RchiOBHm_Chr1g0319791

PI-PLC X domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001750
Physical Location & Seq
Reverse (-)
7439292 .. 7440732
1441 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55000

Sequence Viewer

Length: 999 bp
ATGAAGACATGGAATGCCACCTCATCATTTGCTACACAAACTTGGTACCTTTTTTCTAGTAGCATTGCTAATCTCAAATGCGATTTAAGTAAGATTTGCCTATTTGTGGTTCTACAGAATAATTCCCTACCATTCAACAAATATGCATTTTTGACAACCCACAATGCTTTTGCTATCGAAGGAGAGCCATCTCATACCAGAGTCCCTCGTGTTACCTTCACGAATCAAGAAGACACTGTCACTCAACAACTTAACAATGGAGTTAGAGCCCTAATGCTTGATACCTATGATTTTCAAGGAGATGTCTGGTTGTGCCATTCCTTCAAAGGAAAGTGCCATGACTACACTGCATTTGAGCCAACTATAGATACTTTGAATGAAATCCAAGCATTTTTATCAGCAAACCCAGGAGAAATTGTGACATTGATATTAGAGGACTATGTTGAAGCTCCAAACGGATTGACAAATGTTTTCAAAGCTGCTGGATTGATGAAATACTGGTTTCCGGTATCAAACATGCCCAAAAGTGGTCAGGATTGGCCGCTTGTTAGCGATATGGTCACTAGGAACCAAAGGCTACTTGTATTCAATTCAAAACAAGAGAAGGAACAATCCGAAGGGATTGCATACCAGTGGAACTACATGGTTGAAAACCAGTATGGAAATGATGGAATGAAAGCGGGAAGCTGTTCAAACAGAGCTGAATCGTCGCCTCAAAATGACAAGACTAAATCATTGGTGTTGGTTAACTATTTTAGGTCAGTTCCCATTAAGCAGCTCTCATGTCAATTCAATTATGAGGATTTGGTTAGCATGCTTAACACTTGCTATGGTGCTGCTGGAAATCGATGGGCAAATTTTGTTGCGGTTGATTTTTACAAGAGGAGTGGAGGAGGGGGATCATTTCAAGCTACAGACACTCTCAATGGAGAACTCATATGTGGATGTAACGATGTCCATGCATGTGTGCCAAGATCAACAACACCCTGCAAGGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

332

Amino Acids

37.22

Weight (kDa)

5.77

Isoelectric Point (pI)

38.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC_cat PF26178 39 - 322 1.3e-145 PI-PLC-like catalytic domain
PI-PLC_X PF26146 69 - 261 1.4e-24 PI-PLC X
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 45
AccB1I GGYRCC 1 cut(s) 45
AciI CCGC 3 cut(s) 542, 680, 866
AclWI GGATC 1 cut(s) 907
AcoI YGGCCR 1 cut(s) 539
AcsI RAATTY 1 cut(s) 856
AfaI GTAC 1 cut(s) 47
AfiI CCNNNNNNNGG 2 cut(s) 106, 527
AjnI CCWGG 1 cut(s) 406
AluBI AGCT 6 cut(s) 449, 479, 687, 701, 778, 911
AluI AGCT 6 cut(s) 449, 479, 687, 701, 778, 911
AlwI GGATC 1 cut(s) 907
AoxI GGCC 1 cut(s) 539
ApeKI GCWGC 3 cut(s) 479, 775, 836
ApoI RAATTY 1 cut(s) 856
Asp700I GAANNNNTTC 1 cut(s) 688
Asp718I GGTACC 1 cut(s) 45
BanI GGYRCC 1 cut(s) 45
BanII GRGCYC 1 cut(s) 271
BauI CACGAG 1 cut(s) 207
BbsI GAAGAC 2 cut(s) 11, 237
BbvI GCAGC 3 cut(s) 466, 787, 823
BccI CCATC 3 cut(s) 196, 662, 843
BcgI CGANNNNNNTGC 4 cut(s) 605, 639, 941, 975
BciT130I CCWGG 1 cut(s) 408
BfaI CTAG 2 cut(s) 57, 564
BfmI CTRYAG 3 cut(s) 113, 363, 912
BisI GCNGC 4 cut(s) 480, 542, 776, 837
BlsI GCNGC 4 cut(s) 481, 543, 777, 838
Bme1390I CCNGG 1 cut(s) 408
BmiI GGNNCC 2 cut(s) 47, 569
BmrFI CCNGG 1 cut(s) 408
BpiI GAAGAC 2 cut(s) 11, 237
Bsa29I ATCGAT 1 cut(s) 847
BsaJI CCNNGG 1 cut(s) 406
BsaWI WCCGGW 1 cut(s) 505
BsaXI ACNNNNNCTCC 2 cut(s) 402, 432
Bsc4I CCNNNNNNNGG 2 cut(s) 106, 527
Bse1I ACTGG 3 cut(s) 503, 631, 655
Bse3DI GCAATG 1 cut(s) 63
BseBI CCWGG 1 cut(s) 408
BseCI ATCGAT 1 cut(s) 847
BseDI CCNNGG 1 cut(s) 406
BseGI GGATG 1 cut(s) 950
BseLI CCNNNNNNNGG 2 cut(s) 106, 527
BseMI GCAATG 1 cut(s) 63
BseNI ACTGG 3 cut(s) 503, 631, 655
BseRI GAGGAG 2 cut(s) 898, 906
BseXI GCAGC 3 cut(s) 466, 787, 823
BshFI GGCC 1 cut(s) 541
BshNI GGYRCC 1 cut(s) 45
BshVI ATCGAT 1 cut(s) 847
BsiSI CCGG 1 cut(s) 506
BslFI GGGAC 1 cut(s) 188
BslI CCNNNNNNNGG 2 cut(s) 106, 527
BsmFI GGGAC 1 cut(s) 188
BsmI GAATGC 1 cut(s) 19
BsnI GGCC 1 cut(s) 541
Bsp1286I GDGCHC 1 cut(s) 271
Bsp143I GATC 2 cut(s) 899, 974
BspACI CCGC 3 cut(s) 542, 680, 866
BspANI GGCC 1 cut(s) 541
BspDI ATCGAT 1 cut(s) 847
BspLI GGNNCC 2 cut(s) 47, 569
BspPI GGATC 1 cut(s) 907
BspT107I GGYRCC 1 cut(s) 45
BsrDI GCAATG 1 cut(s) 63
BsrI ACTGG 3 cut(s) 503, 631, 655
BssECI CCNNGG 1 cut(s) 406
BssMI GATC 2 cut(s) 899, 974
BssSI CACGAG 1 cut(s) 207
Bst2BI CACGAG 1 cut(s) 207
Bst2UI CCWGG 1 cut(s) 408
Bst4CI ACNGT 1 cut(s) 238
BstC8I GCNNGC 1 cut(s) 815
BstF5I GGATG 1 cut(s) 950
BstKTI GATC 2 cut(s) 902, 977
BstMBI GATC 2 cut(s) 899, 974
BstNI CCWGG 1 cut(s) 408
BstNSI RCATGY 3 cut(s) 520, 817, 966
BstSCI CCNGG 1 cut(s) 406
BstSFI CTRYAG 3 cut(s) 113, 363, 912
BstV1I GCAGC 3 cut(s) 466, 787, 823
BstV2I GAAGAC 2 cut(s) 11, 237
Bsu15I ATCGAT 1 cut(s) 847
BsuRI GGCC 1 cut(s) 541
BsuTUI ATCGAT 1 cut(s) 847
BtsCI GGATG 1 cut(s) 950
BtsI GCAGTG 1 cut(s) 345
BtsIMutI CAGTG 3 cut(s) 234, 345, 638
Cac8I GCNNGC 1 cut(s) 815
ClaI ATCGAT 1 cut(s) 847
Csp6I GTAC 1 cut(s) 46
CspCI CAANNNNNGTGG 2 cut(s) 868, 903
CviAII CATG 8 cut(s) 9, 338, 517, 643, 783, 814, 959, 963
CviQI GTAC 1 cut(s) 46
DpnI GATC 2 cut(s) 901, 976
DpnII GATC 2 cut(s) 899, 974
EaeI YGGCCR 1 cut(s) 539
Eco24I GRGCYC 1 cut(s) 271
EcoRII CCWGG 1 cut(s) 406
EcoT22I ATGCAT 2 cut(s) 148, 964
EcoT38I GRGCYC 1 cut(s) 271
FaeI CATG 8 cut(s) 12, 341, 520, 646, 786, 817, 962, 966
FaqI GGGAC 1 cut(s) 188
FatI CATG 8 cut(s) 8, 337, 516, 642, 782, 813, 958, 962
FauI CCCGC 1 cut(s) 673
FauNDI CATATG 1 cut(s) 938
Fnu4HI GCNGC 4 cut(s) 480, 542, 776, 837
FokI GGATG 1 cut(s) 957
FriOI GRGCYC 1 cut(s) 271
Fsp4HI GCNGC 4 cut(s) 480, 542, 776, 837
FspBI CTAG 2 cut(s) 57, 564
GluI GCNGC 4 cut(s) 480, 542, 776, 837
HaeIII GGCC 1 cut(s) 541
HapII CCGG 1 cut(s) 506
Hin1II CATG 8 cut(s) 12, 341, 520, 646, 786, 817, 962, 966
HincII GTYRAC 1 cut(s) 748
HindII GTYRAC 1 cut(s) 748
HinfI GANTC 3 cut(s) 201, 223, 704
HpaI GTTAAC 1 cut(s) 748
HpaII CCGG 1 cut(s) 506
Hpy166II GTNNAC 1 cut(s) 748
Hpy188I TCNGA 1 cut(s) 616
Hpy188III TCNNGA 3 cut(s) 220, 227, 533
Hpy8I GTNNAC 1 cut(s) 748
Hpy99I CGWCG 1 cut(s) 712
HpyAV CCTTC 5 cut(s) 173, 226, 331, 598, 611
HpyCH4III ACNGT 1 cut(s) 238
HpyCH4V TGCA 5 cut(s) 146, 350, 626, 962, 990
Hsp92II CATG 8 cut(s) 12, 341, 520, 646, 786, 817, 962, 966
KpnI GGTACC 1 cut(s) 49
KspAI GTTAAC 1 cut(s) 748
Kzo9I GATC 2 cut(s) 899, 974
LmnI GCTCC 1 cut(s) 454
Lsp1109I GCAGC 3 cut(s) 466, 787, 823
MaeI CTAG 2 cut(s) 57, 564
MaeIII GTNAC 5 cut(s) 211, 238, 418, 559, 947
MalI GATC 2 cut(s) 901, 976
MboI GATC 2 cut(s) 899, 974
MboII GAAGA 2 cut(s) 16, 242
MhlI GDGCHC 1 cut(s) 271
MluCI AATT 6 cut(s) 121, 414, 589, 788, 793, 856
MlyI GAGTC 1 cut(s) 210
MnlI CCTC 8 cut(s) 31, 216, 427, 723, 793, 876, 884, 887
Mph1103I ATGCAT 2 cut(s) 148, 964
MroXI GAANNNNTTC 1 cut(s) 688
MseI TTAA 5 cut(s) 86, 252, 747, 771, 819
MslI CAYNNNNRTG 2 cut(s) 631, 963
MspI CCGG 1 cut(s) 506
MspR9I CCNGG 1 cut(s) 408
Mva1269I GAATGC 1 cut(s) 19
MvaI CCWGG 1 cut(s) 408
NdeI CATATG 1 cut(s) 938
NdeII GATC 2 cut(s) 899, 974
NlaIII CATG 8 cut(s) 12, 341, 520, 646, 786, 817, 962, 966
NlaIV GGNNCC 2 cut(s) 47, 569
NmuCI GTSAC 3 cut(s) 238, 418, 559
NsiI ATGCAT 2 cut(s) 148, 964
NspI RCATGY 3 cut(s) 520, 817, 966
PaeI GCATGC 1 cut(s) 817
PctI GAATGC 1 cut(s) 19
PdmI GAANNNNTTC 1 cut(s) 688
PfeI GAWTC 2 cut(s) 223, 704
PflFI GACNNNGTC 1 cut(s) 236
PkrI GCNGC 4 cut(s) 481, 543, 777, 838
PleI GAGTC 1 cut(s) 209
PpsI GAGTC 1 cut(s) 209
Psp6I CCWGG 1 cut(s) 406
PspGI CCWGG 1 cut(s) 406
PspN4I GGNNCC 2 cut(s) 47, 569
PsyI GACNNNGTC 1 cut(s) 236
RsaI GTAC 1 cut(s) 47
RsaNI GTAC 1 cut(s) 46
RseI CAYNNNNRTG 2 cut(s) 631, 963
SaqAI TTAA 5 cut(s) 86, 252, 747, 771, 819
SatI GCNGC 4 cut(s) 480, 542, 776, 837
Sau3AI GATC 2 cut(s) 899, 974
SchI GAGTC 1 cut(s) 210
ScrFI CCNGG 1 cut(s) 408
SduI GDGCHC 1 cut(s) 271
SfcI CTRYAG 3 cut(s) 113, 363, 912
SmiMI CAYNNNNRTG 2 cut(s) 631, 963
SphI GCATGC 1 cut(s) 817
Sse9I AATT 6 cut(s) 121, 414, 589, 788, 793, 856
SsiI CCGC 3 cut(s) 542, 680, 866
SspMI CTAG 2 cut(s) 57, 564
StyD4I CCNGG 1 cut(s) 406
TaaI ACNGT 1 cut(s) 238
TaqI TCGA 2 cut(s) 177, 847
TasI AATT 6 cut(s) 121, 414, 589, 788, 793, 856
TauI GCSGC 1 cut(s) 544
TfiI GAWTC 2 cut(s) 223, 704
Tru1I TTAA 5 cut(s) 86, 252, 747, 771, 819
Tru9I TTAA 5 cut(s) 86, 252, 747, 771, 819
TscAI CASTG 3 cut(s) 241, 352, 638
TseFI GTSAC 3 cut(s) 238, 418, 559
TseI GCWGC 3 cut(s) 479, 775, 836
Tsp45I GTSAC 3 cut(s) 238, 418, 559
TspDTI ATGAA 4 cut(s) 17, 393, 506, 689
TspGWI ACGGA 1 cut(s) 471
TspRI CASTG 3 cut(s) 241, 352, 638
Tth111I GACNNNGTC 1 cut(s) 236
XapI RAATTY 1 cut(s) 856
XceI RCATGY 3 cut(s) 520, 817, 966
XmnI GAANNNNTTC 1 cut(s) 688
XspI CTAG 2 cut(s) 57, 564
Zsp2I ATGCAT 2 cut(s) 148, 964
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.