RchiOBHm_Chr1g0321041

PI-PLC X domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001750
Physical Location & Seq
Reverse (-)
8499489 .. 8500761
1273 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55112

Sequence Viewer

Length: 990 bp
ATGAAGACATGGAATGTCACCTCACCATGTGCTACACAAACTTGGTACTTCTTTTTTTCCAGTAGCATTGCTAATCTCAAATGCGATTTAAGTAAAATTTGCTTATTTGTGGTTCTACAGAATAATTCTCTACCATTCAACAAATATGCATTTTTGACAACCCACAATGCTTTTGCTATCGAAGGAGAGCCATCTCATACCGGAGTCCCTCGTGTTACCTTCACGAATCAAGAAGACACTGTCACTCAACAACTTAACAATGGAGTTAGAGCCCTAATGCTTGATACCTATGATTTTAAAGGAGATGTCTGGTTGTGCCATTCCTTCAAAGGAAAATGCCATGACTACACTGCATTTGAGCCAGCTATAGATACATTGAATGATATCCAAGCATTTTTATCAGCAAACCTAGGAGAAATTGTGACATTGATATTAGAGGACTATGTTGAAGCTCCAAACGGATTGACAAATGTTTTCAGAGCTGCCGGATTGATGAAATACTGGTTTCCGACATCAAACATGCCCAAAAGTGGTCAGGATTGGCCGCTGGTTAGCGATATGGTCACTAAGAACCAAAGGCTACTTGTATTCACTTCAAAACAAGAGAAGGAACAATCCGAAGGGATTGCATACCAGTGGAACTACATGGTTGAAAACCAGTATGGAAATGATGGAATGAAAGCGGGAAGCTGTTCAAACAGAGCTGAATCGTCGCCTCTAAATGACAAGACTAAATCATTGGTGTTGGTTAACTATTTTGGGTCAGTTCCCATTAAGCAGCTCTCATGTCAATTCAATTCTGAGGATTTGGTTAGCATGCTTAACACTTGCTATGGTGCTGCTGGAAACCGATGGGAGAATTTTGTTGCGGTTGATTTTTACAAGAGGAGTGGAGGAGGGGGATCATTTCAAGCTATAGACACTCTCAATGGAGAACTCATATGTGGATGTAACGATGTCCATGCATGTGTGGTAAGCTATGAAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

329

Amino Acids

36.76

Weight (kDa)

5.07

Isoelectric Point (pI)

36.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC_cat PF26178 40 - 323 7.5e-148 PI-PLC-like catalytic domain
PI-PLC_X PF26146 71 - 262 1.9e-23 PI-PLC X
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 545, 683, 869
AclWI GGATC 1 cut(s) 910
AcoI YGGCCR 1 cut(s) 542
AcsI RAATTY 2 cut(s) 96, 859
AdeI CACNNNGTG 1 cut(s) 29
AfaI GTAC 1 cut(s) 47
AfiI CCNNNNNNNGG 1 cut(s) 530
AgsI TTSAA 9 cut(s) 139, 328, 379, 449, 597, 653, 696, 796, 911
AluBI AGCT 8 cut(s) 365, 452, 482, 690, 704, 781, 914, 978
AluI AGCT 8 cut(s) 365, 452, 482, 690, 704, 781, 914, 978
AlwI GGATC 1 cut(s) 910
AoxI GGCC 1 cut(s) 542
ApeKI GCWGC 3 cut(s) 482, 778, 839
ApoI RAATTY 2 cut(s) 96, 859
Asp700I GAANNNNTTC 1 cut(s) 691
AspA2I CCTAGG 1 cut(s) 409
AsuHPI GGTGA 2 cut(s) 10, 15
AvrII CCTAGG 1 cut(s) 409
BanII GRGCYC 1 cut(s) 274
BauI CACGAG 1 cut(s) 210
BbsI GAAGAC 2 cut(s) 11, 240
BbvI GCAGC 3 cut(s) 469, 790, 826
BccI CCATC 3 cut(s) 199, 665, 846
BcgI CGANNNNNNTGC 4 cut(s) 608, 642, 944, 978
BfaI CTAG 1 cut(s) 410
BfmI CTRYAG 3 cut(s) 116, 366, 915
BisI GCNGC 4 cut(s) 483, 545, 779, 840
BlnI CCTAGG 1 cut(s) 409
BlsI GCNGC 4 cut(s) 484, 546, 780, 841
BpiI GAAGAC 2 cut(s) 11, 240
BsaJI CCNNGG 1 cut(s) 409
BsaWI WCCGGW 1 cut(s) 200
BsaXI ACNNNNNCTCC 2 cut(s) 405, 435
Bsc4I CCNNNNNNNGG 1 cut(s) 530
Bse1I ACTGG 4 cut(s) 60, 506, 634, 658
Bse3DI GCAATG 1 cut(s) 66
BseDI CCNNGG 1 cut(s) 409
BseGI GGATG 1 cut(s) 953
BseLI CCNNNNNNNGG 1 cut(s) 530
BseMI GCAATG 1 cut(s) 66
BseMII CTCAG 1 cut(s) 792
BseNI ACTGG 4 cut(s) 60, 506, 634, 658
BseRI GAGGAG 2 cut(s) 901, 909
BseXI GCAGC 3 cut(s) 469, 790, 826
BshFI GGCC 1 cut(s) 544
BsiSI CCGG 2 cut(s) 201, 486
BslFI GGGAC 1 cut(s) 191
BslI CCNNNNNNNGG 1 cut(s) 530
BsmFI GGGAC 1 cut(s) 191
BsnI GGCC 1 cut(s) 544
Bsp1286I GDGCHC 1 cut(s) 274
Bsp143I GATC 1 cut(s) 902
BspACI CCGC 3 cut(s) 545, 683, 869
BspANI GGCC 1 cut(s) 544
BspCNI CTCAG 1 cut(s) 793
BspPI GGATC 1 cut(s) 910
BsrDI GCAATG 1 cut(s) 66
BsrI ACTGG 4 cut(s) 60, 506, 634, 658
BssECI CCNNGG 1 cut(s) 409
BssMI GATC 1 cut(s) 902
BssSI CACGAG 1 cut(s) 210
BssT1I CCWWGG 1 cut(s) 409
Bst2BI CACGAG 1 cut(s) 210
Bst4CI ACNGT 1 cut(s) 241
BstC8I GCNNGC 2 cut(s) 363, 818
BstDEI CTNAG 2 cut(s) 567, 801
BstF5I GGATG 1 cut(s) 953
BstKTI GATC 1 cut(s) 905
BstMBI GATC 1 cut(s) 902
BstNSI RCATGY 3 cut(s) 523, 820, 969
BstSFI CTRYAG 3 cut(s) 116, 366, 915
BstV1I GCAGC 3 cut(s) 469, 790, 826
BstV2I GAAGAC 2 cut(s) 11, 240
BsuRI GGCC 1 cut(s) 544
BtsCI GGATG 1 cut(s) 953
BtsI GCAGTG 1 cut(s) 348
BtsIMutI CAGTG 3 cut(s) 237, 348, 641
Cac8I GCNNGC 2 cut(s) 363, 818
Csp6I GTAC 1 cut(s) 46
CspCI CAANNNNNGTGG 2 cut(s) 871, 906
CviAII CATG 9 cut(s) 9, 27, 341, 520, 646, 786, 817, 962, 966
CviQI GTAC 1 cut(s) 46
DdeI CTNAG 2 cut(s) 567, 801
DpnI GATC 1 cut(s) 904
DpnII GATC 1 cut(s) 902
DraI TTTAAA 1 cut(s) 298
DraIII CACNNNGTG 1 cut(s) 29
EaeI YGGCCR 1 cut(s) 542
Eco130I CCWWGG 1 cut(s) 409
Eco24I GRGCYC 1 cut(s) 274
Eco32I GATATC 1 cut(s) 385
EcoRV GATATC 1 cut(s) 385
EcoT14I CCWWGG 1 cut(s) 409
EcoT22I ATGCAT 2 cut(s) 151, 967
EcoT38I GRGCYC 1 cut(s) 274
ErhI CCWWGG 1 cut(s) 409
FaeI CATG 9 cut(s) 12, 30, 344, 523, 649, 789, 820, 965, 969
FaqI GGGAC 1 cut(s) 191
FatI CATG 9 cut(s) 8, 26, 340, 519, 645, 785, 816, 961, 965
FauI CCCGC 1 cut(s) 676
FauNDI CATATG 1 cut(s) 941
Fnu4HI GCNGC 4 cut(s) 483, 545, 779, 840
FokI GGATG 1 cut(s) 960
FriOI GRGCYC 1 cut(s) 274
Fsp4HI GCNGC 4 cut(s) 483, 545, 779, 840
FspBI CTAG 1 cut(s) 410
GluI GCNGC 4 cut(s) 483, 545, 779, 840
HaeIII GGCC 1 cut(s) 544
HapII CCGG 2 cut(s) 201, 486
Hin1II CATG 9 cut(s) 12, 30, 344, 523, 649, 789, 820, 965, 969
HincII GTYRAC 1 cut(s) 751
HindII GTYRAC 1 cut(s) 751
HinfI GANTC 3 cut(s) 204, 226, 707
HpaI GTTAAC 1 cut(s) 751
HpaII CCGG 2 cut(s) 201, 486
HphI GGTGA 2 cut(s) 10, 15
Hpy166II GTNNAC 1 cut(s) 751
Hpy188I TCNGA 4 cut(s) 479, 510, 619, 802
Hpy188III TCNNGA 3 cut(s) 223, 230, 536
Hpy8I GTNNAC 1 cut(s) 751
Hpy99I CGWCG 1 cut(s) 715
HpyAV CCTTC 5 cut(s) 176, 229, 334, 601, 614
HpyCH4III ACNGT 1 cut(s) 241
HpyCH4V TGCA 4 cut(s) 149, 353, 629, 965
HpyF3I CTNAG 2 cut(s) 567, 801
Hsp92II CATG 9 cut(s) 12, 30, 344, 523, 649, 789, 820, 965, 969
KspAI GTTAAC 1 cut(s) 751
Kzo9I GATC 1 cut(s) 902
LmnI GCTCC 1 cut(s) 457
Lsp1109I GCAGC 3 cut(s) 469, 790, 826
MaeI CTAG 1 cut(s) 410
MaeIII GTNAC 6 cut(s) 16, 214, 241, 421, 562, 950
MalI GATC 1 cut(s) 904
MboI GATC 1 cut(s) 902
MboII GAAGA 2 cut(s) 16, 245
MhlI GDGCHC 1 cut(s) 274
MluCI AATT 6 cut(s) 96, 124, 417, 791, 796, 859
MlyI GAGTC 1 cut(s) 213
MmeI TCCRAC 1 cut(s) 533
MnlI CCTC 8 cut(s) 31, 219, 430, 726, 796, 879, 887, 890
Mph1103I ATGCAT 2 cut(s) 151, 967
MroXI GAANNNNTTC 1 cut(s) 691
MseI TTAA 6 cut(s) 89, 255, 297, 750, 774, 822
MslI CAYNNNNRTG 2 cut(s) 634, 966
MspA1I CMGCKG 1 cut(s) 547
MspI CCGG 2 cut(s) 201, 486
NdeI CATATG 1 cut(s) 941
NdeII GATC 1 cut(s) 902
NlaIII CATG 9 cut(s) 12, 30, 344, 523, 649, 789, 820, 965, 969
NmuCI GTSAC 4 cut(s) 16, 241, 421, 562
NsiI ATGCAT 2 cut(s) 151, 967
NspI RCATGY 3 cut(s) 523, 820, 969
PaeI GCATGC 1 cut(s) 820
PdmI GAANNNNTTC 1 cut(s) 691
PfeI GAWTC 2 cut(s) 226, 707
PflFI GACNNNGTC 1 cut(s) 239
PkrI GCNGC 4 cut(s) 484, 546, 780, 841
PleI GAGTC 1 cut(s) 212
PpsI GAGTC 1 cut(s) 212
PsyI GACNNNGTC 1 cut(s) 239
RsaI GTAC 1 cut(s) 47
RsaNI GTAC 1 cut(s) 46
RseI CAYNNNNRTG 2 cut(s) 634, 966
SaqAI TTAA 6 cut(s) 89, 255, 297, 750, 774, 822
SatI GCNGC 4 cut(s) 483, 545, 779, 840
Sau3AI GATC 1 cut(s) 902
SchI GAGTC 1 cut(s) 213
SduI GDGCHC 1 cut(s) 274
SfcI CTRYAG 3 cut(s) 116, 366, 915
SmiMI CAYNNNNRTG 2 cut(s) 634, 966
SphI GCATGC 1 cut(s) 820
Sse9I AATT 6 cut(s) 96, 124, 417, 791, 796, 859
SsiI CCGC 3 cut(s) 545, 683, 869
SspMI CTAG 1 cut(s) 410
StyI CCWWGG 1 cut(s) 409
TaaI ACNGT 1 cut(s) 241
TaqI TCGA 1 cut(s) 180
TasI AATT 6 cut(s) 96, 124, 417, 791, 796, 859
TauI GCSGC 1 cut(s) 547
TfiI GAWTC 2 cut(s) 226, 707
Tru1I TTAA 6 cut(s) 89, 255, 297, 750, 774, 822
Tru9I TTAA 6 cut(s) 89, 255, 297, 750, 774, 822
TscAI CASTG 3 cut(s) 244, 355, 641
TseFI GTSAC 4 cut(s) 16, 241, 421, 562
TseI GCWGC 3 cut(s) 482, 778, 839
Tsp45I GTSAC 4 cut(s) 16, 241, 421, 562
TspDTI ATGAA 3 cut(s) 17, 509, 692
TspGWI ACGGA 1 cut(s) 474
TspRI CASTG 3 cut(s) 244, 355, 641
Tth111I GACNNNGTC 1 cut(s) 239
XapI RAATTY 2 cut(s) 96, 859
XceI RCATGY 3 cut(s) 523, 820, 969
XmaJI CCTAGG 1 cut(s) 409
XmnI GAANNNNTTC 1 cut(s) 691
XspI CTAG 1 cut(s) 410
Zsp2I ATGCAT 2 cut(s) 151, 967
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.