 649
Bsp1286I GDGCHC 1 cut(s) 119
Bsp143I GATC 3 cut(s) 109, 194, 1006
BspACI CCGC 4 cut(s) 140, 650, 788, 973
BspANI GGCC 1 cut(s) 649
BspLI GGNNCC 1 cut(s) 181
BspPI GGATC 1 cut(s) 1014
BsrFI RCCGGY 1 cut(s) 953
BsrI ACTGG 2 cut(s) 611, 739
BssAI RCCGGY 1 cut(s) 953
BssMI GATC 3 cut(s) 109, 194, 1006
BssSI CACGAG 1 cut(s) 315
Bst2BI CACGAG 1 cut(s) 315
Bst2UI CCWGG 1 cut(s) 516
Bst4CI ACNGT 3 cut(s) 96, 152, 346
BstC8I GCNNGC 4 cut(s) 82, 101, 468, 922
BstDEI CTNAG 1 cut(s) 106
BstF5I GGATG 1 cut(s) 1057
BstKTI GATC 3 cut(s) 112, 197, 1009
BstMAI GTCTC 1 cut(s) 54
BstMBI GATC 3 cut(s) 109, 194, 1006
BstNI CCWGG 1 cut(s) 516
BstNSI RCATGY 3 cut(s) 628, 924, 1073
BstSCI CCNGG 1 cut(s) 514
BstSFI CTRYAG 1 cut(s) 471
BstV1I GCAGC 2 cut(s) 895, 930
BstV2I GAAGAC 2 cut(s) 11, 345
BsuRI GGCC 1 cut(s) 649
BtsCI GGATG 1 cut(s) 1057
BtsI GCAGTG 1 cut(s) 453
BtsIMutI CAGTG 5 cut(s) 101, 119, 342, 453, 746
Cac8I GCNNGC 4 cut(s) 82, 101, 468, 922
Cfr10I RCCGGY 1 cut(s) 953
CspAI ACCGGT 1 cut(s) 953
CspCI CAANNNNNGTGG 4 cut(s) 367, 402, 975, 1010
CviAII CATG 8 cut(s) 66, 446, 625, 751, 891, 921, 1066, 1070
DdeI CTNAG 1 cut(s) 106
DpnI GATC 3 cut(s) 111, 196, 1008
DpnII GATC 3 cut(s) 109, 194, 1006
DraI TTTAAA 1 cut(s) 403
EaeI YGGCCR 1 cut(s) 647
Eco32I GATATC 1 cut(s) 618
EcoRII CCWGG 1 cut(s) 514
EcoRV GATATC 1 cut(s) 618
EcoT22I ATGCAT 2 cut(s) 256, 1071
FaeI CATG 8 cut(s) 69, 449, 628, 754, 894, 924, 1069, 1073
FaqI GGGAC 1 cut(s) 296
FatI CATG 8 cut(s) 65, 445, 624, 750, 890, 920, 1065, 1069
FauI CCCGC 2 cut(s) 133, 781
FauNDI CATATG 1 cut(s) 1045
Fnu4HI GCNGC 3 cut(s) 650, 884, 944
FokI GGATG 1 cut(s) 1064
Fsp4HI GCNGC 3 cut(s) 650, 884, 944
GluI GCNGC 3 cut(s) 650, 884, 944
GsuI CTGGAG 1 cut(s) 166
HaeIII GGCC 1 cut(s) 649
HapII CCGG 4 cut(s) 306, 415, 591, 954
Hin1II CATG 8 cut(s) 69, 449, 628, 754, 894, 924, 1069, 1073
HincII GTYRAC 1 cut(s) 856
HindII GTYRAC 1 cut(s) 856
HindIII AAGCTT 1 cut(s) 555
HinfI GANTC 4 cut(s) 10, 309, 331, 812
HpaI GTTAAC 1 cut(s) 856
HpaII CCGG 4 cut(s) 306, 415, 591, 954
HphI GGTGA 1 cut(s) 821
Hpy166II GTNNAC 1 cut(s) 856
Hpy188I TCNGA 3 cut(s) 88, 615, 724
Hpy188III TCNNGA 4 cut(s) 183, 335, 526, 641
Hpy8I GTNNAC 1 cut(s) 856
Hpy99I CGWCG 3 cut(s) 92, 124, 820
HpyAV CCTTC 5 cut(s) 35, 281, 439, 706, 719
HpyCH4III ACNGT 3 cut(s) 96, 152, 346
HpyCH4V TGCA 5 cut(s) 99, 254, 458, 734, 1069
HpyF3I CTNAG 1 cut(s) 106
Hsp92II CATG 8 cut(s) 69, 449, 628, 754, 894, 924, 1069, 1073
KspAI GTTAAC 1 cut(s) 856
Kzo9I GATC 3 cut(s) 109, 194, 1006
LmnI GCTCC 2 cut(s) 89, 185
Lsp1109I GCAGC 2 cut(s) 895, 930
LweI GCATC 1 cut(s) 372
MaeIII GTNAC 4 cut(s) 319, 346, 526, 1054
MalI GATC 3 cut(s) 111, 196, 1008
MboI GATC 3 cut(s) 109, 194, 1006
MboII GAAGA 2 cut(s) 16, 350
MhlI GDGCHC 1 cut(s) 119
MluCI AATT 4 cut(s) 212, 896, 901, 963
MlyI GAGTC 2 cut(s) 4, 318
MmeI TCCRAC 1 cut(s) 111
MnlI CCTC 9 cut(s) 130, 324, 535, 800, 831, 900, 983, 991, 997
Mph1103I ATGCAT 2 cut(s) 256, 1071
MroXI GAANNNNTTC 1 cut(s) 796
MseI TTAA 5 cut(s) 360, 402, 482, 855, 879
MslI CAYNNNNRTG 2 cut(s) 739, 1070
MspA1I CMGCKG 1 cut(s) 652
MspI CCGG 4 cut(s) 306, 415, 591, 954
MspR9I CCNGG 1 cut(s) 516
MvaI CCWGG 1 cut(s) 516
NdeI CATATG 1 cut(s) 1045
NdeII GATC 3 cut(s) 109, 194, 1006
NlaIII CATG 8 cut(s) 69, 449, 628, 754, 894, 924, 1069, 1073
NlaIV GGNNCC 1 cut(s) 181
NmuCI GTSAC 2 cut(s) 346, 526
NsiI ATGCAT 2 cut(s) 256, 1071
NspI RCATGY 3 cut(s) 628, 924, 1073
PaeI GCATGC 1 cut(s) 924
PdmI GAANNNNTTC 1 cut(s) 796
PfeI GAWTC 2 cut(s) 331, 812
PflFI GACNNNGTC 1 cut(s) 344
PfoI TCCNGGA 1 cut(s) 514
PinAI ACCGGT 1 cut(s) 953
PkrI GCNGC 3 cut(s) 651, 885, 945
PleI GAGTC 2 cut(s) 4, 317
PpsI GAGTC 2 cut(s) 4, 317
Psp6I CCWGG 1 cut(s) 514
PspGI CCWGG 1 cut(s) 514
PspN4I GGNNCC 1 cut(s) 181
PsyI GACNNNGTC 1 cut(s) 344
RseI CAYNNNNRTG 2 cut(s) 739, 1070
SaqAI TTAA 5 cut(s) 360, 402, 482, 855, 879
SatI GCNGC 3 cut(s) 650, 884, 944
Sau3AI GATC 3 cut(s) 109, 194, 1006
SchI GAGTC 2 cut(s) 4, 318
ScrFI CCNGG 1 cut(s) 516
SduI GDGCHC 1 cut(s) 119
SfaNI GCATC 1 cut(s) 372
SfcI CTRYAG 1 cut(s) 471
SmiMI CAYNNNNRTG 2 cut(s) 739, 1070
SphI GCATGC 1 cut(s) 924
Sse9I AATT 4 cut(s) 212, 896, 901, 963
SsiI CCGC 4 cut(s) 140, 650, 788, 973
StyD4I CCNGG 1 cut(s) 514
TaaI ACNGT 3 cut(s) 96, 152, 346
TaqI TCGA 4 cut(s) 119, 197, 285, 905
TasI AATT 4 cut(s) 212, 896, 901, 963
TauI GCSGC 1 cut(s) 652
TfiI GAWTC 2 cut(s) 331, 812
Tru1I TTAA 5 cut(s) 360, 402, 482, 855, 879
Tru9I TTAA 5 cut(s) 360, 402, 482, 855, 879
TscAI CASTG 5 cut(s) 101, 119, 349, 460, 746
TseFI GTSAC 2 cut(s) 346, 526
TseI GCWGC 2 cut(s) 883, 943
Tsp45I GTSAC 2 cut(s) 346, 526
TspDTI ATGAA 3 cut(s) 17, 54, 614
TspGWI ACGGA 4 cut(s) 105, 137, 141, 1036
TspRI CASTG 5 cut(s) 101, 119, 349, 460, 746
Tth111I GACNNNGTC 1 cut(s) 344
XapI RAATTY 1 cut(s) 963
XceI RCATGY 3 cut(s) 628, 924, 1073
XmnI GAANNNNTTC 1 cut(s) 796
Zsp2I ATGCAT 2 cut(s) 256, 1071
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.