RchiOBHm_Chr1g0320941

PI-PLC X domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001750
Physical Location & Seq
Reverse (-)
8442205 .. 8445342
3138 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55103

Sequence Viewer

Length: 1230 bp
ATGAAGACGACTCGGCACAGCTTCCTTCTGGTTCTAGTTCTGGTTATGGCTCTTGCTGTGTTTTCATGTGTCGTCATAGCTTGCTCCGATGGACAGTGCAAGCTCTTAGATCAGTGCTCGACGGACGGAGATTGTGAGGCGGGGCTTTACTGTTTCGGTTGCCCCTCGGAGTTTTCTGGCTCCAGATGTGTGAGATCAACCACCACCAACCAATTCCAGCTTTTGGTACCTGGTTTCAATTGGTCCCAGACATGGAATGTCACCTCATCATTTGCTACACAAACTTGGTACTTTTTTTCCAGTAGCATTGCTAATCTCAAATGCGATTTAAGTAAGATTTGCCTATTTGTGGTTCTACAGAATAATTCTCTACCATTCAACAAATATGCATTTTTGACAACCCACAATGCTTTTGCTATCGAAGGAGAGCCATCTCATACCGGAGTCCCTCGTGTTACCTTCACGAATCAAGAAGACATTGTCACTCAACAACTTAACAATGGAGTTAGAGCCCTAATGCTTGATACCTATGATTTTCAAGGAGATGTCTGGTTGTGCCATTCCTTCAAAGGAAAATGCCATGACTACACTGCATTTGAGCCAGCTATAGATACTTTGAATGAAATCCAAGCATTTTTATCAGCAAACCCAGGAGAAATTGTGACATTGATATTAGAGGACTATGTTGAAGCTCCAAACGGATTGACAAATGTTTTCAAAGCTGCCGGATTGATGAAATACTGGTTTCCGATATCAAACATGCCCAAAAGTGGTCAGGATTGGCCGCTGGTTAGCGATATGGTCACTAAGAACCAAAGGCTACTTGTATTCACTTCAAAACAAGAGAAGGAACAATCCGAAGGGATTGCATACCAGTGGAACTACATGGTTGAAAACCAGTATGGAAATGATGGAATGAAAGCGGGAAGCTGTTCAAACAGAGCTGAATCGTCGCCTCTAAATGACAAGACTAAATCATTAGTGTTGGTTAACTATTTTGGGTCAGTTCCCATTAAGCAGCTCTCATGTCAATTCAATTCTGAGGATTTGGTTAGCATGCTTAACACTTGCTATGGTGCTGCTGGAAATCGATGGTCAAATTTTGTTGCGGTTGATTTTTACAAGAGGAGTGGAGGAGGGGGATCATTTCAAGCTACAGACACTCTCAATGGAGAACTCATATGTGGATGTAACGATGTCCATGCATGTGTGGTAAGCTATGAAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

409

Amino Acids

45.35

Weight (kDa)

5.03

Isoelectric Point (pI)

36.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC_cat PF26178 120 - 403 7.1e-149 PI-PLC-like catalytic domain
PI-PLC_X PF26146 151 - 374 1.6e-23 PI-PLC X
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 226
AccB1I GGYRCC 1 cut(s) 226
AccB7I CCANNNNNTGG 1 cut(s) 223
AciI CCGC 4 cut(s) 140, 785, 923, 1109
AclWI GGATC 1 cut(s) 1150
AcoI YGGCCR 1 cut(s) 782
AcsI RAATTY 1 cut(s) 1099
AfaI GTAC 2 cut(s) 228, 290
AfiI CCNNNNNNNGG 4 cut(s) 223, 252, 349, 770
AjnI CCWGG 2 cut(s) 229, 649
Alw21I GWGCWC 1 cut(s) 119
AlwI GGATC 1 cut(s) 1150
AoxI GGCC 1 cut(s) 782
ApeKI GCWGC 3 cut(s) 722, 1018, 1079
ApoI RAATTY 1 cut(s) 1099
Asp700I GAANNNNTTC 1 cut(s) 931
Asp718I GGTACC 1 cut(s) 226
AspS9I GGNCC 1 cut(s) 243
AsuHPI GGTGA 1 cut(s) 253
AvaII GGWCC 1 cut(s) 243
BanI GGYRCC 1 cut(s) 226
BanII GRGCYC 1 cut(s) 514
BauI CACGAG 1 cut(s) 450
BbsI GAAGAC 2 cut(s) 11, 480
Bbv12I GWGCWC 1 cut(s) 119
BbvI GCAGC 3 cut(s) 709, 1030, 1066
BccI CCATC 4 cut(s) 83, 439, 905, 1086
BcgI CGANNNNNNTGC 4 cut(s) 848, 882, 1184, 1218
BciT130I CCWGG 2 cut(s) 231, 651
BfaI CTAG 1 cut(s) 35
BfmI CTRYAG 3 cut(s) 356, 606, 1155
BisI GCNGC 4 cut(s) 723, 785, 1019, 1080
BlsI GCNGC 4 cut(s) 724, 786, 1020, 1081
Bme1390I CCNGG 2 cut(s) 231, 651
Bme18I GGWCC 1 cut(s) 243
BmgT120I GGNCC 1 cut(s) 243
BmiI GGNNCC 3 cut(s) 181, 228, 245
BmrFI CCNGG 2 cut(s) 231, 651
BpiI GAAGAC 2 cut(s) 11, 480
BpmI CTGGAG 1 cut(s) 166
Bsa29I ATCGAT 1 cut(s) 1090
BsaJI CCNNGG 2 cut(s) 165, 649
BsaWI WCCGGW 1 cut(s) 440
BsaXI ACNNNNNCTCC 2 cut(s) 645, 675
Bsc4I CCNNNNNNNGG 4 cut(s) 223, 252, 349, 770
Bse1I ACTGG 4 cut(s) 300, 746, 874, 898
Bse3DI GCAATG 1 cut(s) 306
BseBI CCWGG 2 cut(s) 231, 651
BseCI ATCGAT 1 cut(s) 1090
BseDI CCNNGG 2 cut(s) 165, 649
BseGI GGATG 1 cut(s) 1193
BseLI CCNNNNNNNGG 4 cut(s) 223, 252, 349, 770
BseMI GCAATG 1 cut(s) 306
BseMII CTCAG 1 cut(s) 1032
BseNI ACTGG 4 cut(s) 300, 746, 874, 898
BseRI GAGGAG 2 cut(s) 1141, 1149
BseXI GCAGC 3 cut(s) 709, 1030, 1066
BshFI GGCC 1 cut(s) 784
BshNI GGYRCC 1 cut(s) 226
BshVI ATCGAT 1 cut(s) 1090
BsiHKAI GWGCWC 1 cut(s) 119
BsiSI CCGG 2 cut(s) 441, 726
BslFI GGGAC 2 cut(s) 229, 431
BslI CCNNNNNNNGG 4 cut(s) 223, 252, 349, 770
BsmFI GGGAC 2 cut(s) 229, 431
BsnI GGCC 1 cut(s) 784
Bsp1286I GDGCHC 2 cut(s) 119, 514
Bsp143I GATC 3 cut(s) 109, 194, 1142
BspACI CCGC 4 cut(s) 140, 785, 923, 1109
BspANI GGCC 1 cut(s) 784
BspCNI CTCAG 1 cut(s) 1033
BspDI ATCGAT 1 cut(s) 1090
BspLI GGNNCC 3 cut(s) 181, 228, 245
BspPI GGATC 1 cut(s) 1150
BspT107I GGYRCC 1 cut(s) 226
BsrDI GCAATG 1 cut(s) 306
BsrI ACTGG 4 cut(s) 300, 746, 874, 898
BssECI CCNNGG 2 cut(s) 165, 649
BssMI GATC 3 cut(s) 109, 194, 1142
BssSI CACGAG 1 cut(s) 450
Bst2BI CACGAG 1 cut(s) 450
Bst2UI CCWGG 2 cut(s) 231, 651
Bst4CI ACNGT 2 cut(s) 96, 152
BstC8I GCNNGC 4 cut(s) 82, 101, 603, 1058
BstDEI CTNAG 3 cut(s) 106, 807, 1041
BstF5I GGATG 1 cut(s) 1193
BstKTI GATC 3 cut(s) 112, 197, 1145
BstMBI GATC 3 cut(s) 109, 194, 1142
BstNI CCWGG 2 cut(s) 231, 651
BstNSI RCATGY 3 cut(s) 763, 1060, 1209
BstSCI CCNGG 2 cut(s) 229, 649
BstSFI CTRYAG 3 cut(s) 356, 606, 1155
BstV1I GCAGC 3 cut(s) 709, 1030, 1066
BstV2I GAAGAC 2 cut(s) 11, 480
Bsu15I ATCGAT 1 cut(s) 1090
BsuRI GGCC 1 cut(s) 784
BsuTUI ATCGAT 1 cut(s) 1090
BtsCI GGATG 1 cut(s) 1193
BtsI GCAGTG 1 cut(s) 588
BtsIMutI CAGTG 4 cut(s) 101, 119, 588, 881
Cac8I GCNNGC 4 cut(s) 82, 101, 603, 1058
Cfr13I GGNCC 1 cut(s) 243
ClaI ATCGAT 1 cut(s) 1090
CsiI ACCWGGT 1 cut(s) 229
Csp6I GTAC 2 cut(s) 227, 289
CspCI CAANNNNNGTGG 2 cut(s) 1111, 1146
CviAII CATG 9 cut(s) 66, 252, 581, 760, 886, 1026, 1057, 1202, 1206
CviQI GTAC 2 cut(s) 227, 289
DdeI CTNAG 3 cut(s) 106, 807, 1041
DpnI GATC 3 cut(s) 111, 196, 1144
DpnII GATC 3 cut(s) 109, 194, 1142
EaeI YGGCCR 1 cut(s) 782
Eco24I GRGCYC 1 cut(s) 514
Eco32I GATATC 1 cut(s) 753
Eco47I GGWCC 1 cut(s) 243
EcoRII CCWGG 2 cut(s) 229, 649
EcoRV GATATC 1 cut(s) 753
EcoT22I ATGCAT 2 cut(s) 391, 1207
EcoT38I GRGCYC 1 cut(s) 514
FaeI CATG 9 cut(s) 69, 255, 584, 763, 889, 1029, 1060, 1205, 1209
FaqI GGGAC 2 cut(s) 229, 431
FatI CATG 9 cut(s) 65, 251, 580, 759, 885, 1025, 1056, 1201, 1205
FauI CCCGC 2 cut(s) 133, 916
FauNDI CATATG 1 cut(s) 1181
Fnu4HI GCNGC 4 cut(s) 723, 785, 1019, 1080
FokI GGATG 1 cut(s) 1200
FriOI GRGCYC 1 cut(s) 514
Fsp4HI GCNGC 4 cut(s) 723, 785, 1019, 1080
FspBI CTAG 1 cut(s) 35
GluI GCNGC 4 cut(s) 723, 785, 1019, 1080
GsuI CTGGAG 1 cut(s) 166
HaeIII GGCC 1 cut(s) 784
HapII CCGG 2 cut(s) 441, 726
Hin1II CATG 9 cut(s) 69, 255, 584, 763, 889, 1029, 1060, 1205, 1209
HincII GTYRAC 1 cut(s) 991
HindII GTYRAC 1 cut(s) 991
HinfI GANTC 4 cut(s) 10, 444, 466, 947
HpaI GTTAAC 1 cut(s) 991
HpaII CCGG 2 cut(s) 441, 726
HphI GGTGA 1 cut(s) 253
Hpy166II GTNNAC 1 cut(s) 991
Hpy188I TCNGA 5 cut(s) 88, 169, 750, 859, 1042
Hpy188III TCNNGA 4 cut(s) 183, 463, 470, 776
Hpy8I GTNNAC 1 cut(s) 991
Hpy99I CGWCG 2 cut(s) 124, 955
HpyAV CCTTC 6 cut(s) 35, 416, 469, 574, 841, 854
HpyCH4III ACNGT 2 cut(s) 96, 152
HpyCH4V TGCA 5 cut(s) 99, 389, 593, 869, 1205
HpyF3I CTNAG 3 cut(s) 106, 807, 1041
Hsp92II CATG 9 cut(s) 69, 255, 584, 763, 889, 1029, 1060, 1205, 1209
KpnI GGTACC 1 cut(s) 230
KspAI GTTAAC 1 cut(s) 991
Kzo9I GATC 3 cut(s) 109, 194, 1142
LmnI GCTCC 3 cut(s) 89, 185, 697
Lsp1109I GCAGC 3 cut(s) 709, 1030, 1066
MabI ACCWGGT 1 cut(s) 229
MaeI CTAG 1 cut(s) 35
MaeIII GTNAC 6 cut(s) 259, 454, 481, 661, 802, 1190
MalI GATC 3 cut(s) 111, 196, 1144
MboI GATC 3 cut(s) 109, 194, 1142
MboII GAAGA 2 cut(s) 16, 485
MfeI CAATTG 1 cut(s) 238
MhlI GDGCHC 2 cut(s) 119, 514
MluCI AATT 7 cut(s) 212, 238, 364, 657, 1031, 1036, 1099
MlyI GAGTC 2 cut(s) 4, 453
Mph1103I ATGCAT 2 cut(s) 391, 1207
MroXI GAANNNNTTC 1 cut(s) 931
MseI TTAA 5 cut(s) 329, 495, 990, 1014, 1062
MslI CAYNNNNRTG 2 cut(s) 874, 1206
MspA1I CMGCKG 1 cut(s) 787
MspI CCGG 2 cut(s) 441, 726
MspR9I CCNGG 2 cut(s) 231, 651
MunI CAATTG 1 cut(s) 238
MvaI CCWGG 2 cut(s) 231, 651
NdeI CATATG 1 cut(s) 1181
NdeII GATC 3 cut(s) 109, 194, 1142
NlaIII CATG 9 cut(s) 69, 255, 584, 763, 889, 1029, 1060, 1205, 1209
NlaIV GGNNCC 3 cut(s) 181, 228, 245
NmuCI GTSAC 4 cut(s) 259, 481, 661, 802
NsiI ATGCAT 2 cut(s) 391, 1207
NspI RCATGY 3 cut(s) 763, 1060, 1209
PaeI GCATGC 1 cut(s) 1060
PdmI GAANNNNTTC 1 cut(s) 931
PfeI GAWTC 2 cut(s) 466, 947
PflFI GACNNNGTC 1 cut(s) 479
PflMI CCANNNNNTGG 1 cut(s) 223
PkrI GCNGC 4 cut(s) 724, 786, 1020, 1081
PleI GAGTC 2 cut(s) 4, 452
PpsI GAGTC 2 cut(s) 4, 452
Psp6I CCWGG 2 cut(s) 229, 649
PspGI CCWGG 2 cut(s) 229, 649
PspN4I GGNNCC 3 cut(s) 181, 228, 245
PspPI GGNCC 1 cut(s) 243
PsyI GACNNNGTC 1 cut(s) 479
RsaI GTAC 2 cut(s) 228, 290
RsaNI GTAC 2 cut(s) 227, 289
RseI CAYNNNNRTG 2 cut(s) 874, 1206
SaqAI TTAA 5 cut(s) 329, 495, 990, 1014, 1062
SatI GCNGC 4 cut(s) 723, 785, 1019, 1080
Sau3AI GATC 3 cut(s) 109, 194, 1142
Sau96I GGNCC 1 cut(s) 243
SchI GAGTC 2 cut(s) 4, 453
ScrFI CCNGG 2 cut(s) 231, 651
SduI GDGCHC 2 cut(s) 119, 514
SexAI ACCWGGT 1 cut(s) 229
SfcI CTRYAG 3 cut(s) 356, 606, 1155
SinI GGWCC 1 cut(s) 243
SmiMI CAYNNNNRTG 2 cut(s) 874, 1206
SphI GCATGC 1 cut(s) 1060
Sse9I AATT 7 cut(s) 212, 238, 364, 657, 1031, 1036, 1099
SsiI CCGC 4 cut(s) 140, 785, 923, 1109
SspMI CTAG 1 cut(s) 35
StyD4I CCNGG 2 cut(s) 229, 649
TaaI ACNGT 2 cut(s) 96, 152
TaqI TCGA 3 cut(s) 119, 420, 1090
TasI AATT 7 cut(s) 212, 238, 364, 657, 1031, 1036, 1099
TauI GCSGC 1 cut(s) 787
TfiI GAWTC 2 cut(s) 466, 947
Tru1I TTAA 5 cut(s) 329, 495, 990, 1014, 1062
Tru9I TTAA 5 cut(s) 329, 495, 990, 1014, 1062
TscAI CASTG 4 cut(s) 101, 119, 595, 881
TseFI GTSAC 4 cut(s) 259, 481, 661, 802
TseI GCWGC 3 cut(s) 722, 1018, 1079
Tsp45I GTSAC 4 cut(s) 259, 481, 661, 802
TspDTI ATGAA 5 cut(s) 17, 54, 636, 749, 932
TspGWI ACGGA 3 cut(s) 137, 141, 714
TspRI CASTG 4 cut(s) 101, 119, 595, 881
Tth111I GACNNNGTC 1 cut(s) 479
Van91I CCANNNNNTGG 1 cut(s) 223
VpaK11BI GGWCC 1 cut(s) 243
XapI RAATTY 1 cut(s) 1099
XceI RCATGY 3 cut(s) 763, 1060, 1209
XmnI GAANNNNTTC 1 cut(s) 931
XspI CTAG 1 cut(s) 35
Zsp2I ATGCAT 2 cut(s) 391, 1207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.