pycom09g00050

PI-PLC X domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0001750
Physical Location & Seq
Forward (+)
30023 .. 33495
3473 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g00050.1

Sequence Viewer

Length: 1137 bp
ATGAAGACTCTGCGTCACTTTTGGGTTGCAACTTGCAACATGAAGACTCTGCAGAACTTAGGGTTTACTCAGCAGAACCTCGTTTTGTTAGTCACGGTTTTCGCGGCGGTTTTAAGCATTGGACAGTGCAAGCTTTTGGACGAATGCTCGACGGACGCCGATTGTGAGGCCGGACTTCTCTGCGGGTCTTGCCCACAAGGTTTCTCAGGCACTAGATGTATAAGATCAGCCATTACAGATCAGTTCAAACTATTGAACAATTCTCTGCCGTTTAACAAGTATGCGTTTTTGACAACCCACAATGCCTTTGCAATTGACGGAGAGCCATCTCATACTGGAATTCCTCGCGTTACCTTCAACGATCAAGAAGAAACTGTCACTCAACAGCTCAGTGGTGGAGTTCGAGCCTTGATGCTGGATACGTATGATTTTAAGGATGATGTGTGGTTGTGCCATTCTTTTAAAGGAGAATGCCATGACTTTACTGCATTTGGACCAGCTATAGATACACTGAAGGAAGTTGAAGCATTTTTATCAGCCAACCCATCAGAAATTGTGACATTGATATTAGAGGACTACGTGGAAGCTCCAAATGGATTGACAAAGGTCTTCGAAGCTTCCGGGTTGAAGAAATATTGGTTTCCGGTGTCAAGCATGCCCAAAAATGGCCAGGACTGGCCACTAGTTAGTGACATGGTTGCCAAGAACCAAAGGCTACTTGTGTTGACTTCAAAAAAAGCCAAGGAACAATCTGAAGGAATCGCTTACCAGTGGAACTACATGGTTGAAAACCAATATGGGGATGATGGAATGAAGGAAGCTTGTTCAAATAGAGGAGAATCATCACCTCTTGATGACAAGACCAAATCTTTGGTTTTGGTGAATTATTTTGCTTCTGTTCCCATTAAGAAAGGCAGTTGCGTAGATAACTCTGGGAATCTGATTAACATGCTTTACACTTGTCATGGAGCTGCTGGCAACCGATGGGCTAACTTTGTTTCTGTTGATTTTTACAAGAGGAGTGATGGAGGAGGATCGTTTCAAGCTTTAGACAATCTCAATGGGGAGCTCTTGTGTGGATGTAACGATATACATGCATGTGTGCCAGGATCACCTCCAGGGTCTTGCAAGCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

379

Amino Acids

41.47

Weight (kDa)

5.23

Isoelectric Point (pI)

37.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC_cat PF26178 86 - 367 8.8e-145 PI-PLC-like catalytic domain
PI-PLC_X PF26146 117 - 304 7.9e-22 PI-PLC X
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 104, 348
AciI CCGC 3 cut(s) 104, 107, 183
AclWI GGATC 2 cut(s) 1042, 1117
AcoI YGGCCR 2 cut(s) 667, 677
AcsI RAATTY 1 cut(s) 339
AcuI CTGAAG 2 cut(s) 533, 774
AcyI GRCGYC 1 cut(s) 156
AfiI CCNNNNNNNGG 2 cut(s) 665, 799
AgsI TTSAA 9 cut(s) 247, 256, 358, 524, 628, 732, 788, 828, 1043
AhdI GACNNNNNGTC 1 cut(s) 12
AhlI ACTAGT 1 cut(s) 682
AjnI CCWGG 3 cut(s) 669, 1105, 1117
AluBI AGCT 9 cut(s) 133, 388, 500, 587, 617, 821, 971, 1046, 1069
AluI AGCT 9 cut(s) 133, 388, 500, 587, 617, 821, 971, 1046, 1069
Alw21I GWGCWC 1 cut(s) 1071
AlwI GGATC 2 cut(s) 1042, 1117
AoxI GGCC 3 cut(s) 168, 667, 677
ApeKI GCWGC 1 cut(s) 971
ApoI RAATTY 1 cut(s) 339
ArsI GACNNNNNNTTYG 2 cut(s) 853, 885
AspS9I GGNCC 1 cut(s) 494
AsuC2I CCSGG 1 cut(s) 622
AsuHPI GGTGA 3 cut(s) 837, 892, 1104
AsuII TTCGAA 1 cut(s) 612
AvaII GGWCC 1 cut(s) 494
BalI TGGCCA 2 cut(s) 669, 679
BanII GRGCYC 1 cut(s) 1071
BbsI GAAGAC 3 cut(s) 11, 50, 601
Bbv12I GWGCWC 1 cut(s) 1071
BbvI GCAGC 1 cut(s) 958
BccI CCATC 5 cut(s) 334, 553, 800, 978, 1019
BceAI ACGGC 1 cut(s) 253
BcgI CGANNNNNNTGC 2 cut(s) 1076, 1110
BciT130I CCWGG 3 cut(s) 671, 1107, 1119
BciVI GTATCC 1 cut(s) 412
BcnI CCSGG 1 cut(s) 622
BcuI ACTAGT 1 cut(s) 682
BfaI CTAG 2 cut(s) 213, 683
BfmI CTRYAG 2 cut(s) 50, 501
BfuI GTATCC 1 cut(s) 412
BisI GCNGC 2 cut(s) 105, 972
BlsI GCNGC 2 cut(s) 106, 973
Bme1390I CCNGG 4 cut(s) 622, 671, 1107, 1119
Bme18I GGWCC 1 cut(s) 494
BmeRI GACNNNNNGTC 1 cut(s) 12
BmgT120I GGNCC 1 cut(s) 494
BmrFI CCNGG 4 cut(s) 622, 671, 1107, 1119
BmsI GCATC 1 cut(s) 402
BoxI GACNNNNGTC 1 cut(s) 605
BpiI GAAGAC 3 cut(s) 11, 50, 601
BpmI CTGGAG 1 cut(s) 1101
Bpu14I TTCGAA 1 cut(s) 612
BpuMI CCSGG 1 cut(s) 622
BsaAI YACGTR 2 cut(s) 423, 580
BsaHI GRCGYC 1 cut(s) 156
BsaJI CCNNGG 2 cut(s) 741, 1118
BsaWI WCCGGW 1 cut(s) 643
BsaXI ACNNNNNCTCC 2 cut(s) 1058, 1088
Bsc4I CCNNNNNNNGG 2 cut(s) 665, 799
Bse1I ACTGG 3 cut(s) 340, 680, 769
BseBI CCWGG 3 cut(s) 671, 1107, 1119
BseDI CCNNGG 2 cut(s) 741, 1118
BseGI GGATG 3 cut(s) 442, 808, 1085
BseLI CCNNNNNNNGG 2 cut(s) 665, 799
BseMII CTCAG 3 cut(s) 83, 219, 403
BseNI ACTGG 3 cut(s) 340, 680, 769
BseRI GAGGAG 3 cut(s) 849, 1033, 1044
BseXI GCAGC 1 cut(s) 958
Bsh1236I CGCG 2 cut(s) 104, 348
BshFI GGCC 3 cut(s) 170, 669, 679
BsiHKAI GWGCWC 1 cut(s) 1071
BsiSI CCGG 3 cut(s) 171, 621, 644
BslI CCNNNNNNNGG 2 cut(s) 665, 799
BsmI GAATGC 2 cut(s) 149, 476
BsnI GGCC 3 cut(s) 170, 669, 679
Bsp119I TTCGAA 1 cut(s) 612
Bsp1286I GDGCHC 1 cut(s) 1071
Bsp143I GATC 5 cut(s) 224, 238, 361, 1034, 1109
BspACI CCGC 3 cut(s) 104, 107, 183
BspANI GGCC 3 cut(s) 170, 669, 679
BspCNI CTCAG 3 cut(s) 82, 218, 402
BspFNI CGCG 2 cut(s) 104, 348
BspMAI CTGCAG 1 cut(s) 54
BspPI GGATC 2 cut(s) 1042, 1117
BspT104I TTCGAA 1 cut(s) 612
BsrI ACTGG 3 cut(s) 340, 680, 769
BssECI CCNNGG 2 cut(s) 741, 1118
BssMI GATC 5 cut(s) 224, 238, 361, 1034, 1109
BssNI GRCGYC 1 cut(s) 156
BssT1I CCWWGG 1 cut(s) 741
Bst2UI CCWGG 3 cut(s) 671, 1107, 1119
Bst4CI ACNGT 3 cut(s) 97, 126, 376
BstACI GRCGYC 1 cut(s) 156
BstBAI YACGTR 2 cut(s) 423, 580
BstBI TTCGAA 1 cut(s) 612
BstC8I GCNNGC 4 cut(s) 131, 656, 976, 1130
BstDEI CTNAG 4 cut(s) 58, 69, 205, 389
BstF5I GGATG 3 cut(s) 442, 808, 1085
BstFNI CGCG 2 cut(s) 104, 348
BstKTI GATC 5 cut(s) 227, 241, 364, 1037, 1112
BstMBI GATC 5 cut(s) 224, 238, 361, 1034, 1109
BstMWI GCNNNNNNNGC 1 cut(s) 189
BstNI CCWGG 3 cut(s) 671, 1107, 1119
BstNSI RCATGY 4 cut(s) 658, 952, 1097, 1101
BstPAI GACNNNNGTC 1 cut(s) 605
BstSCI CCNGG 4 cut(s) 620, 669, 1105, 1117
BstSFI CTRYAG 2 cut(s) 50, 501
BstSNI TACGTA 1 cut(s) 423
BstUI CGCG 2 cut(s) 104, 348
BstV1I GCAGC 1 cut(s) 958
BstV2I GAAGAC 3 cut(s) 11, 50, 601
BstXI CCANNNNNNTGG 1 cut(s) 871
BsuI GTATCC 1 cut(s) 412
BsuRI GGCC 3 cut(s) 170, 669, 679
BtsCI GGATG 3 cut(s) 442, 808, 1085
BtsIMutI CAGTG 4 cut(s) 131, 397, 509, 776
Cac8I GCNNGC 4 cut(s) 131, 656, 976, 1130
Cfr13I GGNCC 1 cut(s) 494
CseI GACGC 2 cut(s) 2, 164
CviAII CATG 9 cut(s) 40, 476, 655, 694, 781, 949, 965, 1094, 1098
DdeI CTNAG 4 cut(s) 58, 69, 205, 389
DpnI GATC 5 cut(s) 226, 240, 363, 1036, 1111
DpnII GATC 5 cut(s) 224, 238, 361, 1034, 1109
DraI TTTAAA 1 cut(s) 463
DriI GACNNNNNGTC 1 cut(s) 12
EaeI YGGCCR 2 cut(s) 667, 677
Eam1105I GACNNNNNGTC 1 cut(s) 12
Ecl136II GAGCTC 1 cut(s) 1069
Eco105I TACGTA 1 cut(s) 423
Eco130I CCWWGG 1 cut(s) 741
Eco24I GRGCYC 1 cut(s) 1071
Eco47I GGWCC 1 cut(s) 494
Eco53kI GAGCTC 1 cut(s) 1069
Eco57I CTGAAG 2 cut(s) 533, 774
EcoICRI GAGCTC 1 cut(s) 1069
EcoRI GAATTC 1 cut(s) 339
EcoRII CCWGG 3 cut(s) 669, 1105, 1117
EcoT14I CCWWGG 1 cut(s) 741
EcoT22I ATGCAT 1 cut(s) 1099
EcoT38I GRGCYC 1 cut(s) 1071
ErhI CCWWGG 1 cut(s) 741
FaeI CATG 9 cut(s) 43, 479, 658, 697, 784, 952, 968, 1097, 1101
FatI CATG 9 cut(s) 39, 475, 654, 693, 780, 948, 964, 1093, 1097
FauI CCCGC 1 cut(s) 176
Fnu4HI GCNGC 2 cut(s) 105, 972
FokI GGATG 3 cut(s) 449, 815, 1092
FriOI GRGCYC 1 cut(s) 1071
Fsp4HI GCNGC 2 cut(s) 105, 972
FspBI CTAG 2 cut(s) 213, 683
GluI GCNGC 2 cut(s) 105, 972
GsuI CTGGAG 1 cut(s) 1101
HaeIII GGCC 3 cut(s) 170, 669, 679
HapII CCGG 3 cut(s) 171, 621, 644
HgaI GACGC 2 cut(s) 2, 164
Hin1I GRCGYC 1 cut(s) 156
Hin1II CATG 9 cut(s) 43, 479, 658, 697, 784, 952, 968, 1097, 1101
HincII GTYRAC 1 cut(s) 726
HindII GTYRAC 1 cut(s) 726
HindIII AAGCTT 4 cut(s) 131, 615, 819, 1044
HinfI GANTC 5 cut(s) 7, 46, 759, 839, 937
HpaII CCGG 3 cut(s) 171, 621, 644
HphI GGTGA 3 cut(s) 837, 892, 1104
Hpy166II GTNNAC 2 cut(s) 66, 726
Hpy188I TCNGA 3 cut(s) 550, 754, 942
Hpy188III TCNNGA 2 cut(s) 365, 851
Hpy8I GTNNAC 2 cut(s) 66, 726
Hpy99I CGWCG 1 cut(s) 154
HpyAV CCTTC 4 cut(s) 364, 508, 749, 808
HpyCH4III ACNGT 3 cut(s) 97, 126, 376
HpyCH4IV ACGT 2 cut(s) 422, 579
HpyCH4V TGCA 8 cut(s) 29, 36, 52, 129, 311, 488, 1097, 1128
HpyF10VI GCNNNNNNNGC 1 cut(s) 189
HpyF3I CTNAG 4 cut(s) 58, 69, 205, 389
HpySE526I ACGT 2 cut(s) 422, 579
Hsp92I GRCGYC 1 cut(s) 156
Hsp92II CATG 9 cut(s) 43, 479, 658, 697, 784, 952, 968, 1097, 1101
Kzo9I GATC 5 cut(s) 224, 238, 361, 1034, 1109
LmnI GCTCC 3 cut(s) 592, 968, 1066
Lsp1109I GCAGC 1 cut(s) 958
LweI GCATC 1 cut(s) 402
MaeI CTAG 2 cut(s) 213, 683
MaeII ACGT 2 cut(s) 422, 579
MaeIII GTNAC 7 cut(s) 14, 91, 349, 376, 556, 689, 1082
MalI GATC 5 cut(s) 226, 240, 363, 1036, 1111
MboI GATC 5 cut(s) 224, 238, 361, 1034, 1109
MboII GAAGA 5 cut(s) 16, 55, 380, 601, 640
MfeI CAATTG 1 cut(s) 312
MhlI GDGCHC 1 cut(s) 1071
MlsI TGGCCA 2 cut(s) 669, 679
MluCI AATT 5 cut(s) 259, 312, 339, 552, 883
MluNI TGGCCA 2 cut(s) 669, 679
MlyI GAGTC 1 cut(s) 40
Mox20I TGGCCA 2 cut(s) 669, 679
Mph1103I ATGCAT 1 cut(s) 1099
MscI TGGCCA 2 cut(s) 669, 679
MseI TTAA 6 cut(s) 113, 273, 432, 462, 906, 945
MslI CAYNNNNRTG 1 cut(s) 1098
Msp20I TGGCCA 2 cut(s) 669, 679
MspI CCGG 3 cut(s) 171, 621, 644
MspR9I CCNGG 4 cut(s) 622, 671, 1107, 1119
MunI CAATTG 1 cut(s) 312
Mva1269I GAATGC 2 cut(s) 149, 476
MvaI CCWGG 3 cut(s) 671, 1107, 1119
MvnI CGCG 2 cut(s) 104, 348
MwoI GCNNNNNNNGC 1 cut(s) 189
NciI CCSGG 1 cut(s) 622
NdeII GATC 5 cut(s) 224, 238, 361, 1034, 1109
NlaIII CATG 9 cut(s) 43, 479, 658, 697, 784, 952, 968, 1097, 1101
NmuCI GTSAC 5 cut(s) 14, 91, 376, 556, 689
NsiI ATGCAT 1 cut(s) 1099
NspI RCATGY 4 cut(s) 658, 952, 1097, 1101
NspV TTCGAA 1 cut(s) 612
PaeI GCATGC 1 cut(s) 658
PctI GAATGC 2 cut(s) 149, 476
PfeI GAWTC 3 cut(s) 759, 839, 937
PkrI GCNGC 2 cut(s) 106, 973
PleI GAGTC 1 cut(s) 40
PpsI GAGTC 1 cut(s) 40
Ppu21I YACGTR 2 cut(s) 423, 580
PshAI GACNNNNGTC 1 cut(s) 605
Psp124BI GAGCTC 1 cut(s) 1071
Psp6I CCWGG 3 cut(s) 669, 1105, 1117
PspGI CCWGG 3 cut(s) 669, 1105, 1117
PspPI GGNCC 1 cut(s) 494
PstI CTGCAG 1 cut(s) 54
RseI CAYNNNNRTG 1 cut(s) 1098
SacI GAGCTC 1 cut(s) 1071
SaqAI TTAA 6 cut(s) 113, 273, 432, 462, 906, 945
SatI GCNGC 2 cut(s) 105, 972
Sau3AI GATC 5 cut(s) 224, 238, 361, 1034, 1109
Sau96I GGNCC 1 cut(s) 494
SchI GAGTC 1 cut(s) 40
ScrFI CCNGG 4 cut(s) 622, 671, 1107, 1119
SduI GDGCHC 1 cut(s) 1071
SfaNI GCATC 1 cut(s) 402
SfcI CTRYAG 2 cut(s) 50, 501
SfuI TTCGAA 1 cut(s) 612
SinI GGWCC 1 cut(s) 494
SmiMI CAYNNNNRTG 1 cut(s) 1098
SnaBI TACGTA 1 cut(s) 423
SpeI ACTAGT 1 cut(s) 682
SphI GCATGC 1 cut(s) 658
Sse9I AATT 5 cut(s) 259, 312, 339, 552, 883
SsiI CCGC 3 cut(s) 104, 107, 183
SspI AATATT 1 cut(s) 635
SspMI CTAG 2 cut(s) 213, 683
SstI GAGCTC 1 cut(s) 1071
StyD4I CCNGG 4 cut(s) 620, 669, 1105, 1117
StyI CCWWGG 1 cut(s) 741
TaaI ACNGT 3 cut(s) 97, 126, 376
TaiI ACGT 2 cut(s) 425, 582
TaqI TCGA 3 cut(s) 149, 403, 612
TasI AATT 5 cut(s) 259, 312, 339, 552, 883
TauI GCSGC 1 cut(s) 107
TfiI GAWTC 3 cut(s) 759, 839, 937
Tru1I TTAA 6 cut(s) 113, 273, 432, 462, 906, 945
Tru9I TTAA 6 cut(s) 113, 273, 432, 462, 906, 945
TscAI CASTG 4 cut(s) 131, 397, 516, 776
TseFI GTSAC 5 cut(s) 14, 91, 376, 556, 689
TseI GCWGC 1 cut(s) 971
Tsp45I GTSAC 5 cut(s) 14, 91, 376, 556, 689
TspDTI ATGAA 3 cut(s) 17, 56, 827
TspGWI ACGGA 2 cut(s) 167, 333
TspRI CASTG 4 cut(s) 131, 397, 516, 776
VpaK11BI GGWCC 1 cut(s) 494
XapI RAATTY 1 cut(s) 339
XceI RCATGY 4 cut(s) 658, 952, 1097, 1101
XspI CTAG 2 cut(s) 213, 683
Zsp2I ATGCAT 1 cut(s) 1099
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.