Rw2G048210

PI-PLC X domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001750
Physical Location & Seq
Forward (+)
75650152 .. 75654678
4527 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G048210.1

Sequence Viewer

Length: 1119 bp
ATGAAGATGACTCGGCACAGCTTCCTTCTGGTTCTACTTCTGGTTATGTCTCTTGCTGTGTTTTCATGTGTCGTCATAGCTTGCTCCGACGGACAGTGCAAGCTCTTAGATCAGTGCTCGACAGACGGAGATTGTGAGGCGGGGCTTTACTGTTTCAGTTGCCCTTTTGAGTTTTTAGGCTCCAGATGTGTGAGATCAACCACCACCAACCAATTCCAGCTTTTGAATAGTTCTCTACCATTCAACAAATATGCATTTTTGACAACCCACAATGCTTTTGCCATCGAAGGAGAGCCATCTCATACCGGAGTCCCTCGTGTTACCTTGACGAATCAAGAAGACACTGTCACTCAACAACTTAACAATGGAGTTAGAGCCCTGATGCTTGATACCTATGATTTTAAAGGAGATGTCTGGTTGTGCCATTCCTTCAAAGGACAATGCCATGACTACACTGCATTTGAGCCAGCTGTAGATACATTAAACGAAATCCAAGCATTTTTATCAGCAAATCCAGGAGAAATCGTGACATTGATATTAGAGGACTATGTTGAAGCTTTAAATGGACTGACAAATGTTTTCAAATCTGCCGGATTGATGAAATACTGGTTTCCGGTATCAAACATGCCCAAAAGTGGTCAGGATTGGCCGCTGGTTAGCGATATGGTTGCCAAGAACCAAAGGCTACTTGTATTCACTTCAAAACAAGAGAAGGAACAATCCGAAGGGATTGCATACCAGTGGAACTACATGGTTGAAAACGAGTATGGAGATGATGGAATGGAAGCTGGAAGCTGTTCAAACAGAGGTGAATCGTCGCCTCTAAATGACAAGACTAAATCATTGGTGTTGGTTAACTATTTTGGGTCATTTCCCATTAAGCAGCTCTCATGTCAATTCAATTCTGAGGATTTGGTTAGCATGCTTTACACTTGCTATTGTGCTGCTAGAAACCGGTGGGCGAATTTTGTTGCGGTTGATTTTTACAAAAGGAGTGGAGGGGGAGGATCATTTCAAGCTACAGACACTCTCAATGGAGAACTCATATGTGGATGTAACGATGTCCATGCATGTGTGGGAAGTTGCTTTATTGGATTTTTTAAAGAGTTAAATCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

372

Amino Acids

41.36

Weight (kDa)

4.85

Isoelectric Point (pI)

37.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC_cat PF26178 76 - 358 4.1e-146 PI-PLC-like catalytic domain
PI-PLC_X PF26146 106 - 296 2e-23 PI-PLC X
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 140, 650, 974
AclWI GGATC 1 cut(s) 1015
AcoI YGGCCR 1 cut(s) 647
AcsI RAATTY 1 cut(s) 964
AfiI CCNNNNNNNGG 1 cut(s) 635
AgeI ACCGGT 1 cut(s) 954
AjnI CCWGG 1 cut(s) 514
Alw21I GWGCWC 1 cut(s) 119
Alw26I GTCTC 1 cut(s) 54
AlwI GGATC 1 cut(s) 1015
AoxI GGCC 1 cut(s) 647
ApeKI GCWGC 2 cut(s) 883, 944
ApoI RAATTY 1 cut(s) 964
AsiGI ACCGGT 1 cut(s) 954
Asp700I GAANNNNTTC 1 cut(s) 796
AsuHPI GGTGA 1 cut(s) 821
BanII GRGCYC 1 cut(s) 379
BauI CACGAG 1 cut(s) 315
BbsI GAAGAC 1 cut(s) 345
Bbv12I GWGCWC 1 cut(s) 119
BbvI GCAGC 2 cut(s) 895, 931
BccI CCATC 3 cut(s) 290, 304, 770
BcgI CGANNNNNNTGC 6 cut(s) 650, 684, 713, 747, 1049, 1083
BciT130I CCWGG 1 cut(s) 516
BcoDI GTCTC 1 cut(s) 54
BfaI CTAG 1 cut(s) 948
BfmI CTRYAG 3 cut(s) 471, 1020, 1115
BisI GCNGC 3 cut(s) 650, 884, 945
BlsI GCNGC 3 cut(s) 651, 885, 946
Bme1390I CCNGG 1 cut(s) 516
BmiI GGNNCC 1 cut(s) 181
BmrFI CCNGG 1 cut(s) 516
BmsI GCATC 1 cut(s) 372
BpiI GAAGAC 1 cut(s) 345
BpmI CTGGAG 1 cut(s) 166
BsaWI WCCGGW 3 cut(s) 305, 613, 954
BsaXI ACNNNNNCTCC 2 cut(s) 510, 540
Bsc4I CCNNNNNNNGG 1 cut(s) 635
Bse118I RCCGGY 1 cut(s) 954
Bse1I ACTGG 2 cut(s) 611, 739
BseBI CCWGG 1 cut(s) 516
BseGI GGATG 1 cut(s) 1058
BseLI CCNNNNNNNGG 1 cut(s) 635
BseMII CTCAG 1 cut(s) 897
BseNI ACTGG 2 cut(s) 611, 739
BseXI GCAGC 2 cut(s) 895, 931
BshFI GGCC 1 cut(s) 649
BshTI ACCGGT 1 cut(s) 954
BsiHKAI GWGCWC 1 cut(s) 119
BsiSI CCGG 4 cut(s) 306, 591, 614, 955
BslFI GGGAC 1 cut(s) 296
BslI CCNNNNNNNGG 1 cut(s) 635
BsmAI GTCTC 1 cut(s) 54
BsmFI GGGAC 1 cut(s) 296
BsnI GGCC 1 cut(s) 649
Bsp1286I GDGCHC 2 cut(s) 119, 379
Bsp143I GATC 3 cut(s) 109, 194, 1007
BspACI CCGC 3 cut(s) 140, 650, 974
BspANI GGCC 1 cut(s) 649
BspCNI CTCAG 1 cut(s) 898
BspLI GGNNCC 1 cut(s) 181
BspPI GGATC 1 cut(s) 1015
BsrFI RCCGGY 1 cut(s) 954
BsrI ACTGG 2 cut(s) 611, 739
BssAI RCCGGY 1 cut(s) 954
BssMI GATC 3 cut(s) 109, 194, 1007
BssSI CACGAG 1 cut(s) 315
Bst2BI CACGAG 1 cut(s) 315
Bst2UI CCWGG 1 cut(s) 516
Bst4CI ACNGT 3 cut(s) 96, 152, 346
BstC8I GCNNGC 4 cut(s) 82, 101, 468, 923
BstDEI CTNAG 2 cut(s) 106, 906
BstF5I GGATG 1 cut(s) 1058
BstKTI GATC 3 cut(s) 112, 197, 1010
BstMAI GTCTC 1 cut(s) 54
BstMBI GATC 3 cut(s) 109, 194, 1007
BstNI CCWGG 1 cut(s) 516
BstNSI RCATGY 3 cut(s) 628, 925, 1074
BstSCI CCNGG 1 cut(s) 514
BstSFI CTRYAG 3 cut(s) 471, 1020, 1115
BstV1I GCAGC 2 cut(s) 895, 931
BstV2I GAAGAC 1 cut(s) 345
BsuRI GGCC 1 cut(s) 649
BtsCI GGATG 1 cut(s) 1058
BtsI GCAGTG 1 cut(s) 453
BtsIMutI CAGTG 5 cut(s) 101, 119, 342, 453, 746
Cac8I GCNNGC 4 cut(s) 82, 101, 468, 923
Cfr10I RCCGGY 1 cut(s) 954
CspAI ACCGGT 1 cut(s) 954
CspCI CAANNNNNGTGG 2 cut(s) 976, 1011
CviAII CATG 8 cut(s) 66, 446, 625, 751, 891, 922, 1067, 1071
DdeI CTNAG 2 cut(s) 106, 906
DpnI GATC 3 cut(s) 111, 196, 1009
DpnII GATC 3 cut(s) 109, 194, 1007
DraI TTTAAA 3 cut(s) 403, 561, 1102
EaeI YGGCCR 1 cut(s) 647
Eco24I GRGCYC 1 cut(s) 379
EcoRII CCWGG 1 cut(s) 514
EcoT22I ATGCAT 2 cut(s) 256, 1072
EcoT38I GRGCYC 1 cut(s) 379
FaeI CATG 8 cut(s) 69, 449, 628, 754, 894, 925, 1070, 1074
FaqI GGGAC 1 cut(s) 296
FatI CATG 8 cut(s) 65, 445, 624, 750, 890, 921, 1066, 1070
FauI CCCGC 1 cut(s) 133
FauNDI CATATG 1 cut(s) 1046
Fnu4HI GCNGC 3 cut(s) 650, 884, 945
FokI GGATG 1 cut(s) 1065
FriOI GRGCYC 1 cut(s) 379
Fsp4HI GCNGC 3 cut(s) 650, 884, 945
FspBI CTAG 1 cut(s) 948
GluI GCNGC 3 cut(s) 650, 884, 945
GsuI CTGGAG 1 cut(s) 166
HaeIII GGCC 1 cut(s) 649
HapII CCGG 4 cut(s) 306, 591, 614, 955
Hin1II CATG 8 cut(s) 69, 449, 628, 754, 894, 925, 1070, 1074
HincII GTYRAC 1 cut(s) 856
HindII GTYRAC 1 cut(s) 856
HindIII AAGCTT 1 cut(s) 555
HinfI GANTC 4 cut(s) 10, 309, 331, 812
HpaI GTTAAC 1 cut(s) 856
HpaII CCGG 4 cut(s) 306, 591, 614, 955
HphI GGTGA 1 cut(s) 821
Hpy166II GTNNAC 1 cut(s) 856
Hpy188I TCNGA 3 cut(s) 88, 724, 907
Hpy188III TCNNGA 4 cut(s) 183, 335, 526, 641
Hpy8I GTNNAC 1 cut(s) 856
Hpy99I CGWCG 2 cut(s) 92, 820
HpyAV CCTTC 5 cut(s) 35, 281, 439, 706, 719
HpyCH4III ACNGT 3 cut(s) 96, 152, 346
HpyCH4V TGCA 5 cut(s) 99, 254, 458, 734, 1070
HpyF3I CTNAG 2 cut(s) 106, 906
Hsp92II CATG 8 cut(s) 69, 449, 628, 754, 894, 925, 1070, 1074
KspAI GTTAAC 1 cut(s) 856
Kzo9I GATC 3 cut(s) 109, 194, 1007
LmnI GCTCC 2 cut(s) 89, 185
Lsp1109I GCAGC 2 cut(s) 895, 931
LweI GCATC 1 cut(s) 372
MaeI CTAG 1 cut(s) 948
MaeIII GTNAC 4 cut(s) 319, 346, 526, 1055
MalI GATC 3 cut(s) 111, 196, 1009
MboI GATC 3 cut(s) 109, 194, 1007
MboII GAAGA 2 cut(s) 16, 350
MhlI GDGCHC 2 cut(s) 119, 379
MluCI AATT 4 cut(s) 212, 896, 901, 964
MlyI GAGTC 2 cut(s) 4, 318
MmeI TCCRAC 1 cut(s) 111
MnlI CCTC 8 cut(s) 130, 324, 535, 800, 831, 901, 992, 998
Mph1103I ATGCAT 2 cut(s) 256, 1072
MroXI GAANNNNTTC 1 cut(s) 796
MseI TTAA 8 cut(s) 360, 402, 482, 560, 855, 879, 1101, 1109
MslI CAYNNNNRTG 2 cut(s) 739, 1071
MspA1I CMGCKG 2 cut(s) 470, 652
MspI CCGG 4 cut(s) 306, 591, 614, 955
MspR9I CCNGG 1 cut(s) 516
MvaI CCWGG 1 cut(s) 516
NdeI CATATG 1 cut(s) 1046
NdeII GATC 3 cut(s) 109, 194, 1007
NlaIII CATG 8 cut(s) 69, 449, 628, 754, 894, 925, 1070, 1074
NlaIV GGNNCC 1 cut(s) 181
NmuCI GTSAC 2 cut(s) 346, 526
NsiI ATGCAT 2 cut(s) 256, 1072
NspI RCATGY 3 cut(s) 628, 925, 1074
PaeI GCATGC 1 cut(s) 925
PdmI GAANNNNTTC 1 cut(s) 796
PfeI GAWTC 2 cut(s) 331, 812
PflFI GACNNNGTC 1 cut(s) 344
PfoI TCCNGGA 1 cut(s) 514
PinAI ACCGGT 1 cut(s) 954
PkrI GCNGC 3 cut(s) 651, 885, 946
PleI GAGTC 2 cut(s) 4, 317
PpsI GAGTC 2 cut(s) 4, 317
Psp6I CCWGG 1 cut(s) 514
PspGI CCWGG 1 cut(s) 514
PspN4I GGNNCC 1 cut(s) 181
PsyI GACNNNGTC 1 cut(s) 344
PvuII CAGCTG 1 cut(s) 470
RseI CAYNNNNRTG 2 cut(s) 739, 1071
SaqAI TTAA 8 cut(s) 360, 402, 482, 560, 855, 879, 1101, 1109
SatI GCNGC 3 cut(s) 650, 884, 945
Sau3AI GATC 3 cut(s) 109, 194, 1007
SchI GAGTC 2 cut(s) 4, 318
ScrFI CCNGG 1 cut(s) 516
SduI GDGCHC 2 cut(s) 119, 379
SfaNI GCATC 1 cut(s) 372
SfcI CTRYAG 3 cut(s) 471, 1020, 1115
SmiMI CAYNNNNRTG 2 cut(s) 739, 1071
SphI GCATGC 1 cut(s) 925
Sse9I AATT 4 cut(s) 212, 896, 901, 964
SsiI CCGC 3 cut(s) 140, 650, 974
SspMI CTAG 1 cut(s) 948
StyD4I CCNGG 1 cut(s) 514
TaaI ACNGT 3 cut(s) 96, 152, 346
TaqI TCGA 2 cut(s) 119, 285
TasI AATT 4 cut(s) 212, 896, 901, 964
TauI GCSGC 1 cut(s) 652
TfiI GAWTC 2 cut(s) 331, 812
Tru1I TTAA 8 cut(s) 360, 402, 482, 560, 855, 879, 1101, 1109
Tru9I TTAA 8 cut(s) 360, 402, 482, 560, 855, 879, 1101, 1109
TscAI CASTG 5 cut(s) 101, 119, 349, 460, 746
TseFI GTSAC 2 cut(s) 346, 526
TseI GCWGC 2 cut(s) 883, 944
Tsp45I GTSAC 2 cut(s) 346, 526
TspDTI ATGAA 3 cut(s) 17, 54, 614
TspGWI ACGGA 2 cut(s) 105, 141
TspRI CASTG 5 cut(s) 101, 119, 349, 460, 746
Tth111I GACNNNGTC 1 cut(s) 344
XapI RAATTY 1 cut(s) 964
XceI RCATGY 3 cut(s) 628, 925, 1074
XmnI GAANNNNTTC 1 cut(s) 796
XspI CTAG 1 cut(s) 948
Zsp2I ATGCAT 2 cut(s) 256, 1072
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.