MD09G1074600.v1.1

Pollen Ole e 1 allergen and extensin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001665
Physical Location & Seq
Forward (+)
5191063 .. 5192251
1189 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1074600.v1.1.491

Sequence Viewer

Length: 687 bp
ATGTCTTGGTTTCAAACAGTTATCTTTCTGGTTACTACTCTTACATTTCATGCTCCTTCAGAGGCTAGCTATGAGGAGATCCAGCCTTCTGCAGTTGTTGTAGGTACTGTCTACTGTGACACATGTTTCCAAGAGGATTTCTCAAATACCAGCCATTTCATCTCAGGGGCTTCTGTTGGTGTGGAATGCAAAGATGGGACTTCAACAAAACCAAGTTTCAACGCACAAGTGAAAACAAACAGCCATGGGCAATTCAAAGTCCATCTGCCCTTCTCAGTGAGCAAAGATGTCAAGAAAATTGAAGGATGCTCCGTGAAACTAATCCGCAGCAGTGAACCCTACTGTGCTGTGGCCTCATCAACCACCTCATCTTCCCTACACCTCAAGGCAAGAAAGCAAGGGACTCACATATTCTCTGCTGGTTTCTTCACCTTCAAGCCTCTCAAGCAACCCAGCTTATGCAACCAGAAACCCAGCATTCAGAATTCCAAGAAATTAAATTCCCAGAAGTTCTCAGAGATGGCATTCCCGTCCTCCTCTCAGGTCTCCCAATCAATCTCCCAGGACTCCCAGGTCTCCCAGGACTCCCAGGCCTCCCAGGCCTCCCAGGACTTCCAATATCCCTTGCTGCTGAAAAAACTTCATCTAAGTCCTCCAACTCCAAAACTTCAAAGAATGGGCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

229

Amino Acids

25.02

Weight (kDa)

9.11

Isoelectric Point (pI)

50.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 33 - 122 2.4e-21 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 572
AccI GTMKAC 1 cut(s) 111
AciI CCGC 1 cut(s) 325
AclWI GGATC 1 cut(s) 73
AcsI RAATTY 2 cut(s) 484, 499
AcuI CTGAAG 1 cut(s) 42
AfaI GTAC 1 cut(s) 106
AflIII ACRYGT 1 cut(s) 122
AgsI TTSAA 7 cut(s) 14, 204, 220, 256, 302, 436, 671
AjnI CCWGG 6 cut(s) 561, 570, 579, 588, 597, 606
AluBI AGCT 2 cut(s) 69, 456
AluI AGCT 2 cut(s) 69, 456
Alw26I GTCTC 2 cut(s) 550, 580
AlwI GGATC 1 cut(s) 73
AoxI GGCC 3 cut(s) 351, 591, 600
ApeKI GCWGC 2 cut(s) 327, 628
ApoI RAATTY 2 cut(s) 484, 499
AsuHPI GGTGA 1 cut(s) 421
AsuNHI GCTAGC 1 cut(s) 65
BbvI GCAGC 2 cut(s) 339, 615
BccI CCATC 3 cut(s) 188, 270, 514
BciT130I CCWGG 6 cut(s) 563, 572, 581, 590, 599, 608
BcoDI GTCTC 2 cut(s) 550, 580
BfaI CTAG 2 cut(s) 66, 685
BfmI CTRYAG 1 cut(s) 90
BglI GCCNNNNNGGC 1 cut(s) 599
BisI GCNGC 2 cut(s) 328, 629
BlsI GCNGC 2 cut(s) 329, 630
Bme1390I CCNGG 6 cut(s) 563, 572, 581, 590, 599, 608
BmrFI CCNGG 6 cut(s) 563, 572, 581, 590, 599, 608
BmsI GCATC 1 cut(s) 296
BmtI GCTAGC 1 cut(s) 69
BplI GAGNNNNNCTC 2 cut(s) 125, 157
BpuEI CTTGAG 2 cut(s) 368, 428
BsaI GGTCTC 2 cut(s) 550, 580
BsaJI CCNNGG 7 cut(s) 244, 561, 570, 579, 588, 597, 606
BseBI CCWGG 6 cut(s) 563, 572, 581, 590, 599, 608
BseDI CCNNGG 7 cut(s) 244, 561, 570, 579, 588, 597, 606
BseGI GGATG 1 cut(s) 311
BseMII CTCAG 4 cut(s) 177, 288, 528, 554
BseRI GAGGAG 2 cut(s) 89, 526
BseXI GCAGC 2 cut(s) 339, 615
BseYI CCCAGC 2 cut(s) 452, 473
BshFI GGCC 3 cut(s) 353, 593, 602
BslFI GGGAC 2 cut(s) 211, 415
BsmAI GTCTC 2 cut(s) 550, 580
BsmFI GGGAC 2 cut(s) 211, 415
BsmI GAATGC 3 cut(s) 191, 477, 524
BsnI GGCC 3 cut(s) 353, 593, 602
Bso31I GGTCTC 2 cut(s) 550, 580
Bsp143I GATC 1 cut(s) 78
Bsp19I CCATGG 1 cut(s) 244
BspACI CCGC 1 cut(s) 325
BspANI GGCC 3 cut(s) 353, 593, 602
BspCNI CTCAG 4 cut(s) 176, 287, 527, 553
BspMAI CTGCAG 1 cut(s) 94
BspOI GCTAGC 1 cut(s) 69
BspPI GGATC 1 cut(s) 73
BspTNI GGTCTC 2 cut(s) 550, 580
BssECI CCNNGG 7 cut(s) 244, 561, 570, 579, 588, 597, 606
BssMI GATC 1 cut(s) 78
BssT1I CCWWGG 1 cut(s) 244
Bst2UI CCWGG 6 cut(s) 563, 572, 581, 590, 599, 608
Bst4CI ACNGT 4 cut(s) 19, 109, 116, 344
BstC8I GCNNGC 1 cut(s) 67
BstDEI CTNAG 5 cut(s) 163, 274, 514, 540, 647
BstDSI CCRYGG 1 cut(s) 244
BstF5I GGATG 1 cut(s) 311
BstKTI GATC 1 cut(s) 81
BstMAI GTCTC 2 cut(s) 550, 580
BstMBI GATC 1 cut(s) 78
BstMWI GCNNNNNNNGC 2 cut(s) 445, 599
BstNI CCWGG 6 cut(s) 563, 572, 581, 590, 599, 608
BstNSI RCATGY 1 cut(s) 126
BstSCI CCNGG 6 cut(s) 561, 570, 579, 588, 597, 606
BstSFI CTRYAG 1 cut(s) 90
BstV1I GCAGC 2 cut(s) 339, 615
BstX2I RGATCY 1 cut(s) 78
BstYI RGATCY 1 cut(s) 78
BsuRI GGCC 3 cut(s) 353, 593, 602
BtgI CCRYGG 1 cut(s) 244
BtsCI GGATG 1 cut(s) 311
BtsI GCAGTG 1 cut(s) 337
BtsIMutI CAGTG 2 cut(s) 282, 337
Cac8I GCNNGC 1 cut(s) 67
Csp6I GTAC 1 cut(s) 105
CviAII CATG 3 cut(s) 50, 123, 245
CviQI GTAC 1 cut(s) 105
DdeI CTNAG 5 cut(s) 163, 274, 514, 540, 647
DpnI GATC 1 cut(s) 80
DpnII GATC 1 cut(s) 78
DrdI GACNNNNNNGTC 1 cut(s) 572
DseDI GACNNNNNNGTC 1 cut(s) 572
Eco130I CCWWGG 1 cut(s) 244
Eco147I AGGCCT 2 cut(s) 593, 602
Eco31I GGTCTC 2 cut(s) 550, 580
Eco57I CTGAAG 1 cut(s) 42
EcoRI GAATTC 1 cut(s) 484
EcoRII CCWGG 6 cut(s) 561, 570, 579, 588, 597, 606
EcoT14I CCWWGG 1 cut(s) 244
ErhI CCWWGG 1 cut(s) 244
FaeI CATG 3 cut(s) 53, 126, 248
FaiI YATR 6 cut(s) 51, 72, 124, 246, 410, 460
FaqI GGGAC 2 cut(s) 211, 415
FatI CATG 3 cut(s) 49, 122, 244
FblI GTMKAC 1 cut(s) 111
Fnu4HI GCNGC 2 cut(s) 328, 629
FokI GGATG 1 cut(s) 318
Fsp4HI GCNGC 2 cut(s) 328, 629
FspBI CTAG 2 cut(s) 66, 685
GluI GCNGC 2 cut(s) 328, 629
GsaI CCCAGC 2 cut(s) 456, 477
HaeIII GGCC 3 cut(s) 353, 593, 602
Hin1II CATG 3 cut(s) 53, 126, 248
HinfI GANTC 3 cut(s) 403, 566, 584
HphI GGTGA 1 cut(s) 421
Hpy166II GTNNAC 2 cut(s) 112, 335
Hpy188I TCNGA 3 cut(s) 61, 483, 517
Hpy188III TCNNGA 1 cut(s) 292
Hpy8I GTNNAC 2 cut(s) 112, 335
HpyAV CCTTC 5 cut(s) 66, 96, 280, 296, 442
HpyCH4III ACNGT 4 cut(s) 19, 109, 116, 344
HpyCH4V TGCA 3 cut(s) 92, 189, 462
HpyF10VI GCNNNNNNNGC 2 cut(s) 445, 599
HpyF3I CTNAG 5 cut(s) 163, 274, 514, 540, 647
Hsp92II CATG 3 cut(s) 53, 126, 248
Kzo9I GATC 1 cut(s) 78
LmnI GCTCC 2 cut(s) 58, 314
Lsp1109I GCAGC 2 cut(s) 339, 615
LweI GCATC 1 cut(s) 296
MaeI CTAG 2 cut(s) 66, 685
MaeIII GTNAC 2 cut(s) 31, 116
MalI GATC 1 cut(s) 80
MboI GATC 1 cut(s) 78
MboII GAAGA 2 cut(s) 363, 418
MflI RGATCY 1 cut(s) 78
MluCI AATT 5 cut(s) 251, 297, 484, 494, 499
MlyI GAGTC 3 cut(s) 397, 560, 578
MmeI TCCRAC 1 cut(s) 680
MseI TTAA 1 cut(s) 497
MspR9I CCNGG 6 cut(s) 563, 572, 581, 590, 599, 608
Mva1269I GAATGC 3 cut(s) 191, 477, 524
MvaI CCWGG 6 cut(s) 563, 572, 581, 590, 599, 608
MwoI GCNNNNNNNGC 2 cut(s) 445, 599
NcoI CCATGG 1 cut(s) 244
NdeII GATC 1 cut(s) 78
NheI GCTAGC 1 cut(s) 65
NlaIII CATG 3 cut(s) 53, 126, 248
NmuCI GTSAC 1 cut(s) 116
NspI RCATGY 1 cut(s) 126
PceI AGGCCT 2 cut(s) 593, 602
PciI ACATGT 1 cut(s) 122
PctI GAATGC 3 cut(s) 191, 477, 524
PkrI GCNGC 2 cut(s) 329, 630
PleI GAGTC 3 cut(s) 397, 560, 578
PpsI GAGTC 3 cut(s) 397, 560, 578
PscI ACATGT 1 cut(s) 122
Psp6I CCWGG 6 cut(s) 561, 570, 579, 588, 597, 606
PspFI CCCAGC 2 cut(s) 452, 473
PspGI CCWGG 6 cut(s) 561, 570, 579, 588, 597, 606
PstI CTGCAG 1 cut(s) 94
PsuI RGATCY 1 cut(s) 78
RsaI GTAC 1 cut(s) 106
RsaNI GTAC 1 cut(s) 105
SaqAI TTAA 1 cut(s) 497
SatI GCNGC 2 cut(s) 328, 629
Sau3AI GATC 1 cut(s) 78
SchI GAGTC 3 cut(s) 397, 560, 578
ScrFI CCNGG 6 cut(s) 563, 572, 581, 590, 599, 608
SetI ASST 8 cut(s) 71, 106, 368, 384, 434, 458, 546, 576
SfaNI GCATC 1 cut(s) 296
SfcI CTRYAG 1 cut(s) 90
SfiI GGCCNNNNNGGCC 1 cut(s) 599
SmlI CTYRAG 2 cut(s) 383, 443
SmoI CTYRAG 2 cut(s) 383, 443
Sse9I AATT 5 cut(s) 251, 297, 484, 494, 499
SseBI AGGCCT 2 cut(s) 593, 602
SsiI CCGC 1 cut(s) 325
SspMI CTAG 2 cut(s) 66, 685
StuI AGGCCT 2 cut(s) 593, 602
StyD4I CCNGG 6 cut(s) 561, 570, 579, 588, 597, 606
StyI CCWWGG 1 cut(s) 244
TaaI ACNGT 4 cut(s) 19, 109, 116, 344
TasI AATT 5 cut(s) 251, 297, 484, 494, 499
Tru1I TTAA 1 cut(s) 497
Tru9I TTAA 1 cut(s) 497
TscAI CASTG 2 cut(s) 282, 337
TseFI GTSAC 1 cut(s) 116
TseI GCWGC 2 cut(s) 327, 628
Tsp45I GTSAC 1 cut(s) 116
TspDTI ATGAA 3 cut(s) 38, 148, 632
TspGWI ACGGA 1 cut(s) 301
TspRI CASTG 2 cut(s) 282, 337
XapI RAATTY 2 cut(s) 484, 499
XceI RCATGY 1 cut(s) 126
XmiI GTMKAC 1 cut(s) 111
XspI CTAG 2 cut(s) 66, 685
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.