Rmu_sc0002341.1_g000013

PI-PLC X domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002341.1
OG0001750
Physical Location & Seq
Forward (+)
56529 .. 66382
9854 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002341.1_g000013.1.cds

Sequence Viewer

Length: 1656 bp
atgggtgaacttactctaagagataacatagagatgattattaattctcttgtccaaatatggattttaacccgtgacttgaaccacgaagctgccgctgatcttcccaatctcggcttccgtctgcggactagaatcggaggcgttggactccgattcaaagatcctccgattcgttggaatccgtttgggagcctcggacgtagagtgggccagaatcgcaatcgggactgggcttgttggatcctccaaggaagggaccacataagcaacgacctcgtagatgggtcccgtgacatggttagtcccgcctacgacatgcatcgggtatccgacacccgagctacctcggtatggtggaggtcagccgggagcctccgattcgttggaatccgtctgggagcctcggatgtggagcgggccgaaatcggaatcgagtttgcaatcgggctgggcttgttggatcctccaaggaagggtccgtgctcgacggacggagattgtgaggcggggctttactgtttcggttgcccttcggagttttctggctccagatgtgtgagatcaaccaccaccaaccaattccagcttttgaataattctctaccattcaacaaatatgcatttttgacaacccacaatgcttttgctatcgaaggagagccatctcataccggagtccctcgtgttaccttcacgaatcaagaagacactgtcactcaacaacttaacaatggagttagagccctaatgcttgatacctatgattttcaaggagatgtctggttgtgccattccttcaaaggaaaatgccatgactacactgcatttgagccagctatagatactttgaatgaaatccaagcatttttatcagcaaacccaggagaaattgtgacattgatattagaggactatgttgaagctccaaacggattgacaaatgttttcaaagctgccggattgatgaaatactggtttccggtatcaaacatgcccaaaagtggtcaggattggccgctggttagcgatatggtcactaagaaccaaaggctacttgtattcacttcaaaacaagagaaggaacaatccgaagggattgcataccagtggaactacatggttaaaaaccagtatggaaatgatggaatgaaagcgggaagctgttcaaacagagctgaatcgtctcctctaaatgacaagactaaatcattggtgttggttaactattttgggtcagttcccattaagcaactctcatgtcaattcaattctgaggatttggttagcatgcttaacacttgctatggtgctgctggaaaccgatgggcaaattttgttgcagttgatttttacaagaggagtggaggagggggatcatttcaagctacagacactctcaatggagaactcatatgtggatgtaacgatgtccatgcatgtgtgaggcatcagaacctaaaggccgagagggttccttcctcggcctacaatcatttgaaagaagtcagatcacgcaccattcaaatcagaaacctcgctcggccgttcggccgcgagttggcacgcccgcgcaatttcagaaggacaattcaaccacaagtcgctcggctcgcatttcagccgagacgatttttaggcaaacatagactttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

551

Amino Acids

61.85

Weight (kDa)

8.76

Isoelectric Point (pI)

44.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 418
AccII CGCG 2 cut(s) 1557, 1573
AciI CCGC 9 cut(s) 96, 127, 309, 418, 509, 1019, 1157, 1555, 1571
AclWI GGATC 6 cut(s) 158, 238, 251, 458, 471, 1384
AcoI YGGCCR 3 cut(s) 1016, 1544, 1552
AcsI RAATTY 1 cut(s) 1333
AfiI CCNNNNNNNGG 7 cut(s) 113, 256, 298, 354, 476, 1004, 1561
AjnI CCWGG 1 cut(s) 883
AjuI GAANNNNNNNTTGG 2 cut(s) 101, 133
AluBI AGCT 9 cut(s) 92, 344, 589, 839, 926, 956, 1164, 1178, 1388
AluI AGCT 9 cut(s) 92, 344, 589, 839, 926, 956, 1164, 1178, 1388
Alw21I GWGCWC 1 cut(s) 488
Alw26I GTCTC 2 cut(s) 1191, 1621
AlwI GGATC 6 cut(s) 158, 238, 251, 458, 471, 1384
Ama87I CYCGRG 1 cut(s) 339
AoxI GGCC 7 cut(s) 211, 420, 1016, 1464, 1485, 1544, 1552
ApeKI GCWGC 3 cut(s) 92, 956, 1313
ApoI RAATTY 1 cut(s) 1333
AseI ATTAAT 1 cut(s) 42
Asp700I GAANNNNTTC 1 cut(s) 1165
AspLEI GCGC 1 cut(s) 1575
AspS9I GGNCC 5 cut(s) 211, 259, 288, 420, 479
AsuC2I CCSGG 1 cut(s) 370
AsuHPI GGTGA 1 cut(s) 17
AvaI CYCGRG 1 cut(s) 339
AvaII GGWCC 3 cut(s) 259, 288, 479
BamHI GGATCC 2 cut(s) 243, 463
BanII GRGCYC 1 cut(s) 748
BauI CACGAG 1 cut(s) 684
BbsI GAAGAC 1 cut(s) 714
Bbv12I GWGCWC 1 cut(s) 488
BbvI GCAGC 3 cut(s) 79, 943, 1300
BccI CCATC 4 cut(s) 278, 673, 1139, 1320
BceAI ACGGC 1 cut(s) 1531
BcgI CGANNNNNNTGC 6 cut(s) 259, 293, 1082, 1116, 1418, 1452
BciT130I CCWGG 1 cut(s) 885
BciVI GTATCC 1 cut(s) 340
BcnI CCSGG 1 cut(s) 370
BcoDI GTCTC 2 cut(s) 1191, 1621
BfaI CTAG 1 cut(s) 132
BfmI CTRYAG 2 cut(s) 840, 1389
BfuI GTATCC 1 cut(s) 340
BisI GCNGC 6 cut(s) 93, 96, 957, 1019, 1314, 1555
BlsI GCNGC 6 cut(s) 94, 97, 958, 1020, 1315, 1556
Bme1390I CCNGG 2 cut(s) 370, 885
Bme18I GGWCC 3 cut(s) 259, 288, 479
BmeT110I CYCGRG 1 cut(s) 339
BmgT120I GGNCC 5 cut(s) 211, 259, 288, 420, 479
BmrFI CCNGG 2 cut(s) 370, 885
BmrI ACTGGG 1 cut(s) 241
BmsI GCATC 2 cut(s) 331, 1459
BmuI ACTGGG 1 cut(s) 241
BpiI GAAGAC 1 cut(s) 714
BpmI CTGGAG 1 cut(s) 535
BpuMI CCSGG 1 cut(s) 370
BsaJI CCNNGG 7 cut(s) 196, 250, 348, 405, 470, 883, 1482
BsaWI WCCGGW 2 cut(s) 674, 982
BsaXI ACNNNNNCTCC 2 cut(s) 879, 909
Bsc4I CCNNNNNNNGG 7 cut(s) 113, 256, 298, 354, 476, 1004, 1561
Bse1I ACTGG 4 cut(s) 236, 980, 1108, 1132
BseBI CCWGG 1 cut(s) 885
BseDI CCNNGG 7 cut(s) 196, 250, 348, 405, 470, 883, 1482
BseGI GGATG 2 cut(s) 415, 1427
BseLI CCNNNNNNNGG 7 cut(s) 113, 256, 298, 354, 476, 1004, 1561
BseMII CTCAG 1 cut(s) 1266
BseNI ACTGG 4 cut(s) 236, 980, 1108, 1132
BseRI GAGGAG 3 cut(s) 1179, 1375, 1383
BseX3I CGGCCG 2 cut(s) 1544, 1552
BseXI GCAGC 3 cut(s) 79, 943, 1300
BseYI CCCAGC 1 cut(s) 451
Bsh1236I CGCG 2 cut(s) 1557, 1573
Bsh1285I CGRYCG 2 cut(s) 1547, 1555
BshFI GGCC 7 cut(s) 213, 422, 1018, 1466, 1487, 1546, 1554
BsiEI CGRYCG 2 cut(s) 1547, 1555
BsiHKAI GWGCWC 1 cut(s) 488
BsiHKCI CYCGRG 1 cut(s) 339
BsiSI CCGG 4 cut(s) 369, 675, 960, 983
BslFI GGGAC 5 cut(s) 242, 272, 274, 291, 665
BslI CCNNNNNNNGG 7 cut(s) 113, 256, 298, 354, 476, 1004, 1561
BsmAI GTCTC 2 cut(s) 1191, 1621
BsmBI CGTCTC 2 cut(s) 1191, 1621
BsmFI GGGAC 5 cut(s) 242, 272, 274, 291, 665
BsnI GGCC 7 cut(s) 213, 422, 1018, 1466, 1487, 1546, 1554
BsoBI CYCGRG 1 cut(s) 339
Bsp1286I GDGCHC 2 cut(s) 488, 748
Bsp143I GATC 7 cut(s) 100, 163, 243, 463, 563, 1376, 1511
BspACI CCGC 9 cut(s) 96, 127, 309, 418, 509, 1019, 1157, 1555, 1571
BspANI GGCC 7 cut(s) 213, 422, 1018, 1466, 1487, 1546, 1554
BspCNI CTCAG 1 cut(s) 1267
BspFNI CGCG 2 cut(s) 1557, 1573
BspPI GGATC 6 cut(s) 158, 238, 251, 458, 471, 1384
BsrBI CCGCTC 1 cut(s) 418
BsrI ACTGG 4 cut(s) 236, 980, 1108, 1132
BssECI CCNNGG 7 cut(s) 196, 250, 348, 405, 470, 883, 1482
BssMI GATC 7 cut(s) 100, 163, 243, 463, 563, 1376, 1511
BssSI CACGAG 1 cut(s) 684
BssT1I CCWWGG 2 cut(s) 250, 470
Bst2BI CACGAG 1 cut(s) 684
Bst2UI CCWGG 1 cut(s) 885
Bst4CI ACNGT 2 cut(s) 521, 715
BstC8I GCNNGC 6 cut(s) 420, 837, 1292, 1567, 1571, 1614
BstDEI CTNAG 3 cut(s) 17, 1041, 1275
BstF5I GGATG 2 cut(s) 415, 1427
BstFNI CGCG 2 cut(s) 1557, 1573
BstHHI GCGC 1 cut(s) 1575
BstKTI GATC 7 cut(s) 103, 166, 246, 466, 566, 1379, 1514
BstMAI GTCTC 2 cut(s) 1191, 1621
BstMBI GATC 7 cut(s) 100, 163, 243, 463, 563, 1376, 1511
BstMCI CGRYCG 2 cut(s) 1547, 1555
BstMWI GCNNNNNNNGC 2 cut(s) 219, 1613
BstNI CCWGG 1 cut(s) 885
BstNSI RCATGY 4 cut(s) 322, 997, 1294, 1443
BstSCI CCNGG 2 cut(s) 368, 883
BstSFI CTRYAG 2 cut(s) 840, 1389
BstUI CGCG 2 cut(s) 1557, 1573
BstV1I GCAGC 3 cut(s) 79, 943, 1300
BstV2I GAAGAC 1 cut(s) 714
BstX2I RGATCY 3 cut(s) 163, 243, 463
BstYI RGATCY 3 cut(s) 163, 243, 463
BstZI CGGCCG 2 cut(s) 1544, 1552
BsuI GTATCC 1 cut(s) 340
BsuRI GGCC 7 cut(s) 213, 422, 1018, 1466, 1487, 1546, 1554
BtsCI GGATG 2 cut(s) 415, 1427
BtsI GCAGTG 1 cut(s) 822
BtsIMutI CAGTG 3 cut(s) 711, 822, 1115
Cac8I GCNNGC 6 cut(s) 420, 837, 1292, 1567, 1571, 1614
CfoI GCGC 1 cut(s) 1575
Cfr13I GGNCC 5 cut(s) 211, 259, 288, 420, 479
CspCI CAANNNNNGTGG 2 cut(s) 1345, 1380
CviAII CATG 9 cut(s) 298, 319, 815, 994, 1120, 1260, 1291, 1436, 1440
DdeI CTNAG 3 cut(s) 17, 1041, 1275
DpnI GATC 7 cut(s) 102, 165, 245, 465, 565, 1378, 1513
DpnII GATC 7 cut(s) 100, 163, 243, 463, 563, 1376, 1511
EaeI YGGCCR 3 cut(s) 1016, 1544, 1552
EagI CGGCCG 2 cut(s) 1544, 1552
EclXI CGGCCG 2 cut(s) 1544, 1552
Eco130I CCWWGG 2 cut(s) 250, 470
Eco24I GRGCYC 1 cut(s) 748
Eco47I GGWCC 3 cut(s) 259, 288, 479
Eco52I CGGCCG 2 cut(s) 1544, 1552
Eco88I CYCGRG 1 cut(s) 339
EcoO109I RGGNCCY 1 cut(s) 288
EcoRII CCWGG 1 cut(s) 883
EcoT14I CCWWGG 2 cut(s) 250, 470
EcoT22I ATGCAT 3 cut(s) 324, 625, 1441
EcoT38I GRGCYC 1 cut(s) 748
ErhI CCWWGG 2 cut(s) 250, 470
Esp3I CGTCTC 2 cut(s) 1191, 1621
FaeI CATG 9 cut(s) 301, 322, 818, 997, 1123, 1263, 1294, 1439, 1443
FaqI GGGAC 5 cut(s) 242, 272, 274, 291, 665
FatI CATG 9 cut(s) 297, 318, 814, 993, 1119, 1259, 1290, 1435, 1439
FauI CCCGC 5 cut(s) 316, 411, 502, 1150, 1578
FauNDI CATATG 1 cut(s) 1415
Fnu4HI GCNGC 6 cut(s) 93, 96, 957, 1019, 1314, 1555
FokI GGATG 2 cut(s) 422, 1434
FriOI GRGCYC 1 cut(s) 748
Fsp4HI GCNGC 6 cut(s) 93, 96, 957, 1019, 1314, 1555
FspBI CTAG 1 cut(s) 132
GlaI GCGC 1 cut(s) 1574
GluI GCNGC 6 cut(s) 93, 96, 957, 1019, 1314, 1555
GsaI CCCAGC 1 cut(s) 455
GsuI CTGGAG 1 cut(s) 535
HaeIII GGCC 7 cut(s) 213, 422, 1018, 1466, 1487, 1546, 1554
HapII CCGG 4 cut(s) 369, 675, 960, 983
HhaI GCGC 1 cut(s) 1575
Hin1II CATG 9 cut(s) 301, 322, 818, 997, 1123, 1263, 1294, 1439, 1443
Hin6I GCGC 1 cut(s) 1573
HinP1I GCGC 1 cut(s) 1573
HincII GTYRAC 1 cut(s) 1225
HindII GTYRAC 1 cut(s) 1225
HpaI GTTAAC 1 cut(s) 1225
HpaII CCGG 4 cut(s) 369, 675, 960, 983
HphI GGTGA 1 cut(s) 17
Hpy166II GTNNAC 2 cut(s) 8, 1225
Hpy188III TCNNGA 5 cut(s) 227, 552, 697, 704, 1010
Hpy8I GTNNAC 2 cut(s) 8, 1225
Hpy99I CGWCG 1 cut(s) 493
HpyCH4III ACNGT 2 cut(s) 521, 715
HpyCH4IV ACGT 1 cut(s) 202
HpyCH4V TGCA 7 cut(s) 322, 443, 623, 827, 1103, 1343, 1439
HpyF10VI GCNNNNNNNGC 2 cut(s) 219, 1613
HpyF3I CTNAG 3 cut(s) 17, 1041, 1275
HpySE526I ACGT 1 cut(s) 202
Hsp92II CATG 9 cut(s) 301, 322, 818, 997, 1123, 1263, 1294, 1439, 1443
HspAI GCGC 1 cut(s) 1573
KflI GGGWCCC 1 cut(s) 288
KspAI GTTAAC 1 cut(s) 1225
Kzo9I GATC 7 cut(s) 100, 163, 243, 463, 563, 1376, 1511
LmnI GCTCC 6 cut(s) 192, 372, 401, 415, 554, 931
Lsp1109I GCAGC 3 cut(s) 79, 943, 1300
LweI GCATC 2 cut(s) 331, 1459
MaeI CTAG 1 cut(s) 132
MaeII ACGT 1 cut(s) 202
MaeIII GTNAC 7 cut(s) 74, 293, 688, 715, 895, 1036, 1424
MalI GATC 7 cut(s) 102, 165, 245, 465, 565, 1378, 1513
MbiI CCGCTC 1 cut(s) 418
MboI GATC 7 cut(s) 100, 163, 243, 463, 563, 1376, 1511
MboII GAAGA 2 cut(s) 95, 719
MflI RGATCY 3 cut(s) 163, 243, 463
MhlI GDGCHC 2 cut(s) 488, 748
MluCI AATT 9 cut(s) 43, 581, 598, 891, 1265, 1270, 1333, 1576, 1590
MlyI GAGTC 2 cut(s) 144, 687
MmeI TCCRAC 6 cut(s) 127, 158, 221, 357, 367, 441
Mph1103I ATGCAT 3 cut(s) 324, 625, 1441
MroXI GAANNNNTTC 1 cut(s) 1165
MseI TTAA 7 cut(s) 42, 68, 729, 1125, 1224, 1248, 1296
MslI CAYNNNNRTG 3 cut(s) 32, 1108, 1440
MspA1I CMGCKG 2 cut(s) 98, 1021
MspI CCGG 4 cut(s) 369, 675, 960, 983
MspR9I CCNGG 2 cut(s) 370, 885
MvaI CCWGG 1 cut(s) 885
MvnI CGCG 2 cut(s) 1557, 1573
MwoI GCNNNNNNNGC 2 cut(s) 219, 1613
NciI CCSGG 1 cut(s) 370
NdeI CATATG 1 cut(s) 1415
NdeII GATC 7 cut(s) 100, 163, 243, 463, 563, 1376, 1511
NlaIII CATG 9 cut(s) 301, 322, 818, 997, 1123, 1263, 1294, 1439, 1443
NmeAIII GCCGAG 6 cut(s) 93, 1463, 1492, 1522, 1588, 1650
NmuCI GTSAC 5 cut(s) 74, 293, 715, 895, 1036
NsiI ATGCAT 3 cut(s) 324, 625, 1441
NspI RCATGY 4 cut(s) 322, 997, 1294, 1443
PaeI GCATGC 1 cut(s) 1294
PdmI GAANNNNTTC 1 cut(s) 1165
PflFI GACNNNGTC 1 cut(s) 713
PkrI GCNGC 6 cut(s) 94, 97, 958, 1020, 1315, 1556
PleI GAGTC 2 cut(s) 144, 686
PpsI GAGTC 2 cut(s) 144, 686
PpuMI RGGWCCY 1 cut(s) 288
PshBI ATTAAT 1 cut(s) 42
Psp5II RGGWCCY 1 cut(s) 288
Psp6I CCWGG 1 cut(s) 883
PspFI CCCAGC 1 cut(s) 451
PspGI CCWGG 1 cut(s) 883
PspPI GGNCC 5 cut(s) 211, 259, 288, 420, 479
PspPPI RGGWCCY 1 cut(s) 288
PsuI RGATCY 3 cut(s) 163, 243, 463
PsyI GACNNNGTC 1 cut(s) 713
RseI CAYNNNNRTG 3 cut(s) 32, 1108, 1440
SaqAI TTAA 7 cut(s) 42, 68, 729, 1125, 1224, 1248, 1296
SatI GCNGC 6 cut(s) 93, 96, 957, 1019, 1314, 1555
Sau3AI GATC 7 cut(s) 100, 163, 243, 463, 563, 1376, 1511
Sau96I GGNCC 5 cut(s) 211, 259, 288, 420, 479
SchI GAGTC 2 cut(s) 144, 687
ScrFI CCNGG 2 cut(s) 370, 885
SduI GDGCHC 2 cut(s) 488, 748
SfaNI GCATC 2 cut(s) 331, 1459
SfcI CTRYAG 2 cut(s) 840, 1389
SinI GGWCC 3 cut(s) 259, 288, 479
SmiMI CAYNNNNRTG 3 cut(s) 32, 1108, 1440
SphI GCATGC 1 cut(s) 1294
Sse9I AATT 9 cut(s) 43, 581, 598, 891, 1265, 1270, 1333, 1576, 1590
SsiI CCGC 9 cut(s) 96, 127, 309, 418, 509, 1019, 1157, 1555, 1571
SspMI CTAG 1 cut(s) 132
StyD4I CCNGG 2 cut(s) 368, 883
StyI CCWWGG 2 cut(s) 250, 470
TaaI ACNGT 2 cut(s) 521, 715
TaiI ACGT 1 cut(s) 205
TaqI TCGA 3 cut(s) 435, 488, 654
TasI AATT 9 cut(s) 43, 581, 598, 891, 1265, 1270, 1333, 1576, 1590
TauI GCSGC 3 cut(s) 98, 1021, 1557
Tru1I TTAA 7 cut(s) 42, 68, 729, 1125, 1224, 1248, 1296
Tru9I TTAA 7 cut(s) 42, 68, 729, 1125, 1224, 1248, 1296
TscAI CASTG 3 cut(s) 718, 829, 1115
TseFI GTSAC 5 cut(s) 74, 293, 715, 895, 1036
TseI GCWGC 3 cut(s) 92, 956, 1313
Tsp45I GTSAC 5 cut(s) 74, 293, 715, 895, 1036
TspDTI ATGAA 3 cut(s) 870, 983, 1166
TspGWI ACGGA 7 cut(s) 110, 174, 383, 471, 506, 510, 948
TspRI CASTG 3 cut(s) 718, 829, 1115
Tth111I GACNNNGTC 1 cut(s) 713
VpaK11BI GGWCC 3 cut(s) 259, 288, 479
VspI ATTAAT 1 cut(s) 42
XapI RAATTY 1 cut(s) 1333
XceI RCATGY 4 cut(s) 322, 997, 1294, 1443
XmnI GAANNNNTTC 1 cut(s) 1165
XspI CTAG 1 cut(s) 132
Zsp2I ATGCAT 3 cut(s) 324, 625, 1441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.