Rh1DG025300

PI-PLC X domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0001750
Physical Location & Seq
Forward (+)
3980289 .. 3982803
2515 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG025300.1

Sequence Viewer

Length: 1107 bp
ATGAAGACGACTCGGCACAGCTTCCTTCTGGTTCTAGTTCTGGTTATGGCTTTTGCTGTGTTTTCATGTGTCGTCATAGCTTGCTCCGATGGACAGTGCAAGCTCTTAGATCAGTGCTCGACGGACGGAGATTGTGAGGCGGGGCTTTACTGTTTCGGTTGCCCTTCGGAGTTTTCTGGCTCCAGATGTGTGAGATCAACCACCACCAACCAATTCCAGCTTTTGAATAATTCTCTACCATTCAACAAATATGCATTTTTGACAACCCACAATGCTTTTGCTATCGAAGGAGAGCCATCTCATACCGGAGTCCCTCGTATTACCTTCACGAATCAAGAAGATACTGTCACTCAACAACTTAACAATGGAGTTAGAGCTCTAATGCTTGATACCTATGATTTTAAAGGAGATGTCTGGTTGTGCCATTCCTTCAAAGGAAAATGCCATGACTACACTGCATTTGAGCCAGCTATAGATACATTGAATGAAATCCAAGCATTTTTATCAGCAAACCCAGGAGAAATTGTGACATTGATATTAGAGGACTATGTTGAAGCTCCAAACGGATTGACAAATGTTTTCAAAGCTGCCGGATTGATGAAATACTGGTTTCCGGTATCAAACATGCCCAAAAGTGGTCAGGATTGGCCGCTGGTTAGCGATATGGTCACTAAGAACCAAAGGCTACTTGTATTCACTTCAAAACAAGAGAAGGAACAATCCGAAGGGATTGCATACCAGTGGAACTATATGGTTGAAAACCAGTATGGAAATGATGGAATGAAAGCGGGAAGCTGTTCAAACAGAGCTGAATCGTCTCCTCTAAATGACAAGACTAAATCATTGGTGTTGGTTAACTATTTTGGGTCAGTTCCCATTAAGCAGCTCTCATGTCAATTCAATTCTGAGGATTTGGTTAGCATGCTTAACACTTGCTATGGTGCTGCTGGAAACCGATGGGCAAATTTTGTTGCGGTTGATTTTTACAAGAGGAGTGGAGGAGGGGGATCATTTCAAGCTACAGACACTCTCAATGGAGAACTCATATGTGGATGTAACGATGTCCATGCATGTGTGCCAGGATCAACAACACCCTGCAAGGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

368

Amino Acids

40.49

Weight (kDa)

5.2

Isoelectric Point (pI)

35.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC_cat PF26178 75 - 358 8.1e-150 PI-PLC-like catalytic domain
PI-PLC_X PF26146 106 - 330 3.2e-24 PI-PLC X
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 140, 650, 788, 974
AclWI GGATC 2 cut(s) 1015, 1090
AcoI YGGCCR 1 cut(s) 647
AcsI RAATTY 1 cut(s) 964
AfiI CCNNNNNNNGG 1 cut(s) 635
AjnI CCWGG 2 cut(s) 514, 1078
Alw21I GWGCWC 2 cut(s) 119, 379
Alw26I GTCTC 1 cut(s) 822
AlwI GGATC 2 cut(s) 1015, 1090
AoxI GGCC 1 cut(s) 647
ApeKI GCWGC 3 cut(s) 587, 883, 944
ApoI RAATTY 1 cut(s) 964
Asp700I GAANNNNTTC 1 cut(s) 796
BanII GRGCYC 1 cut(s) 379
BbsI GAAGAC 1 cut(s) 11
Bbv12I GWGCWC 2 cut(s) 119, 379
BbvI GCAGC 3 cut(s) 574, 895, 931
BccI CCATC 4 cut(s) 83, 304, 770, 951
BcgI CGANNNNNNTGC 4 cut(s) 713, 747, 1049, 1083
BciT130I CCWGG 2 cut(s) 516, 1080
BcoDI GTCTC 1 cut(s) 822
BfaI CTAG 1 cut(s) 35
BfmI CTRYAG 2 cut(s) 471, 1020
BisI GCNGC 4 cut(s) 588, 650, 884, 945
BlsI GCNGC 4 cut(s) 589, 651, 885, 946
Bme1390I CCNGG 2 cut(s) 516, 1080
BmiI GGNNCC 1 cut(s) 181
BmrFI CCNGG 2 cut(s) 516, 1080
BpiI GAAGAC 1 cut(s) 11
BpmI CTGGAG 1 cut(s) 166
BsaJI CCNNGG 1 cut(s) 514
BsaWI WCCGGW 2 cut(s) 305, 613
BsaXI ACNNNNNCTCC 2 cut(s) 510, 540
Bsc4I CCNNNNNNNGG 1 cut(s) 635
Bse1I ACTGG 3 cut(s) 611, 739, 763
BseBI CCWGG 2 cut(s) 516, 1080
BseDI CCNNGG 1 cut(s) 514
BseGI GGATG 1 cut(s) 1058
BseLI CCNNNNNNNGG 1 cut(s) 635
BseMII CTCAG 1 cut(s) 897
BseNI ACTGG 3 cut(s) 611, 739, 763
BseRI GAGGAG 3 cut(s) 810, 1006, 1014
BseXI GCAGC 3 cut(s) 574, 895, 931
BshFI GGCC 1 cut(s) 649
BsiHKAI GWGCWC 2 cut(s) 119, 379
BsiSI CCGG 3 cut(s) 306, 591, 614
BslFI GGGAC 1 cut(s) 296
BslI CCNNNNNNNGG 1 cut(s) 635
BsmAI GTCTC 1 cut(s) 822
BsmBI CGTCTC 1 cut(s) 822
BsmFI GGGAC 1 cut(s) 296
BsnI GGCC 1 cut(s) 649
Bsp1286I GDGCHC 2 cut(s) 119, 379
Bsp143I GATC 4 cut(s) 109, 194, 1007, 1082
BspACI CCGC 4 cut(s) 140, 650, 788, 974
BspANI GGCC 1 cut(s) 649
BspCNI CTCAG 1 cut(s) 898
BspLI GGNNCC 1 cut(s) 181
BspPI GGATC 2 cut(s) 1015, 1090
BsrI ACTGG 3 cut(s) 611, 739, 763
BssECI CCNNGG 1 cut(s) 514
BssMI GATC 4 cut(s) 109, 194, 1007, 1082
Bst2UI CCWGG 2 cut(s) 516, 1080
Bst4CI ACNGT 3 cut(s) 96, 152, 346
BstC8I GCNNGC 4 cut(s) 82, 101, 468, 923
BstDEI CTNAG 3 cut(s) 106, 672, 906
BstF5I GGATG 1 cut(s) 1058
BstKTI GATC 4 cut(s) 112, 197, 1010, 1085
BstMAI GTCTC 1 cut(s) 822
BstMBI GATC 4 cut(s) 109, 194, 1007, 1082
BstNI CCWGG 2 cut(s) 516, 1080
BstNSI RCATGY 3 cut(s) 628, 925, 1074
BstSCI CCNGG 2 cut(s) 514, 1078
BstSFI CTRYAG 2 cut(s) 471, 1020
BstV1I GCAGC 3 cut(s) 574, 895, 931
BstV2I GAAGAC 1 cut(s) 11
BsuRI GGCC 1 cut(s) 649
BtsCI GGATG 1 cut(s) 1058
BtsI GCAGTG 1 cut(s) 453
BtsIMutI CAGTG 4 cut(s) 101, 119, 453, 746
Cac8I GCNNGC 4 cut(s) 82, 101, 468, 923
CspCI CAANNNNNGTGG 2 cut(s) 976, 1011
CviAII CATG 7 cut(s) 66, 446, 625, 891, 922, 1067, 1071
DdeI CTNAG 3 cut(s) 106, 672, 906
DpnI GATC 4 cut(s) 111, 196, 1009, 1084
DpnII GATC 4 cut(s) 109, 194, 1007, 1082
DraI TTTAAA 1 cut(s) 403
EaeI YGGCCR 1 cut(s) 647
Ecl136II GAGCTC 1 cut(s) 377
Eco24I GRGCYC 1 cut(s) 379
Eco53kI GAGCTC 1 cut(s) 377
EcoICRI GAGCTC 1 cut(s) 377
EcoRII CCWGG 2 cut(s) 514, 1078
EcoT22I ATGCAT 2 cut(s) 256, 1072
EcoT38I GRGCYC 1 cut(s) 379
Esp3I CGTCTC 1 cut(s) 822
FaeI CATG 7 cut(s) 69, 449, 628, 894, 925, 1070, 1074
FaqI GGGAC 1 cut(s) 296
FatI CATG 7 cut(s) 65, 445, 624, 890, 921, 1066, 1070
FauI CCCGC 2 cut(s) 133, 781
FauNDI CATATG 1 cut(s) 1046
Fnu4HI GCNGC 4 cut(s) 588, 650, 884, 945
FokI GGATG 1 cut(s) 1065
FriOI GRGCYC 1 cut(s) 379
Fsp4HI GCNGC 4 cut(s) 588, 650, 884, 945
FspBI CTAG 1 cut(s) 35
GluI GCNGC 4 cut(s) 588, 650, 884, 945
GsuI CTGGAG 1 cut(s) 166
HaeIII GGCC 1 cut(s) 649
HapII CCGG 3 cut(s) 306, 591, 614
Hin1II CATG 7 cut(s) 69, 449, 628, 894, 925, 1070, 1074
HincII GTYRAC 1 cut(s) 856
HindII GTYRAC 1 cut(s) 856
HinfI GANTC 4 cut(s) 10, 309, 331, 812
HpaI GTTAAC 1 cut(s) 856
HpaII CCGG 3 cut(s) 306, 591, 614
Hpy166II GTNNAC 1 cut(s) 856
Hpy188I TCNGA 4 cut(s) 88, 169, 724, 907
Hpy188III TCNNGA 4 cut(s) 183, 328, 335, 641
Hpy8I GTNNAC 1 cut(s) 856
Hpy99I CGWCG 1 cut(s) 124
HpyAV CCTTC 7 cut(s) 35, 174, 281, 334, 439, 706, 719
HpyCH4III ACNGT 3 cut(s) 96, 152, 346
HpyCH4V TGCA 6 cut(s) 99, 254, 458, 734, 1070, 1098
HpyF3I CTNAG 3 cut(s) 106, 672, 906
Hsp92II CATG 7 cut(s) 69, 449, 628, 894, 925, 1070, 1074
KspAI GTTAAC 1 cut(s) 856
Kzo9I GATC 4 cut(s) 109, 194, 1007, 1082
LmnI GCTCC 3 cut(s) 89, 185, 562
Lsp1109I GCAGC 3 cut(s) 574, 895, 931
MaeI CTAG 1 cut(s) 35
MaeIII GTNAC 4 cut(s) 346, 526, 667, 1055
MalI GATC 4 cut(s) 111, 196, 1009, 1084
MboI GATC 4 cut(s) 109, 194, 1007, 1082
MboII GAAGA 2 cut(s) 16, 350
MhlI GDGCHC 2 cut(s) 119, 379
MluCI AATT 6 cut(s) 212, 229, 522, 896, 901, 964
MlyI GAGTC 2 cut(s) 4, 318
MnlI CCTC 8 cut(s) 130, 324, 535, 831, 901, 984, 992, 995
Mph1103I ATGCAT 2 cut(s) 256, 1072
MroXI GAANNNNTTC 1 cut(s) 796
MseI TTAA 5 cut(s) 360, 402, 855, 879, 927
MslI CAYNNNNRTG 2 cut(s) 739, 1071
MspA1I CMGCKG 1 cut(s) 652
MspI CCGG 3 cut(s) 306, 591, 614
MspR9I CCNGG 2 cut(s) 516, 1080
MvaI CCWGG 2 cut(s) 516, 1080
NdeI CATATG 1 cut(s) 1046
NdeII GATC 4 cut(s) 109, 194, 1007, 1082
NlaIII CATG 7 cut(s) 69, 449, 628, 894, 925, 1070, 1074
NlaIV GGNNCC 1 cut(s) 181
NmuCI GTSAC 3 cut(s) 346, 526, 667
NsiI ATGCAT 2 cut(s) 256, 1072
NspI RCATGY 3 cut(s) 628, 925, 1074
PaeI GCATGC 1 cut(s) 925
PdmI GAANNNNTTC 1 cut(s) 796
PfeI GAWTC 2 cut(s) 331, 812
PkrI GCNGC 4 cut(s) 589, 651, 885, 946
PleI GAGTC 2 cut(s) 4, 317
PpsI GAGTC 2 cut(s) 4, 317
Psp124BI GAGCTC 1 cut(s) 379
Psp6I CCWGG 2 cut(s) 514, 1078
PspGI CCWGG 2 cut(s) 514, 1078
PspN4I GGNNCC 1 cut(s) 181
RseI CAYNNNNRTG 2 cut(s) 739, 1071
SacI GAGCTC 1 cut(s) 379
SaqAI TTAA 5 cut(s) 360, 402, 855, 879, 927
SatI GCNGC 4 cut(s) 588, 650, 884, 945
Sau3AI GATC 4 cut(s) 109, 194, 1007, 1082
SchI GAGTC 2 cut(s) 4, 318
ScrFI CCNGG 2 cut(s) 516, 1080
SduI GDGCHC 2 cut(s) 119, 379
SfcI CTRYAG 2 cut(s) 471, 1020
SmiMI CAYNNNNRTG 2 cut(s) 739, 1071
SphI GCATGC 1 cut(s) 925
Sse9I AATT 6 cut(s) 212, 229, 522, 896, 901, 964
SsiI CCGC 4 cut(s) 140, 650, 788, 974
SspMI CTAG 1 cut(s) 35
SstI GAGCTC 1 cut(s) 379
StyD4I CCNGG 2 cut(s) 514, 1078
TaaI ACNGT 3 cut(s) 96, 152, 346
TaqI TCGA 2 cut(s) 119, 285
TasI AATT 6 cut(s) 212, 229, 522, 896, 901, 964
TauI GCSGC 1 cut(s) 652
TfiI GAWTC 2 cut(s) 331, 812
Tru1I TTAA 5 cut(s) 360, 402, 855, 879, 927
Tru9I TTAA 5 cut(s) 360, 402, 855, 879, 927
TscAI CASTG 4 cut(s) 101, 119, 460, 746
TseFI GTSAC 3 cut(s) 346, 526, 667
TseI GCWGC 3 cut(s) 587, 883, 944
Tsp45I GTSAC 3 cut(s) 346, 526, 667
TspDTI ATGAA 5 cut(s) 17, 54, 501, 614, 797
TspGWI ACGGA 3 cut(s) 137, 141, 579
TspRI CASTG 4 cut(s) 101, 119, 460, 746
XapI RAATTY 1 cut(s) 964
XceI RCATGY 3 cut(s) 628, 925, 1074
XmnI GAANNNNTTC 1 cut(s) 796
XspI CTAG 1 cut(s) 35
Zsp2I ATGCAT 2 cut(s) 256, 1072
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.