MD17G1064700.v1.1

PI-PLC X domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001750
Physical Location & Seq
Forward (+)
5224983 .. 5228011
3029 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1064700.v1.1.491

Sequence Viewer

Length: 1098 bp
ATGAAGACTCGGCAGAACTTCGTTTTGTTACTCACGGTTGTCGCAGCGGTTTTTAGCGTCGCCAAAGCTTGCTCCAACGGACAGTGCAAGCTTTTGGACGAATGCTCGATGGATGCCGATTGTGAAGCCGGGCTTTACTGCGGATGTTGCCCACAAGGCTTCTCAGGCTCTAGATGTATAAGATCAGCCATTACAGATCAATTCAAGCTATTGAACAATTCTCTACCTTTCAACAAGTATGCGTTTTTGACAACCCACAATGCCTTTGCAATTGAGGGAGAGCCATCTCACACTGGAATTCCTCGCGTTACCTTCAACAATCAAGAAGATACTGTTACTCAACAACTAAATAATGGAGTTCGAGCCTTGATGCTCGATACATATGATTTTAAGGACGATGTGTGGTTGTGCCATTCTTTTAATGGGCAATGCCACGACTTTACTGCATTTGAACCAGCTATAGATACACTGAAGGAAGTTGAAGCATTTTTATCAGCCAACCCATCAGAAATTGTGACATTGATATTGGAGGACTATGTGGAAGCTCCAAATGGATTGACAAACGTCTTCAACGCTTCCGGGTTGAAGAACTATTGGTTTCCGGTGACAAGCATGCCCAAAAATGGCCAGGACTGGCCACTAGTTAGTGACATGGTTGCCAATAACCAGAGGCTACTTGTGTTTACTTCAAAAAGTGAGAAGGAACAATCCGAAGGAATCGCCTACCAGTGGAACTACATGGTTGAAAACCAATATGGGGATGATGGAATGAAGGAAGGAAGTTGTTCAAATAGAGGAGAATCATCACCTCTTGATGACAAAACTAAATCTTTGGTTTTGGTGAATTATTTTGCTTCTATTCCCTTTAAGCTAGAAAGTTGCGAAAATAACTCTGGGAATCTGATTAACATGCTTTACACTTGTCATGGTGCTGCTGCCAACCGATGGGCTAACTTTGTTGCTGTTGATTTTTACAAGAGGAGTGAAGGGGGAGGATCGTTTCAAGCTTTGGACAATCTCAATGGGGAGCTCTTGTGTGGATGTAACGATGTACATGCATGTGTGCCAGGATCACCTCCAGGGTCTTGCCAGCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

366

Amino Acids

40.16

Weight (kDa)

4.71

Isoelectric Point (pI)

37.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC_cat PF26178 72 - 354 4.2e-150 PI-PLC-like catalytic domain
PI-PLC_X PF26146 103 - 326 1.8e-23 PI-PLC X
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 945
AccII CGCG 1 cut(s) 306
AciI CCGC 2 cut(s) 47, 141
AclWI GGATC 2 cut(s) 1003, 1078
AcoI YGGCCR 2 cut(s) 625, 635
AcsI RAATTY 1 cut(s) 297
AcuI CTGAAG 1 cut(s) 491
AfaI GTAC 1 cut(s) 1053
AfiI CCNNNNNNNGG 4 cut(s) 623, 729, 757, 945
AhlI ACTAGT 1 cut(s) 640
AjnI CCWGG 3 cut(s) 627, 1066, 1078
AluBI AGCT 8 cut(s) 68, 91, 208, 458, 545, 871, 1007, 1030
AluI AGCT 8 cut(s) 68, 91, 208, 458, 545, 871, 1007, 1030
Alw21I GWGCWC 1 cut(s) 1032
AlwI GGATC 2 cut(s) 1003, 1078
AoxI GGCC 2 cut(s) 625, 635
ApeKI GCWGC 3 cut(s) 44, 932, 935
ApoI RAATTY 1 cut(s) 297
Asp700I GAANNNNTTC 1 cut(s) 784
AsuC2I CCSGG 2 cut(s) 130, 580
AsuHPI GGTGA 4 cut(s) 616, 798, 853, 1065
BalI TGGCCA 2 cut(s) 627, 637
BanII GRGCYC 1 cut(s) 1032
BbsI GAAGAC 2 cut(s) 11, 559
Bbv12I GWGCWC 1 cut(s) 1032
BbvI GCAGC 3 cut(s) 56, 919, 922
BccI CCATC 5 cut(s) 103, 292, 511, 758, 939
BcgI CGANNNNNNTGC 4 cut(s) 425, 459, 1037, 1071
BciT130I CCWGG 3 cut(s) 629, 1068, 1080
BcnI CCSGG 2 cut(s) 130, 580
BcuI ACTAGT 1 cut(s) 640
BfaI CTAG 3 cut(s) 171, 641, 872
BfmI CTRYAG 1 cut(s) 459
BglI GCCNNNNNGGC 1 cut(s) 156
BisI GCNGC 3 cut(s) 45, 933, 936
BlsI GCNGC 3 cut(s) 46, 934, 937
Bme1390I CCNGG 5 cut(s) 130, 580, 629, 1068, 1080
BmrFI CCNGG 5 cut(s) 130, 580, 629, 1068, 1080
BmsI GCATC 2 cut(s) 103, 360
BoxI GACNNNNGTC 1 cut(s) 563
BpiI GAAGAC 2 cut(s) 11, 559
BpmI CTGGAG 1 cut(s) 1062
BpuMI CCSGG 2 cut(s) 130, 580
BsaJI CCNNGG 1 cut(s) 1079
BsaWI WCCGGW 1 cut(s) 601
BsaXI ACNNNNNCTCC 2 cut(s) 1019, 1049
Bsc4I CCNNNNNNNGG 4 cut(s) 623, 729, 757, 945
Bse1I ACTGG 3 cut(s) 298, 638, 727
Bse3DI GCAATG 1 cut(s) 434
BseBI CCWGG 3 cut(s) 629, 1068, 1080
BseDI CCNNGG 1 cut(s) 1079
BseGI GGATG 4 cut(s) 118, 149, 766, 1046
BseLI CCNNNNNNNGG 4 cut(s) 623, 729, 757, 945
BseMI GCAATG 1 cut(s) 434
BseMII CTCAG 1 cut(s) 177
BseNI ACTGG 3 cut(s) 298, 638, 727
BseRI GAGGAG 2 cut(s) 810, 994
BseXI GCAGC 3 cut(s) 56, 919, 922
Bsh1236I CGCG 1 cut(s) 306
BshFI GGCC 2 cut(s) 627, 637
BsiHKAI GWGCWC 1 cut(s) 1032
BsiSI CCGG 3 cut(s) 129, 579, 602
BslI CCNNNNNNNGG 4 cut(s) 623, 729, 757, 945
BsmI GAATGC 1 cut(s) 107
BsnI GGCC 2 cut(s) 627, 637
Bsp1286I GDGCHC 1 cut(s) 1032
Bsp1407I TGTACA 1 cut(s) 1051
Bsp143I GATC 4 cut(s) 182, 196, 995, 1070
BspACI CCGC 2 cut(s) 47, 141
BspANI GGCC 2 cut(s) 627, 637
BspCNI CTCAG 1 cut(s) 176
BspFNI CGCG 1 cut(s) 306
BspPI GGATC 2 cut(s) 1003, 1078
BsrDI GCAATG 1 cut(s) 434
BsrGI TGTACA 1 cut(s) 1051
BsrI ACTGG 3 cut(s) 298, 638, 727
BssECI CCNNGG 1 cut(s) 1079
BssMI GATC 4 cut(s) 182, 196, 995, 1070
Bst2UI CCWGG 3 cut(s) 629, 1068, 1080
Bst4CI ACNGT 3 cut(s) 37, 84, 334
BstAUI TGTACA 1 cut(s) 1051
BstC8I GCNNGC 4 cut(s) 70, 89, 614, 1091
BstDEI CTNAG 1 cut(s) 163
BstF5I GGATG 4 cut(s) 118, 149, 766, 1046
BstFNI CGCG 1 cut(s) 306
BstKTI GATC 4 cut(s) 185, 199, 998, 1073
BstMBI GATC 4 cut(s) 182, 196, 995, 1070
BstMWI GCNNNNNNNGC 3 cut(s) 147, 156, 165
BstNI CCWGG 3 cut(s) 629, 1068, 1080
BstNSI RCATGY 4 cut(s) 616, 913, 1058, 1062
BstPAI GACNNNNGTC 1 cut(s) 563
BstSCI CCNGG 5 cut(s) 128, 578, 627, 1066, 1078
BstSFI CTRYAG 1 cut(s) 459
BstUI CGCG 1 cut(s) 306
BstV1I GCAGC 3 cut(s) 56, 919, 922
BstV2I GAAGAC 2 cut(s) 11, 559
BsuRI GGCC 2 cut(s) 627, 637
BtsCI GGATG 4 cut(s) 118, 149, 766, 1046
BtsIMutI CAGTG 4 cut(s) 89, 291, 467, 734
Cac8I GCNNGC 4 cut(s) 70, 89, 614, 1091
CseI GACGC 1 cut(s) 46
Csp6I GTAC 1 cut(s) 1052
CviAII CATG 7 cut(s) 613, 652, 739, 910, 926, 1055, 1059
CviQI GTAC 1 cut(s) 1052
DdeI CTNAG 1 cut(s) 163
DpnI GATC 4 cut(s) 184, 198, 997, 1072
DpnII GATC 4 cut(s) 182, 196, 995, 1070
EaeI YGGCCR 2 cut(s) 625, 635
Ecl136II GAGCTC 1 cut(s) 1030
Eco24I GRGCYC 1 cut(s) 1032
Eco53kI GAGCTC 1 cut(s) 1030
Eco57I CTGAAG 1 cut(s) 491
EcoICRI GAGCTC 1 cut(s) 1030
EcoRI GAATTC 1 cut(s) 297
EcoRII CCWGG 3 cut(s) 627, 1066, 1078
EcoT22I ATGCAT 1 cut(s) 1060
EcoT38I GRGCYC 1 cut(s) 1032
FaeI CATG 7 cut(s) 616, 655, 742, 913, 929, 1058, 1062
FalI AAGNNNNNCTT 2 cut(s) 117, 149
FatI CATG 7 cut(s) 612, 651, 738, 909, 925, 1054, 1058
FauNDI CATATG 1 cut(s) 382
Fnu4HI GCNGC 3 cut(s) 45, 933, 936
FokI GGATG 4 cut(s) 125, 156, 773, 1053
FriOI GRGCYC 1 cut(s) 1032
Fsp4HI GCNGC 3 cut(s) 45, 933, 936
FspBI CTAG 3 cut(s) 171, 641, 872
GluI GCNGC 3 cut(s) 45, 933, 936
GsuI CTGGAG 1 cut(s) 1062
HaeIII GGCC 2 cut(s) 627, 637
HapII CCGG 3 cut(s) 129, 579, 602
HgaI GACGC 1 cut(s) 46
Hin1II CATG 7 cut(s) 616, 655, 742, 913, 929, 1058, 1062
HindIII AAGCTT 3 cut(s) 66, 89, 1005
HinfI GANTC 4 cut(s) 7, 717, 800, 898
HpaII CCGG 3 cut(s) 129, 579, 602
HphI GGTGA 4 cut(s) 616, 798, 853, 1065
Hpy166II GTNNAC 1 cut(s) 684
Hpy188I TCNGA 3 cut(s) 508, 712, 903
Hpy188III TCNNGA 3 cut(s) 171, 323, 812
Hpy8I GTNNAC 1 cut(s) 684
Hpy99I CGWCG 1 cut(s) 62
HpyAV CCTTC 7 cut(s) 322, 466, 694, 707, 766, 770, 980
HpyCH4III ACNGT 3 cut(s) 37, 84, 334
HpyCH4IV ACGT 1 cut(s) 564
HpyCH4V TGCA 4 cut(s) 87, 269, 446, 1058
HpyF10VI GCNNNNNNNGC 3 cut(s) 147, 156, 165
HpyF3I CTNAG 1 cut(s) 163
HpySE526I ACGT 1 cut(s) 564
Hsp92II CATG 7 cut(s) 616, 655, 742, 913, 929, 1058, 1062
Kzo9I GATC 4 cut(s) 182, 196, 995, 1070
LmnI GCTCC 3 cut(s) 77, 550, 1027
Lsp1109I GCAGC 3 cut(s) 56, 919, 922
LweI GCATC 2 cut(s) 103, 360
MaeI CTAG 3 cut(s) 171, 641, 872
MaeII ACGT 1 cut(s) 564
MaeIII GTNAC 7 cut(s) 27, 307, 334, 514, 604, 647, 1043
MalI GATC 4 cut(s) 184, 198, 997, 1072
MboI GATC 4 cut(s) 182, 196, 995, 1070
MboII GAAGA 4 cut(s) 16, 338, 559, 598
MfeI CAATTG 1 cut(s) 270
MhlI GDGCHC 1 cut(s) 1032
MlsI TGGCCA 2 cut(s) 627, 637
MluCI AATT 6 cut(s) 200, 217, 270, 297, 510, 844
MluNI TGGCCA 2 cut(s) 627, 637
MmeI TCCRAC 1 cut(s) 99
MnlI CCTC 9 cut(s) 268, 312, 523, 663, 788, 819, 972, 986, 1086
Mox20I TGGCCA 2 cut(s) 627, 637
Mph1103I ATGCAT 1 cut(s) 1060
MroXI GAANNNNTTC 1 cut(s) 784
MscI TGGCCA 2 cut(s) 627, 637
MseI TTAA 4 cut(s) 390, 420, 867, 906
MslI CAYNNNNRTG 1 cut(s) 1059
Msp20I TGGCCA 2 cut(s) 627, 637
MspA1I CMGCKG 1 cut(s) 47
MspI CCGG 3 cut(s) 129, 579, 602
MspR9I CCNGG 5 cut(s) 130, 580, 629, 1068, 1080
MunI CAATTG 1 cut(s) 270
Mva1269I GAATGC 1 cut(s) 107
MvaI CCWGG 3 cut(s) 629, 1068, 1080
MvnI CGCG 1 cut(s) 306
MwoI GCNNNNNNNGC 3 cut(s) 147, 156, 165
NciI CCSGG 2 cut(s) 130, 580
NdeI CATATG 1 cut(s) 382
NdeII GATC 4 cut(s) 182, 196, 995, 1070
NlaIII CATG 7 cut(s) 616, 655, 742, 913, 929, 1058, 1062
NmuCI GTSAC 3 cut(s) 514, 604, 647
NsiI ATGCAT 1 cut(s) 1060
NspI RCATGY 4 cut(s) 616, 913, 1058, 1062
PaeI GCATGC 1 cut(s) 616
PctI GAATGC 1 cut(s) 107
PdmI GAANNNNTTC 1 cut(s) 784
PfeI GAWTC 3 cut(s) 717, 800, 898
PflMI CCANNNNNTGG 1 cut(s) 945
PkrI GCNGC 3 cut(s) 46, 934, 937
PshAI GACNNNNGTC 1 cut(s) 563
Psp124BI GAGCTC 1 cut(s) 1032
Psp6I CCWGG 3 cut(s) 627, 1066, 1078
PspGI CCWGG 3 cut(s) 627, 1066, 1078
RsaI GTAC 1 cut(s) 1053
RsaNI GTAC 1 cut(s) 1052
RseI CAYNNNNRTG 1 cut(s) 1059
SacI GAGCTC 1 cut(s) 1032
SaqAI TTAA 4 cut(s) 390, 420, 867, 906
SatI GCNGC 3 cut(s) 45, 933, 936
Sau3AI GATC 4 cut(s) 182, 196, 995, 1070
ScrFI CCNGG 5 cut(s) 130, 580, 629, 1068, 1080
SduI GDGCHC 1 cut(s) 1032
SfaNI GCATC 2 cut(s) 103, 360
SfcI CTRYAG 1 cut(s) 459
SmiMI CAYNNNNRTG 1 cut(s) 1059
SpeI ACTAGT 1 cut(s) 640
SphI GCATGC 1 cut(s) 616
Sse9I AATT 6 cut(s) 200, 217, 270, 297, 510, 844
SsiI CCGC 2 cut(s) 47, 141
SspMI CTAG 3 cut(s) 171, 641, 872
SstI GAGCTC 1 cut(s) 1032
StyD4I CCNGG 5 cut(s) 128, 578, 627, 1066, 1078
TaaI ACNGT 3 cut(s) 37, 84, 334
TaiI ACGT 1 cut(s) 567
TaqI TCGA 3 cut(s) 107, 361, 375
TasI AATT 6 cut(s) 200, 217, 270, 297, 510, 844
TatI WGTACW 1 cut(s) 1051
TfiI GAWTC 3 cut(s) 717, 800, 898
Tru1I TTAA 4 cut(s) 390, 420, 867, 906
Tru9I TTAA 4 cut(s) 390, 420, 867, 906
TscAI CASTG 4 cut(s) 89, 298, 474, 734
TseFI GTSAC 3 cut(s) 514, 604, 647
TseI GCWGC 3 cut(s) 44, 932, 935
Tsp45I GTSAC 3 cut(s) 514, 604, 647
TspDTI ATGAA 2 cut(s) 17, 785
TspGWI ACGGA 1 cut(s) 93
TspRI CASTG 4 cut(s) 89, 298, 474, 734
Van91I CCANNNNNTGG 1 cut(s) 945
XapI RAATTY 1 cut(s) 297
XbaI TCTAGA 1 cut(s) 170
XceI RCATGY 4 cut(s) 616, 913, 1058, 1062
XcmI CCANNNNNNNNNTGG 1 cut(s) 419
XmnI GAANNNNTTC 1 cut(s) 784
XspI CTAG 3 cut(s) 171, 641, 872
Zsp2I ATGCAT 1 cut(s) 1060
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.