RchiOBHm_Chr2g0165081

PI-PLC X domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001750
Physical Location & Seq
Forward (+)
80112757 .. 80115224
2468 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53305

Sequence Viewer

Length: 957 bp
ATGTGTGAGATCGACCACCACCAACCAATTCCAGCTTTTGACATGGAATATCATCTTATCATTTGCTACACAAGCTTGAATAGTTCTCTACCATTCAACAAATATGCATTTTTGACAACCCACAATGCTTTTGCCATCGAAGGAGAGCCATCTCATACCGGAGTCCCTCGTGTTACCTTGACGAATCAAGAAGACACTGTCACTCAACAACTTAACAATGGAGTTAGAGCCCTGATGCTTGATACCTATGATTTTAAAGGAGATGTCTGGTTGTGCCATTCCTTCAAAGGACAATGCCATGACTACACTGCATTTGAGCCAGCTATAGATACATTAAACGAAATCCAAGCATTTTTATCAGCAAATCCAGGAGAAATCGTGACATTGATATTAGAGGACTATGTTGAAGCTTTAAATGGACTGACAAATGTTTTCAAATCTGCCGGATTGATGAAATACTGGTTTCCGGTATCAAACATGCCCAAAAGTGGTCAGGATTGGCCGCTGGTTAGCGATATGGTTGCCAAGAACCAAAGGCTACTTGTATTCACTTCAAAACAAGAGAAGGAACAATCCGAAGGGATTGCATACCAGTGGAACTACATGGTTGAAAACGAGTCAAGAAAATTTGTAGATGGAGATGATGGAATGGAAGCGGGAAGCTGTTCAAACAGAGGTGAATCGTCGCCTCTAAATGACAAGACTAAATCATTGGTGCTGGTTAACTATTTTGGGTCATTTCCCATTAAGCAGCTCTCATGTCAATTCAATTCTGAGGATTTGGTTAGCATGCTTTACACTTGCTATTGTGCTGCTGGAAACCGGTGGGCGAATTTTGTTGCGGTTGATTTTTACAAATGGAGGGGAGGATCATTTCAAGCTACAGACACTCTCAATGGAGAACTCATATGTGGATGTAACGATGTCCATGCATGTGTGGTAAGCTATGAAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

318

Amino Acids

35.79

Weight (kDa)

4.72

Isoelectric Point (pI)

34.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC_cat PF26178 24 - 312 3.7e-139 PI-PLC-like catalytic domain
PI-PLC_X PF26146 57 - 252 4.8e-21 PI-PLC X
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 503, 656, 842
AclWI GGATC 1 cut(s) 877
AcoI YGGCCR 1 cut(s) 500
AcsI RAATTY 2 cut(s) 626, 832
AfiI CCNNNNNNNGG 1 cut(s) 488
AgeI ACCGGT 1 cut(s) 822
AjnI CCWGG 1 cut(s) 367
AluBI AGCT 8 cut(s) 35, 75, 323, 410, 663, 754, 881, 945
AluI AGCT 8 cut(s) 35, 75, 323, 410, 663, 754, 881, 945
AlwI GGATC 1 cut(s) 877
AoxI GGCC 1 cut(s) 500
ApeKI GCWGC 2 cut(s) 751, 812
ApoI RAATTY 2 cut(s) 626, 832
AsiGI ACCGGT 1 cut(s) 822
Asp700I GAANNNNTTC 1 cut(s) 664
AsuHPI GGTGA 1 cut(s) 689
BanII GRGCYC 1 cut(s) 232
BauI CACGAG 1 cut(s) 168
BbsI GAAGAC 1 cut(s) 198
BbvI GCAGC 2 cut(s) 763, 799
BccI CCATC 4 cut(s) 143, 157, 629, 638
BcgI CGANNNNNNTGC 6 cut(s) 503, 537, 566, 600, 911, 945
BciT130I CCWGG 1 cut(s) 369
BfmI CTRYAG 2 cut(s) 324, 882
BisI GCNGC 3 cut(s) 503, 752, 813
BlsI GCNGC 3 cut(s) 504, 753, 814
Bme1390I CCNGG 1 cut(s) 369
BmrFI CCNGG 1 cut(s) 369
BmsI GCATC 1 cut(s) 225
BpiI GAAGAC 1 cut(s) 198
BsaWI WCCGGW 3 cut(s) 158, 466, 822
BsaXI ACNNNNNCTCC 2 cut(s) 363, 393
Bsc4I CCNNNNNNNGG 1 cut(s) 488
Bse118I RCCGGY 1 cut(s) 822
Bse1I ACTGG 2 cut(s) 464, 592
BseBI CCWGG 1 cut(s) 369
BseGI GGATG 1 cut(s) 920
BseLI CCNNNNNNNGG 1 cut(s) 488
BseMII CTCAG 1 cut(s) 765
BseNI ACTGG 2 cut(s) 464, 592
BseXI GCAGC 2 cut(s) 763, 799
BshFI GGCC 1 cut(s) 502
BshTI ACCGGT 1 cut(s) 822
BsiSI CCGG 4 cut(s) 159, 444, 467, 823
BslFI GGGAC 1 cut(s) 149
BslI CCNNNNNNNGG 1 cut(s) 488
BsmFI GGGAC 1 cut(s) 149
BsnI GGCC 1 cut(s) 502
Bsp1286I GDGCHC 1 cut(s) 232
Bsp143I GATC 2 cut(s) 9, 869
BspACI CCGC 3 cut(s) 503, 656, 842
BspANI GGCC 1 cut(s) 502
BspCNI CTCAG 1 cut(s) 766
BspPI GGATC 1 cut(s) 877
BsrFI RCCGGY 1 cut(s) 822
BsrI ACTGG 2 cut(s) 464, 592
BssAI RCCGGY 1 cut(s) 822
BssMI GATC 2 cut(s) 9, 869
BssSI CACGAG 1 cut(s) 168
Bst2BI CACGAG 1 cut(s) 168
Bst2UI CCWGG 1 cut(s) 369
Bst4CI ACNGT 1 cut(s) 199
BstC8I GCNNGC 2 cut(s) 321, 791
BstDEI CTNAG 1 cut(s) 774
BstF5I GGATG 1 cut(s) 920
BstKTI GATC 2 cut(s) 12, 872
BstMBI GATC 2 cut(s) 9, 869
BstMWI GCNNNNNNNGC 1 cut(s) 72
BstNI CCWGG 1 cut(s) 369
BstNSI RCATGY 3 cut(s) 481, 793, 936
BstSCI CCNGG 1 cut(s) 367
BstSFI CTRYAG 2 cut(s) 324, 882
BstV1I GCAGC 2 cut(s) 763, 799
BstV2I GAAGAC 1 cut(s) 198
BsuRI GGCC 1 cut(s) 502
BtsCI GGATG 1 cut(s) 920
BtsI GCAGTG 1 cut(s) 306
BtsIMutI CAGTG 3 cut(s) 195, 306, 599
Cac8I GCNNGC 2 cut(s) 321, 791
Cfr10I RCCGGY 1 cut(s) 822
CspAI ACCGGT 1 cut(s) 822
CviAII CATG 8 cut(s) 43, 299, 478, 604, 759, 790, 929, 933
DdeI CTNAG 1 cut(s) 774
DpnI GATC 2 cut(s) 11, 871
DpnII GATC 2 cut(s) 9, 869
DraI TTTAAA 2 cut(s) 256, 414
EaeI YGGCCR 1 cut(s) 500
Eco24I GRGCYC 1 cut(s) 232
EcoRII CCWGG 1 cut(s) 367
EcoT22I ATGCAT 2 cut(s) 109, 934
EcoT38I GRGCYC 1 cut(s) 232
FaeI CATG 8 cut(s) 46, 302, 481, 607, 762, 793, 932, 936
FaqI GGGAC 1 cut(s) 149
FatI CATG 8 cut(s) 42, 298, 477, 603, 758, 789, 928, 932
FauI CCCGC 1 cut(s) 649
FauNDI CATATG 1 cut(s) 908
Fnu4HI GCNGC 3 cut(s) 503, 752, 813
FokI GGATG 1 cut(s) 927
FriOI GRGCYC 1 cut(s) 232
Fsp4HI GCNGC 3 cut(s) 503, 752, 813
GluI GCNGC 3 cut(s) 503, 752, 813
HaeIII GGCC 1 cut(s) 502
HapII CCGG 4 cut(s) 159, 444, 467, 823
Hin1II CATG 8 cut(s) 46, 302, 481, 607, 762, 793, 932, 936
HincII GTYRAC 1 cut(s) 724
HindII GTYRAC 1 cut(s) 724
HindIII AAGCTT 2 cut(s) 73, 408
HinfI GANTC 4 cut(s) 162, 184, 617, 680
HpaI GTTAAC 1 cut(s) 724
HpaII CCGG 4 cut(s) 159, 444, 467, 823
HphI GGTGA 1 cut(s) 689
Hpy166II GTNNAC 1 cut(s) 724
Hpy188I TCNGA 2 cut(s) 577, 775
Hpy188III TCNNGA 4 cut(s) 188, 379, 494, 621
Hpy8I GTNNAC 1 cut(s) 724
Hpy99I CGWCG 1 cut(s) 688
HpyAV CCTTC 4 cut(s) 134, 292, 559, 572
HpyCH4III ACNGT 1 cut(s) 199
HpyCH4V TGCA 4 cut(s) 107, 311, 587, 932
HpyF10VI GCNNNNNNNGC 1 cut(s) 72
HpyF3I CTNAG 1 cut(s) 774
Hsp92II CATG 8 cut(s) 46, 302, 481, 607, 762, 793, 932, 936
KspAI GTTAAC 1 cut(s) 724
Kzo9I GATC 2 cut(s) 9, 869
Lsp1109I GCAGC 2 cut(s) 763, 799
LweI GCATC 1 cut(s) 225
MaeIII GTNAC 4 cut(s) 172, 199, 379, 917
MalI GATC 2 cut(s) 11, 871
MboI GATC 2 cut(s) 9, 869
MboII GAAGA 1 cut(s) 203
MhlI GDGCHC 1 cut(s) 232
MluCI AATT 5 cut(s) 27, 626, 764, 769, 832
MlyI GAGTC 2 cut(s) 171, 626
MnlI CCTC 7 cut(s) 177, 388, 668, 699, 769, 855, 860
Mph1103I ATGCAT 2 cut(s) 109, 934
MroXI GAANNNNTTC 1 cut(s) 664
MseI TTAA 6 cut(s) 213, 255, 335, 413, 723, 747
MslI CAYNNNNRTG 2 cut(s) 592, 933
MspA1I CMGCKG 1 cut(s) 505
MspI CCGG 4 cut(s) 159, 444, 467, 823
MspR9I CCNGG 1 cut(s) 369
MvaI CCWGG 1 cut(s) 369
MwoI GCNNNNNNNGC 1 cut(s) 72
NdeI CATATG 1 cut(s) 908
NdeII GATC 2 cut(s) 9, 869
NlaIII CATG 8 cut(s) 46, 302, 481, 607, 762, 793, 932, 936
NmuCI GTSAC 2 cut(s) 199, 379
NsiI ATGCAT 2 cut(s) 109, 934
NspI RCATGY 3 cut(s) 481, 793, 936
PaeI GCATGC 1 cut(s) 793
PdmI GAANNNNTTC 1 cut(s) 664
PfeI GAWTC 2 cut(s) 184, 680
PflFI GACNNNGTC 1 cut(s) 197
PfoI TCCNGGA 1 cut(s) 367
PinAI ACCGGT 1 cut(s) 822
PkrI GCNGC 3 cut(s) 504, 753, 814
PleI GAGTC 2 cut(s) 170, 625
PpsI GAGTC 2 cut(s) 170, 625
Psp6I CCWGG 1 cut(s) 367
PspGI CCWGG 1 cut(s) 367
PsyI GACNNNGTC 1 cut(s) 197
RseI CAYNNNNRTG 2 cut(s) 592, 933
SaqAI TTAA 6 cut(s) 213, 255, 335, 413, 723, 747
SatI GCNGC 3 cut(s) 503, 752, 813
Sau3AI GATC 2 cut(s) 9, 869
SchI GAGTC 2 cut(s) 171, 626
ScrFI CCNGG 1 cut(s) 369
SduI GDGCHC 1 cut(s) 232
SfaNI GCATC 1 cut(s) 225
SfcI CTRYAG 2 cut(s) 324, 882
SmiMI CAYNNNNRTG 2 cut(s) 592, 933
SphI GCATGC 1 cut(s) 793
Sse9I AATT 5 cut(s) 27, 626, 764, 769, 832
SsiI CCGC 3 cut(s) 503, 656, 842
StyD4I CCNGG 1 cut(s) 367
TaaI ACNGT 1 cut(s) 199
TaqI TCGA 2 cut(s) 12, 138
TasI AATT 5 cut(s) 27, 626, 764, 769, 832
TauI GCSGC 1 cut(s) 505
TfiI GAWTC 2 cut(s) 184, 680
Tru1I TTAA 6 cut(s) 213, 255, 335, 413, 723, 747
Tru9I TTAA 6 cut(s) 213, 255, 335, 413, 723, 747
TscAI CASTG 3 cut(s) 202, 313, 599
TseFI GTSAC 2 cut(s) 199, 379
TseI GCWGC 2 cut(s) 751, 812
Tsp45I GTSAC 2 cut(s) 199, 379
TspDTI ATGAA 1 cut(s) 467
TspRI CASTG 3 cut(s) 202, 313, 599
Tth111I GACNNNGTC 1 cut(s) 197
XapI RAATTY 2 cut(s) 626, 832
XceI RCATGY 3 cut(s) 481, 793, 936
XmnI GAANNNNTTC 1 cut(s) 664
Zsp2I ATGCAT 2 cut(s) 109, 934
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.