RLG00000021491

Pollen Ole e 1 allergen and extensin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001665
Physical Location & Seq
Reverse (-)
75866198 .. 75867480
1283 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021491

Sequence Viewer

Length: 1122 bp
ATGTCTTGGTTTCTAACAATATTCTTCCTCATTAGTTTCACATTTAGTACTCCTTCAGAGGCTAACCATGAAAAGAAGCTTCAACCTGCTGTGGTTATAGGCAAAGTCTACTGTGAGACATGTTTCCAAGATGGGTTCTCAAAATCCAGCCACTTCATTTCAGGGGCTTCTGTTGGTGTGGAGTGCAAAGATCAGACTTCATCAAAACCCAGCTTTCAAGCCCAAGTGAAGACAGATAACCAAGGAGTGTTCAAAGTCCAATTGCCTTTCTCAGTGAGCAAACATGTGAAGAAAATTGAAGGGTGTTCTGTGAAGCTAATTCGCAGCAGCGAACCGTATTGTGCTGTGGCCTCAGCAGCATCTTCGTCTTCACTTCACCTCAAATCAAGGAAGCAAGGAACTCACATTTTCTCAGCTGGGTTTTTCACTTTCAAGCCCCTAAACCAACCCAACCTATGTAACCAAAAACCAAGCCTCGTCCAAAACAACAAGAAGGAAGTTATCACTAATTCCAAGAAACTCTCATTTTCTCCAACTGAGGATTTGACATTTCCCTCACCAGTTCAAGAACCCAGCTTTCCTGGTGTTCCTTCTCTCCCTAGACTTCCTTATCTTCCACCATTACCACAACTCCCTCCTCTCCCAGGCCTTCCAGGACTCCCAGGTCTTCCAGGACTTCCGGGAATAGGAATTCCGACTTTACCCCCCATCCCAGGAAAAGTAACAGAGTCCCATTCCAATACCAAAACTACAACTACCTCTTCAAAGACAGAGAGGCACCTAACTGAAGAAGAAAAACTAGCCCGCCCAGATTTTTTCTTCCCACCGAACCCTCTCAATCCACCATCAATTTTGCCTCCAAACCCTCTCAATCCACCATCAATTTTGCCTCCAAACCCTCTTCTGCCAAGCCCACCTCCACTTATCCCAAACCCATTTCAGCCTCCACCAGCACCATTGATTCCAAATTTGCCACCATTGCCTAGCTTGCCACCACTACCAAGCTTAACTCCACCATCTCCACCACCACCATCATTCCCAACAATCCCTCCATTGCCTCCATTCCCATTCCCACCTGTACCTCTCATCCCTGGAATCCCCCCTGCTTCTTCCTCTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

374

Amino Acids

40.02

Weight (kDa)

9.34

Isoelectric Point (pI)

70.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 32 - 121 3.7e-20 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 663
Acc36I ACCTGC 1 cut(s) 94
AccB1I GGYRCC 1 cut(s) 777
AccI GTMKAC 1 cut(s) 108
AciI CCGC 1 cut(s) 805
AcsI RAATTY 2 cut(s) 690, 967
AcuI CTGAAG 2 cut(s) 39, 807
AfaI GTAC 2 cut(s) 49, 1080
AfiI CCNNNNNNNGG 3 cut(s) 644, 686, 713
AflIII ACRYGT 2 cut(s) 119, 283
AgsI TTSAA 7 cut(s) 83, 218, 253, 299, 433, 566, 765
AjnI CCWGG 7 cut(s) 580, 643, 652, 661, 670, 712, 1090
AluBI AGCT 7 cut(s) 79, 213, 316, 416, 576, 987, 1005
AluI AGCT 7 cut(s) 79, 213, 316, 416, 576, 987, 1005
Alw26I GTCTC 1 cut(s) 110
AoxI GGCC 2 cut(s) 348, 646
ApeKI GCWGC 3 cut(s) 324, 327, 356
ApoI RAATTY 2 cut(s) 690, 967
AsuC2I CCSGG 1 cut(s) 681
AsuHPI GGTGA 2 cut(s) 368, 549
BanI GGYRCC 1 cut(s) 777
BbsI GAAGAC 3 cut(s) 236, 360, 659
BbvCI CCTCAGC 1 cut(s) 352
BbvI GCAGC 3 cut(s) 336, 339, 368
BccI CCATC 6 cut(s) 125, 716, 853, 886, 1024, 1039
BcgI CGANNNNNNTGC 2 cut(s) 345, 379
BciT130I CCWGG 7 cut(s) 582, 645, 654, 663, 672, 714, 1092
BcnI CCSGG 1 cut(s) 681
BcoDI GTCTC 1 cut(s) 110
BfaI CTAG 3 cut(s) 600, 800, 984
BfuAI ACCTGC 1 cut(s) 94
BisI GCNGC 3 cut(s) 325, 328, 357
BlsI GCNGC 3 cut(s) 326, 329, 358
BmcAI AGTACT 1 cut(s) 49
Bme1390I CCNGG 8 cut(s) 582, 645, 654, 663, 672, 681, 714, 1092
BmiI GGNNCC 1 cut(s) 779
BmrFI CCNGG 8 cut(s) 582, 645, 654, 663, 672, 681, 714, 1092
BmsI GCATC 1 cut(s) 368
BpiI GAAGAC 3 cut(s) 236, 360, 659
Bpu10I CCTNAGC 1 cut(s) 352
BpuMI CCSGG 1 cut(s) 681
BsaJI CCNNGG 5 cut(s) 241, 643, 661, 712, 1090
BsaXI ACNNNNNCTCC 4 cut(s) 615, 645, 1033, 1063
Bsc4I CCNNNNNNNGG 3 cut(s) 644, 686, 713
Bse1I ACTGG 1 cut(s) 560
Bse3DI GCAATG 2 cut(s) 977, 1052
BseBI CCWGG 7 cut(s) 582, 645, 654, 663, 672, 714, 1092
BseDI CCNNGG 5 cut(s) 241, 643, 661, 712, 1090
BseGI GGATG 2 cut(s) 708, 1086
BseLI CCNNNNNNNGG 3 cut(s) 644, 686, 713
BseMI GCAATG 2 cut(s) 977, 1052
BseMII CTCAG 4 cut(s) 285, 366, 426, 528
BseNI ACTGG 1 cut(s) 560
BseRI GAGGAG 1 cut(s) 627
BseXI GCAGC 3 cut(s) 336, 339, 368
BseYI CCCAGC 3 cut(s) 209, 416, 572
BshFI GGCC 2 cut(s) 350, 648
BshNI GGYRCC 1 cut(s) 777
BsiSI CCGG 1 cut(s) 680
BslFI GGGAC 1 cut(s) 715
BslI CCNNNNNNNGG 3 cut(s) 644, 686, 713
BsmAI GTCTC 1 cut(s) 110
BsmFI GGGAC 1 cut(s) 715
BsnI GGCC 2 cut(s) 350, 648
Bsp143I GATC 1 cut(s) 190
BspACI CCGC 1 cut(s) 805
BspANI GGCC 2 cut(s) 350, 648
BspCNI CTCAG 4 cut(s) 284, 365, 425, 529
BspLI GGNNCC 1 cut(s) 779
BspMI ACCTGC 1 cut(s) 94
BspT107I GGYRCC 1 cut(s) 777
BsrDI GCAATG 2 cut(s) 977, 1052
BsrI ACTGG 1 cut(s) 560
BssECI CCNNGG 5 cut(s) 241, 643, 661, 712, 1090
BssMI GATC 1 cut(s) 190
BssT1I CCWWGG 1 cut(s) 241
Bst2UI CCWGG 7 cut(s) 582, 645, 654, 663, 672, 714, 1092
Bst4CI ACNGT 2 cut(s) 113, 336
Bst6I CTCTTC 2 cut(s) 766, 906
BstC8I GCNNGC 2 cut(s) 805, 989
BstDEI CTNAG 4 cut(s) 271, 352, 412, 537
BstENI CCTNNNNNAGG 1 cut(s) 642
BstF5I GGATG 2 cut(s) 708, 1086
BstKTI GATC 1 cut(s) 193
BstMAI GTCTC 1 cut(s) 110
BstMBI GATC 1 cut(s) 190
BstMWI GCNNNNNNNGC 3 cut(s) 356, 979, 988
BstNI CCWGG 7 cut(s) 582, 645, 654, 663, 672, 714, 1092
BstNSI RCATGY 2 cut(s) 123, 287
BstSCI CCNGG 8 cut(s) 580, 643, 652, 661, 670, 679, 712, 1090
BstV1I GCAGC 3 cut(s) 336, 339, 368
BstV2I GAAGAC 3 cut(s) 236, 360, 659
BsuRI GGCC 2 cut(s) 350, 648
BtsCI GGATG 2 cut(s) 708, 1086
BtsIMutI CAGTG 1 cut(s) 279
BveI ACCTGC 1 cut(s) 94
Cac8I GCNNGC 2 cut(s) 805, 989
Csp6I GTAC 2 cut(s) 48, 1079
CviAII CATG 3 cut(s) 68, 120, 284
CviQI GTAC 2 cut(s) 48, 1079
DdeI CTNAG 4 cut(s) 271, 352, 412, 537
DpnI GATC 1 cut(s) 192
DpnII GATC 1 cut(s) 190
DrdI GACNNNNNNGTC 1 cut(s) 663
DseDI GACNNNNNNGTC 1 cut(s) 663
Eam1104I CTCTTC 2 cut(s) 766, 906
EarI CTCTTC 2 cut(s) 766, 906
Eco130I CCWWGG 1 cut(s) 241
Eco147I AGGCCT 1 cut(s) 648
Eco57I CTGAAG 2 cut(s) 39, 807
EcoNI CCTNNNNNAGG 1 cut(s) 642
EcoRI GAATTC 1 cut(s) 690
EcoRII CCWGG 7 cut(s) 580, 643, 652, 661, 670, 712, 1090
EcoT14I CCWWGG 1 cut(s) 241
ErhI CCWWGG 1 cut(s) 241
FaeI CATG 3 cut(s) 71, 123, 287
FaiI YATR 5 cut(s) 69, 98, 121, 285, 457
FaqI GGGAC 1 cut(s) 715
FatI CATG 3 cut(s) 67, 119, 283
FauI CCCGC 1 cut(s) 812
FblI GTMKAC 1 cut(s) 108
Fnu4HI GCNGC 3 cut(s) 325, 328, 357
FokI GGATG 2 cut(s) 695, 1073
Fsp4HI GCNGC 3 cut(s) 325, 328, 357
FspBI CTAG 3 cut(s) 600, 800, 984
GluI GCNGC 3 cut(s) 325, 328, 357
GsaI CCCAGC 3 cut(s) 213, 420, 576
HaeIII GGCC 2 cut(s) 350, 648
HapII CCGG 1 cut(s) 680
Hin1II CATG 3 cut(s) 71, 123, 287
HindIII AAGCTT 2 cut(s) 77, 1003
HinfI GANTC 4 cut(s) 657, 728, 961, 1095
HpaII CCGG 1 cut(s) 680
HphI GGTGA 2 cut(s) 368, 549
Hpy166II GTNNAC 1 cut(s) 109
Hpy188I TCNGA 3 cut(s) 58, 195, 696
Hpy188III TCNNGA 1 cut(s) 566
Hpy8I GTNNAC 1 cut(s) 109
HpyAV CCTTC 5 cut(s) 63, 293, 487, 600, 659
HpyCH4III ACNGT 2 cut(s) 113, 336
HpyCH4V TGCA 1 cut(s) 186
HpyF10VI GCNNNNNNNGC 3 cut(s) 356, 979, 988
HpyF3I CTNAG 4 cut(s) 271, 352, 412, 537
Hsp92II CATG 3 cut(s) 71, 123, 287
Kzo9I GATC 1 cut(s) 190
Lsp1109I GCAGC 3 cut(s) 336, 339, 368
LweI GCATC 1 cut(s) 368
MaeI CTAG 3 cut(s) 600, 800, 984
MaeIII GTNAC 2 cut(s) 458, 721
MalI GATC 1 cut(s) 192
MboI GATC 1 cut(s) 190
MfeI CAATTG 1 cut(s) 260
MluCI AATT 8 cut(s) 260, 294, 318, 508, 690, 849, 882, 967
MlyI GAGTC 2 cut(s) 651, 737
MmeI TCCRAC 2 cut(s) 557, 719
MseI TTAA 1 cut(s) 1007
MspA1I CMGCKG 1 cut(s) 416
MspI CCGG 1 cut(s) 680
MspR9I CCNGG 8 cut(s) 582, 645, 654, 663, 672, 681, 714, 1092
MunI CAATTG 1 cut(s) 260
MvaI CCWGG 7 cut(s) 582, 645, 654, 663, 672, 714, 1092
MwoI GCNNNNNNNGC 3 cut(s) 356, 979, 988
NciI CCSGG 1 cut(s) 681
NdeII GATC 1 cut(s) 190
NlaIII CATG 3 cut(s) 71, 123, 287
NlaIV GGNNCC 1 cut(s) 779
NspI RCATGY 2 cut(s) 123, 287
PceI AGGCCT 1 cut(s) 648
PciI ACATGT 2 cut(s) 119, 283
PfeI GAWTC 2 cut(s) 961, 1095
PfoI TCCNGGA 3 cut(s) 652, 670, 679
PkrI GCNGC 3 cut(s) 326, 329, 358
PleI GAGTC 2 cut(s) 651, 736
PpsI GAGTC 2 cut(s) 651, 736
PscI ACATGT 2 cut(s) 119, 283
Psp6I CCWGG 7 cut(s) 580, 643, 652, 661, 670, 712, 1090
PspFI CCCAGC 3 cut(s) 209, 416, 572
PspGI CCWGG 7 cut(s) 580, 643, 652, 661, 670, 712, 1090
PspN4I GGNNCC 1 cut(s) 779
PvuII CAGCTG 1 cut(s) 416
RsaI GTAC 2 cut(s) 49, 1080
RsaNI GTAC 2 cut(s) 48, 1079
SaqAI TTAA 1 cut(s) 1007
SatI GCNGC 3 cut(s) 325, 328, 357
Sau3AI GATC 1 cut(s) 190
ScaI AGTACT 1 cut(s) 49
SchI GAGTC 2 cut(s) 651, 737
ScrFI CCNGG 8 cut(s) 582, 645, 654, 663, 672, 681, 714, 1092
SfaNI GCATC 1 cut(s) 368
Sse9I AATT 8 cut(s) 260, 294, 318, 508, 690, 849, 882, 967
SseBI AGGCCT 1 cut(s) 648
SsiI CCGC 1 cut(s) 805
SspI AATATT 1 cut(s) 21
SspMI CTAG 3 cut(s) 600, 800, 984
StuI AGGCCT 1 cut(s) 648
StyD4I CCNGG 8 cut(s) 580, 643, 652, 661, 670, 679, 712, 1090
StyI CCWWGG 1 cut(s) 241
TaaI ACNGT 2 cut(s) 113, 336
TasI AATT 8 cut(s) 260, 294, 318, 508, 690, 849, 882, 967
TatI WGTACW 1 cut(s) 47
TfiI GAWTC 2 cut(s) 961, 1095
Tru1I TTAA 1 cut(s) 1007
Tru9I TTAA 1 cut(s) 1007
TscAI CASTG 1 cut(s) 279
TseI GCWGC 3 cut(s) 324, 327, 356
TspDTI ATGAA 3 cut(s) 84, 145, 189
TspRI CASTG 1 cut(s) 279
XagI CCTNNNNNAGG 1 cut(s) 642
XapI RAATTY 2 cut(s) 690, 967
XceI RCATGY 2 cut(s) 123, 287
XmiI GTMKAC 1 cut(s) 108
XspI CTAG 3 cut(s) 600, 800, 984
ZrmI AGTACT 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.